LOCUS       pLenti6-mBeclin1-C-Frag-122-tdTomato-V5-puro        9649 bp    DNA     circular SYN 24-AUG-2016
DEFINITION  LMBP 8564.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8564
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Unknown Unclassified.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11384117|
COMMENT     VNTDBDATE|-11384117|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLenti6-mBeclin1-C-Frag-122-tdTomato-V5-puro|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pLenti6-mBeclin1-C-Frag-122-tdTomato-V5-puro" 0 9649 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 6755 6754 1 9649 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #0=(CFSignal (CObList) "attB2" 21 0 1 1065 1085 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1038..1058)") 
COMMENT        #1=(CFSignal (CObList) "tdTomato" 4 0 0 1118 2545 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1091..2518") 
COMMENT        #2=(CFSignal (CObList) "V5 epitope" 4 0 0 2564 2605 0 (CStringList) 
COMMENT            (CStringList "/invitrogen=\"1350000\"") 1 1 1 1 "2537..2578") 
COMMENT        #3=(CFSignal (CObList) "SV40 early promoter" 21 0 0 2660 2969 0 
COMMENT            (CStringList) (CStringList "/invitrogen=\"2290000\"") 1 1 1 1 
COMMENT            "2633..2942") 
COMMENT        #4=(CFSignal (CObList) "Puro(R)" 4 0 0 3029 3631 0 (CStringList) 
COMMENT            (CStringList "/invitrogen=\"1050000\"") 1 1 1 1 "3002..3604") 
COMMENT        #5=(CFSignal (CObList) "deltaU3" 19 0 0 3722 3775 0 (CStringList) 
COMMENT            (CStringList "/invitrogen=\"1410000\"") 1 1 1 1 "3695..3748") 
COMMENT        #6=(CFSignal (CObList) "deltaU3/3' LTR" 19 0 0 3723 3956 0 (CStringList) 
COMMENT            (CStringList "/invitrogen=\"1390000\"") 1 1 1 1 "3696..3929") 
COMMENT        #7=(CFSignal (CObList) "HIV-1 3' LTR" 19 0 0 3776 3956 0 (CStringList) 
COMMENT            (CStringList "/invitrogen=\"1430000\"") 1 1 1 1 "3749..3929") 
COMMENT        #8=(CFSignal (CObList) "SV40 pA" 21 0 0 4028 4158 0 (CStringList) 
COMMENT            (CStringList "/invitrogen=\"1840000\"") 1 1 1 1 "4001..4131") 
COMMENT        #9=(CFSignal (CObList) "f1 origin" 21 0 0 4531 4986 0 (CStringList) 
COMMENT            (CStringList "/invitrogen=\"2520000\"") 1 1 1 1 "4504..4959") 
COMMENT        #10=(CFSignal (CObList) "Amp(R)" 4 0 0 5117 5977 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1020000\"") 1 1 1 1 "5090..5950") 
COMMENT        #11=(CFSignal (CObList) "pUC origin" 21 0 0 6122 6795 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2540000\"") 1 1 1 1 "6095..6768") 
COMMENT        #12=(CFSignal (CObList) "RSV promoter" 21 0 0 7203 7431 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2270000\"") 1 1 1 1 "7176..7404") 
COMMENT        #13=(CFSignal (CObList) "RSV/5' LTR hybrid promoter" 21 0 0 7203 7612 0 
COMMENT             (CStringList) (CStringList "/invitrogen=\"2280000\"") 1 1 1 1 
COMMENT             "7176..7585") 
COMMENT        #14=(CFSignal (CObList) "HIV-1 5' LTR" 19 0 0 7432 7612 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1430000\"") 1 1 1 1 "7405..7585") 
COMMENT        #15=(CFSignal (CObList) "HIV-1 psi packaging signal" 21 0 0 7723 7767 0 
COMMENT             (CStringList) (CStringList "/invitrogen=\"1780000\"") 1 1 1 1 
COMMENT             "7696..7740") 
COMMENT        #16=(CFSignal (CObList) "HIV-1 Rev response element" 21 0 0 8277 8510 0 
COMMENT             (CStringList) (CStringList "/invitrogen=\"2440000\"") 1 1 1 1 
COMMENT             "8250..8483") 
COMMENT        #17=(CFSignal (CObList) "CMV promoter" 21 0 0 9011 9593 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2170000\"") 1 1 1 1 "8984..9566") 
COMMENT        #18=(CFSignal (CObList) "TATA box" 21 0 0 9511 9514 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"123000002\"") 1 1 1 1 "9484..9487") 
COMMENT        #19=(CFSignal (CObList) "mBeclin-C- Frag_122  - stop" 4 0 0 65 1045 0 
COMMENT             (CStringList) 
COMMENT             (CStringList "/origin=\"mBeclin + stop + primers (1.384bp linear)" 
COMMENT              "Fragment: from EcoRI cut at 11^12 to XhoI cut at" 
COMMENT              "1369^1370, 1.358bp           Orientation: original" 
COMMENT              "Plus strand 5':   no change           Plus strand 3':   no" 
COMMENT              "change           Minus strand 5':  no change" 
COMMENT              "Minus strand 3':  no change\"") 1 1 1 1 "65..1018")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                    "pLenti6-mBeclin1-C-Frag-122-tdTomato-V5-puro" 2) 
COMMENT                   5 "" 0 4) 
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COMMENT                          "DNA 'pLenti6-mBeclin1-C-Frag-122-tdTomato-V5-puro'" 
COMMENT                          1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 8564." 
COMMENT                          1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 08/24/16 02:32" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 08/24/16 02:32" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9649 bp" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Division: SYN" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 09-FEB-2012" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/pENTR3C\">pENTR3C</a>" 
