LOCUS       pMG9                    4846 bp    DNA     circular      7-JUN-2013
DEFINITION  LMBP 8460.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8460
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 7-JUN-2013
            
            
COMMENT     VNTDATE|663938371|
COMMENT     VNTNAME|pMG9|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pMG9" 0 4846 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 150 0 
COMMENT        1 0 (CHomObj 1 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) (CWordArray) 
COMMENT        (CStringList "AatII" "Acc65I" "BsaI" "EcoRI" "HaeII" "HincII" "HindIII" 
COMMENT         "NotI" "PstI" "SalI" "XbaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4846 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 881 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 14 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 1 1734 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 5 45 0 2448 0 2818 0 3422 
COMMENT            0 4167 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HincII" "gtyrac" 4 0 4 277 0 3876 0 4083 0 
COMMENT            4556 0 0 "") #6=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 3058 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "PstI" "ctgcag" 6 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 3874 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 29 0 0 "")) 
COMMENT       (CObList 
COMMENT        #11=(CFSignal (CObList) "5' UTR of amyE" 21 0 1 147 803 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #12=(CFSignal (CObList) "3' UTR of amyE" 21 0 1 3073 3877 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #13=(CFSignal (CObList) "lac promoter" 30 0 0 2907 2990 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 0 2413 2815 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "ampicillin resistance" 4 0 0 1012 1872 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "kanamycin resistance" 4 0 1 3967 4737 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #17=(CFSignal (CObList) "Last modified: 7-JUN-2013" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #18=(CFSignal (CObList) "LMBP 8460" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #19=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #20=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pMG9" 2) 5 "" 0 4) 
COMMENT              #21=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pMG9'" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 06/04/13 11:19" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 06/07/13 09:45" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4846 bp" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #29=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 4-JUN-2013" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #32=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #33=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #34=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #35=(CLinePar (CParagraph 0 (15 #17# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 7-JUN-2013") 
COMMENT                     #36=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #37=(CLinePar (CParagraph 0 (15 #18# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 8460"))) "Annotations") 
COMMENT              #38=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #39=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #40=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1012 (3 #15# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #41=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1012  End: 1872" 1))) 
COMMENT                                 "ampicillin resistance") 
COMMENT                            #42=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3967 (3 #16# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3967  End: 4737 (Complementary)" 
COMMENT                                        1))) "kanamycin resistance"))) 
COMMENT                          "CDS (2 total)") 
COMMENT                     #44=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #45=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 147 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 147   End: 803  (Complementary)" 
COMMENT                                        1))) "5' UTR of amyE") 
COMMENT                            #47=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3073 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3073  End: 3877 (Complementary)" 
COMMENT                                        1))) "3' UTR of amyE"))) 
COMMENT                          "Misc. Feature (2 total)") 
COMMENT                     #49=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #50=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2907 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2907  End: 2990" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #52=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #53=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2413 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2413  End: 2815" 1))) "pMB1 ori"))) 
COMMENT                          "Replication Origin (1 total)"))) "Feature Map") 
COMMENT              #55=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #56=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" 
COMMENT                          "TTCGAA") 
COMMENT                     #57=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "PstI: 0 sites") 5 1 "CTGCAG" 
COMMENT                          "GACGTC") 
COMMENT                     #58=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #59=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 881 " 1))))) "AatII: 1 site") 5 1 
