LOCUS       pENTR221-CeCYLD         5979 bp    DNA     circular     21-MAR-2013
DEFINITION  LMBP 8337.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|657124146|
COMMENT     VNTNAME|pENTR221-CeCYLD|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 2 1546 0 3619 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 3 2426 0 2880 0 4187 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 4292 0 5849 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 4926 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 2873 0 2920 0 4834 0 0 
COMMENT            "") #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2785 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 3091 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 3872 0 5496 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "")) 
COMMENT       (CObList 
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COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #11=(CFSignal (CObList) "attL2" 21 0 1 5972 88 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #12=(CFSignal (CObList) "rrnB T2 transcription terminator" 43 0 0 2133 
COMMENT             1 "") 
COMMENT        #13=(CFSignal (CObList) "rrnB T1 transcription terminator" 43 0 0 2292 
COMMENT             1 "") 
COMMENT        #14=(CFSignal (CObList) "T7 promoter" 30 0 1 109 125 0 (CStringList) 
COMMENT        #15=(CFSignal (CObList) "T7 primer" 27 0 1 106 125 0 (CStringList) 
COMMENT        #16=(CFSignal (CObList) "M13 reverse primer" 27 0 1 130 146 0 
COMMENT        #17=(CFSignal (CObList) "M13 (-40) forward primer" 27 0 0 2382 2398 0 
COMMENT        #18=(CFSignal (CObList) "M13 (-20) forward primer" 27 0 0 2402 2417 0 
COMMENT        #19=(CFSignal (CObList) "Kan(R)" 4 0 0 259 1068 0 (CStringList) 
COMMENT        #20=(CFSignal (CObList) "CeCYLD" 4 0 0 2537 5968 0 (CStringList) 
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COMMENT                          "Created: 03/21/13 02:29" 1) 
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COMMENT                          "Last Modified: 03/21/13 02:29" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5979 bp" 1) 
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COMMENT                          1) 
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COMMENT                          "Thaw, vortex and add BP Clonase enzyme. Incubate >= 1 hr. at 25\260." 
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COMMENT                          "Add Proteinase K for 10 min at 37\260." 1) 
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COMMENT                          1) 
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COMMENT                          "Select Entry Clones on agar plates with antibiotic kanamycin." 
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COMMENT                          "CYCGRG" "GRGCYC") 
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FEATURES             Location/Qualifiers
     misc_feature    2435..2530
                     /vntifkey="21"
                     /label=attL1
     misc_feature    complement(5972..88)
                     /vntifkey="21"
                     /label=attL2
     terminator      2133..2160
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      2292..2335
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     promoter        complement(109..125)
                     /vntifkey="30"
                     /label=T7\promoter
     primer          complement(106..125)
                     /vntifkey="27"
                     /label=T7\primer
     primer          complement(130..146)
                     /vntifkey="27"
                     /label=M13\reverse\primer
     primer          2382..2398
                     /vntifkey="27"
                     /label=M13\(-40)\forward\primer
     primer          2402..2417
                     /vntifkey="27"
                     /label=M13\(-20)\forward\primer
     CDS             259..1068
                     /vntifkey="4"
                     /label=Kan(R)
     CDS             2537..5968
                     /vntifkey="4"
                     /label=CeCYLD
BASE COUNT     1691 a      1313 c      1353 g      1622 t 
ORIGIN
        1 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc 
       61 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg 
      121 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt 
      181 tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc ttacataaac 
      241 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat 
      301 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca 
      361 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat 
      421 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg 
      481 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta 
      541 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca 
      601 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt 
      661 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg 
      721 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa 
      781 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat 
      841 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat 
      901 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc 
      961 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct 
     1021 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt 
     1081 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga 
     1141 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag 
     1201 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     1261 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     1321 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 