COMMENT                          1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Sequence Source: Unknown (unknown)" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/Unknown\">Unknown(Unknown)</a>" 
COMMENT                          1) 
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COMMENT                          "Taxonomy: Unknown Unclassified" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #39=(CFolderPar 
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COMMENT                   "Annotations") 
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COMMENT                    "<a href=\"ivgnlink:/iq\">Invitrogen products related to pLenti6-mBeclin1-C-Frag-122-tdTomato-V5-puro</a>" 
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COMMENT                                        "   Plus strand 5':   no change           Plus strand 3':   no" 
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COMMENT                                        "   change           Minus strand 5':  no change" 
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COMMENT                                 "mBeclin-C- Frag_122  - stop") 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Amp(R)" "@N" 1 
COMMENT                                   -8.16357 -10.454 0 -10 3.14849 1.16519 #251#) 
COMMENT                             #253=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #19# 0) 1 2 0 0 #182# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    5.59379 6.23158 1 0.082322) 0.8 1.8 0) 
COMMENT                             #254=(CLabel (CWidget 0 (0 0) 1 2 0 0 #222# 1 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "mBeclin-C- Frag 122  - stop" "@N" 1 12.4584 
COMMENT                                   10.454 0 -10 11.0073 1.16519 #253#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #255=(CGroupWidget 
COMMENT                            (CWidget 19 (7 19 0) 1 2 0 0 Nil -538 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (CWidget 0 (3 #5# 0) 1 2 0 0 #187# 1378 100) 
COMMENT                                   (LOGPEN 0 8 8388608) 10 1 3.85402 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #257=(CLabel (CWidget 0 (0 0) 1 2 0 0 #179# 0 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "deltaU3" "@N" 1 
COMMENT                                   10.3308 -8.71165 0 -10 3.14849 1.06602 #256#) 
COMMENT                             #258=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #6# 0) 1 2 0 0 #194# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 
COMMENT                                    0.835356 3.70124 3.85337 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
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COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "deltaU3/3' LTR" 
COMMENT                                   "@N" 1 10.7909 -10.454 0 -10 5.97469 1.06602 
COMMENT                                   #258#) 
COMMENT                             #260=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #194# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 
COMMENT                                    0.670712 3.70124 3.81891 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #261=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #179# -858993459 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "HIV-1 3' LTR" 
COMMENT                                   "@N" 1 10.323 -12.1963 0 -10 5.05742 1.06602 
COMMENT                                   #260#) 
COMMENT                             #262=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #194# 813 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    1.32433 1.44201 1 0.082322) 0.8 1.8 0) 
COMMENT                             #263=(CLabel (CWidget 0 (0 0) 1 2 0 0 #179# 0 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "HIV-1 5' LTR" 
COMMENT                                   "@N" 1 -14.4678 3.48466 0 -10 5.05742 1.06602 
COMMENT                                   #262#)) (CObjectList)) 
COMMENT                      #264=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -257 100) 
COMMENT                            (CObjectList 
COMMENT                             #265=(CScratch 
COMMENT                                   (CWidget 0 (3 #0# 0) 1 2 0 0 #185# 1375 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 1 5.58144 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #266=(CLabel (CWidget 0 (0 0) 1 2 0 0 #223# 1 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "attB2" "@N" 1 
COMMENT                                   9.83426 8.71165 0 -10 2.15684 1.16519 #265#) 
COMMENT                             #267=(CLine 
COMMENT                                   (CWidget 0 (3 #3# 0) 1 2 0 0 #185# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 4.34292 
COMMENT                                   4.54447) 
COMMENT                             #268=(CLabel (CWidget 0 (0 0) 1 2 0 0 #223# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early promoter" "@N" 1 16.0777 -3.48466 
COMMENT                                   0 -10 8.35465 1.16519 #267#) 
COMMENT                             #269=(CLine 
