LOCUS       pMG2-DYNLT1         3916 bp    DNA     circular     18-JUN-2008
DEFINITION  LMBP 8425.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|485437162|
COMMENT     VNTDBDATE|485437242|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMG2-DYNLT1|
COMMENT     VNTAUTHORNAME|Uta|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pMG2-DYNLT1" 0 3916 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
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COMMENT             0 0 1 348 1 348 5 352 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "EcoRI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "XbaI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 -65415 0 "" 0 "" "" "" "" "" "" "4063_pMG2-SVT" "" 
COMMENT             0 0 1 3568 349 3916 2056 626 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "XbaI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "EcoRI" "" 1 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 1 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 800 
COMMENT        0 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "BamHI" "XhoI") (CStringList "atg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 3916 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 547 0 3210 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "atg" "atg" 1 0 56 6 0 49 0 139 0 213 0 223 0 
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COMMENT            0 3485 0 3533 0 3590 0 3661 0 3667 0 3692 0 3745 0 3761 0 3769 0 
COMMENT            3814 0 3898 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "cat" "cat" 1 0 59 5 0 62 0 184 0 194 0 308 0 
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COMMENT            0 2739 0 2751 0 2883 0 2923 0 3315 0 3469 0 3532 0 3556 0 3581 0 
COMMENT            3631 0 3744 0 3768 0 3813 0 3897 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "taa" "taa" 1 0 51 67 0 98 0 157 0 188 0 208 0 
COMMENT            302 0 391 0 415 0 436 0 449 0 478 0 571 0 657 0 685 0 736 0 839 0 
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COMMENT            0 3167 0 3174 0 3294 0 3339 0 3612 0 3774 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "tta" "tta" 1 0 63 104 0 156 0 187 0 211 0 234 
COMMENT            0 414 0 423 0 433 0 442 0 535 0 570 0 665 0 903 0 1049 0 1094 0 1128 
COMMENT            0 1236 0 1323 0 1405 0 1427 0 1457 0 1462 0 1476 0 1519 0 1529 0 
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COMMENT            2672 0 2770 0 2774 0 3001 0 3135 0 3166 0 3179 0 3195 0 3257 0 3282 
COMMENT            0 3321 0 3427 0 3460 0 3605 0 3611 0 3780 0 3784 0 3835 0 3868 0 0 
COMMENT            "") 
COMMENT        #8=(CRSite (CStringList) "tga" "tga" 1 0 55 47 0 50 0 55 0 121 0 199 0 
COMMENT            272 0 345 0 378 0 384 0 396 0 598 0 704 0 781 0 1069 0 1167 0 1227 0 
COMMENT            1265 0 1359 0 1378 0 1422 0 1469 0 1498 0 1513 0 1537 0 1586 0 1650 
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COMMENT            2430 0 2446 0 2757 0 2804 0 2823 0 2914 0 2936 0 2974 0 3083 0 3151 
COMMENT            0 3330 0 3483 0 3534 0 3561 0 3591 0 3662 0 3711 0 3732 0 3759 0 
COMMENT            3762 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "tca" "tca" 1 0 84 4 0 107 0 166 0 322 0 462 0 
COMMENT            471 0 491 0 522 0 525 0 538 0 594 0 638 0 643 0 647 0 677 0 790 0 
COMMENT            806 0 811 0 952 0 959 0 975 0 1030 0 1345 0 1384 0 1400 0 1419 0 
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COMMENT            0 2596 0 2604 0 2668 0 2676 0 2893 0 3008 0 3059 0 3096 0 3200 0 
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COMMENT            0 3580 0 3583 0 3587 0 3625 0 3630 0 3637 0 3672 0 3819 0 3838 0 
COMMENT            3903 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "tag" "tag" 1 0 41 79 0 142 0 145 0 350 0 457 
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COMMENT             3264 0 3428 0 3461 0 3464 0 3473 0 3606 0 3722 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "cta" "cta" 1 0 41 14 0 76 0 268 0 340 0 349 0 
COMMENT             501 0 836 0 1060 0 1155 0 1179 0 1183 0 1189 0 1194 0 1368 0 1447 0 
COMMENT             1486 0 1545 0 1562 0 1608 0 1761 0 1782 0 1833 0 2697 0 2718 0 2760 
COMMENT             0 2808 0 2844 0 2909 0 2991 0 3049 0 3053 0 3102 0 3263 0 3293 0 
COMMENT             3366 0 3413 0 3454 0 3773 0 3810 0 3864 0 3894 0 0 "")) 
COMMENT       (CObList 
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COMMENT             (CStringList "/gene=\"SRa prom.\"" 
COMMENT              "/product=\"SV40 ori+Early prom+enhancer repeats + HTLV1-LTR\"") 1 
COMMENT             1 1 1 "4226..4822") 
