LOCUS       pLenti6-mBeclin1-C-Frag-147-V5-puro      8149 bp    DNA     circular SYN 09-FEB-2012
DEFINITION  LMBP 8562.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8562
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
DBSOURCE    GenBank
            Unknown
            Unclassified
FEATURES             Location/Qualifiers
     Source          join(<974..>996,<1..>56)
                     /invitrogen="563"
                     /label=Source_1
     Misc._recombina join(8143..8149,1..14)
                     /label=attB1
     Vector          join(974..>996,<1..56)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
     CDS             65..970
                     /origin="mBeclin + stop + primers (1.384bp linear)
                     Fragment: from EcoRI cut at 11^12 to XhoI cut at
                     1369^1370, 1.358bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /label="mBeclin-C- Frag_147  - stop"
     Misc._recombina complement(990..1010)
                     /label=attB2
     CDS             1064..1105
                     /invitrogen="1350000"
                     /note="Oriinal location: 8371..8412"
                     /label="V5 epitope"
     CDS             1115..1123
                     /invitrogen="123000001"
                     /note="Oriinal location: 8422..8430"
                     /label="3 stops"
     Promoter        1160..1469
                     /invitrogen="2290000"
                     /note="Oriinal location: 8467..8776"
                     /label="SV40 early promoter"
     CDS             1529..2131
                     /invitrogen="1050000"
                     /note="Oriinal location: 1..603"
                     /label=Puro(R)
     LTR             2222..2275
                     /invitrogen="1410000"
                     /note="Oriinal location: 694..747"
                     /label=deltaU3
     LTR             2223..2456
                     /invitrogen="1390000"
                     /note="Oriinal location: 695..928"
                     /label="deltaU3/3' LTR"
     LTR             2276..2456
                     /invitrogen="1430000"
                     /note="Oriinal location: 748..928"
                     /label="HIV-1 3' LTR"
     PolyA_signal    2528..2658
                     /invitrogen="1840000"
                     /note="Oriinal location: 1000..1130"
                     /label="SV40 pA"
     Replication_ori 3031..3486
                     /invitrogen="2520000"
                     /note="Oriinal location: 1503..1958"
                     /label="f1 origin"
     Promoter        3518..3616
                     /invitrogen="2330000"
                     /note="Oriinal location: 1990..2088"
                     /label="bla promoter"
     CDS             3617..4477
                     /invitrogen="1020000"
                     /note="Oriinal location: 2089..2949"
                     /label=Amp(R)
     Replication_ori 4622..5295
                     /invitrogen="2540000"
                     /note="Oriinal location: 3094..3767"
                     /label="pUC origin"
     Source          5702^5703
                     /invitrogen="765"
                     /note="Oriinal location: 4175..4174"
                     /label=Source_1
     Promoter        5703..5931
                     /invitrogen="2270000"
                     /note="Oriinal location: 4175..4403"
                     /label="RSV promoter"
     Promoter        5703..6112
                     /invitrogen="2280000"
                     /note="Oriinal location: 4175..4584"
                     /label="RSV/5' LTR hybrid promoter"
     LTR             5932..6112
                     /invitrogen="1430000"
                     /note="Oriinal location: 4404..4584"
                     /label="HIV-1 5' LTR"
     Misc._feature   6222
                     /invitrogen="123000012"
                     /note="Oriinal location: 4694..4694"
                     /label="splice donor"
     Misc._binding   6223..6267
                     /invitrogen="1780000"
                     /note="Oriinal location: 4695..4739"
                     /label="HIV-1 psi packaging signal"
     Protein_binding 6777..7010
                     /invitrogen="2440000"
                     /note="Oriinal location: 5249..5482"
                     /label="HIV-1 Rev response element"
     Misc._feature   7358
                     /invitrogen="123000013"
                     /note="Oriinal location: 5830..5830"
                     /label="splice acceptor"
     Misc._feature   7386
                     /invitrogen="123000014"
                     /note="Oriinal location: 5858..5858"
                     /label="splice acceptor"
     Promoter        7511..8093
                     /invitrogen="2170000"
                     /note="Oriinal location: 5983..6565"
                     /label="CMV promoter"
     TATA_box        8011..8014
