LOCUS       pENTR3C-mBeclin1-D122A-stop      3636 bp    DNA     circular SYN 09-FEB-2012
DEFINITION  LMBP 8558.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8558
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
DBSOURCE    GenBank
            Unknown
            Unclassified
FEATURES             Location/Qualifiers
     Source          join(<1852..3636,1..>496)
                     /invitrogen="563"
                     /label=Source_1
     Vector          join(1852..3636,1..496)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
     Terminator      complement(106..149)
                     /invitrogen="2580000"
                     /label="rrnB T1 transcription terminator"
     Terminator      complement(281..308)
                     /invitrogen="2590000"
                     /label="rrnB T2 transcription terminator"
     Misc._recombina 358..457
                     /invitrogen="2670000"
                     /label=attL1
     CDS             505..1848
                     /origin="mBeclin + stop + primers (1.384bp linear)
                     Fragment: from EcoRI cut at 11^12 to XhoI cut at
                     1369^1370, 1.358bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /label="mBeclin_uncleavable_pos3  - stop"
     Misc._recombina complement(1865..1964)
                     /invitrogen="2680000"
                     /label=attL2
     CDS             2087..2896
                     /invitrogen="1210000"
                     /label=Kan(R)
     Replication_ori 2960..3633
                     /invitrogen="2540000"
                     /label="pUC origin"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG
       61 GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT
      121 CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
      181 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG
      241 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT
      301 GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA
      361 ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC
      421 TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG
      481 ACTGGATCCG GTACCGAATT CACCATGGAG GGGTCTAAGG CGTCCAGCAG CACCATGCAG
      541 GTGAGCTTCG TGTGCCAGCG CTGTAGCCAG CCTCTGAAAC TGGACACGAG CTTCAAGATC
      601 CTGGACCGGG TCACCATCCA GGAACTCACA GCTCCATTAC TTACCACAGC CCAGGCGAAA
      661 CCAGGAGAGA CCCAGGAGGA AGAGGCTAAC TCAGGAGAGG AGCCATTTAT TGAAACTCGC
      721 CAGGATGGTG TCTCTCGAAG ATTCATCCCC CCAGCCAGGA TGATGTCTAC AGAAAGTGCT
      781 AATAGCTTCA CTCTGATCGG GGAGGCATCT GATGGTGGCA CCATGGAGAA CCTCAGCCGG
      841 AGACTCAAGG TCACTGGAGA CCTGTTTGCC ATCATGTCGG GCCAGACCGA TGTGGATCAC
      901 CCGCTGTGTG AGGAATGCAC AGACACTCTT TTAGACCAGC TGGACACTCA GCTCAATGTC
      961 ACTGAGAATG AATGTCAGAA CTACAAACGC TGTTTGGAGA TCCTAGAGCA GATGAATGAG
     1021 GATGACAGTG AGCAGCTGCA GAGGGAGCTG AAGGAGCTGG CCTTGGAGGA GGAGAGGCTG
     1081 ATCCAGGAGC TGGAAGATGT GGAAAAGAAC CGCAAGGTGG TGGCAGAGAA CCTGGAGAAG
     1141 GTCCAGGCTG AGGCGGAGAG ATTGGACCAG GAGGAAGCTC AGTACCAGCG GGAGTATAGT
     1201 GAGTTTAAAA GGCAGCAGCT GGAGTTGGAT GACGAACTCA AGAGTGTGGA GAACCAGGTG
     1261 CGCTACGCCC AGATCCAGCT GGACAAGCTC AAGAAAACCA ATGTCTTCAA TGCCACCTTC
     1321 CACATCTGGC ACAGCGGACA GTTTGGCACA ATCAATAATT TCAGACTGGG TCGCTTGCCC
     1381 AGTGTTCCTG TGGAGTGGAA TGAAATCAAT GCTGCCTGGG GCCAGACAGT GCTGCTGCTC
     1441 CATGCTTTGG CCAATAAGAT GGGTCTGAAG TTTCAGAGGT ACCGACTTGT TCCCTATGGA
     1501 AATCATTCCT ATCTGGAGTC TCTGACAGAC AAATCTAAGG AGTTGCCGTT ATACTGTTCT
     1561 GGGGGTTTGC GGTTTTTCTG GGACAACAAG TTTGACCATG CAATGGTAGC TTTTCTGGAC
     1621 TGTGTGCAGC AGTTCAAAGA AGAGGTGGAA AAAGGAGAGA CTCGATTTTG TCTTCCGTAC
     1681 AGGATGGACG TGGAGAAAGG CAAGATTGAA GACACTGGAG GCAGTGGCGG CTCCTATTCC
     1741 ATCAAAACCC AGTTTAACTC GGAGGAGCAG TGGACAAAAG CGCTCAAGTT CATGCTGACC
     1801 AATCTCAAGT GGGGTCTTGC CTGGGTGTCC TCACAGTTCT ATAACAAGCA CTCGAGATAT
     1861 CTAGACCCAG CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT
     1921 TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGCAGCT
     1981 CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAAATA TATCATCATG
     2041 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA
     2101 ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA
     2161 ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC
     2221 CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA
     2281 TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT
     2341 TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCATT
     2401 CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT
     2461 CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT
     2521 TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA
     2581 TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC
     2641 ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA
     2701 CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA
     2761 GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT
     2821 TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT
     2881 CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CATTATTCAG ATTGGGCCCC
     2941 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT
     3001 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT
     3061 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT
     3121 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC
     3181 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA
     3241 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG
     3301 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG
     3361 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG
     3421 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA
     3481 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT
     3541 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG
     3601 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT
//
