LOCUS       pEGFP-IkB               5795 bp    DNA     circular     22-OCT-2014
DEFINITION  LMBP 8293.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8293
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 21-OCT-2014
            
COMMENT     VNTNAME|pEGFP-IkB|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEGFP-IkB" 0 5795 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "AgeI" "HaeII" "HincII" "HindIII" "HpaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5795 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 601 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 7 599 0 1417 0 2329 0 3087 
COMMENT            0 3095 0 3823 0 5498 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HincII" "gtyrac" 4 0 4 1372 0 2023 0 2125 0 
COMMENT            2583 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1353 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HpaI" "gttaac" 4 0 1 2583 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2561 2657 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2561..2657") 
COMMENT        #6=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2561 2657 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2561..2657)") 
COMMENT        #7=(CFSignal (CObList) "f1 ori" 33 0 1 2711 3166 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2711..3166)") 
COMMENT        #8=(CFSignal (CObList) "ampicillin resistance promoter" 30 0 0 3167 3262 
COMMENT            0 (CStringList) (CStringList) 1 1 1 1 "3167..3262") 
COMMENT        #9=(CFSignal (CObList) "5' UTR of amp, incl. RBS" 32 0 0 3263 3297 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3263..3297") 
COMMENT        #10=(CFSignal (CObList) "SV40 early promoter" 29 0 0 3318 3599 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3318..3599") 
COMMENT        #11=(CFSignal (CObList) "SV40 ori" 33 0 0 3556 3631 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3556..3631") 
COMMENT        #12=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 3691 4485 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3691..4485") 
COMMENT        #13=(CFSignal (CObList) "HSV-TK polyA" 43 0 0 4676 4745 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4676..4745") 
COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 0 5463 5789 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5463..5789") 
COMMENT        #15=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 1 568 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1..568") 
COMMENT        #16=(CFSignal (CObList) "EGFP" 4 0 0 613 1329 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "613..1329") 
COMMENT        #17=(CFSignal (CObList) "NFKBIA hybrid" 4 0 0 1396 2340 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1396..2340")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #18=(CFSignal (CObList) "Last modified: 21-OCT-2014" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #19=(CFSignal (CObList) "LMBP 8293" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #20=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #21=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pEGFP-IkB" 2) 5 
COMMENT                   "" 0 4) 
COMMENT              #22=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pEGFP-IkB'" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 8293." 
COMMENT                          1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/22/14 11:09" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5795 bp" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #31=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 20-OCT-2014" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #34=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #35=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #36=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #37=(CLinePar (CParagraph 0 (15 #18# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 21-OCT-2014") 
COMMENT                     #38=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #39=(CLinePar (CParagraph 0 (15 #19# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 8293"))) "Annotations") 
COMMENT              #40=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #41=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #42=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 613 (3 #16# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 613   End: 1329" 1) 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   613..1329" 1))) "EGFP") 
COMMENT                            #46=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1396 (3 #17# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1396  End: 2340" 1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1396..2340" 1))) "NFKBIA hybrid") 
COMMENT                            #50=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3691 (3 #12# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3691  End: 4485" 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3691..4485" 1))) 
COMMENT                                 "Tn5 neomycin resistance"))) "CDS (3 total)") 
COMMENT                     #54=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #55=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1     End: 568 " 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1..568" 1))) 
COMMENT                                 "hCMV-IE promoter and enhancer") 
COMMENT                            #59=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3318 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3318  End: 3599" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3318..3599" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #63=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #64=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3167 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3167  End: 3262" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3167..3262" 1))) 
COMMENT                                 "ampicillin resistance promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #68=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 32 (7 32 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #69=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3263 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3263  End: 3297" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3263..3297" 1))) 
COMMENT                                 "5' UTR of amp, incl. RBS"))) "RBS (1 total)") 