COMMENT                                   (CWidget 0 (3 #8# 0) 1 2 0 0 #185# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 3.56991 
COMMENT                                   3.65508) 
COMMENT                             #270=(CLabel (CWidget 0 (0 0) 1 2 0 0 #223# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 pA" "@N" 1 
COMMENT                                   8.26633 -13.9386 0 -10 3.74348 1.16519 #269#) 
COMMENT                             #271=(CLine 
COMMENT                                   (CWidget 0 (3 #9# 0) 1 2 0 0 #185# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 3.0316 
COMMENT                                   3.32806) 
COMMENT                             #272=(CLabel (CWidget 0 (0 0) 1 2 0 0 #223# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "f1 origin" "@N" 
COMMENT                                   1 3.23631 -15.681 0 -10 3.32203 1.16519 #271#) 
COMMENT                             #273=(CLine 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #185# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 1.85549 
COMMENT                                   2.29369) 
COMMENT                             #274=(CLabel (CWidget 0 (0 0) 1 2 0 0 #223# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pUC origin" 
COMMENT                                   "@N" 1 -13.3003 -5.22699 0 -10 4.14014 
COMMENT                                   1.16519 #273#) 
COMMENT                             #275=(CLine 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #185# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 1.44201 
COMMENT                                   1.59089) 
COMMENT                             #276=(CLabel (CWidget 0 (0 0) 1 2 0 0 #223# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "RSV promoter" 
COMMENT                                   "@N" 1 -15.0161 0 0 -10 5.72678 1.16519 #275#) 
COMMENT                             #277=(CLine 
COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #185# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.670712 1.32433 
COMMENT                                   1.59089) 
COMMENT                             #278=(CLabel (CWidget 0 (0 0) 1 2 0 0 #223# 1 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "RSV/5' LTR hybrid promoter" "@N" 1 -17.9266 
COMMENT                                   1.74233 0 -10 11.1809 1.16519 #277#) 
COMMENT                             #279=(CScratch 
COMMENT                                   (CWidget 0 (3 #15# 0) 1 2 0 0 #185# 1375 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 1 1.25282 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #280=(CLabel (CWidget 0 (0 0) 1 2 0 0 #223# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HIV-1 psi packaging signal" "@N" 1 -17.2984 
COMMENT                                   5.22699 0 -10 10.5859 1.16519 #279#) 
COMMENT                             #281=(CLine 
COMMENT                                   (CWidget 0 (3 #16# 0) 1 2 0 0 #185# 695412850 
COMMENT                                    100) (LOGPEN 0 6 16711680) 8 0.835356 
COMMENT                                   0.740508 0.89264) 
COMMENT                             #282=(CLabel (CWidget 0 (0 0) 1 2 0 0 #223# 1 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HIV-1 Rev response element" "@N" 1 -15.2844 
COMMENT                                   8.71165 0 -10 11.5031 1.16519 #281#) 
COMMENT                             #283=(CLine 
COMMENT                                   (CWidget 0 (3 #17# 0) 1 2 0 0 #185# 695412850 
COMMENT                                    100) (LOGPEN 0 6 16711680) 8 0.835356 
COMMENT                                   0.0364077 0.415438) 
COMMENT                             #284=(CLabel (CWidget 0 (0 0) 1 2 0 0 #223# 1 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "CMV promoter" 
COMMENT                                   "@N" 1 -9.6097 10.454 0 -10 5.80115 1.16519 
COMMENT                                   #283#) 
COMMENT                             #285=(CScratch 
COMMENT                                   (CWidget 0 (3 #18# 0) 1 2 0 0 #185# 1378 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 1 0.0903692 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #286=(CLabel (CWidget 0 (0 0) 1 2 0 0 #223# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "TATA box" "@N" 
COMMENT                                   1 -3.9375 12.1963 0 -10 4.14014 1.16519 #285#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #287=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -608 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #288=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -819 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #289=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1241 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) -2147455383 
COMMENT       (CStringList) 2147483678 268632118 (CObList)))
FEATURES             Location/Qualifiers
     Source          join(<1022..>1044,<1..>56)
                     /invitrogen="563"
                     /vntifkey="21"
                     /label=Source_1
                     /note="Unknown feature type:Source"
     Misc._recombina join(9616..9622,1..14)
                     /vntifkey="21"
                     /label=attB1
