LOCUS       pMG14                   5169 bp    DNA     circular     24-OCT-2014
DEFINITION  LMBP 8465.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8465
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 24-OCT-2014
            
COMMENT     VNTDATE|663938839|
COMMENT     VNTNAME|pMG14|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pMG14" 0 5169 
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COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
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COMMENT       (CMolPar 0 0 1 4927 4926 1 5169 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 1203 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 336 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 6 87 0 367 0 2770 0 3140 0 
COMMENT            3744 0 4489 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HincII" "gtyrac" 4 0 4 599 0 4198 0 4405 0 
COMMENT            4878 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 302 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 3380 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 240 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 4196 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 351 0 0 "")) 
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COMMENT             (CStringList) 1 1 1 1 "3229..3312") 
COMMENT        #13=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 0 3313 3350 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "kanamycin/tobramycin resistance (aadD)" 4 0 1 
COMMENT             4289 5059 0 (CStringList) (CStringList) 1 1 1 1 
COMMENT             "complement(4289..5059)") 
COMMENT        #15=(CFSignal (CObList) "cggR promoter" 30 0 0 5 234 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "183..219") 
COMMENT        #16=(CFSignal (CObList) "Strep-tag II" 22 0 0 311 334 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "5' UTR of amyE" 21 0 1 1010 1125 0 
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COMMENT        #18=(CFSignal (CObList) "amyE fragment" 4 0 1 470 1009 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "5' UTR of gapA" 21 0 0 262 302 0 (CStringList) 
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COMMENT        #20=(CFSignal (CObList) "amyE fragment" 4 0 1 3396 4199 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pMG14'" 1) 
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COMMENT                          "Last Modified: 10/24/14 05:29" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                   "Standard Fields") 
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FEATURES             Location/Qualifiers
     CDS             complement(4289..5059)
                     /vntifkey="4"
                     /label=kanamycin/tobramycin\resistance\(aadD)
     promoter        5..234
                     /vntifkey="30"
                     /label=cggR\promoter
     misc_feature    complement(1010..1125)
                     /vntifkey="21"
                     /label=5'\UTR\of\amyE
     CDS             1334..2194
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      2735..3137
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        3229..3312
                     /vntifkey="30"
                     /label=lac\promoter
     CDS             complement(3396..4199)
                     /vntifkey="4"
                     /label=amyE\fragment
                     /note="fragment of amyE encoding amino acid 319-586"
     misc_feature    262..302
                     /vntifkey="21"
                     /label=5'\UTR\of\gapA
     RBS             3313..3350
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     misc_marker     311..334
                     /vntifkey="22"
                     /label=Strep-tag\II
     CDS             complement(470..1009)
                     /vntifkey="4"
                     /label=amyE\fragment
                     /note="fragment of amyE encoding amino acid 1-180"
BASE COUNT     1379 a      1209 c      1120 g      1461 t 
ORIGIN
        1 aattctttgg ataaaatgaa aacgctttaa tgaaacagcc ctttctacgg gaagggctgt 
       61 ttatattggg atgcaccatt tggcgctttc tgtataagat aaagatatat aggataaaat 
      121 attgctggat aaaacgacgc ggcatgaaaa ctctgcgaat attgtcgatg aattggctct 
      181 taacagttga ataaacaatt ccaccctgtt aaaataatta aagaaagcag aaatctgcag 
      241 aagttattaa gggatgaata atccctcaat ataaatatct ctcacttatt taaaggagga 
      301 aagcttcatg tggagccacc cgcagttcga aaaaggtacc cggggatcct ctagagtcct 
      361 gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataataat aaccgggcag 
      421 gccatgtctg cccgtatttc gcgtaaggaa atccattatg tactatttaa ttctgcgtga 
      481 catcccatcg atcagaccag tttttaattt gtgtgtttcc atgtgtccag tttggaatac 
      541 tcttaacctc attggaaatc gcggcataat cactggtggt atgattgatg accgcgtcaa 
      601 caatgacctt tatgccatat tcttcagcgg ctgcacacat ttctttaaat tcttgttcag 
      661 tacctaagta acggttgcca atttgatacg atgtcggctg atacagccag taccagttcg 
      721 acatgctttt atctccttga ttcccttcct ttacttggtt aatcggagat gtctgaatgg 
      781 ctgtatatcc tgcatcatga atatccttca tattgtgttt taacgtattg aacgaccaat 
