LOCUS       pMG8                    4817 bp    DNA     circular      10-JUN-2013
DEFINITION  LMBP 8459.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8459
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 10-JUN-2013
            
COMMENT     VNTDATE|663938281|
COMMENT     VNTNAME|pMG8|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        (CStringList "AatII" "Acc65I" "BsaI" "EcoRI" "HaeII" "HincII" "HindIII" 
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COMMENT        #2=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 1 1729 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4814 0 0 "") 
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COMMENT            0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HincII" "gtyrac" 4 0 2 272 0 3871 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 3869 0 0 "") 
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COMMENT                          "Last Modified: 06/10/13 01:48" 1) 
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COMMENT       (CStringList) 1094795585 1195852099 
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COMMENT        #217=(CSampFrag "pMG8" 3599 272 "HincII" 3871 "HincII" (LOGPEN 0 0 0) 
COMMENT              1.40464e-316 8.72565e-301) 
COMMENT        #218=(CSampFrag "pMG8" 1218 3871 "HincII" 272 "HincII" (LOGPEN 0 0 0) 
COMMENT              1.6976e-313 1.09369e-300))))
FEATURES             Location/Qualifiers
     misc_feature    complement(142..798)
                     /vntifkey="21"
                     /label=5'\UTR\of\amyE
     CDS             1007..1867
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      2408..2810
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        2902..2985
                     /vntifkey="30"
                     /label=lac\promoter
     misc_feature    complement(3068..3872)
                     /vntifkey="21"
                     /label=3'\UTR\of\amyE
     CDS             complement(3965..4732)
                     /vntifkey="4"
                     /label=spectinomycin\resistance\(aad9)
BASE COUNT     1262 a      1083 c      1011 g      1461 t 
ORIGIN
        1 cgagctcggt acccggggat cctctagagt cctgagcgcc ggtcgctacc attaccagtt 
       61 ggtctggtgt caaaaataat aataaccggg caggccatgt ctgcccgtat ttcgcgtaag 
      121 gaaatccatt atgtactatt taattctgcg tgacatccca tcgatcagac cagtttttaa 
      181 tttgtgtgtt tccatgtgtc cagtttggaa tactcttaac ctcattggaa atcgcggcat 
      241 aatcactggt ggtatgattg atgaccgcgt caacaatgac ctttatgcca tattcttcag 
      301 cggctgcaca catttcttta aattcttgtt cagtacctaa gtaacggttg ccaatttgat 
      361 acgatgtcgg ctgatacagc cagtaccagt tcgacatgct tttatctcct tgattccctt 
      421 cctttacttg gttaatcgga gatgtctgaa tggctgtata tcctgcatca tgaatatcct 
      481 tcatattgtg ttttaacgta ttgaacgacc aattccatgc atgaagaatg gttccgcttt 
      541 tgatcgacgg tgctgtaagc tcattcgatt tgttcgccgt ttcagcactc gcagccgccg 
      601 gtcctgccag aaccaaatga aacagcaata aaaatccagc gaataacggc agtaaagagg 
      661 ttttgaatcg ttttgcaaac attcttgaca ctccttattt gattttttga agacttactt 
      721 cggagtcaaa aatccctctt acttcattct tccgcttcct cctttcaaac cgatgtgaag 
      781 actggagaat tttgttaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata 
      841 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 
      901 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 
      961 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 
     1021 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 
     1081 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 
     1141 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 
     1201 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 
     1261 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 
     1321 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 
     1381 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 
     1441 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 
     1501 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 
     1561 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 
     1621 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 
     1681 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 
     1741 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 
     1801 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 
     1861 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 
     1921 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 
     1981 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 
     2041 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 
     2101 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 
     2161 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 
     2221 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 
     2281 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 
     2341 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 
     2401 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 
     2461 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 
     2521 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 
     2581 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 
     2641 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 
     2701 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 
     2761 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 
     2821 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 
     2881 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 
     2941 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 
     3001 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctaatcg gcggccgcat 
     3061 cgatgcaggc tcccatcatg tttatagata taagcattta cctggctcca atgattcgga 
     3121 ttttgatagc cgatggtttt ggccgacgct ggatctcttt taacaaaact gtatttctcg 
     3181 gtcctcgtta caccatcact gttcgttcct tttaacatga tggtgtatgt tttgccaaat 
     3241 ggatctcctt ttccgattgt gaattgatct ccatccttaa acgccgtctc tggtccatta 
     3301 ttgatttgat aaacggcttt tgttgtattc gcatctgcac gcaaggtaat cgtcagttga 
     3361 tcattgaaag aatgtgttac acctgttttg taattctcaa ggaaaacatg aggcgctttt 
     3421 gcaatatcat caggataaag cacagctaca gacctggcat tgatcgtgcc tgtcagttta 
     3481 ccatcgttca cttgaaatga acccgctcca gctttattgt catacctgcc atcaggcaat 
     3541 tttgttgccg tattgataga gacagaggat gaacctgcat ttgccagcac aacgccatgt 
     3601 gagccgcgct gattcataaa tatctggttg tttccattcg ggttcgagag ttcctcaggc 
     3661 tgtccagcca tcacattgtg aaatctattg accgcagtga tagcctgatc ttcaaataaa 
     3721 gcactcccgc gatcgcctat ttggcttttc cccgggaacc tcacaccatt tccgcctccc 
     3781 tcaggtctgg aaaagaaaag aggcgtactg cctgaacgag aagctatcac cgcccagcct 
     3841 aaacggatat catcatcgct catccatgtc gactctgatt accaattaga atgaatattt 
     3901 cccaaatatt aaataataaa acaaaaaaat tgaaaaaagt gtttccacca ttttttcaat 
     3961 ttttttataa tttttttaat ctgttattta aatagtttat agttaaattt acattttcat 
     4021 tagtccattc aatattctct ccaagataac tacgaactgc taacaaaatt ctctccctat 
     4081 gttctaatgg agaagattca gccactgcat ttcccgcaat atcttttggt atgattttac 
     4141 ccgtgtccat agttaaaatc atacggcata aagttaatat agagttggtt tcatcatcct 
     4201 gataattatc tattaattcc tctgacgaat ccataatggc tcttctcaca tcagaaaatg 
     4261 gaatatcagg tagtaattcc tctaagtcat aatttccgta tattctttta ttttttcgtt 
     4321 ttgcttggta aagcattatg gttaaatctg aatttaattc cttctgagga atgtatcctt 
     4381 gttcataaag ctcttgtaac cattctccat aaataaattc ttgtttggga ggatgattcc 
     4441 acggtaccat ttcttgctga ataataattg ttaattcaat atatcgtaag ttgcttttat 
     4501 ctcctatttt ttttgaaata ggtctaattt tttgtataag tatttcttta ctttgatctg 
     4561 tcaatggttc agatacgacg actaaaaagt caagatcact atttggtttt agtccactct 
     4621 caactcctga tccaaacatg taagtaccaa taaggttatt ttttaaatgt ttccgaagta 
     4681 tttttttcac tttattaatt tgttcgtatg tattcaaata tatcctcctc actattttga 
     4741 ttagtaccta ttttatatcc atagttgtta attaaataaa cccattatat cgggtttttg 
     4801 aggggatttc aagaatt 
//