COMMENT                          "GACGTC" "CTGCAG") 
COMMENT                     #61=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #62=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 14 " 1))))) "Acc65I: 1 site") 1 5 
COMMENT                          "GGTACC" "CCATGG") 
COMMENT                     #64=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #65=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 1734 " 1))))) "BsaI: 1 site") 7 11 
COMMENT                          "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
COMMENT                     #67=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #68=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 2 " 1))))) "EcoRI: 1 site") 1 5 
COMMENT                          "GAATTC" "CTTAAG") 
COMMENT                     #70=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #71=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #7# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 3058 " 1))))) "NotI: 1 site") 2 6 
COMMENT                          "GCGGCCGC" "CGCCGGCG") 
COMMENT                     #73=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #74=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #9# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #9# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 3874 " 1))))) "SalI: 1 site") 1 5 
COMMENT                          "GTCGAC" "CAGCTG") 
COMMENT                     #76=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #77=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #10# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #10# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 29 " 1))))) "XbaI: 1 site") 1 5 
COMMENT                          "TCTAGA" "AGATCT") 
COMMENT                     #79=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #80=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 277 " 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #5# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 3876 " 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #5# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 4083 " 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #5# 4) 1 2 2 0 191) 
COMMENT                                        " N4: 4556 " 1))))) "HincII: 4 sites") 3 
COMMENT                          3 "GTYRAC" "CARYTG") 
COMMENT                     #85=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 5 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #86=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 45 " 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 2448 " 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 2818 " 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 4) 1 2 2 0 191) 
COMMENT                                        " N4: 3422 " 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 5) 1 2 2 0 191) 
COMMENT                                        " N5: 4167 " 1))))) "HaeII: 5 sites") 5 
COMMENT                          1 "RGCGCY" "YCGCGR"))) "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #92=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111 
COMMENT               0 1 5 "@N (@S)" 0) 
COMMENT          #93=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT               "@N" 0) 
COMMENT          #94=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT               "@L bp" 0) 
COMMENT          #95=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #96=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT               0 0) 
COMMENT          #97=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT               (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #98=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT               0 0 0) 
COMMENT          #99=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #100=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #101=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #102=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #103=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #104=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #105=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #106=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #107=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #108=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #109=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #110=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) #111=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #112=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #113=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #114=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #115=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #116=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #117=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #118=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #119=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #120=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #121=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #122=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #123=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #124=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #125=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #126=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #127=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #128=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #129=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #130=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #131=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #132=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #133=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #134=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #135=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #136=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #137=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #138=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #139=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #140=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #141=(CWidgetStyle "Label 27" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #142=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #143=(CWidgetStyle "Label 28" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #144=(CWidgetStyle "Label 29" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #145=(CWidgetStyle "Mutation Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #146=(CWidgetStyle "Label 30" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #147=(CWidgetStyle "MSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #148=(CWidgetStyle "Label 34" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #149=(CWidgetStyle "Label 45" 1 (LOGPEN 0 0 3381657) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #150=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 39321) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #151=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 16724736) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 20" 1 "Label 21") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 21") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 30") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 
COMMENT         -3261) 1 1 0 1 1 
COMMENT        (mapper: 21.5907 -18.0868 40.3368 40.3368 0.01 10 -1 4846 4846 1 0 0) 
COMMENT        #152=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1701670255 100) 
COMMENT              (CObjectList 
COMMENT               #153=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -602 100) 
COMMENT                     (CObjectList 
COMMENT                      #154=(CAxis 
COMMENT                            (CWideLine (CWidget 0 (0 0) 1 2 0 0 #98# 29267740 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27548 
COMMENT                             6.27348 1 0.0214037) 0.129451) 
COMMENT                      #155=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #95# 4 100) 
COMMENT                            (LOGPEN 808736339 3420416 17564099) 2082602612 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "pMG9" "@N" 1 0 0.871165 0 -10 3.6939 
COMMENT                            1.56185 Nil) 
COMMENT                      #156=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #94# 6 100) 
COMMENT                            (LOGPEN 1414415696 536890959 17563819) 25118856 
COMMENT                            (LOGFONT 73 27 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "4846 bp" "@L bp" 16 
COMMENT                            0 -0.871165 0 -10.8 4.78471 1.80976 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #157=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -813 100) 
COMMENT                     (CObjectList 
COMMENT                      #158=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -461 100) 
COMMENT                            (CObjectList 
COMMENT                             #159=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #96# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    3.84987 4.96444 1 0.082322) 0.8 1.8 0) 
COMMENT                             #160=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "ampicillin resistance" "@N" 1 16.942 
COMMENT                                   -4.97214 0 -10 8.03237 1.16519 #159#) 
COMMENT                             #161=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #96# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    0.141101 1.13917 1 0.082322) 0.8 1.8 1) 
COMMENT                             #162=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "kanamycin resistance" "@N" 1 -13.6278 
COMMENT                                   12.4303 0 -10 8.77611 1.16519 #161#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #163=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -742 100) 
COMMENT                            (CObjectList 
COMMENT                             #164=(CLine 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #99# 730 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 5.2337 
COMMENT                                   6.08419) 
COMMENT                             #165=(CLabel (CWidget 0 (0 0) 1 2 0 0 #137# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "5' UTR of amyE" 
COMMENT                                   "@N" 1 10.4534 10.929 0 -10 6.12344 1.16519 
COMMENT                                   #164#) 
COMMENT                             #166=(CLine 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #99# 751 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 1.25438 
COMMENT                                   2.29646) 
COMMENT                             #167=(CLabel (CWidget 0 (0 0) 1 2 0 0 #137# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "3' UTR of amyE" 
COMMENT                                   "@N" 1 -16.798 -2.65961 0 -10 6.12344 1.16519 
COMMENT                                   #166#)) (CObjectList)) 
COMMENT                      #168=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -321 100) 
COMMENT                            (CObjectList 