     1381 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     1441 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     1501 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     1561 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     1621 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     1681 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     1741 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     1801 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
     1861 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 
     1921 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 
     1981 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 
     2041 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt 
     2101 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct 
     2161 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt 
     2221 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg 
     2281 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat 
     2341 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt 
     2401 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata 
     2461 gtgacctgtt cgttgcaaca cattgatgag caatgctttt ttataatgcc aactttgtac 
     2521 aaaaaagcag gctaccatgc ccggcgaatt tgcaaagttt ccggagcaat cagctggatc 
     2581 tcatcaattt cgtaacccac tcgatgaact tacgggatca aatacaataa caaatttaac 
     2641 tgcacacgat tttgattatt ttacacagtc cgagaaaaat tcaaatttat atacaagtga 
     2701 aaatacaagc aattctaaca gattaaatct caatcaaaat ataccggtcg gcacattaat 
     2761 agatggattt gagcttatta atgaagcttc tggctccggc tttttagatg atcaattagt 
     2821 ggatgttagc gattatagcc gagataggac gacgaagctg gataggaata ggaattctcc 
     2881 cgagctaata gttgctcttt tgcaacgaaa agttcaagga attcgatttt catcgaatta 
     2941 tggaagagag gaagaaccat gtgttattga gatcccacca ggaacaatgg ttcgcgaaat 
     3001 ggcagatgat gattggaaaa tgtctgaatt aaaagaatgg ttcacaaaat caagagcttc 
     3061 ttcgcattta cgtgatggat tagcaatgcc catggaggaa ctcgactgca ctcctctgat 
     3121 ttgtgcaatg attaccagat ccgatgtaat gagaataaac caagatcaag caatacatct 
     3181 tctggctgtc agtgtagaga aacgaataga agtttatcaa aatttcgaat ggttcaattt 
     3241 cattctgaat ctgaaaattg gcgattctgt ttctgtggaa gtggacgaaa caatgcgacg 
     3301 ggttccagca aaagtgtcgt ggatcggtga gagaccggaa gcatcaggaa tatggtataa 
     3361 tgtggacttt gacgggaaca cttctcagtg gccttcttca aatcaatcat attcatcatc 
     3421 ccatgaccga ctgaatcgtc aatttgatac gaattggaat tttgaaatgt ctggatcttc 
     3481 ttcagttgca ccttccaatt cccgtctata ttattcaccc aatcaaatgc atatgccaat 
     3541 gaaaggcggt ggagtgtcag ctctatacga taatagaagg cttgtgcaat attctggaga 
     3601 tgaagaacaa tatcgaagtg caccaaaacc ggctccaaga gaacgaatta ttccagtttc 
     3661 aaggcaacaa ccagaaattg aacaacggaa tagtcgatct atgaaacctt ctgaaccgga 
     3721 ttataatact tattcgactc atccaccacg gccgccacca tcatcatcaa tgaattatcc 
     3781 atctatgtca aatacacatt cattacaacc aagtcgaagt aaaagtgttc aaacaattca 
     3841 aagaaatcaa ttcgttcgac aaaatcctgc agaaagaaat gaacgagttg aaagtgatca 
     3901 actgaacttt cgaattggtg atcaatgtat ttggaataat aatggagctg aggaaagagg 
     3961 aattattaaa tatattggtt tcttgaaagg gcacaaaaca ttatatgctg gtgtcgaatt 
     4021 taaaaacaca attggagcag gcactggtgt cttcaatcga gaacagctat tccttgctaa 
     4081 agatggtcat gctggtttcg ttgaactttc ttcactggaa tcaccatcct cttctatatc 
     4141 atccacgtct acatcatcat ctcagcatcg tcgtcgatta tcttcctcga ggagtcaaca 
     4201 gatgccagct gcatcgggaa cttccattag tgtcccggtt aatggacgtc acaatgtgaa 
     4261 tggaaagcaa gcgtcgatag aatcactttc ggatcctcca ccaccaccgt acgcaccgcc 
     4321 atctccgcca agagcatcgt ctgccgctgc ggctcaccag caaccaccgc ctttgcctcc 
     4381 gaagccatta gctagagtgg aagattttct gattgattcg tatgatattg gaagtcatgt 
     4441 tgttgtcaga catcttggag cacaacgtac aggagttgtt cgatggctcg gcgaggaaat 
     4501 tgacgacgaa ggagatcgaa ttgttcgttc agcgattgtt caactggatg aggatgttcc 
     4561 gacggcttgg cgtcgaagtc atgatgcttc ctcgtacact ggttctccag tagttggagg 
     4621 tgttcttgtt ccgattcaat ctctgaagca caatcattcg ggatcgtctt ccaatggagc 
     4681 ttctgtagtt cagacttcaa cctatccaac tagccaaacg tataataaca tcagccggcg 
     4741 aactgaagat ttcggaagta tggattcagg agtggagaag caaaaatgtg gaattgcaaa 
     4801 ggacatgcaa caattagttg gcaggcagaa aggaattcag ggttactgta actcgtgtta 
     4861 tctggatgcc acgttgtatg caatgtttgt ccaaaccact tgctttgatt ttcttcttga 
     4921 aaaatcgatc aaaggatcag aaacggctca acaatttcaa aaaattctgg cccacgagat 
     4981 cgtgttccct cttcgaaaag ttcattatgt gcgagcggat catgtcatga aacttcgaaa 
     5041 attgctcgct gagctcatgc cgcatgtaac agggcttaca aatgaagaaa aagatccaga 
     5101 agagattctt ggattcatat tctccaaagt tttccatgct gagccattca taaagttgat 
     5161 cggacaaaat cacgcaaaag attctcaata tcttgtacca attgtggtgg atgattggct 
     5221 gggaggtgca gcaacttcac agcatttgct cgaacggcat atgagatcag ctcaagtaac 
     5281 atttgcaaaa gctcctccag tattgattat gcaattacca agatatggac aacagaaagt 
     5341 gtttgataag attcttcctc tggaaacaat tgacattact ccgttcgttg ctggagcggt 
     5401 tccggcttgt tcaaaatgtc aagcatgctc agaagtctac tgcccgacgt gtttcctgac 
     5461 gagaagagtt ttctattcgg aagtgatatt ctgcagaaaa tgtttccacc atacacatct 
     5521 tttgccggaa attgaagatc acaagagtcg agatctctac ccacctggaa aaccacaaaa 
     5581 gaaaccacac tcccacaaaa tggttctctc agcggttctg tgcatagaaa catctcatta 
     5641 cgttgcttat gttcgaactt catcgaatca atgggtattc tttgattcga tggctgatcg 
     5701 agaaggacta tctgatgggt tcaatgttcc agtggtcaga gaatgtggaa atatgtccga 
     5761 ttggttatca ctgcaaggat ggaatcgttt gaaggatgct gatgagtgtg gacagattaa 
     5821 ggcaatgttc ggcaaacaat catcgttgga tcctctggta ggacgtctcc tttccgacag 
     5881 ttatatttgt ttctacgagg acgcctcacc gacatcttcg tcatcttcct cctctaccat 
     5941 cataagctcg attaaaaata tgatgaacac ccagctttc 
//
