LOCUS       pVSV-N-GW               6788 bp    DNA     circular     11-JUN-2013
DEFINITION  LMBP 8370.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8370
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-JUN-2013
            
            
            
            
            
COMMENT     VNTDATE|664556811|
COMMENT     VNTNAME|pVSV-N-GW|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pVSV-N-GW" 0 6788 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "HindIII" "NcoI" "PvuI" "XhoI" "BstXI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 730 735 1 6788 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 665 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 386 0 1495 0 4329 0 5026 
COMMENT            0 0 "") #2=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 5783 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2477 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 2 2182 0 2466 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "HSV-TK polyA" 43 0 1 3839 3908 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2111..2180)") 
COMMENT        #6=(CFSignal (CObList) "SV40 ori" 33 0 1 4947 5022 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3219..3294)") 
COMMENT        #7=(CFSignal (CObList) "SV40 early promoter" 29 0 1 4979 5260 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3251..3532)") 
COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 5339 6199 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3611..4471)") 
COMMENT        #9=(CFSignal (CObList) "f1 ori" 33 0 0 6331 6786 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4603..5058") 
COMMENT        #10=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 1 4099 4887 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2371..3159)") 
COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 2795 3121 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1067..1393)") 
COMMENT        #12=(CFSignal (CObList) "T7 promoter" 30 0 0 638 654 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "638..654") 
COMMENT        #13=(CFSignal (CObList) "BGH polyA" 43 0 0 2547 2771 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "819..1043") 
COMMENT        #14=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 10 593 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "10..593") 
COMMENT        #15=(CFSignal (CObList) "attR1" 0 0 1 747 871 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 875 931 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 980 1639 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "ccdB" 4 0 0 1981 2286 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "attR2" 0 0 0 2327 2451 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #20=(CFSignal (CObList) "VSV epitope" 22 0 0 697 729 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #21=(CFSignal (CObList) "Last modified: 11-JUN-2013" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #22=(CFSignal (CObList) "LMBP 8370" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                          "DNA Plasmid 'pVSV-N-GW'" 1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 06/11/13 03:06" 1) 
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COMMENT                          "Last Modified: 06/11/13 03:06" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6788 bp" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 23-NOV-2011" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "GeneCorner Plasmid Collection" 1))) "Original Author") 
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COMMENT                                 "" 1))) "Last modified: 11-JUN-2013") 
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COMMENT                                 "" 1))) "LMBP 8370"))) "Annotations") 
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COMMENT                          "Attenuator (2 total)") 
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COMMENT                                 "chloramphenicol resistance") 
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COMMENT                                 "Tn5 neomycin resistance") 
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COMMENT                                        "   complement(3611..4471)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (4 total)") 
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COMMENT                                 "VSV epitope"))) "Misc. Marker (1 total)") 
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COMMENT                                   #70=(CLinePar 
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COMMENT                                 "hCMV-IE promoter and enhancer") 
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COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3251..3532)" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #75=(CFolderPar 
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COMMENT                                   #79=(CLinePar 
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COMMENT                                        "   638..654" 1))) "T7 promoter") 
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COMMENT                                        "Start: 875   End: 931 " 1))) 
COMMENT                                 "lacUV5 promoter"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
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COMMENT                           (CObjectList 
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COMMENT                                        "Start: 2795  End: 3121 (Complementary)" 
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COMMENT                                   #86=(CLinePar 
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COMMENT                                        "   complement(1067..1393)" 1))) 
COMMENT                                 "pMB1 ori") 
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COMMENT                                 "SV40 ori") 
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COMMENT                          "Replication Origin (3 total)") 
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COMMENT                   "Feature Map") 
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COMMENT                    "Restriction/Methylation Map") 
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FEATURES             Location/Qualifiers
     terminator      complement(3839..3908)
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     rep_origin      complement(4947..5022)
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(4979..5260)
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             complement(5339..6199)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      6331..6786
                     /vntifkey="33"
                     /label=f1\ori
     CDS             complement(4099..4887)
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     rep_origin      complement(2795..3121)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        638..654
                     /vntifkey="30"
                     /label=T7\promoter
     terminator      2547..2771
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        10..593
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     attenuator      complement(747..871)
                     /vntifkey="0"
                     /label=attR1
     promoter        875..931
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             980..1639
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             1981..2286
                     /vntifkey="4"
                     /label=ccdB
     attenuator      2327..2451
                     /vntifkey="0"
                     /label=attR2
     misc_marker     697..729
                     /vntifkey="22"
                     /label=VSV\epitope
BASE COUNT     1683 a      1685 c      1703 g      1717 t 
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg 
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc 
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga 
      661 cccaagcttg gtaccgagct cggatccgcc accatgtaca ccgacatcga gatgaaccgg 
      721 ttgggcaagg aattctgcag atatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt 
      781 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat 
      841 actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac 
      901 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgg cgagattttc 
      961 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc 
     1021 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa 
     1081 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa 
     1141 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg 
     1201 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt 
     1261 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg 
     1321 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt 
     1381 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac 
     1441 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg 
     1501 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc 
     1561 cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga 
     1621 gtggcagggc ggggcgtaaa gatctggatc cggcttacta aaagccagat aacagtatgc 
     1681 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat 
     1741 gtcaaaaaga ggtgtgctat gaagcagcgt attacagtga cagttgacag cgacagctat 
     1801 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga 
     1861 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg 
     1921 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg gactggtgaa 
     1981 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta 
     2041 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt 
     2101 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc 
     2161 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg 
     2221 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga 
     2281 atataaatgt caggctccgt tatacacagc cagtctgcag gtcgaccata gtgactggat 
     2341 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt 
     2401 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg ttgatatcca 
     2461 tcacactggc ggccgctcga gcatgcatct agagggccct attctatagt gtcacctaaa 
     2521 tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 
     2581 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 
     2641 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 
     2701 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 
     2761 gggctctatg gcttctgagg cggaaagaac cagtggcggt aatacggtta tccacagaat 
     2821 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 
     2881 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
     2941 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
     3001 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
     3061 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 
     3121 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
     3181 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
     3241 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
     3301 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 
     3361 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
     3421 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     3481 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     3541 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
     3601 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg 
     3661 cagggcctgc cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt 
     3721 ggggtgggga aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat 
     3781 cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt 
     3841 gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct 
     3901 tccgtgtttc agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc 
     3961 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga 
     4021 aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt 
     4081 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg 
     4141 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag 
     4201 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag 
     4261 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca 
     4321 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc 
     4381 gaacagttcg gctggcgcga gcccctgatg ctcttgatca tcctgatcga caagaccggc 
     4441 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt 
     4501 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc 
     4561 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc 
     4621 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag 
     4681 ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt 
     4741 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc 
     4801 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc 
     4861 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat 
     4921 ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg 
     4981 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat 
     5041 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg 
     5101 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc 
     5161 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc 
     5221 ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg 
     5281 actcttcctt tttcaataaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 
     5341 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     5401 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     5461 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
     5521 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     5581 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     5641 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     5701 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     5761 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     5821 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     5881 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     5941 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     6001 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     6061 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     6121 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     6181 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     6241 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 
     6301 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg 
     6361 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 
     6421 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 
     6481 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 
     6541 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 
     6601 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 
     6661 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 
     6721 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta 
     6781 caatttac 
//
