ID pLenti6-eGFP-V5_puro; SV 1; circular; unknown; STD; UNC; 9548 BP. AC unknown; DT 23-JUN.-2023 DT 23-JUN.-2023 DE LMBP 8402.. OS unknown OC CC Accession number: LMBP 8402 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 23-JUN-2023 CC NGS data implemented FH Key Location/Qualifiers FH FT misc_feature 1..16 FT /label="HIV-1 cPPT" FT terminator 17..251 FT /label="RSV/HIV-1 dU3/3' LTR" FT terminator 323..397 FT /label="SV40 polyA early" FT terminator complement(323..397) FT /label="SV40 polyA late" FT rep_origin 533..608 FT /label="SV40 ori" FT promoter complement(642..658) FT /label="T7 promoter" FT rep_origin 826..1281 FT /label="f1 ori" FT CDS 1412..2272 FT /label="ampicillin resistance" FT rep_origin 2443..3034 FT /label="pMB1 ori" FT promoter 3307..3390 FT /label="lac promoter" FT promoter 3454..3470 FT /label="T3 promoter" FT promoter 3579..3907 FT /label="RSV/HIV-1 5' LTR" FT misc_feature 4018..4062 FT /label="HIV-1 psi packaging signal" FT misc_feature 4572..4805 FT /label="HIV-1 Rev response element" FT promoter 5304..5820 FT /label="hCMV-IE promoter and enhancer" FT misc_recomb complement(5937..6061) FT /label="attR1" FT promoter 6069..6121 FT /label="lacUV5 promoter" FT CDS 6170..6829 FT /label="chloramphenicol resistance" FT CDS 7171..7476 FT /label="ccdB" FT misc_recomb 7517..7641 FT /label="attR2" FT CDS 7673..8389 FT /label="EGFP" FT misc_feature 8408..8449 FT /label="V5 epitope" FT promoter 8482..8763 FT /label="SV40 early promoter" FT rep_origin 8720..8795 FT /label="SV40 ori" FT CDS 8872..9474 FT /label="puromycin resistance" SQ SEQUENCE 9548 AA; 2952434 MW; AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATCTGCTTTT 60 TGCTTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT 120 AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC 180 CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA 240 AATCTCTAGC AGTAGTAGTT CATGTCATCT TATTATTCAG TATTTATAAC TTGCAAAGAA 300 ATGAATATCA GAGAGTGAGA GGAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 360 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 420 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGCTCTAG CTATCCCGCC CCTAACTCCG 480 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 540 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 600 GGAGGCTTTT TTGGAGGCCT AGGGACGTAC CCAATTCGCC CTATAGTGAG TCGTATTACG 660 CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC 720 TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA 780 CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGACGCG CCCTGTAGCG 840 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 900 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 960 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 1020 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 1080 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 1140 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 1200 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA 1260 AAATATTAAC GCTTACAATT TAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT 1320 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA 1380 AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT 1440 TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA 1500 AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA 1560 CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT 1620 TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG 1680 TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA 1740 TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA 1800 CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT 1860 GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC 1920 CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA 1980 ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA 2040 GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC 2100 TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA 2160 TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA 2220 ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA 2280 CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT 