LOCUS pLenti6-mBeclin1-C-Frag-122-Flag-puro 8200 bp DNA circular SYN 09-FEB-2012 DEFINITION LMBP 8550. SOURCE ORGANISM COMMENT Accession number: LMBP 8550 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) DBSOURCE GenBank Unknown Unclassified FEATURES Location/Qualifiers Source join(<1049..>1071,<1..>56) /invitrogen="563" /label=Source_1 Misc._recombina join(8194..8200,1..14) /label=attB1 Vector join(1049..>1071,<1..56) /origin="pENTR3C (2.723bp circular) Targetvector: from XhoI cut at 938^939 to EcoRI cut at 496^497,2.281bp Orientation: original Plusstrand 5': no change Plus strand 3': nochange Minus strand 5': no change Minusstrand 3': no change" CDS 65..1045 /origin="mBeclin + stop + primers (1.384bp linear)Fragment: from EcoRI cut at 11^12 to XhoI cut at1369^1370, 1.358bp Orientation: originalPlus strand 5': no change Plus strand 3': nochange Minus strand 5': no changeMinus strand 3': no change" /label="mBeclin-C- Frag_122 - stop" /label="mBeclin-C- Frag_122 - stop" Misc._recombina complement(1065..1085) /label=attB2 Misc._feature 1129..1153 /label="FLAG tag" /label="FLAG tag" CDS 1166..1174 /invitrogen="123000001" /note="Oriinal location: 8422..8430" /label="3 stops" /label="3 stops" Promoter 1211..1520 /invitrogen="2290000" /note="Oriinal location: 8467..8776" /label="SV40 early promoter" /label="SV40 early promoter" CDS 1580..2182 /invitrogen="1050000" /note="Oriinal location: 1..603" /label=Puro(R) LTR 2273..2326 /invitrogen="1410000" /note="Oriinal location: 694..747" /label=deltaU3 LTR 2274..2507 /invitrogen="1390000" /note="Oriinal location: 695..928" /label="deltaU3/3' LTR" /label="deltaU3/3' LTR" LTR 2327..2507 /invitrogen="1430000" /note="Oriinal location: 748..928" /label="HIV-1 3' LTR" /label="HIV-1 3' LTR" PolyA_signal 2579..2709 /invitrogen="1840000" /note="Oriinal location: 1000..1130" /label="SV40 pA" /label="SV40 pA" Replication_ori 3082..3537 /invitrogen="2520000" /note="Oriinal location: 1503..1958" /label="f1 origin" /label="f1 origin" Promoter 3569..3667 /invitrogen="2330000" /note="Oriinal location: 1990..2088" /label="bla promoter" /label="bla promoter" CDS 3668..4528 /invitrogen="1020000" /note="Oriinal location: 2089..2949" /label=Amp(R) Replication_ori 4673..5346 /invitrogen="2540000" /note="Oriinal location: 3094..3767" /label="pUC origin" /label="pUC origin" Source 5753^5754 /invitrogen="765" /note="Oriinal location: 4175..4174" /label=Source_1 Promoter 5754..5982 /invitrogen="2270000" /note="Oriinal location: 4175..4403" /label="RSV promoter" /label="RSV promoter" Promoter 5754..6163 /invitrogen="2280000" /note="Oriinal location: 4175..4584" /label="RSV/5' LTR hybrid promoter" /label="RSV/5' LTR hybrid promoter" LTR 5983..6163 /invitrogen="1430000" /note="Oriinal location: 4404..4584" /label="HIV-1 5' LTR" /label="HIV-1 5' LTR" Misc._feature 6273 /invitrogen="123000012" /note="Oriinal location: 4694..4694" /label="splice donor" /label="splice donor" Misc._binding 6274..6318 /invitrogen="1780000" /note="Oriinal location: 4695..4739" /label="HIV-1 psi packaging signal" /label="HIV-1 psi packaging signal" Protein_binding 6828..7061 /invitrogen="2440000" /note="Oriinal location: 5249..5482" /label="HIV-1 Rev response element" /label="HIV-1 Rev response element" Misc._feature 7409 /invitrogen="123000013" /note="Oriinal location: 5830..5830" /label="splice acceptor" /label="splice acceptor" Misc._feature 7437 /invitrogen="123000014" /note="Oriinal location: 5858..5858" /label="splice acceptor" /label="splice acceptor" Promoter 7562..8144 /invitrogen="2170000" /note="Oriinal location: 5983..6565" /label="CMV promoter" /label="CMV promoter" TATA_box 8062..8065 /invitrogen="123000002" /note="Oriinal location: 6483..6486" /label="TATA box" /label="TATA box" ORIGIN 1 GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG ACTGGATCCG GTACCGAATT 61 CACCATGATC ATGTCGGGCC AGACCGATGT GGATCACCCG CTGTGTGAGG AATGCACAGA 121 CACTCTTTTA GACCAGCTGG ACACTCAGCT CAATGTCACT GAGAATGAAT GTCAGAACTA 181 CAAACGCTGT TTGGAGATCC TAGAGCAGAT GAATGAGGAT GACAGTGAGC AGCTGCAGAG 241 GGAGCTGAAG GAGCTGGCCT TGGAGGAGGA GAGGCTGATC CAGGAGCTGG AAGATGTGGA 301 AAAGAACCGC AAGGTGGTGG CAGAGAACCT GGAGAAGGTC CAGGCTGAGG CGGAGAGATT 361 GGACCAGGAG GAAGCTCAGT ACCAGCGGGA GTATAGTGAG TTTAAAAGGC AGCAGCTGGA 421 GTTGGATGAC GAACTCAAGA