COMMENT                     #73=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2711 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2711  End: 3166 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2711..3166)" 1))) 
COMMENT                                 "f1 ori") 
COMMENT                            #78=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3556 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3556  End: 3631" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3556..3631" 1))) "SV40 ori") 
COMMENT                            #82=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5463 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5463  End: 5789" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5463..5789" 1))) "pMB1 ori"))) 
COMMENT                          "Replication Origin (3 total)") 
COMMENT                     #86=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #87=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2561 (3 #5# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2561  End: 2657" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2561..2657" 1))) "SV40 polyA early") 
COMMENT                            #91=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2561 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2561  End: 2657 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2561..2657)" 1))) 
COMMENT                                 "SV40 polyA late") 
COMMENT                            #95=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4676 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4676  End: 4745" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4676..4745" 1))) "HSV-TK polyA"))) 
COMMENT                          "Terminator (3 total)"))) "Feature Map") 
COMMENT              #99=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #100=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #101=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #102=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 601 " 1))))) "AgeI: 1 site") 1 
COMMENT                           5 "ACCGGT" "TGGCCA") 
COMMENT                     #103=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #104=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #105=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 1353 " 1))))) "HindIII: 1 site") 
COMMENT                           1 5 "AAGCTT" "TTCGAA") 
COMMENT                     #106=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #107=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #108=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 2583 " 1))))) "HpaI: 1 site") 3 
COMMENT                           3 "GTTAAC" "CAATTG") 
COMMENT                     #109=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #110=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 1372 " 1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 2023 " 1) 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #2# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 2125 " 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #2# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 2583 " 1))))) "HincII: 4 sites") 
COMMENT                           3 3 "GTYRAC" "CARYTG") 
COMMENT                     #115=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 7 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #116=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 599 " 1) 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 1417 " 1) 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #1# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 2329 " 1) 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #1# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 3087 " 1) 
COMMENT                                     #121=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #1# 5) 1 2 2 0 191) 
COMMENT                                           " N5: 3095 " 1) 
COMMENT                                     #122=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #1# 6) 1 2 2 0 191) 
COMMENT                                           " N6: 3823 " 1) 
COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #1# 7) 1 2 2 0 191) 
COMMENT                                           " N7: 5498 " 1))))) "HaeII: 7 sites") 
COMMENT                           5 1 "RGCGCY" "YCGCGR"))) 
COMMENT                   "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #124=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #125=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #126=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #127=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #128=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #129=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #130=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) 
COMMENT          #131=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #132=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #133=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #134=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #135=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #136=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #137=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #138=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #139=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #140=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #141=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #142=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) #143=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #144=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #145=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #146=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #147=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #148=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #149=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #150=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #151=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #152=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #153=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #154=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #155=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #156=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #157=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #158=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #159=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #160=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #161=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #162=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #163=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #164=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #165=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #166=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #167=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #168=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #169=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #170=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #171=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #172=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #173=(CWidgetStyle "Label 27" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #174=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #175=(CWidgetStyle "Label 28" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #176=(CWidgetStyle "Label 29" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #177=(CWidgetStyle "Mutation Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #178=(CWidgetStyle "Label 30" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #179=(CWidgetStyle "MSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #180=(CWidgetStyle "Label 34" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #181=(CWidgetStyle "Label 45" 1 (LOGPEN 0 0 3381657) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #182=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 39321) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #183=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 16724736) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 20" 1 "Label 21") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 21") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 30") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 
COMMENT         -3261) 1 1 0 1 1 
COMMENT        (mapper: 24.0158 -17.3222 40.3368 40.3368 0.01 10 -1 5795 5795 1 0 0) 
COMMENT        #184=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 824188960 100) 
COMMENT              (CObjectList 
COMMENT               #185=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -397 100) 
COMMENT                     (CObjectList 
COMMENT                      #186=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #130# 27598852 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27527 
COMMENT                             6.27327 1 0.0214037) 0.108252) 
COMMENT                      #187=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #127# 24203732 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "pEGFP-IkB" "@N" 1 0 0.871165 0 -10 
COMMENT                            7.01593 1.56185 Nil) 
COMMENT                      #188=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #126# 24762812 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 73 27 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "5795 bp" "@L bp" 16 
COMMENT                            0 -0.871165 0 -10.8 4.78471 1.80976 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #189=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1170 100) 
COMMENT                     (CObjectList 
COMMENT                      #190=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -1100 100) 
COMMENT                            (CObjectList 
COMMENT                             #191=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #128# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 1.4181 
COMMENT                                    2.2787 1 0.082322) 0.8 1.8 0) 
COMMENT                             #192=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #168# 14746192 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Tn5 neomycin resistance" "@N" 1 -17.2398 
COMMENT                                   -3.72563 0 -10 10.09 1.16519 #191#) 
COMMENT                             #193=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #128# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    4.83452 5.61068 1 0.082322) 0.8 1.8 0) 
COMMENT                             #194=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #168# 27836632 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "EGFP" "@N" 1 
COMMENT                                   14.3735 8.20194 0 -10 2.256 1.16519 #193#) 
COMMENT                             #195=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #128# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 3.7401 
COMMENT                                    4.76307 1 0.082322) 0.8 1.8 0) 
COMMENT                             #196=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #168# 28437664 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "NFKBIA hybrid" 
COMMENT                                   "@N" 1 14.1332 -6.46655 0 -10 5.87553 1.16519 
COMMENT                                   #195#)) (CObjectList)) 
COMMENT                      #197=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -1030 100) 
COMMENT                            (CObjectList 
COMMENT                             #198=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #165# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    2.37721 2.68248 1 0.082322) 0.8 1.8 0) 
COMMENT                             #199=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #168# 24215420 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early promoter" "@N" 1 -10.7992 
COMMENT                                   -10.2913 0 -10 8.35465 1.16519 #198#) 
COMMENT                             #200=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #165# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    5.65832 6.27319 1 0.082322) 0.8 1.8 0) 
COMMENT                             #201=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #168# 27917068 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "hCMV-IE promoter and enhancer" "@N" 1 
COMMENT                                   11.4478 14.9977 0 -10 13.065 1.16519 #200#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #202=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -889 100) 
COMMENT                            (CObjectList 
COMMENT                             #203=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #170# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    2.74202 2.84594 1 0.082322) 0.8 1.8 0) 
