LOCUS pLenti6-mBeclin1-C-Frag-147-puro 8128 bp DNA circular SYN 09-FEB-2012 DEFINITION LMBP 8578. SOURCE ORGANISM COMMENT Accession number: LMBP 8578 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) DBSOURCE GenBank Unknown Unclassified FEATURES Location/Qualifiers Source join(<977..>999,<1..>56) /invitrogen="563" /label=Source_1 Misc._recombina join(8122..8128,1..14) /label=attB1 Vector join(977..>999,<1..56) /origin="pENTR3C (2.723bp circular) Target vector: from XhoI cut at 938^939 to EcoRI cut at 496^497, 2.281bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" CDS 65..973 /origin="mBeclin + stop + primers (1.384bp linear) Fragment: from EcoRI cut at 11^12 to XhoI cut at 1369^1370, 1.358bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label="mBeclin-C- Frag_147 + stop" Misc._recombina complement(993..1013) /label=attB2 Misc._feature 1057..1081 /label="FLAG tag" CDS 1094..1102 /invitrogen="123000001" /note="Oriinal location: 8422..8430" /label="3 stops" Promoter 1139..1448 /invitrogen="2290000" /note="Oriinal location: 8467..8776" /label="SV40 early promoter" CDS 1508..2110 /invitrogen="1050000" /note="Oriinal location: 1..603" /label=Puro(R) LTR 2201..2254 /invitrogen="1410000" /note="Oriinal location: 694..747" /label=deltaU3 LTR 2202..2435 /invitrogen="1390000" /note="Oriinal location: 695..928" /label="deltaU3/3' LTR" LTR 2255..2435 /invitrogen="1430000" /note="Oriinal location: 748..928" /label="HIV-1 3' LTR" PolyA_signal 2507..2637 /invitrogen="1840000" /note="Oriinal location: 1000..1130" /label="SV40 pA" Replication_ori 3010..3465 /invitrogen="2520000" /note="Oriinal location: 1503..1958" /label="f1 origin" Promoter 3497..3595 /invitrogen="2330000" /note="Oriinal location: 1990..2088" /label="bla promoter" CDS 3596..4456 /invitrogen="1020000" /note="Oriinal location: 2089..2949" /label=Amp(R) Replication_ori 4601..5274 /invitrogen="2540000" /note="Oriinal location: 3094..3767" /label="pUC origin" Source 5681^5682 /invitrogen="765" /note="Oriinal location: 4175..4174" /label=Source_1 Promoter 5682..5910 /invitrogen="2270000" /note="Oriinal location: 4175..4403" /label="RSV promoter" Promoter 5682..6091 /invitrogen="2280000" /note="Oriinal location: 4175..4584" /label="RSV/5' LTR hybrid promoter" LTR 5911..6091 /invitrogen="1430000" /note="Oriinal location: 4404..4584" /label="HIV-1 5' LTR" Misc._feature 6201 /invitrogen="123000012" /note="Oriinal location: 4694..4694" /label="splice donor" Misc._binding 6202..6246 /invitrogen="1780000" /note="Oriinal location: 4695..4739" /label="HIV-1 psi packaging signal" Protein_binding 6756..6989 /invitrogen="2440000" /note="Oriinal location: 5249..5482" /label="HIV-1 Rev response element" Misc._feature 7337 /invitrogen="123000013" /note="Oriinal location: 5830..5830" /label="splice acceptor" Misc._feature 7365 /invitrogen="123000014" /note="Oriinal location: 5858..5858" /label="splice acceptor" Promoter 7490..8072 /invitrogen="2170000" /note="Oriinal location: 5983..6565" /label="CMV promoter" TATA_box 7990..7993 /invitrogen="123000002" /note="Oriinal location: 6483..6486" /label="TATA box" ORIGIN 1 GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG ACTGGATCCG GTACCGAATT 61 CACCATGACT CAGCTCAATG TCACTGAGAA TGAATGTCAG AACTACAAAC GCTGTTTGGA 121 GATCCTAGAG CAGATGAATG AGGATGACAG TGAGCAGCTG CAGAGGGAGC TGAAGGAGCT 181 GGCCTTGGAG GAGGAGAGGC TGATCCAGGA GCTGGAAGAT GTGGAAAAGA ACCGCAAGGT 241 GGTGGCAGAG AACCTGGAGA AGGTCCAGGC TGAGGCGGAG AGATTGGACC AGGAGGAAGC 301 TCAGTACCAG CGGGAGTATA GTGAGTTTAA AAGGCAGCAG CTGGAGTTGG ATGACGAACT 361 CAAGAGTGTG GAGAACCAGG TGCGCTACGC CCAGATCCAG CTGGACAAGC TCAAGAAAAC 421 CAATGTCTTC AATGCCACCT TCCACATCTG GCACAGCGGA CAGTTTGGCA CAATCAATAA 481 TTTCAGACTG GGTCGCTTGC CCAGTGTTCC TGTGGAGTGG AATGAAATCA ATGCTGCCTG 541 GGGCCAGACA GTGCTGCTGC TCCATGCTTT GGCCAATAAG ATGGGTCTGA AGTTTCAGAG 601 GTACCGACTT GTTCCCTATG GAAATCATTC CTATCTGGAG TCTCTGACAG ACAAATCTAA 661 GGAGTTGCCG TTATACTGTT CTGGGGGTTT GCGGTTTTTC TGGGACAACA AGTTTGACCA 721 TGCAATGGTA GCTTTTCTGG ACTGTGTGCA GCAGTTCAAA GAAGAGGTGG AAAAAGGAGA 781 GACTCGATTT TGTCTTCCGT ACAGGATGGA CGTGGAGAAA GGCAAGATTG AAGACACTGG 841 AGGCAGTGGC GGCTCCTATT CCATCAAAAC CCAGTTTAAC TCGGAGGAGC AGTGGACAAA 901 AGCGCTCAAG TTCATGCTGA CCAATCTCAA GTGGGGTCTT GCCTGGGTGT CCTCACAGTT 961 CTATAACAAG TGACACTCGA GATATCTAGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT 1021 CCAGCACAGT GGCGGCCGCT CGAGTCTAGA GGGCCCGGAC TACAAGGATG ACGATGACAA 1081 AACGCGTACC GGTTAGTAAT GAGTTTGGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG 1141 TGTGGAAAGT CCCCAGGCTC CCCAGGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA 1201 GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT 1261 GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC 1321 TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA 1381 GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG 1441 CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAGCA 1501 CGTGACCATG GCCACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC 1561 CCGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT 1621 CGACCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT 1681 CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC 1741 CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCTCGC GCATGGCCGA 1801 GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG 1861 GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA 1921 GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CTGGGGTGCC 1981 CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC 2041 CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC 2101 CGGTGCCTGA GCTAAGCACA ATTCGAGCTC GGTACCTTTA AGACCAATGA CTTACAAGGC 2161 AGCTGTAGAT CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC 2221 CCAACGAAGA CAAGATCTGC TTTTTGCTTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG 2281 AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC 2341 TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT 2401 CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTAGT AGTTCATGTC