LOCUS       pENTR-MALT1C-dC100-Y631A        3717 bp    DNA     circular     10-APR-2013
DEFINITION  LMBP 8343.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|658931295|
COMMENT     VNTDBDATE|658931295|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENTR-MALT1C-dC100-Y631A|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 2426 0 0 "") 
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COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
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COMMENT        #15=(CFSignal (CObList) "T7 primer" 27 0 1 106 125 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "M13 reverse primer" 27 0 1 130 146 0 
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COMMENT        #17=(CFSignal (CObList) "M13 (-40) forward primer" 27 0 0 2382 2398 0 
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COMMENT        #18=(CFSignal (CObList) "M13 (-20) forward primer" 27 0 0 2402 2417 0 
COMMENT             (CStringList) (CStringList "/invitrogen=\"1980000\"") 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "Kan(R)" 4 0 0 259 1068 0 (CStringList) 
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COMMENT        #20=(CFSignal (CObList) "MALT1C-dC100" 4 0 0 2534 3706 0 (CStringList) 
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COMMENT                          "Created: 04/10/13 12:28" 1) 
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COMMENT                          "If necessary, linearize attB sequence attB1-hSTAT1-attB2 at a unique REN site outside the attB region. Ethanol precipitate and resuspend." 
COMMENT                          1) 
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COMMENT                          "Thaw, vortex and add BP Clonase enzyme. Incubate >= 1 hr. at 25\260." 
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COMMENT                          "Add Proteinase K for 10 min at 37\260." 1) 
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COMMENT                          "Transform into DH5a or TOP10 E. coli or any non-F' episome bearing strain." 
COMMENT                          1) 
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COMMENT                          "Select Entry Clones on agar plates with antibiotic kanamycin." 
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FEATURES             Location/Qualifiers
     misc_feature    2435..2530
                     /vntifkey="21"
                     /label=attL1
     misc_feature    complement(3710..88)
                     /vntifkey="21"
                     /label=attL2
     terminator      2133..2160
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      2292..2335
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     promoter        complement(109..125)
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     primer          complement(106..125)
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     primer          complement(130..146)
                     /invitrogen="2010000"
                     /vntifkey="27"
                     /label=M13\reverse\primer
     primer          2382..2398
                     /invitrogen="2000000"
                     /vntifkey="27"
                     /label=M13\(-40)\forward\primer
     primer          2402..2417
                     /invitrogen="1980000"
                     /vntifkey="27"
                     /label=M13\(-20)\forward\primer
     CDS             259..1068
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     CDS             2534..3706
                     /vntifkey="4"
                     /label=MALT1C-dC100
     misc_feature    2564..2956
                     /vntifkey="21"
                     /label=CASP\p20-like
     misc_feature    2957..3220
                     /vntifkey="21"
                     /label=CASP\p10-similar\(2nd\structure)
     misc_feature    2639..2662
                     /vntifkey="21"
                     /label=nuclear\export\signal
     misc_feature    3264..3658
                     /vntifkey="21"
                     /label=gamma-adaptin-like\EAR\domain\(Ig3)
     mutation        3425..3427
                     /vntifkey="62"
                     /label=Y631A
BASE COUNT     1015 a       802 c       888 g      1012 t 
ORIGIN
        1 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc 
       61 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg 
      121 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt 
      181 tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc ttacataaac 
      241 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat 
      301 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca 
      361 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat 
      421 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg 
      481 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta 
      541 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca 
      601 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt 
      661 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg 
      721 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa 
      781 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat 
      841 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat 
      901 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc 
      961 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct 
     1021 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt 
     1081 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga 
     1141 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag 
     1201 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     1261 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     1321 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 
     1381 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     1441 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     1501 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     1561 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     1621 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     1681 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     1741 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     1801 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
     1861 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 
     1921 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 
     1981 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 
     2041 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt 
     2101 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct 
     2161 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt 
     2221 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg 
     2281 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat 
     2341 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt 
     2401 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata 
     2461 gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac 
     2521 aaaaaagcag gctacaactg accagccttt ggcgaaggac aaggttgccc ttttgatagg 
     2581 aaatatgaat taccgggagc accccaagct caaagctcct ttggtggatg tgtacgaatt 
     2641 gactaactta ctgagacagc tggacttcaa agtggtttca ctgttggatc ttactgaata 
     2701 tgagatgcgt aatgctgtgg atgagttttt actcctttta gacaagggag tatatgggtt 
     2761 attatattat gcaggacatg gttatgaaaa ttttgggaac agcttcatgg tccccgttga 
     2821 tgctccaaat ccatataggt ctgaaaattg tctgtgtgta caaaatatac tgaaattgat 
     2881 gcaagaaaaa gaaactggac ttaatgtgtt cttattggat atgtgtagga aaagaaatga 
     2941 ctacgatgat accattccaa tcttggatgc actaaaagtc accgccaata ttgtgtttgg 
     3001 atatgccacg tgtcaaggag ctgaagcttt tgaaatccag cattctggat tggcaaatgg 
     3061 aatctttatg aaatttttag aagacagatt attagaagat aagaaaatca ctgtgttact 
     3121 ggatgaagtt gcagaagata tgggtaagtg tcaccttacc aaaggcaaac aggctctaga 
     3181 gattcgaagt agtttatctg agaagagagc acttactgat ccaatacagg gaacagaata 
     3241 ttctgctgaa tctcttgtgc ggaatctaca gtgggccaag gctcatgaac ttccagaaag 
     3301 tatgtgtctt aagtttgact gtggtgttca gattcaatta ggatttgcag ctgagttttc 
     3361 caatgtcatg atcatctata caagtatagt ttacaaacca ccggagataa taatgtgtga 
     3421 tgccgcagtt actgattttc cacttgatct agatattgat ccaaaagatg caaataaagg 
     3481 cacgcctgaa gaaactggca gctacttggt atcaaaggat cttcccaagc attgcctcta 
     3541 taccagactc agttcactgc aaaaattaaa ggaacatcta gtcttcacag tatgtttatc 
     3601 atatcagtac tcaggattgg aagatactgt agaggacaag caggaagtga atgttgggaa 
     3661 acctctcatt gctaaattag acatgcatcg aggtttggga aggaagaccc agctttc 
//