COMMENT                             #204=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #168# 28445652 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "ampicillin resistance promoter" "@N" 1 
COMMENT                                   -10.3539 -14.1727 0 -10 11.8254 1.16519 #203#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #205=(CGroupWidget 
COMMENT                            (CWidget 32 (7 32 0) 1 2 0 0 Nil -1170 100) 
COMMENT                            (CObjectList 
COMMENT                             #206=(CScratch 
COMMENT                                   (CWidget 0 (3 #9# 0) 1 2 0 0 #145# 854 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 1 2.74202 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #207=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #169# 28444356 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' UTR of amp, incl. RBS" "@N" 1 -9.80134 
COMMENT                                   -12.4303 0 -10 9.69338 1.16519 #206#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #208=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -116 100) 
COMMENT                            (CObjectList 
COMMENT                             #209=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #140# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    2.84594 3.33956 1 0.082322) 0.8 1.8 1) 
COMMENT                             #210=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #168# 28446828 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "f1 ori" "@N" 1 
COMMENT                                   0.470798 -18.1353 0 -10 2.15684 1.16519 #209#) 
COMMENT                             #211=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #140# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 
COMMENT                                    0.835356 2.34257 2.42484 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #212=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #168# 27413188 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 ori" "@N" 
COMMENT                                   1 -11.0811 -8.52423 0 -10 3.56994 1.16519 
COMMENT                                   #211#) 
COMMENT                             #213=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #140# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    0.0064951 0.360478 1 0.082322) 0.8 1.8 0) 
COMMENT                             #214=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #168# 14746192 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 -4.03608 14.8242 0 -10 3.56994 1.16519 
COMMENT                                   #213#)) (CObjectList)) 
COMMENT                      #215=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -1100 100) 
COMMENT                            (CObjectList 
COMMENT                             #216=(CLine 
COMMENT                                   (CWidget 0 (3 #5# 0) 1 2 0 0 #131# 750 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 3.39694 
COMMENT                                   3.50194) 
COMMENT                             #217=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #178# 28353204 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA early" "@N" 1 7.77978 -16.1768 0 
COMMENT                                   -10 7.11509 1.16519 #216#) 
COMMENT                             #218=(CLine 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #131# 7103842 
COMMENT                                    100) (LOGPEN 0 6 16711680) 8 0.670712 
COMMENT                                   3.39694 3.50194) 
COMMENT                             #219=(CLabel (CWidget 0 (0 0) 1 2 0 0 #178# 82 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA late" "@N" 1 6.62043 -17.9191 0 
COMMENT                                   -10 6.54489 1.16519 #218#) 
COMMENT                             #220=(CLine 
COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #131# 891428905 
COMMENT                                    100) (LOGPEN 0 6 16711680) 8 0.835356 
COMMENT                                   1.13664 1.21242) 
COMMENT                             #221=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #178# 14746192 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "HSV-TK polyA" 
COMMENT                                   "@N" 1 -14.3572 5.22699 0 -10 5.87553 1.16519 
COMMENT                                   #220#)) (CObjectList))) (CObjectList)) 
COMMENT               #222=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -116 100) 
COMMENT                     (CObjectList 
COMMENT                      #223=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -960 100) 
COMMENT                            (CObjectList 
COMMENT                             #224=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #183# 879 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 5.62368 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #225=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #180# 24504176 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Age}I (601)" "@N (@S)" 5 11.4736 
COMMENT                                   11.9276 0 -10 3.66911 0.99165 #224#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #226=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -749 100) 
COMMENT                            (CObjectList 
COMMENT                             #227=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #183# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 4.80962 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #228=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #180# 24508936 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Hin}dIII (1353)" "@N (@S)" 5 16.3106 
COMMENT                                   2.98189 0 -10 4.98304 0.99165 #227#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #229=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #4# 0) 1 2 0 0 Nil -656 100) 
COMMENT                            (CObjectList 
COMMENT                             #230=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #183# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 3.47813 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #231=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #180# 27665728 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Hpa}I (2583)" "@N (@S)" 5 7.01036 
COMMENT                                   -12.94 0 -10 4.2641 0.99165 #230#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #232=(CGroupWidget 
COMMENT                            (CWidget 4 (10 #2# 0) 1 2 0 0 Nil -397 100) 