                     /note="Unknown feature type:Misc._recombina"
     Vector          join(1022..>1044,<1..56)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="Unknown feature type:Vector"
     CDS             65..1045
                     /origin="mBeclin + stop + primers (1.384bp linear)
                     Fragment: from EcoRI cut at 11^12 to XhoI cut at
                     1369^1370, 1.358bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /vntifkey="4"
                     /label=mBeclin-C-\Frag_122\\-\stop
     Misc._recombina complement(1065..1085)
                     /vntifkey="21"
                     /label=attB2
                     /note="Unknown feature type:Misc._recombina"
     CDS             1118..2545
                     /vntifkey="4"
                     /label=tdTomato
                     /note="Oriinal location: 4176..5603"
     CDS             2564..2605
                     /invitrogen="1350000"
                     /vntifkey="4"
                     /label=V5\epitope
                     /note="Oriinal location: 5622..5663"
     Promoter        2660..2969
                     /invitrogen="2290000"
                     /vntifkey="21"
                     /label=SV40\early\promoter
                     /note="Unknown feature type:Promoter  Oriinal location: 5718..6027"
     CDS             3029..3631
                     /invitrogen="1050000"
                     /vntifkey="4"
                     /label=Puro(R)
                     /note="Oriinal location: 6087..6689"
     LTR             3722..3775
                     /invitrogen="1410000"
                     /vntifkey="19"
                     /label=deltaU3
                     /note="Oriinal location: 6780..6833"
     LTR             3723..3956
                     /invitrogen="1390000"
                     /vntifkey="19"
                     /label=deltaU3/3'\LTR
                     /note="Oriinal location: 6781..7014"
     LTR             3776..3956
                     /invitrogen="1430000"
                     /vntifkey="19"
                     /label=HIV-1\3'\LTR
                     /note="Oriinal location: 6834..7014"
     PolyA_signal    4028..4158
                     /invitrogen="1840000"
                     /vntifkey="21"
                     /label=SV40\pA
                     /note="Unknown feature type:PolyA_signal  Oriinal location: 7086..7216"
     Replication_ori 4531..4986
                     /invitrogen="2520000"
                     /vntifkey="21"
                     /label=f1\origin
                     /note="Unknown feature type:Replication_ori  Oriinal location: 7589..8044"
     CDS             5117..5977
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
                     /note="Oriinal location: 8175..9035"
     Replication_ori 6122..6795
                     /invitrogen="2540000"
                     /vntifkey="21"
                     /label=pUC\origin
                     /note="Unknown feature type:Replication_ori  Oriinal location: 9180..9853"
     Promoter        7203..7431
                     /invitrogen="2270000"
                     /vntifkey="21"
                     /label=RSV\promoter
                     /note="Unknown feature type:Promoter  Oriinal location: 1..229"
     Promoter        7203..7612
                     /invitrogen="2280000"
                     /vntifkey="21"
                     /label=RSV/5'\LTR\hybrid\promoter
                     /note="Unknown feature type:Promoter  Oriinal location: 1..410"
     LTR             7432..7612
                     /invitrogen="1430000"
                     /vntifkey="19"
                     /label=HIV-1\5'\LTR
                     /note="Oriinal location: 230..410"
     Misc._binding   7723..7767
                     /invitrogen="1780000"
                     /vntifkey="21"
                     /label=HIV-1\psi\packaging\signal
                     /note="Unknown feature type:Misc._binding  Oriinal location: 521..565"
     Protein_binding 8277..8510
                     /invitrogen="2440000"
                     /vntifkey="21"
                     /label=HIV-1\Rev\response\element
                     /note="Unknown feature type:Protein_binding  Oriinal location: 1075..1308"
     Promoter        9011..9593
                     /invitrogen="2170000"
                     /vntifkey="21"
                     /label=CMV\promoter
                     /note="Unknown feature type:Promoter  Oriinal location: 1809..2391"
     TATA_box        9511..9514
                     /invitrogen="123000002"
                     /vntifkey="21"
                     /label=TATA\box
                     /note="Unknown feature type:TATA_box  Oriinal location: 2309..2312"
BASE COUNT     2442 a      2476 c      2583 g      2148 t 
ORIGIN
        1 gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg actggatccg gtaccgaatt 
       61 caccatgatc atgtcgggcc agaccgatgt ggatcacccg ctgtgtgagg aatgcacaga 
      121 cactctttta gaccagctgg acactcagct caatgtcact gagaatgaat gtcagaacta 
      181 caaacgctgt ttggagatcc tagagcagat gaatgaggat gacagtgagc agctgcagag 
      241 ggagctgaag gagctggcct tggaggagga gaggctgatc caggagctgg aagatgtgga 