GTGTGGAGAA CCAGGTGCGC TACGCCCAGA TCCAGCTGGA 481 CAAGCTCAAG AAAACCAATG TCTTCAATGC CACCTTCCAC ATCTGGCACA GCGGACAGTT 541 TGGCACAATC AATAATTTCA GACTGGGTCG CTTGCCCAGT GTTCCTGTGG AGTGGAATGA 601 AATCAATGCT GCCTGGGGCC AGACAGTGCT GCTGCTCCAT GCTTTGGCCA ATAAGATGGG 661 TCTGAAGTTT CAGAGGTACC GACTTGTTCC CTATGGAAAT CATTCCTATC TGGAGTCTCT 721 GACAGACAAA TCTAAGGAGT TGCCGTTATA CTGTTCTGGG GGTTTGCGGT TTTTCTGGGA 781 CAACAAGTTT GACCATGCAA TGGTAGCTTT TCTGGACTGT GTGCAGCAGT TCAAAGAAGA 841 GGTGGAAAAA GGAGAGACTC GATTTTGTCT TCCGTACAGG ATGGACGTGG AGAAAGGCAA 901 GATTGAAGAC ACTGGAGGCA GTGGCGGCTC CTATTCCATC AAAACCCAGT TTAACTCGGA 961 GGAGCAGTGG ACAAAAGCGC TCAAGTTCAT GCTGACCAAT CTCAAGTGGG GTCTTGCCTG 1021 GGTGTCCTCA CAGTTCTATA ACAAGCACTC GAGATATCTA GACCCAGCTT TCTTGTACAA 1081 AGTGGTTGAT ATCCAGCACA GTGGCGGCCG CTCGAGTCTA GAGGGCCCGG ACTACAAGGA 1141 TGACGATGAC AAAACGCGTA CCGGTTAGTA ATGAGTTTGG AATTAATTCT GTGGAATGTG 1201 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGGCA GGCAGAAGTA TGCAAAGCAT 1261 GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG 1321 TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT 1381 CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT 1441 TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG 1501 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG 1561 ATCTGATCAG CACGTGACCA TGGCCACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG 1621 CGACGACGTC CCCCGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC 1681 GCGCCACACC GTCGACCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT 1741 CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT 1801 GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCTC 1861 GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT 1921 GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA 1981 CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG 2041 CGCTGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG 2101 GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT 2161 GACCCGCAAG CCCGGTGCCT GAGCTAAGCA CAATTCGAGC TCGGTACCTT TAAGACCAAT 2221 GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG 2281 GCTAATTCAC TCCCAACGAA GACAAGATCT GCTTTTTGCT TGTACTGGGT CTCTCTGGTT 2341 AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA 2401 ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA 2461 CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTA GTAGTTCATG 2521 TCATCTTATT ATTCAGTATT TATAACTTGC AAAGAAATGA ATATCAGAGA GTGAGAGGAA 2581 CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA 2641 TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA 2701 TCATGTCTGG CTCTAGCTAT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC 2761 AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG 2821 GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGG 2881 ACGTACCCAA TTCGCCCTAT AGTGAGTCGT ATTACGCGCG CTCACTGGCC GTCGTTTTAC 2941 AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC 3001 CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC 3061 GCAGCCTGAA TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 3121 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 3181 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 3241 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 3301 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 3361 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 3421 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 3481 AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTAGG 3541 TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC 3601 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG 3661 GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG 3721 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT 3781 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT 3841 TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT 3901 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA 3961 TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG 4021 AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC 4081 AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC 4141 TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC 4201 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC 4261 TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT 4321 TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG 4381 