COMMENT        #13=(CFSignal (CObList) "Amp" 4 0 1 1519 2379 1 (CStringList) 
COMMENT             (CStringList "/gene=\"Amp\"") 1 1 1 1 "complement (3226..4086)") 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA" 4 0 0 416 546 1 (CStringList) 
COMMENT             (CStringList "/gene=\"SV40 polyA\"") 1 1 1 1 "2123..2253") 
COMMENT        #15=(CFSignal (CObList) "gp130" 4 0 0 3349 3906 1 (CStringList) 
COMMENT             (CStringList "/gene=\"gp130\"" 
COMMENT              "/product=\"intracellular fragment of gp130 (3' end contains a duplicated region)\"") 
COMMENT             1 1 1 1 "59..616") 
COMMENT        #16=(CFSignal (CObList) "FLAG" 4 0 0 3316 3342 1 (CStringList) 
COMMENT             (CStringList "/gene=\"FLAG\"" "/product=\"FLAG tag\"") 1 1 1 1 
COMMENT             "26..52") 
COMMENT        #17=(CFSignal (CObList) "DYNLT" 4 0 0 6 347 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList #18=(CORF "atg" "tga" 1 0 3316 344 1869182051 0 0 0 30) 
COMMENT        #19=(CORF "atg" "taa" 2 1 1522 2379 1699881070 0 0 0 30)) 
COMMENT       (CTextView 0 
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COMMENT                    2) 5 "" 0 4) 
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COMMENT                          "DNA 'pMG2-DYNLT1'" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: Uta. Original author: Uta" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 06/18/08 11:39" 1) 
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COMMENT                          "Last Modified: 06/18/08 11:40" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 3916 bp" 1) 
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COMMENT                          "#1: FRAGMENT of <a href=\"vnti:dnarna/PCR%20for%20DYNLT1?sel=5-352\">PCR for DYNLT1</a> ") 
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COMMENT                                 "original length: 3568" 1) 
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COMMENT                                 "molecule position: from 349 to 3916" 1) 
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COMMENT                                        "  EcoRI site #1" 1))) "Right Terminus"))) 
COMMENT                          "#2: FRAGMENT of <a href=\"vnti:dnarna/4063_pMG2-SVT?sel=2056-626\">4063_pMG2-SVT</a> "))) 
COMMENT                   "Component Fragments") 
COMMENT              #54=(CFolderPar 
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COMMENT                                        "Start: 6     End: 347 " 1))) "DYNLT") 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2123..2253" 1) 
COMMENT                                   #62=(CLinePar 
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COMMENT                                        "Qualifiers:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /gene=\"SV40 polyA\"" 1))) 
COMMENT                                 "SV40 polyA") 
COMMENT                            #64=(CFolderPar 
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COMMENT                                  (CParagraph 1519 (3 #13# 0) 1 2 2 0 327) 
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COMMENT                                   #65=(CLinePar 
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COMMENT                                        "Start: 1519  End: 2379 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #66=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #67=(CLinePar 
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COMMENT                   "Feature Map") 
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COMMENT                                        " N1: 547 " 1) 
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COMMENT                                        " N2: 3210 " 1))))) "BamHI: 2 sites") 1 
COMMENT                          5 "GGATCC" "CCTAGG") 
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COMMENT                                                 "Region:  3316 - 344" 1))) 
COMMENT                                         "3316(d1) (315 codons)"))) 
COMMENT                                 "Reading Frame 1 (1 ORF)") 
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COMMENT                          "Direct(Forward) strand") 
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COMMENT                           "Complementary(Reverse) strand"))) 
COMMENT                   "Open Reading Frames")))) 
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FEATURES             Location/Qualifiers
     CDS             2519..3115
                     /gene="SRa prom."