      301 aaagaaccgc aaggtggtgg cagagaacct ggagaaggtc caggctgagg cggagagatt 
      361 ggaccaggag gaagctcagt accagcggga gtatagtgag tttaaaaggc agcagctgga 
      421 gttggatgac gaactcaaga gtgtggagaa ccaggtgcgc tacgcccaga tccagctgga 
      481 caagctcaag aaaaccaatg tcttcaatgc caccttccac atctggcaca gcggacagtt 
      541 tggcacaatc aataatttca gactgggtcg cttgcccagt gttcctgtgg agtggaatga 
      601 aatcaatgct gcctggggcc agacagtgct gctgctccat gctttggcca ataagatggg 
      661 tctgaagttt cagaggtacc gacttgttcc ctatggaaat cattcctatc tggagtctct 
      721 gacagacaaa tctaaggagt tgccgttata ctgttctggg ggtttgcggt ttttctggga 
      781 caacaagttt gaccatgcaa tggtagcttt tctggactgt gtgcagcagt tcaaagaaga 
      841 ggtggaaaaa ggagagactc gattttgtct tccgtacagg atggacgtgg agaaaggcaa 
      901 gattgaagac actggaggca gtggcggctc ctattccatc aaaacccagt ttaactcgga 
      961 ggagcagtgg acaaaagcgc tcaagttcat gctgaccaat ctcaagtggg gtcttgcctg 
     1021 ggtgtcctca cagttctata acaagcactc gagatatcta gacccagctt tcttgtacaa 
     1081 agtggttgat atccagcaca gtggcggccg ctcgagtatg gtgagcaagg gcgaggaggt 
     1141 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt 
     1201 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa 
     1261 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta 
     1321 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt 
     1381 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt 
     1441 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac 
     1501 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac 
     1561 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct 
     1621 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt 
     1681 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga 
     1741 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca 
     1801 tggcaccggc agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat 
     1861 ggccgtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca 
     1921 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa 
     1981 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt 
     2041 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct 
     2101 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt 
     2161 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg 
     2221 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc 
     2281 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct 
     2341 gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa 
     2401 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa 
     2461 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct 
     2521 gtacggcatg gacgagctgt acaaggaggg cccgcggttc gaaggtaagc ctatccctaa 
     2581 ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa tgagtttgga attaattctg 
     2641 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat 
     2701 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 
     2761 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 
     2821 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 
     2881 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 
     2941 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 
     3001 cattttcgga tctgatcagc acgtgaccat ggccaccgag tacaagccca cggtgcgcct 
     3061 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta 
     3121 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca 
     3181 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 
     3241 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 
     3301 gatcggctcg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 
     3361 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 
     3421 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 
     3481 ggccgagcgc gctggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 
     3541 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 
     3601 ctggtgcatg acccgcaagc ccggtgcctg agctaagcac aattcgagct cggtaccttt 
     3661 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 
     3721 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc 
     3781 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
     3841 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
     3901 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 
     3961 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 
     4021 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     4081 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     4141 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta 
     4201 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 
     4261 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 
     4321 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg 
     4381 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 
     4441 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 
     4501 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg 
     4561 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 
     4621 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 
     4681 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 
     4741 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 
     4801 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 