COMMENT                             #169=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #138# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    2.40261 2.51135 1 0.082322) 0.8 1.8 0) 
COMMENT                             #170=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lac promoter" 
COMMENT                                   "@N" 1 -10.1628 -10.2735 0 -10 5.05742 
COMMENT                                   1.16519 #169#)) (CObjectList)) 
COMMENT                      #171=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -813 100) 
COMMENT                            (CObjectList 
COMMENT                             #172=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #108# 326 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    2.62915 3.15083 1 0.082322) 0.8 1.8 0) 
COMMENT                             #173=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 -5.15474 -13.6094 0 -10 3.56994 1.16519 
COMMENT                                   #172#)) (CObjectList))) (CObjectList)) 
COMMENT               #174=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -250 100) 
COMMENT                     (CObjectList 
COMMENT                      #175=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -180 100) 
COMMENT                            (CObjectList 
COMMENT                             #176=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 5.13402 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #177=(CLabel (CWidget 0 (0 0) 1 2 0 0 #148# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Aat}II (881)" "@N (@S)" 5 15.8816 
COMMENT                                   6.45961 0 -10 3.84264 0.99165 #176#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #178=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -672 100) 
COMMENT                            (CObjectList 
COMMENT                             #179=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 6.25636 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #180=(CLabel (CWidget 0 (0 0) 1 2 0 0 #148# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Acc}65I (14)" "@N (@S)" 5 2.74315 
COMMENT                                   15.8406 0 -10 4.01618 0.99165 #179#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #181=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -110 100) 
COMMENT                            (CObjectList 
COMMENT                             #182=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 4.0298 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #183=(CLabel (CWidget 0 (0 0) 1 2 0 0 #148# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Bsa}I (1734)" "@N (@S)" 5 12.4746 
COMMENT                                   -8.87825 0 -10 4.09056 0.99165 #182#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #184=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -461 100) 
COMMENT                            (CObjectList 
COMMENT                             #185=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 6.27189 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #186=(CLabel (CWidget 0 (0 0) 1 2 0 0 #148# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Eco}RI (2)" "@N (@S)" 5 -1.6891 15.8228 
COMMENT                                   0 -10 3.42119 0.99165 #185#)) (CObjectList)) 
COMMENT                      #187=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #7# 0) 1 2 0 0 Nil -602 100) 
COMMENT                            (CObjectList 
COMMENT                             #188=(CScratch 
COMMENT                                   (CWidget 1 (1 #7# 1) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 2.31587 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #189=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #148# 544 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Not}I (3058)" "@N (@S)" 5 -11.0267 
COMMENT                                   -8.95262 0 -10 4.09056 0.99165 #188#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #190=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #9# 0) 1 2 0 0 Nil -1023 100) 
COMMENT                            (CObjectList 
COMMENT                             #191=(CScratch 
COMMENT                                   (CWidget 1 (1 #9# 1) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 1.25956 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #192=(CLabel (CWidget 0 (0 0) 1 2 0 0 #148# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Sal}I (3874)" "@N (@S)" 5 -16.5821 
COMMENT                                   4.71728 0 -10 3.91702 0.99165 #191#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #193=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #10# 0) 1 2 0 0 Nil -675 100) 
COMMENT                            (CObjectList 
COMMENT                             #194=(CScratch 
COMMENT                                   (CWidget 1 (1 #10# 1) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 6.23694 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #195=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #148# 14746112 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Xba}I (29)" "@N (@S)" 5 2.71486 14.6685 
COMMENT                                   0 -10 3.32203 0.99165 #194#)) (CObjectList)) 
COMMENT                      #196=(CGroupWidget 
COMMENT                            (CWidget 4 (10 #5# 0) 1 2 0 0 Nil -1094 100) 
COMMENT                            (CObjectList 
COMMENT                             #197=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 5.9159 1.9 0.082322 1) 
COMMENT                             #198=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #149# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (277)" "@N (@S)" 5 7.08558 