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT 4441 TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT 4501 AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA 4561 GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA 4621 TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 4681 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 4741 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 4801 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC 4861 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 4921 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 4981 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 5041 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG 5101 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 5161 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 5221 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 5281 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 5341 TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA 5401 GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 5461 AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG 5521 CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT 5581 GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT 5641 GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC 5701 CAAGCGCGCA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG GAGCTGCAAG CTTAATGTAG 5761 TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA 5821 CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC 5881 TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT 5941 TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACATAA ACGGGTCTCT CTGGTTAGAC 6001 CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA 6061 AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG 6121 AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG 6181 ACTTGAAAGC GAAAGGGAAA CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG 6241 CGCGCACGGC AAGAGGCGAG GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG 6301 GAGGCTAGAA GGAGAGAGAT GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT 6361 CGCGATGGGA AAAAATTCGG TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA 6421 TAGTATGGGC AAGCAGGGAG CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT 6481 CAGAAGGCTG TAGACAAATA CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG 6541 AACTTAGATC ATTATATAAT ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA 6601 TAAAAGACAC CAAGGAAGCT TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA 6661 CCGCACAGCA AGCGGCCGCT GATCTTCAGA CCTGGAGGAG GAGATATGAG GGACAATTGG 6721 AGAAGTGAAT TATATAAATA TAAAGTAGTA AAAATTGAAC CATTAGGAGT AGCACCCACC 6781 AAGGCAAAGA GAAGAGTGGT GCAGAGAGAA AAAAGAGCAG TGGGAATAGG AGCTTTGTTC 6841 CTTGGGTTCT TGGGAGCAGC AGGAAGCACT ATGGGCGCAG CGTCAATGAC GCTGACGGTA 6901 CAGGCCAGAC AATTATTGTC TGGTATAGTG CAGCAGCAGA ACAATTTGCT GAGGGCTATT 6961 GAGGCGCAAC AGCATCTGTT GCAACTCACA GTCTGGGGCA TCAAGCAGCT CCAGGCAAGA 7021 ATCCTGGCTG TGGAAAGATA CCTAAAGGAT CAACAGCTCC TGGGGATTTG GGGTTGCTCT 7081 GGAAAACTCA TTTGCACCAC TGCTGTGCCT TGGAATGCTA GTTGGAGTAA TAAATCTCTG 7141 GAACAGATTT GGAATCACAC GACCTGGATG GAGTGGGACA GAGAAATTAA CAATTACACA 7201 AGCTTAATAC ACTCCTTAAT TGAAGAATCG CAAAACCAGC AAGAAAAGAA TGAACAAGAA 7261 TTATTGGAAT TAGATAAATG GGCAAGTTTG TGGAATTGGT TTAACATAAC AAATTGGCTG 7321 TGGTATATAA AATTATTCAT AATGATAGTA GGAGGCTTGG TAGGTTTAAG AATAGTTTTT 7381 GCTGTACTTT CTATAGTGAA TAGAGTTAGG CAGGGATATT CACCATTATC GTTTCAGACC 7441 CACCTCCCAA CCCCGAGGGG ACCCGACAGG CCCGAAGGAA TAGAAGAAGA AGGTGGAGAG 7501 AGAGACAGAG ACAGATCCAT TCGATTAGTG AACGGATCTC GACGGTATCG ATAAGCTTGG 7561 GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC 7621 CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT 7681 TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT 7741 CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT 7801 GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC 7861 GCTATTACCA TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC 7921 TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA 7981 AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC CATTGACGCA AATGGGCGGT 8041 AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCGCC 8101 TGGAGACGCC ATCCACGCTG TTTTGACCTC CATAGAAGAC ACCGACTCTA GAGGATCCAC 8161 TAGTCCAGTG TGGTGGAATT CTGCAGATAT CAACAAGTTT //