LOCUS       pLenti6(bla)-EGFP-V5        7688 bp    DNA     circular SYN  7-OCT-2010
DEFINITION  LMBP 8397.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|567455733|
COMMENT     VNTDBDATE|567455733|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLenti6(bla)-EGFP-V5|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Invitrogen|
COMMENT     VNTOAUTHORTEL|(800)-357-3114|
COMMENT     VNTOAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTOAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTOAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTOAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT         #3=(COligo "FVR1474" "CATGGTCCTGCTGGAGTTCGTG" "" 0 (CStringList) 0) 
COMMENT         #4=(COligo "MCO294" "ACGGTGGGAGGTCTATATAAGC" "" 0 (CStringList) 0) 
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COMMENT                          "Currently local object. Original author: Invitrogen" 
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COMMENT                          "Last Modified: 10/07/10 06:35" 1) 
COMMENT                     #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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FEATURES             Location/Qualifiers
     source          1..7688
                     /invitrogen="765"
                     /vntifkey="98"
     LTR             4209..4442
                     /invitrogen="1390000"
                     /vntifkey="19"
                     /label=deltaU3/3'\LTR
     CDS             5603..6463
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     promoter        5504..5602
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             3724..4122
                     /invitrogen="1050000"
                     /vntifkey="4"
                     /label=Bsd(R)
     primer          2274..2294
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     promoter        1809..2391
                     /invitrogen="2170000"
                     /vntifkey="29"
                     /label=CMV\promoter
     LTR             4208..4261
                     /invitrogen="1410000"
                     /vntifkey="19"
                     /label=deltaU3
     promoter        3656..3723
                     /invitrogen="2340000"
                     /vntifkey="30"
                     /label=EM7\promoter
     rep_origin      5017..5472
                     /invitrogen="2520000"
                     /vntifkey="33"
                     /label=f1\origin
     LTR             230..410
                     /invitrogen="1430000"
                     /vntifkey="19"
                     /label=HIV-1\5'\LTR
     LTR             4262..4442
                     /invitrogen="1430000"
                     /vntifkey="19"
                     /label=HIV-1\3'\LTR
     misc_binding    521..565
                     /invitrogen="1780000"
                     /vntifkey="20"
                     /label=HIV-1\psi\packaging\signal
     rep_origin      6608..7281
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     protein_bind    1075..1308
                     /invitrogen="2440000"
                     /vntifkey="31"
                     /label=HIV-1\Rev\response\element
     promoter        1..229
                     /invitrogen="2270000"
                     /vntifkey="29"
                     /label=RSV\promoter
     promoter        1..410
                     /invitrogen="2280000"
                     /vntifkey="29"
                     /label=RSV/5'\LTR\hybrid\promoter
     polyA_signal    4514..4644
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     primer          complement(3207..3227)
                     /invitrogen="2140000"
                     /vntifkey="27"
                     /label=V5\reverse\primer
     CDS             3198..3239
                     /invitrogen="1350000"
                     /vntifkey="4"
                     /label=V5\epitope
     splicing_signal 520..520
                     /invitrogen="123000012"
                     /vntifkey="38"
                     /label=splice\donor
     splicing_signal 1656..1656
                     /invitrogen="123000013"
                     /vntifkey="38"
                     /label=splice\acceptor
     splicing_signal 1684..1684
                     /invitrogen="123000014"
                     /vntifkey="38"
                     /label=splice\acceptor
     TATA_signal     2309..2312
                     /invitrogen="123000002"
                     /vntifkey="41"
                     /label=TATA\box
     promoter        3294..3602
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             3249..3257
                     /invitrogen="123000001"
                     /vntifkey="4"
                     /label=3\stops
     CDS             2463..3179
                     /vntifkey="4"
                     /label=EGFP
BASE COUNT     2017 a      1885 c      1959 g      1827 t 
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca 
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga 
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt 
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg 
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg 
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt 
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga 
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct 
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata 
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg 
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg 
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat 
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca 
     2341 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag 
     2401 gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga 
     2461 gtatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 
     2521 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 
     2581 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 
     2641 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 
     2701 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 
     2761 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 
     2821 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 
     2881 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 
     2941 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 
     3001 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 
     3061 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 
     3121 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg 
     3181 agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc 
     3241 gtaccggtta gtaatgagtt tggaattaat tctgtggaat gtgtgtcagt tagggtgtgg 
     3301 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 
     3361 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 
     3421 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 
     3481 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 
     3541 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 
     3601 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt 
     3661 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa 
     3721 accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca 
     3781 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac 
     3841 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc 
     3901 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc 
     3961 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat 
     4021 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg 
     4081 attcgtgaat tgctgccctc tggttatgtg tgggagggct aagcacaatt cgagctcggt 
     4141 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 
     4201 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac 
     4261 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 
     4321 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 
     4381 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 
     4441 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 
     4501 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 
     4561 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 
     4621 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg 
     4681 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 
     4741 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 
     4801 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac 
     4861 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 
     4921 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 
     4981 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 
     5041 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     5101 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     5161 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     5221 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     5281 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     5341 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 
     5401 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 
     5461 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 
     5521 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 
     5581 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 
     5641 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 
     5701 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 
     5761 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 
     5821 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 
     5881 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 
     5941 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 
     6001 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 
     6061 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 
     6121 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 
     6181 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 
     6241 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 
     6301 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 
     6361 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 
     6421 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 
     6481 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 
     6541 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 
     6601 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 
     6661 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 
     6721 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 
     6781 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 
     6841 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 
     6901 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 
     6961 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 
     7021 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 
     7081 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 
     7141 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 
     7201 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 
     7261 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 
     7321 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 
     7381 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 
     7441 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 
     7501 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 
     7561 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 
     7621 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct 
     7681 gcaagctt 
//