COMMENT                                   12.5721 0 -10 4.23931 0.99165 #197#) 
COMMENT                             #199=(CScratch 
COMMENT                                   (CWidget 2 (1 #5# 2) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 1.25697 1.9 0.082322 1) 
COMMENT                             #200=(CLabel (CWidget 0 (0 0) 1 2 0 0 #149# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (3876)" "@N (@S)" 5 -16.6657 
COMMENT                                   5.96379 0 -10 4.66076 0.99165 #199#) 
COMMENT                             #201=(CScratch 
COMMENT                                   (CWidget 3 (1 #5# 3) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 0.989004 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #202=(CLabel (CWidget 0 (0 0) 1 2 0 0 #149# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (4083)" "@N (@S)" 5 -14.3087 
COMMENT                                   8.44985 0 -10 4.66076 0.99165 #201#) 
COMMENT                             #203=(CScratch 
COMMENT                                   (CWidget 4 (1 #5# 4) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 0.376702 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #204=(CLabel (CWidget 0 (0 0) 1 2 0 0 #149# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (4556)" "@N (@S)" 5 -7.55194 
COMMENT                                   13.9248 0 -10 4.66076 0.99165 #203#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #205=(CGroupWidget 
COMMENT                            (CWidget 5 (10 #4# 0) 1 2 0 0 Nil -532 100) 
COMMENT                            (CObjectList 
COMMENT                             #206=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 6.21623 1.9 0.082322 1) 
COMMENT                             #207=(CLabel (CWidget 0 (0 0) 1 2 0 0 #149# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hae}II (45)" "@N (@S)" 5 3.39067 
COMMENT                                   13.6699 0 -10 3.56994 0.99165 #206#) 
COMMENT                             #208=(CScratch 
COMMENT                                   (CWidget 2 (1 #4# 2) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 3.10552 1.9 0.082322 1) 
COMMENT                             #209=(CLabel (CWidget 0 (0 0) 1 2 0 0 #149# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hae}II (2448)" "@N (@S)" 5 -2.77451 
COMMENT                                   -15.1038 0 -10 4.41284 0.99165 #208#) 
COMMENT                             #210=(CScratch 
COMMENT                                   (CWidget 3 (1 #4# 3) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 2.62656 1.9 0.082322 1) 
COMMENT                             #211=(CLabel (CWidget 0 (0 0) 1 2 0 0 #149# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hae}II (2818)" "@N (@S)" 5 -8.41251 
COMMENT                                   -11.8423 0 -10 4.41284 0.99165 #210#) 
COMMENT                             #212=(CScratch 
COMMENT                                   (CWidget 4 (1 #4# 4) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 1.84467 1.9 0.082322 1) 
COMMENT                             #213=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #149# 1128482644 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hae}II (3422)" "@N (@S)" 5 -16.4755 
COMMENT                                   -4.72422 0 -10 4.41284 0.99165 #212#) 
COMMENT                             #214=(CScratch 
COMMENT                                   (CWidget 5 (1 #4# 5) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 0.880265 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #215=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #149# 1094796097 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hae}II (4167)" "@N (@S)" 5 -13.7672 
COMMENT                                   10.1922 0 -10 4.41284 0.99165 #214#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #216=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -535 100) 
COMMENT                     (CObjectList 
COMMENT                      #217=(CLabel 
COMMENT                            (CWidget 0 (15 #17# 0) 1 2 0 0 #121# 1096045635 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 7-JUN-2013" 
COMMENT                            "Last modified: 7-JUN-2013" 65535 0.0743738 -4.48722 
COMMENT                            0 -10 10.4371 1.06602 Nil) 
COMMENT                      #218=(CLabel (CWidget 0 (15 #18# 0) 1 2 0 0 #121# 0 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 8460" 
COMMENT                            "LMBP 8460" 65535 0 -2.97495 0 -10 6.84239 1.56185 
COMMENT                            Nil)) (CObjectList)) 
COMMENT               #219=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -253 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 1196705607 
COMMENT       (CStringList) 1094795585 1195852099 (CObList)))
FEATURES             Location/Qualifiers
     misc_feature    complement(147..803)
                     /vntifkey="21"
                     /label=5'\UTR\of\amyE
     misc_feature    complement(3073..3877)
                     /vntifkey="21"
                     /label=3'\UTR\of\amyE
     promoter        2907..2990
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      2413..2815
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             1012..1872
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             complement(3967..4737)
                     /vntifkey="4"
                     /label=kanamycin\resistance
BASE COUNT     1260 a      1158 c      1057 g      1371 t 
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcctga gcgccggtcg ctaccattac 
       61 cagttggtct ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc cgtatttcgc 