      841 tccatgcatg aagaatggtt ccgcttttga tcgacggtgc tgtaagctca ttcgatttgt 
      901 tcgccgtttc agcactcgca gccgccggtc ctgccagaac caaatgaaac agcaataaaa 
      961 atccagcgaa taacggcagt aaagaggttt tgaatcgttt tgcaaacatt cttgacactc 
     1021 cttatttgat tttttgaaga cttacttcgg agtcaaaaat ccctcttact tcattcttcc 
     1081 gcttcctcct ttcaaaccga tgtgaagact ggagaatttt gttaattctt gaagacgaaa 
     1141 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac 
     1201 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 
     1261 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 
     1321 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 
     1381 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 
     1441 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 
     1501 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 
     1561 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 
     1621 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 
     1681 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 
     1741 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 
     1801 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 
     1861 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 
     1921 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 
     1981 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 
     2041 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 
     2101 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 
     2161 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 
     2221 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 
     2281 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 
     2341 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 
     2401 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 
     2461 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 
     2521 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 
     2581 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 
     2641 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 
     2701 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 
     2761 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 
     2821 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 
     2881 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 
     2941 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 
     3001 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 
     3061 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 
     3121 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 
     3181 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 
     3241 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 
     3301 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 
     3361 ttacgccaag ctaatcggcg gccgcatcga tgcaggctcc catcatgttt atagatataa 
     3421 gcatttacct ggctccaatg attcggattt tgatagccga tggttttggc cgacgctgga 
     3481 tctcttttaa caaaactgta tttctcggtc ctcgttacac catcactgtt cgttcctttt 
     3541 aacatgatgg tgtatgtttt gccaaatgga tctccttttc cgattgtgaa ttgatctcca 
     3601 tccttaaacg ccgtctctgg tccattattg atttgataaa cggcttttgt tgtattcgca 
     3661 tctgcacgca aggtaatcgt cagttgatca ttgaaagaat gtgttacacc tgttttgtaa 
     3721 ttctcaagga aaacatgagg cgcttttgca atatcatcag gataaagcac agctacagac 
     3781 ctggcattga tcgtgcctgt cagtttacca tcgttcactt gaaatgaacc cgctccagct 
     3841 ttattgtcat acctgccatc aggcaatttt gttgccgtat tgatagagac agaggatgaa 
     3901 cctgcatttg ccagcacaac gccatgtgag ccgcgctgat tcataaatat ctggttgttt 
     3961 ccattcgggt tcgagagttc ctcaggctgt ccagccatca cattgtgaaa tctattgacc 
     4021 gcagtgatag cctgatcttc aaataaagca ctcccgcgat cgcctatttg gcttttcccc 
     4081 gggaacctca caccatttcc gcctccctca ggtctggaaa agaaaagagg cgtactgcct 
     4141 gaacgagaag ctatcaccgc ccagcctaaa cggatatcat catcgctcat ccatgtcgac 
     4201 gcgccatgac agccatgata attactaata ctaggagaag ttaataaata cgtaaccaac 
     4261 atgattaaca attattagag gtcatcgttc aaaatggtat gcgttttgac acatccacta 
     4321 tatatccgtg tcgttctgtc cactcctgaa tcccattcca gaaattctct agcgattcca 
     4381 gaagtttctc agagtcggaa agttgaccag acattacgaa ctggcacaga tggtcataac 
     4441 ctgaaggaag atctgattgc ttaactgctt cagttaagac cgaagcgctc gtcgtataac 
     4501 agatgcgatg atgcagacca atcaacatgg cacctgccat tgctacctgt acagtcaagg 
     4561 atggtagaaa tgttgtcggt ccttgcacac gaatattacg ccatttgcct gcatattcaa 
     4621 acagctcttc tacgataagg gcacaaatcg catcgtggaa cgtttgggct tctaccgatt 
     4681 tagcagtttg atacactttc tctaagtatc cacctgaatc ataaatcggc aaaatagaga 
     4741 aaaattgacc atgtgtaagc ggccaatctg attccacctg agatgcataa tctagtagaa 
     4801 tctcttcgct atcaaaattc acttccacct tccactcacc ggttgtccat tcatggctga 
     4861 actctgcttc ctctgttgac atgacacaca tcatctcaat atccgaatag ggcccatcag 
     4921 tctgacgacc aagagagcca taaacaccaa tagccttaac atcatcccca tatttatcca 
     4981 atattcgttc cttaatttca tgaacaatct tcattctttc ttctctagtc attattattg 
     5041 gtccattcac tattctcatt cccttttcag ataattttag atttgctttt ctaaataaga 
     5101 atatttggag agcaccgttc ttattcagct attaaaccca ttatatcggg tttttgaggg 
     5161 gatttcaag 
//
