LOCUS       pENTR3C-mBeclin1-T575C-T587C+stop      3639 bp    DNA     circular SYN 09-FEB-2012
DEFINITION  LMBP 8536.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8536
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
DBSOURCE    GenBank
            Unknown
            Unclassified
FEATURES             Location/Qualifiers
     Source          join(<1855..3639,1..>496)
                     /invitrogen="563"
                     /label=Source_1
     Vector          join(1855..3639,1..496)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
     Terminator      complement(106..149)
                     /invitrogen="2580000"
                     /label="rrnB T1 transcription terminator"
     Terminator      complement(281..308)
                     /invitrogen="2590000"
                     /label="rrnB T2 transcription terminator"
     Misc._recombina 358..457
                     /invitrogen="2670000"
                     /label=attL1
     CDS             505..1851
                     /origin="mBeclin + stop + primers (1.384bp linear)
                     Fragment: from EcoRI cut at 11^12 to XhoI cut at
                     1369^1370, 1.358bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /label="mBeclin1_T575C_T587C + stop"
     Misc._recombina complement(1868..1967)
                     /invitrogen="2680000"
                     /label=attL2
     CDS             2090..2899
                     /invitrogen="1210000"
                     /label=Kan(R)
     Replication_ori 2963..3636
                     /invitrogen="2540000"
                     /label="pUC origin"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG
       61 GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT
      121 CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
      181 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG
      241 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT
      301 GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA
      361 ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC
      421 TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG
      481 ACTGGATCCG GTACCGAATT CACCATGGAG GGGTCTAAGG CGTCCAGCAG CACCATGCAG
      541 GTGAGCTTCG TGTGCCAGCG CTGTAGCCAG CCTCTGAAAC TGGACACGAG CTTCAAGATC
      601 CTGGACCGGG TCACCATCCA GGAACTCACA GCTCCATTAC TTACCACAGC CCAGGCGAAA
      661 CCAGGAGAGA CCCAGGAGGA AGAGGCTAAC TCAGGAGAGG AGCCATTTAT TGAAACTCGC
      721 CAGGATGGTG TCTCTCGAAG ATTCATCCCC CCAGCCAGGA TGATGTCTAC AGAAAGTGCT
      781 AATAGCTTCA CTCTGATCGG GGAGGCATCT GATGGTGGCA CCATGGAGAA CCTCAGCCGG
      841 AGACTCAAGG TCACTGGAGA CCTGTTTGAC ATCATGTCGG GCCAGACCGA TGTGGATCAC
      901 CCGCTGTGTG AGGAATGCAC AGACACTCTT TTAGACCAGC TGGACACTCA GCTCAATGTC
      961 ACTGAGAATG AATGTCAGAA CTACAAACGC TGTTTGGAGA TCCTAGAGCA GATGAATGAG
     1021 GATGACAGTG AGCAGCTGCA GAGGGAGCTG AAGGAGCTGG CCTTGGAGGA GGAGAGGCCG
     1081 ATCCAGGAGC CGGAAGATGT GGAAAAGAAC CGCAAGGTGG TGGCAGAGAA CCTGGAGAAG
     1141 GTCCAGGCTG AGGCGGAGAG ATTGGACCAG GAGGAAGCTC AGTACCAGCG GGAGTATAGT
     1201 GAGTTTAAAA GGCAGCAGCT GGAGTTGGAT GACGAACTCA AGAGTGTGGA GAACCAGGTG
     1261 CGCTACGCCC AGATCCAGCT GGACAAGCTC AAGAAAACCA ATGTCTTCAA TGCCACCTTC
     1321 CACATCTGGC ACAGCGGACA GTTTGGCACA ATCAATAATT TCAGACTGGG TCGCTTGCCC
     1381 AGTGTTCCTG TGGAGTGGAA TGAAATCAAT GCTGCCTGGG GCCAGACAGT GCTGCTGCTC
     1441 CATGCTTTGG CCAATAAGAT GGGTCTGAAG TTTCAGAGGT ACCGACTTGT TCCCTATGGA
     1501 AATCATTCCT ATCTGGAGTC TCTGACAGAC AAATCTAAGG AGTTGCCGTT ATACTGTTCT
     1561 GGGGGTTTGC GGTTTTTCTG GGACAACAAG TTTGACCATG CAATGGTAGC TTTTCTGGAC
     1621 TGTGTGCAGC AGTTCAAAGA AGAGGTGGAA AAAGGAGAGA CTCGATTTTG TCTTCCGTAC
     1681 AGGATGGACG TGGAGAAAGG CAAGATTGAA GACACTGGAG GCAGTGGCGG CTCCTATTCC
     1741 ATCAAAACCC AGTTTAACTC GGAGGAGCAG TGGACAAAAG CGCTCAAGTT CATGCTGACC
     1801 AATCTCAAGT GGGGTCTTGC CTGGGTGTCC TCACAGTTCT ATAACAAGTG ACACTCGAGA
     1861 TATCTAGACC CAGCTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG CTTATCAATT
     1921 TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGCCA TCCAGCTGCA
     1981 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA ATATATCATC
     2041 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT
     2101 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA
     2161 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA
     2221 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC
     2281 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA
     2341 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC
     2401 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT
     2461 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT
     2521 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT
     2581 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT
     2641 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT
     2701 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA
     2761 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG
     2821 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT
     2881 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACATTATT CAGATTGGGC
     2941 CCCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT
     3001 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG
     3061 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA
     3121 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT
     3181 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA
     3241 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC
     3301 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT
     3361 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA
     3421 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG
     3481 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT
     3541 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT
     3601 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT
//
