LOCUS       pCR3-GW-GST-C           7412 bp    DNA     circular      5-DEC-2013
DEFINITION  LMBP 8374.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8374
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 05-DEC-2013
            
COMMENT     VNTDATE|664563625|
COMMENT     VNTNAME|pCR3-GW-GST-C|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCR3-GW-GST-C" 0 7412 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "HindIII" "NcoI" "PvuI" "XhoI" "ScaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (CMolPar 0 0 1 6593 6592 1 7412 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 665 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 386 0 1470 0 4953 0 5650 
COMMENT            0 0 "") #2=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 6407 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2452 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "ScaI" "agtact" 4 0 3 1586 0 3029 0 6517 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "HSV-TK polyA" 43 0 1 4463 4532 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4463..4532)") 
COMMENT        #6=(CFSignal (CObList) "SV40 ori" 33 0 1 5571 5646 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(5571..5646)") 
COMMENT        #7=(CFSignal (CObList) "SV40 early promoter" 29 0 1 5603 5884 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(5603..5884)") 
COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 5963 6823 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(5963..6823)") 
COMMENT        #9=(CFSignal (CObList) "f1 ori" 33 0 0 6955 7410 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "6955..7410") 
COMMENT        #10=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 1 4723 5511 0 
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COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 3419 3745 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3419..3745)") 
COMMENT        #12=(CFSignal (CObList) "T7 promoter" 30 0 0 638 654 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "638..654") 
COMMENT        #13=(CFSignal (CObList) "BGH polyA" 43 0 0 3171 3395 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3171..3395") 
COMMENT        #14=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 10 593 0 
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COMMENT        #15=(CFSignal (CObList) "attR1" 0 0 1 722 846 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 854 906 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 955 1614 0 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 06/11/13 05:00" 1) 
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COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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FEATURES             Location/Qualifiers
     terminator      complement(4463..4532)
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     rep_origin      complement(5571..5646)
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(5603..5884)
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             complement(5963..6823)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      6955..7410
                     /vntifkey="33"
                     /label=f1\ori
     CDS             complement(4723..5511)
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     rep_origin      complement(3419..3745)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        638..654
                     /vntifkey="30"
                     /label=T7\promoter
     terminator      3171..3395
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        10..593
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     attenuator      complement(722..846)
                     /vntifkey="0"
                     /label=attR1
     promoter        854..906
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             955..1614
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             1956..2261
                     /vntifkey="4"
                     /label=ccdB
     attenuator      2302..2426
                     /vntifkey="0"
                     /label=attR2
     CDS             2458..3111
                     /vntifkey="4"
                     /label=GST
     promoter        complement(3129..3145)
                     /vntifkey="30"
                     /label=SP6\promoter
BASE COUNT     1870 a      1784 c      1840 g      1918 t 
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg 
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc 
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga 
      661 cccaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc 
      721 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt 
      781 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc 
      841 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat 
      901 gtgtggattt tgagttagga tccggcgaga ttttcaggag ctaaggaagc taaaatggag 
      961 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt 
     1021 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg 
     1081 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt 
     1141 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg 
     1201 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt 
     1261 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa 
     1321 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg 
     1381 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat 
     1441 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag 
     1501 gtgctgatgc cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc 
     1561 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct 
     1621 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt 
     1681 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtgt gctatgaagc 
     1741 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt 
     1801 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga 
     1861 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa 
     1921 cggctctttt gctgacgaga acagggactg gtgaaatgca gtttaaggtt tacacctata 
     1981 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg 
     2041 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg 
     2101 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg 
     2161 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg 
     2221 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tccgttatac 
     2281 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta 
     2341 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct 
     2401 cgttcagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgagctcc 
     2461 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa 
     2521 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga 
     2581 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat 
     2641 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg 
     2701 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt 
     2761 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt 
     2821 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat 
     2881 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt 
     2941 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt 
     3001 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct 
     3061 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatg atctagaggg 
     3121 ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc 
     3181 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 
     3241 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 
     3301 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 
     3361 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagtgg 
     3421 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 
     3481 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
     3541 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 
     3601 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 
     3661 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 
     3721 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
     3781 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 
     3841 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 
     3901 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 
     3961 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 
     4021 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
     4081 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 
     4141 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 
     4201 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 
     4261 tatatgagta acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg 
     4321 ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc 
     4381 cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt 
     4441 tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc 
     4501 gggttccttc cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga 
     4561 agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga 
     4621 ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt 
     4681 tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag 
     4741 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa 
     4801 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc 
     4861 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt 
     4921 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc 
     4981 gggcatgctc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttg 
     5041 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt 
     5101 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc 
     5161 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg 
     5221 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc 
     5281 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt 
     5341 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg 
     5401 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct 
     5461 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga 
     5521 tcctcatcct gtctcttgat cgatctttgc aaaagcctag gcctccaaaa aagcctcctc 
     5581 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa 
     5641 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg 
     5701 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc 
     5761 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt 
     5821 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc 
     5881 acagctggtt ctttccgcct caggactctt cctttttcaa taaatcaatc taaagtatat 
     5941 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
     6001 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
     6061 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
     6121 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
     6181 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
     6241 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 
     6301 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
     6361 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
     6421 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 
     6481 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
     6541 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 
     6601 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
     6661 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
     6721 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
     6781 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
     6841 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     6901 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc 
     6961 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 
     7021 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 
     7081 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 
     7141 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 
     7201 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 
     7261 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 
     7321 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 
     7381 attttaacaa aatattaacg cttacaattt ac 
//