COMMENT                            (CObjectList 
COMMENT                             #233=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #182# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 4.78905 1.9 0.082322 1) 
COMMENT                             #234=(CLabel (CWidget 0 (0 0) 1 2 0 0 #181# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (1372)" "@N (@S)" 5 16.5162 
COMMENT                                   1.66101 0 -10 4.66076 0.99165 #233#) 
COMMENT                             #235=(CScratch 
COMMENT                                   (CWidget 2 (1 #2# 2) 1 2 0 0 #182# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 4.08434 1.9 0.082322 1) 
COMMENT                             #236=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #181# 28348588 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (2023)" "@N (@S)" 5 12.7152 
COMMENT                                   -8.03534 0 -10 4.66076 0.99165 #235#) 
COMMENT                             #237=(CScratch 
COMMENT                                   (CWidget 3 (1 #2# 3) 1 2 0 0 #182# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 3.97392 1.9 0.082322 1) 
COMMENT                             #238=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #181# 28439592 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (2125)" "@N (@S)" 5 12.2534 
COMMENT                                   -9.52976 0 -10 4.66076 0.99165 #237#) 
COMMENT                             #239=(CScratch 
COMMENT                                   (CWidget 4 (1 #2# 4) 1 2 0 0 #182# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 3.47813 1.9 0.082322 1) 
COMMENT                             #240=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #181# 24511368 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (2583)" "@N (@S)" 5 6.93112 
COMMENT                                   -14.3353 0 -10 4.66076 0.99165 #239#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #241=(CGroupWidget 
COMMENT                            (CWidget 7 (10 #1# 0) 1 2 0 0 Nil -819 100) 
COMMENT                            (CObjectList 
COMMENT                             #242=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #182# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 5.62584 1.9 0.082322 1) 
COMMENT                             #243=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #181# 27616304 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hae}II (599)" "@N (@S)" 5 11.1276 
COMMENT                                   13.5955 0 -10 3.99139 0.99165 #242#) 
COMMENT                             #244=(CScratch 
COMMENT                                   (CWidget 2 (1 #1# 2) 1 2 0 0 #182# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 4.74034 1.9 0.082322 1) 
COMMENT                             #245=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #181# 27837008 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hae}II (1417)" "@N (@S)" 5 16.9193 
COMMENT                                   -0.254855 0 -10 4.41284 0.99165 #244#) 
COMMENT                             #246=(CScratch 
COMMENT                                   (CWidget 3 (1 #1# 3) 1 2 0 0 #182# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 3.75309 1.9 0.082322 1) 
COMMENT                             #247=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #181# 28451352 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hae}II (2329)" "@N (@S)" 5 9.95902 
COMMENT                                   -11.6192 0 -10 4.41284 0.99165 #246#) 
COMMENT                             #248=(CScratch 
COMMENT                                   (CWidget 4 (1 #1# 4) 1 2 0 0 #182# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 2.93254 1.9 0.082322 1) 
COMMENT                             #249=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #181# 27702560 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hae}II (3087)" "@N (@S)" 5 -5.07595 
COMMENT                                   -17.6821 0 -10 4.41284 0.99165 #248#) 
COMMENT                             #250=(CScratch 
COMMENT                                   (CWidget 5 (1 #1# 5) 1 2 0 0 #182# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 2.92388 1.9 0.082322 1) 
COMMENT                             #251=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #181# 28450664 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hae}II (3095)" "@N (@S)" 5 -5.48305 
COMMENT                                   -16.1629 0 -10 4.41284 0.99165 #250#) 
COMMENT                             #252=(CScratch 
COMMENT                                   (CWidget 6 (1 #1# 6) 1 2 0 0 #182# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 2.13581 1.9 0.082322 1) 
COMMENT                             #253=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #181# 24504096 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hae}II (3823)" "@N (@S)" 5 -12.5362 
COMMENT                                   -6.95544 0 -10 4.41284 0.99165 #252#) 
COMMENT                             #254=(CScratch 
COMMENT                                   (CWidget 7 (1 #1# 7) 1 2 0 0 #182# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 0.32259 1.9 0.082322 1) 
COMMENT                             #255=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #181# 27750512 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hae}II (5498)" "@N (@S)" 5 -6.69495 
COMMENT                                   13.0323 0 -10 4.41284 0.99165 #254#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #256=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -581 100) 
COMMENT                     (CObjectList 
COMMENT                      #257=(CLabel 
COMMENT                            (CWidget 0 (15 #18# 0) 1 2 0 0 #153# 24510568 100) 
COMMENT                            (LOGPEN 11 0 1769234804) 7564911 
COMMENT                            (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 21-OCT-2014" 
COMMENT                            "Last modified: 21-OCT-2014" 65535 -0.173539 
COMMENT                            -4.78471 0 -10 11.1809 1.06602 Nil) 
COMMENT                      #258=(CLabel (CWidget 0 (15 #19# 0) 1 2 0 0 #153# 0 100) 
COMMENT                            (LOGPEN 0 10 25124808) 0 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 8293" 
COMMENT                            "LMBP 8293" 65535 0.0743738 -2.90058 0 -10 6.84239 
COMMENT                            1.56185 Nil)) (CObjectList)) 