ATCTTATTAT 2461 TCAGTATTTA TAACTTGCAA AGAAATGAAT ATCAGAGAGT GAGAGGAACT TGTTTATTGC 2521 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT 2581 TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGCT 2641 CTAGCTATCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 2701 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 2761 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGGAC GTACCCAATT 2821 CGCCCTATAG TGAGTCGTAT TACGCGCGCT CACTGGCCGT CGTTTTACAA CGTCGTGACT 2881 GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT 2941 GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG 3001 GCGAATGGGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 3061 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 3121 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 3181 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 3241 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 3301 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 3361 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 3421 AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGCTTAC AATTTAGGTG GCACTTTTCG 3481 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 3541 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 3601 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT 3661 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 3721 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA 3781 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 3841 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 3901 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG 3961 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG 4021 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG 4081 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 4141 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG 4201 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC 4261 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 4321 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC 4381 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 4441 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA 4501 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA 4561 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 4621 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 4681 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 4741 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA 4801 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 4861 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 4921 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 4981 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC 5041 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 5101 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 5161 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 5221 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT 5281 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC 5341 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG 5401 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA 5461 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC 5521 TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT 5581 GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCGCGCAAT 5641 TAACCCTCAC TAAAGGGAAC AAAAGCTGGA GCTGCAAGCT TAATGTAGTC TTATGCAATA 5701 CTCTTGTAGT CTTGCAACAT GGTAACGATG AGTTAGCAAC ATGCCTTACA AGGAGAGAAA 5761 AAGCACCGTG CATGCCGATT GGTGGAAGTA AGGTGGTACG ATCGTGCCTT ATTAGGAAGG 5821 CAACAGACGG GTCTGACATG GATTGGACGA ACCACTGAAT TGCCGCATTG CAGAGATATT 5881 GTATTTAAGT GCCTAGCTCG ATACATAAAC GGGTCTCTCT GGTTAGACCA GATCTGAGCC 5941 TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA 6001 GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA 6061 CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA 6121 AAGGGAAACC AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA 6181 GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG 6241 AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA 6301 AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA 6361 GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA 6421 GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT 6481 TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA 6541 AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG 6601 CGGCCGCTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA 6661 TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA 6721 AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG 6781 GGAGCAGCAG GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA GGCCAGACAA 6841 TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG 6901 CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG 6961 GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT 7021 TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTTGG 7081 AATCACACGA CCTGGATGGA GTGGGACAGA GAAATTAACA ATTACACAAG CTTAATACAC 7141 TCCTTAATTG AAGAATCGCA AAACCAGCAA GAAAAGAATG AACAAGAATT ATTGGAATTA 7201 GATAAATGGG CAAGTTTGTG GAATTGGTTT AACATAACAA ATTGGCTGTG GTATATAAAA 7261 TTATTCATAA TGATAGTAGG AGGCTTGGTA GGTTTAAGAA TAGTTTTTGC TGTACTTTCT 7321 ATAGTGAATA GAGTTAGGCA GGGATATTCA CCATTATCGT TTCAGACCCA CCTCCCAACC 7381 CCGAGGGGAC CCGACAGGCC CGAAGGAATA GAAGAAGAAG GTGGAGAGAG AGACAGAGAC 7441 AGATCCATTC GATTAGTGAA CGGATCTCGA CGGTATCGAT AAGCTTGGGA GTTCCGCGTT 7501 ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG 7561 TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG 7621 GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT 7681 ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG 7741 ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG 7801 GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT 7861 CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC 7921 TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG 7981 TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT 8041 CCACGCTGTT TTGACCTCCA TAGAAGACAC CGACTCTAGA GGATCCACTA GTCCAGTGTG 8101 GTGGAATTCT GCAGATATCA ACAAGTTT //