                     /invitrogen="123000002"
                     /note="Oriinal location: 6483..6486"
                     /label="TATA box"
ORIGIN
        1 GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG ACTGGATCCG GTACCGAATT
       61 CACCATGACT CAGCTCAATG TCACTGAGAA TGAATGTCAG AACTACAAAC GCTGTTTGGA
      121 GATCCTAGAG CAGATGAATG AGGATGACAG TGAGCAGCTG CAGAGGGAGC TGAAGGAGCT
      181 GGCCTTGGAG GAGGAGAGGC TGATCCAGGA GCTGGAAGAT GTGGAAAAGA ACCGCAAGGT
      241 GGTGGCAGAG AACCTGGAGA AGGTCCAGGC TGAGGCGGAG AGATTGGACC AGGAGGAAGC
      301 TCAGTACCAG CGGGAGTATA GTGAGTTTAA AAGGCAGCAG CTGGAGTTGG ATGACGAACT
      361 CAAGAGTGTG GAGAACCAGG TGCGCTACGC CCAGATCCAG CTGGACAAGC TCAAGAAAAC
      421 CAATGTCTTC AATGCCACCT TCCACATCTG GCACAGCGGA CAGTTTGGCA CAATCAATAA
      481 TTTCAGACTG GGTCGCTTGC CCAGTGTTCC TGTGGAGTGG AATGAAATCA ATGCTGCCTG
      541 GGGCCAGACA GTGCTGCTGC TCCATGCTTT GGCCAATAAG ATGGGTCTGA AGTTTCAGAG
      601 GTACCGACTT GTTCCCTATG GAAATCATTC CTATCTGGAG TCTCTGACAG ACAAATCTAA
      661 GGAGTTGCCG TTATACTGTT CTGGGGGTTT GCGGTTTTTC TGGGACAACA AGTTTGACCA
      721 TGCAATGGTA GCTTTTCTGG ACTGTGTGCA GCAGTTCAAA GAAGAGGTGG AAAAAGGAGA
      781 GACTCGATTT TGTCTTCCGT ACAGGATGGA CGTGGAGAAA GGCAAGATTG AAGACACTGG
      841 AGGCAGTGGC GGCTCCTATT CCATCAAAAC CCAGTTTAAC TCGGAGGAGC AGTGGACAAA
      901 AGCGCTCAAG TTCATGCTGA CCAATCTCAA GTGGGGTCTT GCCTGGGTGT CCTCACAGTT
      961 CTATAACAAG CACTCGAGAT ATCTAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCCA
     1021 GCACAGTGGC GGCCGCTCGA GTCTAGAGGG CCCGCGGTTC GAAGGTAAGC CTATCCCTAA
     1081 CCCTCTCCTC GGTCTCGATT CTACGCGTAC CGGTTAGTAA TGAGTTTGGA ATTAATTCTG
     1141 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGGCAG GCAGAAGTAT
     1201 GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC
     1261 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC
     1321 TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT
     1381 AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA
     1441 GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC
     1501 CATTTTCGGA TCTGATCAGC ACGTGACCAT GGCCACCGAG TACAAGCCCA CGGTGCGCCT
     1561 CGCCACCCGC GACGACGTCC CCCGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA
     1621 CCCCGCCACG CGCCACACCG TCGACCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA
     1681 AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG
     1741 CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA
     1801 GATCGGCTCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA
     1861 AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT
     1921 CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC
     1981 GGCCGAGCGC GCTGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT
     2041 CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC
     2101 CTGGTGCATG ACCCGCAAGC CCGGTGCCTG AGCTAAGCAC AATTCGAGCT CGGTACCTTT
     2161 AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG
     2221 ACTGGAAGGG CTAATTCACT CCCAACGAAG ACAAGATCTG CTTTTTGCTT GTACTGGGTC
     2281 TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT
     2341 TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA
     2401 CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGTAG
     2461 TAGTTCATGT CATCTTATTA TTCAGTATTT ATAACTTGCA AAGAAATGAA TATCAGAGAG
     2521 TGAGAGGAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA
     2581 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA
     2641 TGTATCTTAT CATGTCTGGC TCTAGCTATC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
     2701 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA
     2761 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA
     2821 GGCCTAGGGA CGTACCCAAT TCGCCCTATA GTGAGTCGTA TTACGCGCGC TCACTGGCCG
     2881 TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG
     2941 CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC
     3001 AACAGTTGCG CAGCCTGAAT GGCGAATGGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG
     3061 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC
     3121 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA
     3181 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC
     3241 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT
     3301 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA
     3361 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT
     3421 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA
     3481 CAATTTAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA
     3541 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA
     3601 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC
     3661 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA
     3721 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT
     3781 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG
     3841 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA
     3901 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT
     3961 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT
     4021 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA
     4081 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA
     4141 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA
     4201 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC
     4261 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC
     4321 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG
     4381 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT
     4441 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA
     4501 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT
     4561 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA
     4621 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG
     4681 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC
     4741 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT
     4801 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC
     4861 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT
     4921 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG
     4981 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT
     5041 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG
     5101 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG
     5161 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG
     5221 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG
     5281 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC
     5341 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT
     5401 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT
     5461 TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC
     5521 AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC
     5581 TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA
     5641 TGATTACGCC AAGCGCGCAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG AGCTGCAAGC
     5701 TTAATGTAGT CTTATGCAAT ACTCTTGTAG TCTTGCAACA TGGTAACGAT GAGTTAGCAA
     5761 CATGCCTTAC AAGGAGAGAA AAAGCACCGT GCATGCCGAT TGGTGGAAGT AAGGTGGTAC
     5821 GATCGTGCCT TATTAGGAAG GCAACAGACG GGTCTGACAT GGATTGGACG AACCACTGAA
     5881 TTGCCGCATT GCAGAGATAT TGTATTTAAG TGCCTAGCTC GATACATAAA CGGGTCTCTC
     5941 TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG
     6001 CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT
     6061 GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTGGCGCC
     6121 CGAACAGGGA CTTGAAAGCG AAAGGGAAAC CAGAGGAGCT CTCTCGACGC AGGACTCGGC
     6181 TTGCTGAAGC GCGCACGGCA AGAGGCGAGG GGCGGCGACT GGTGAGTACG CCAAAAATTT
     6241 TGACTAGCGG AGGCTAGAAG GAGAGAGATG GGTGCGAGAG CGTCAGTATT AAGCGGGGGA
     6301 GAATTAGATC GCGATGGGAA AAAATTCGGT TAAGGCCAGG GGGAAAGAAA AAATATAAAT
     6361 TAAAACATAT AGTATGGGCA AGCAGGGAGC TAGAACGATT CGCAGTTAAT CCTGGCCTGT
     6421 TAGAAACATC AGAAGGCTGT AGACAAATAC TGGGACAGCT ACAACCATCC CTTCAGACAG
     6481 GATCAGAAGA ACTTAGATCA TTATATAATA CAGTAGCAAC CCTCTATTGT GTGCATCAAA
     6541 GGATAGAGAT AAAAGACACC AAGGAAGCTT TAGACAAGAT AGAGGAAGAG CAAAACAAAA
     6601 GTAAGACCAC CGCACAGCAA GCGGCCGCTG ATCTTCAGAC CTGGAGGAGG AGATATGAGG
     6661 GACAATTGGA GAAGTGAATT ATATAAATAT AAAGTAGTAA AAATTGAACC ATTAGGAGTA
     6721 GCACCCACCA AGGCAAAGAG AAGAGTGGTG CAGAGAGAAA AAAGAGCAGT GGGAATAGGA
     6781 GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA GGAAGCACTA TGGGCGCAGC GTCAATGACG
     6841 CTGACGGTAC AGGCCAGACA ATTATTGTCT GGTATAGTGC AGCAGCAGAA CAATTTGCTG
     6901 AGGGCTATTG AGGCGCAACA GCATCTGTTG CAACTCACAG TCTGGGGCAT CAAGCAGCTC
     6961 CAGGCAAGAA TCCTGGCTGT GGAAAGATAC CTAAAGGATC AACAGCTCCT GGGGATTTGG
     7021 GGTTGCTCTG GAAAACTCAT TTGCACCACT GCTGTGCCTT GGAATGCTAG TTGGAGTAAT
     7081 AAATCTCTGG AACAGATTTG GAATCACACG ACCTGGATGG AGTGGGACAG AGAAATTAAC
     7141 AATTACACAA GCTTAATACA CTCCTTAATT GAAGAATCGC AAAACCAGCA AGAAAAGAAT
     7201 GAACAAGAAT TATTGGAATT AGATAAATGG GCAAGTTTGT GGAATTGGTT TAACATAACA
     7261 AATTGGCTGT GGTATATAAA ATTATTCATA ATGATAGTAG GAGGCTTGGT AGGTTTAAGA
     7321 ATAGTTTTTG CTGTACTTTC TATAGTGAAT AGAGTTAGGC AGGGATATTC ACCATTATCG
     7381 TTTCAGACCC ACCTCCCAAC CCCGAGGGGA CCCGACAGGC CCGAAGGAAT AGAAGAAGAA
     7441 GGTGGAGAGA GAGACAGAGA CAGATCCATT CGATTAGTGA ACGGATCTCG ACGGTATCGA
     7501 TAAGCTTGGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC
     7561 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG
     7621 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT
     7681 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC
     7741 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA
     7801 TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG
     7861 CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT
     7921 TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA
     7981 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT
     8041 CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGACA CCGACTCTAG
     8101 AGGATCCACT AGTCCAGTGT GGTGGAATTC TGCAGATATC AACAAGTTT
//