      121 gtaaggaaat ccattatgta ctatttaatt ctgcgtgaca tcccatcgat cagaccagtt 
      181 tttaatttgt gtgtttccat gtgtccagtt tggaatactc ttaacctcat tggaaatcgc 
      241 ggcataatca ctggtggtat gattgatgac cgcgtcaaca atgaccttta tgccatattc 
      301 ttcagcggct gcacacattt ctttaaattc ttgttcagta cctaagtaac ggttgccaat 
      361 ttgatacgat gtcggctgat acagccagta ccagttcgac atgcttttat ctccttgatt 
      421 cccttccttt acttggttaa tcggagatgt ctgaatggct gtatatcctg catcatgaat 
      481 atccttcata ttgtgtttta acgtattgaa cgaccaattc catgcatgaa gaatggttcc 
      541 gcttttgatc gacggtgctg taagctcatt cgatttgttc gccgtttcag cactcgcagc 
      601 cgccggtcct gccagaacca aatgaaacag caataaaaat ccagcgaata acggcagtaa 
      661 agaggttttg aatcgttttg caaacattct tgacactcct tatttgattt tttgaagact 
      721 tacttcggag tcaaaaatcc ctcttacttc attcttccgc ttcctccttt caaaccgatg 
      781 tgaagactgg agaattttgt taattcttga agacgaaagg gcctcgtgat acgcctattt 
      841 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 
      901 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 
      961 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 
     1021 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 
     1081 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 
     1141 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 
     1201 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 
     1261 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 
     1321 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 
     1381 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 
     1441 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 
     1501 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 
     1561 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 
     1621 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 
     1681 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 
     1741 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 
     1801 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 
     1861 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 
     1921 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 
     1981 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 
     2041 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 
     2101 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 
     2161 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 
     2221 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 
     2281 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 
     2341 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 
     2401 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 
     2461 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 
     2521 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 
     2581 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 
     2641 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 
     2701 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 
     2761 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 
     2821 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 
     2881 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 
     2941 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 
     3001 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct aatcggcggc 
     3061 cgcatcgatg caggctccca tcatgtttat agatataagc atttacctgg ctccaatgat 
     3121 tcggattttg atagccgatg gttttggccg acgctggatc tcttttaaca aaactgtatt 
     3181 tctcggtcct cgttacacca tcactgttcg ttccttttaa catgatggtg tatgttttgc 
     3241 caaatggatc tccttttccg attgtgaatt gatctccatc cttaaacgcc gtctctggtc 
     3301 cattattgat ttgataaacg gcttttgttg tattcgcatc tgcacgcaag gtaatcgtca 
     3361 gttgatcatt gaaagaatgt gttacacctg ttttgtaatt ctcaaggaaa acatgaggcg 
     3421 cttttgcaat atcatcagga taaagcacag ctacagacct ggcattgatc gtgcctgtca 
     3481 gtttaccatc gttcacttga aatgaacccg ctccagcttt attgtcatac ctgccatcag 
     3541 gcaattttgt tgccgtattg atagagacag aggatgaacc tgcatttgcc agcacaacgc 
     3601 catgtgagcc gcgctgattc ataaatatct ggttgtttcc attcgggttc gagagttcct 
     3661 caggctgtcc agccatcaca ttgtgaaatc tattgaccgc agtgatagcc tgatcttcaa 
     3721 ataaagcact cccgcgatcg cctatttggc ttttccccgg gaacctcaca ccatttccgc 
     3781 ctccctcagg tctggaaaag aaaagaggcg tactgcctga acgagaagct atcaccgccc 
     3841 agcctaaacg gatatcatca tcgctcatcc atgtcgacgc gccatgacag ccatgataat 
     3901 tactaatact aggagaagtt aataaatacg taaccaacat gattaacaat tattagaggt 
     3961 catcgttcaa aatggtatgc gttttgacac atccactata tatccgtgtc gttctgtcca 
     4021 ctcctgaatc ccattccaga aattctctag cgattccaga agtttctcag agtcggaaag 
     4081 ttgaccagac attacgaact ggcacagatg gtcataacct gaaggaagat ctgattgctt 
     4141 aactgcttca gttaagaccg aagcgctcgt cgtataacag atgcgatgat gcagaccaat 
     4201 caacatggca cctgccattg ctacctgtac agtcaaggat ggtagaaatg ttgtcggtcc 
     4261 ttgcacacga atattacgcc atttgcctgc atattcaaac agctcttcta cgataagggc 
     4321 acaaatcgca tcgtggaacg tttgggcttc taccgattta gcagtttgat acactttctc 
     4381 taagtatcca cctgaatcat aaatcggcaa aatagagaaa aattgaccat gtgtaagcgg 
     4441 ccaatctgat tccacctgag atgcataatc tagtagaatc tcttcgctat caaaattcac 
     4501 ttccaccttc cactcaccgg ttgtccattc atggctgaac tctgcttcct ctgttgacat 
     4561 gacacacatc atctcaatat ccgaataggg cccatcagtc tgacgaccaa gagagccata 
     4621 aacaccaata gccttaacat catccccata tttatccaat attcgttcct taatttcatg 
     4681 aacaatcttc attctttctt ctctagtcat tattattggt ccattcacta ttctcattcc 
     4741 cttttcagat aattttagat ttgcttttct aaataagaat atttggagag caccgttctt 
     4801 attcagctat taaacccatt atatcgggtt tttgagggga tttcaa 
//