COMMENT               #259=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 539587699 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 24558776 
COMMENT       (CStringList) 268786312 24558812 (CObList)))
FEATURES             Location/Qualifiers
     terminator      2561..2657
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(2561..2657)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(2711..3166)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        3167..3262
                     /vntifkey="30"
                     /label=ampicillin\resistance\promoter
     RBS             3263..3297
                     /vntifkey="32"
                     /label=5'\UTR\of\amp,\incl.\RBS
     promoter        3318..3599
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      3556..3631
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             3691..4485
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4676..4745
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     rep_origin      5463..5789
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        1..568
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     CDS             613..1329
                     /vntifkey="4"
                     /label=EGFP
     CDS             1396..2340
                     /vntifkey="4"
                     /label=NFKBIA\hybrid
BASE COUNT     1388 a      1587 c      1558 g      1262 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc 
     1381 gcgggcccgg gatccatgtt ccaggcggcc gagcgccccc aggagtgggc catggagggc 
     1441 ccccgcgacg ggctgaagaa ggagcggcta ctggacgacc gccacgacag cggcctggac 
     1501 tccatgaaag acgaggagta cgagcagatg gtcaaggagc tgcaggagat ccgcctcgag 
     1561 ccgcaggagg tgccgcgcgg ctcggagccc tggaagcagc agctcaccga ggacggggac 
     1621 tcgttcctgc acttggccat catccatgaa gagaaggcac tgaccatgga agtggtccgc 
     1681 caagtgaagg gagatctggc ttttcttaac ttccagaaca acctgcagca gactccactc 
     1741 cacttggcgg tgatcaccaa ccagccagaa atcgctgagg cacttctgga agctggctgt 
     1801 gatcctgagc tccgagactt tcgaggaaat acccctctac accttgcctg tgagcagggc 
     1861 tgcctggcca gtgtgggagt cctgactcag ccccgcggga cccagcacct ccactccatt 
     1921 ctgcaggcca ccaactacaa tggccacaca tgtctgcact tagcctcgat ccatggctac 
     1981 ctgggcattg tggagctgtt ggtgtctttg ggtgctgatg tcaacgctca ggagccctgc 
     2041 aatggccgaa ccgccctgca tcttgcggtg gacctgcaga atcccgacct ggtgtcgctc 
     2101 ttgttgaagt gtggggctga tgtcaacaga gtcacctacc agggctactc cccgtaccag 
     2161 ctcacctggg gccgcccaag cactcggata cagcagcagc tgggccagct gaccctagaa 
     2221 aacctccaga tgcttccaga gagcgaggat gaggagagct atgacacgga gtcagagttc 
     2281 acagaggatg agctgcccta tgacgactgc gtgcttggag gccagcgcct gacgttatga 
     2341 gctttggaaa gtgttaaaag accatgtact tgtacatttg tacaaaatca agagttttat 
     2401 tttttaaaaa aaaaaaaaaa aaactcgagg gggggcccgg taccgcgggc ccgggatcca 
     2461 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt 
     2521 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg 
     2581 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 
     2641 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 
     2701 cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 
     2761 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 
     2821 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 
     2881 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 
     2941 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 
     3001 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 
     3061 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 
     3121 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt 
     3181 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 
     3241 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct 
     3301 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 
     3361 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 
     3421 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 
     3481 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 
     3541 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 
     3601 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc 
     3661 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 
     3721 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 
     3781 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 
     3841 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg 
     3901 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 
     3961 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 
     4021 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 
     4081 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 
     4141 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 
     4201 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 
     4261 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 
     4321 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 
     4381 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 
     4441 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt 
     4501 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg 
     4561 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 
     4621 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac 
     4681 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa 
     4741 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg 
     4801 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac 
     4861 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc 
     4921 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat 
     4981 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 
     5041 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 
     5101 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 
     5161 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 
     5221 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact 
     5281 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 
     5341 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 
     5401 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 
     5461 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 
     5521 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 
     5581 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 
     5641 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 
     5701 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 
     5761 ctgattctgt ggataaccgt attaccgcca tgcat 
//