2340 CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT 2400 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 2460 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 2520 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 2580 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 2640 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 2700 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 2760 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 2820 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 2880 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 2940 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 3000 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 3060 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT 3120 GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA 3180 GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC 3240 CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG 3300 CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA 3360 CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG 3420 AAACAGCTAT GACCATGATT ACGCCAAGCG CGCAATTAAC CCTCACTAAA GGGAACAAAA 3480 GCTGGAGCTG CAAGCTTAAT GTAGTCTTAT GCAATACTCT TGTAGTCTTG CAACATGGTA 3540 ACGATGAGTT AGCAACATGC CTTACAAGGA GAGAAAAAGC ACCGTGCATG CCGATTGGTG 3600 GAAGTAAGGT GGTACGATCG TGCCTTATTA GGAAGGCAAC AGACGGGTCT GACATGGATT 3660 GGACGAACCA CTGAATTGCC GCATTGCAGA GATATTGTAT TTAAGTGCCT AGCTCGATAC 3720 ATAAACGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG 3780 AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT 3840 CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC 3900 TCTAGCAGTG GCGCCCGAAC AGGGACTTGA AAGCGAAAGG GAAACCAGAG GAGCTCTCTC 3960 GACGCAGGAC TCGGCTTGCT GAAGCGCGCA CGGCAAGAGG CGAGGGGCGG CGACTGGTGA 4020 GTACGCCAAA AATTTTGACT AGCGGAGGCT AGAAGGAGAG AGATGGGTGC GAGAGCGTCA 4080 GTATTAAGCG GGGGAGAATT AGATCGCGAT GGGAAAAAAT TCGGTTAAGG CCAGGGGGAA 4140 AGAAAAAATA TAAATTAAAA CATATAGTAT GGGCAAGCAG GGAGCTAGAA CGATTCGCAG 4200 TTAATCCTGG CCTGTTAGAA ACATCAGAAG GCTGTAGACA AATACTGGGA CAGCTACAAC 4260 CATCCCTTCA GACAGGATCA GAAGAACTTA GATCATTATA TAATACAGTA GCAACCCTCT 4320 ATTGTGTGCA TCAAAGGATA GAGATAAAAG ACACCAAGGA AGCTTTAGAC AAGATAGAGG 4380 AAGAGCAAAA CAAAAGTAAG ACCACCGCAC AGCAAGCGGC CGCTGATCTT CAGACCTGGA 4440 GGAGGAGATA TGAGGGACAA TTGGAGAAGT GAATTATATA AATATAAAGT AGTAAAAATT 4500 GAACCATTAG GAGTAGCACC CACCAAGGCA AAGAGAAGAG TGGTGCAGAG AGAAAAAAGA 4560 GCAGTGGGAA TAGGAGCTTT GTTCCTTGGG TTCTTGGGAG CAGCAGGAAG CACTATGGGC 4620 GCAGCGTCAA TGACGCTGAC GGTACAGGCC AGACAATTAT TGTCTGGTAT AGTGCAGCAG 4680 CAGAACAATT TGCTGAGGGC TATTGAGGCG CAACAGCATC TGTTGCAACT CACAGTCTGG 4740 GGCATCAAGC AGCTCCAGGC AAGAATCCTG GCTGTGGAAA GATACCTAAA GGATCAACAG 4800 CTCCTGGGGA TTTGGGGTTG CTCTGGAAAA CTCATTTGCA CCACTGCTGT GCCTTGGAAT 4860 GCTAGTTGGA GTAATAAATC TCTGGAACAG ATTTGGAATC ACACGACCTG GATGGAGTGG 4920 GACAGAGAAA TTAACAATTA CACAAGCTTA ATACACTCCT TAATTGAAGA ATCGCAAAAC 4980 CAGCAAGAAA AGAATGAACA AGAATTATTG GAATTAGATA AATGGGCAAG TTTGTGGAAT 5040 TGGTTTAACA TAACAAATTG GCTGTGGTAT ATAAAATTAT TCATAATGAT AGTAGGAGGC 5100 TTGGTAGGTT TAAGAATAGT TTTTGCTGTA CTTTCTATAG TGAATAGAGT TAGGCAGGGA 5160 TATTCACCAT TATCGTTTCA GACCCACCTC CCAACCCCGA GGGGACCCGA CAGGCCCGAA 5220 GGAATAGAAG AAGAAGGTGG AGAGAGAGAC AGAGACAGAT CCATTCGATT AGTGAACGGA 5280 TCTCGACGGT ATCGATAAGC TTGGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG 5340 CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA 5400 GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC 5460 CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC 5520 GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG 5580 CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC 5640 AATGGGCGTG GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC 5700 AATGGGAGTT TGTTTTGGCA CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC 5760 GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT 5820 CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA CGCCATCCAC GCTGTTTTGA CCTCCATAGA 5880 AGACACCGAC TCTAGAGGAT CCACTAGTCC AGTGTGGTGG AATTCTGCAG ATATCAACAA 5940 GTTTGTACAA AAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT 6000 AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT 6060 GGCGGCCGCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATAATGTGTG 6120 GATTTTGAGT TAGGATCCGT CGAGATTTTC AGGAGCTAAG GAAGCTAAAA TGGAGAAAAA 6180 AATCACTGGA TATACCACCG TTGATATATC CCAATGGCAT CGTAAAGAAC ATTTTGAGGC 6240 ATTTCAGTCA GTTGCTCAAT GTACCTATAA CCAGACCGTT CAGCTGGATA TTACGGCCTT 6300 TTTAAAGACC GTAAAGAAAA ATAAGCACAA GTTTTATCCG GCCTTTATTC ACATTCTTGC 6360 CCGCCTGATG AATGCTCATC CGGAATTCCG TATGGCAATG AAAGACGGTG AGCTGGTGAT 6420 ATGGGATAGT GTTCACCCTT GTTACACCGT TTTCCATGAG CAAACTGAAA CGTTTTCATC 6480 GCTCTGGAGT GAATACCACG ACGATTTCCG GCAGTTTCTA CACATATATT CGCAAGATGT 6540 GGCGTGTTAC GGTGAAAACC TGGCCTATTT CCCTAAAGGG TTTATTGAGA ATATGTTTTT 6600 CGTCTCAGCC AATCCCTGGG TGAGTTTCAC CAGTTTTGAT TTAAACGTGG CCAATATGGA 6660 CAACTTCTTC GCCCCCGTTT TCACCATGGG CAAATATTAT ACGCAAGGCG ACAAGGTGCT 6720 GATGCCGCTG GCGATTCAGG TTCATCATGC CGTTTGTGAT GGCTTCCATG TCGGCAGAAT 6780 GCTTAATGAA TTACAACAGT ACTGCGATGA GTGGCAGGGC GGGGCGTAAA GATCTGGATC 6840 CGGCTTACTA AAAGCCAGAT AACAGTATGC GTATTTGCGC GCTGATTTTT GCGGTATAAG 6900 AATATATACT GATATGTATA CCCGAAGTAT GTCAAAAAGA GGTATGCTAT GAAGCAGCGT 6960 ATTACAGTGA CAGTTGACAG CGACAGCTAT CAGTTGCTCA AGGCATATAT GATGTCAATA 7020 TCTCCGGTCT GGTAAGCACA ACCATGCAGA ATGAAGCCCG TCGTCTGCGT GCCGAACGCT 7080 GGAAAGCGGA AAATCAGGAA GGGATGGCTG AGGTCGCCCG GTTTATTGAA ATGAACGGCT 7140 CTTTTGCTGA CGAGAACAGG GGCTGGTGAA ATGCAGTTTA AGGTTTACAC CTATAAAAGA 7200 GAGAGCCGTT ATCGTCTGTT TGTGGATGTA CAGAGTGATA TTATTGACAC GCCCGGGCGA 7260 CGGATGGTGA TCCCCCTGGC CAGTGCACGT CTGCTGTCAG ATAAAGTCTC CCGTGAACTT 7320 TACCCGGTGG TGCATATCGG GGATGAAAGC TGGCGCATGA TGACCACCGA TATGGCCAGT 7380 GTGCCGGTCT CCGTTATCGG GGAAGAAGTG GCTGATCTCA GCCACCGCGA AAATGACATC 7440 AAAAACGCCA TTAACCTGAT GTTCTGGGGA ATATAAATGT CAGGCTCCCT TATACACAGC 7500 CAGTCTGCAG GTCGACCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG 7560 TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC 7620 AGCTTTCTTG TACAAAGTGG TTGATATCCA GCACAGTGGC GGCCGCTCGA GTATGGTGAG 7680 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT 7740 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT 7800 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC 7860 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA 7920 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA 7980 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG 8040 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA 8100 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA 8160 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA 8220 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG 8280 CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA 8340 GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG AGGGCCCGCG 8400 GTTCGAAGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGC GTACCGGTTA 8460 GTAATGAGTT TGGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 8520 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 8580 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 8640 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 8700 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT 8760 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 8820 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGAC CATGGCCACC 8880 GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC CGTACGCACC 8940 CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC GGACCGCCAC 9000 ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC 9060 AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC 9120 GAAGCGGGGG CGGTGTTCGC CGAGATCGGC TCGCGCATGG CCGAGTTGAG CGGTTCCCGG 9180 CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG 9240 TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC 9300 GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCTGGGG TGCCCGCCTT CCTGGAGACC 9360 TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC 9420 GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGAGCTAAG 9480 CACAATTCGA GCTCGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC 9540 CACTTTTT 9548 //