                     /product="SV40 ori+Early prom+enhancer repeats + HTLV1-LTR"
                     /vntifkey="4"
                     /label=SRa\prom.
     CDS             complement(1519..2379)
                     /gene="Amp"
                     /vntifkey="4"
                     /label=Amp
     CDS             416..546
                     /gene="SV40 polyA"
                     /vntifkey="4"
                     /label=SV40\polyA
     CDS             3349..3906
                     /gene="gp130"
                     /product="intracellular fragment of gp130 (3' end contains a duplicated region)"
                     /vntifkey="4"
                     /label=gp130
     CDS             3316..3342
                     /gene="FLAG"
                     /product="FLAG tag"
                     /vntifkey="4"
                     /label=FLAG
     CDS             6..347
                     /vntifkey="4"
                     /label=DYNLT
BASE COUNT     1007 a       985 c       926 g       998 t 
ORIGIN
        1 aattcatgga agactaccag gctgcggagg agactgcttt tgttgttgat gaagtgagca 
       61 acattgtaaa agaggctata gaaagcgcaa ttggtggtaa cgcttatcaa cacagcaaag 
      121 tgaaccagtg gaccacaaat gtagtagaac aaactttaag ccaactcacc aagctgggaa 
      181 aaccatttaa atacatcgtg acctgtgtaa ttatgcagaa gaatggagct ggattacaca 
      241 cagcaagttc ctgcttctgg gacagctcta ctgacgggag ctgcactgtg cgatgggaga 
      301 ataagaccat gtactgcatc gtcagtgcct tcggactgtc tatttgatct agagaaaaaa 
      361 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt 
      421 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
      481 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 
      541 tgtctggatc cccgggtacc gagctcgaat taattcctct tccgcttcct cgctcactga 
      601 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 
      661 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 
      721 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 
      781 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 
      841 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 
      901 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc 
      961 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 
     1021 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 
     1081 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 
     1141 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 
     1201 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 
     1261 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 
     1321 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 
     1381 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 
     1441 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 
     1501 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 
     1561 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 
     1621 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 
     1681 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 
     1741 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 
     1801 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 
     1861 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 
     1921 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 
     1981 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 
     2041 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 
     2101 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 
     2161 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 
     2221 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 
     2281 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 
     2341 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 
     2401 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 
     2461 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg caagcttggc 
     2521 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 
     2581 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 
     2641 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 
     2701 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 
     2761 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 
     2821 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcctccga tcgaggggct 
     2881 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 
     2941 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 
     3001 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 
     3061 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 
     3121 tctgttctgc gccgttacag atccaagctc tgaaaaacca gaaagttaac tggtaagttt 
     3181 agtctttttg tcttttattt caggtcccgg atccggtggt gcaaatcaaa gaactgctcc 
     3241 tcagtggatg ttgcctttac ttctaggcct gtacggaagt gttacttctg ctctaaaagc 
     3301 tgcgggcccg ccaccatgga ttacaaggat gacgacgata agatctcgac cgtggtacac 
     3361 agtggctaca gacaccaagt tccgtcagtc caagtcttct caagatccga gtctacccag 
     3421 cccttgttag attcagagga gcggccagaa gatctacaat tagtagatca tgtagatggc 
     3481 ggtgatggta ttttgcccag gcaacagtac ttcaaacaga actgcagtca gcatgaatcc 
     3541 agtccagata tttcacattt tgaaaggtca aagcaagttt catcagtcaa tgaggaagat 
     3601 tttgttagac ttaaacagca gatttcagat catatttcac aatcctgtgg atctgggcaa 
     3661 atgaaaatgt ttcaggaagt ttctgcagca gatgcttttg gtccaggtac tgagggacaa 
     3721 gtagaaagat ttgaaacagt tggcatggag gctgcgactg atgaaggcat gcctaaaagt 
     3781 tacttaccac agactgtacg gcaaggcggc tacatgcctc agggcggttc ggaattatca 
     3841 acaagtttgt acaaaaaagc aggctactta ccacagactg tacggcaagg cggctacatg 
     3901 cctcagggcg gttcgg 
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