     4861 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 
     4921 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta 
     4981 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 
     5041 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 
     5101 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 
     5161 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 
     5221 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 
     5281 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 
     5341 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 
     5401 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 
     5461 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 
     5521 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 
     5581 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 
     5641 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 
     5701 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 
     5761 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 
     5821 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 
     5881 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 
     5941 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 
     6001 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     6061 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     6121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     6181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     6241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 
     6301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     6361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     6421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     6481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     6541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     6601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     6661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     6721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     6781 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 
     6841 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 
     6901 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 
     6961 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 
     7021 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 
     7081 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca 
     7141 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc 
     7201 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa 
     7261 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac 
     7321 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa 
     7381 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc 
     7441 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 
     7501 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 
     7561 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc 
     7621 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc 
     7681 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt 
     7741 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga 
     7801 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat 
     7861 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt 
     7921 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag 
     7981 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa 
     8041 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa 
     8101 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg 
     8161 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 
     8221 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 
     8281 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 
     8341 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 
     8401 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 
     8461 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 
     8521 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 
     8581 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 
     8641 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 
     8701 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 
     8761 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 
     8821 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 
     8881 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 
     8941 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 
     9001 taagcttggg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc 
     9061 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg 
     9121 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat 
     9181 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc 
     9241 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta 
     9301 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag 
     9361 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt 
     9421 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa 
     9481 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt 
     9541 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgactctag 
     9601 aggatccact agtccagtgt ggtggaattc tgcagatatc aacaagttt 
//
