LOCUS       pLenti6-mBeclin1-N-Frag-122-Flag-puro      7585 bp    DNA     circular SYN 09-FEB-2012
DEFINITION  LMBP 8594.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8594
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
DBSOURCE    GenBank
            Unknown
            Unclassified
FEATURES             Location/Qualifiers
     Source          join(<434..>456,<1..>56)
                     /invitrogen="563"
                     /label=Source_1
     Misc._recombina join(7579..7585,1..14)
                     /label=attB1
     Vector          join(434..>456,<1..56)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
     CDS             65..430
                     /origin="mBeclin + stop + primers (1.384bp linear)
                     Fragment: from EcoRI cut at 11^12 to XhoI cut at
                     1369^1370, 1.358bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /label="Beclin1_N-term_122 - stop"
     Misc._recombina complement(450..470)
                     /label=attB2
     Misc._feature   514..538
                     /label="FLAG tag"
     CDS             551..559
                     /invitrogen="123000001"
                     /note="Oriinal location: 8422..8430"
                     /label="3 stops"
     Promoter        596..905
                     /invitrogen="2290000"
                     /note="Oriinal location: 8467..8776"
                     /label="SV40 early promoter"
     CDS             965..1567
                     /invitrogen="1050000"
                     /note="Oriinal location: 1..603"
                     /label=Puro(R)
     LTR             1658..1711
                     /invitrogen="1410000"
                     /note="Oriinal location: 694..747"
                     /label=deltaU3
     LTR             1659..1892
                     /invitrogen="1390000"
                     /note="Oriinal location: 695..928"
                     /label="deltaU3/3' LTR"
     LTR             1712..1892
                     /invitrogen="1430000"
                     /note="Oriinal location: 748..928"
                     /label="HIV-1 3' LTR"
     PolyA_signal    1964..2094
                     /invitrogen="1840000"
                     /note="Oriinal location: 1000..1130"
                     /label="SV40 pA"
     Replication_ori 2467..2922
                     /invitrogen="2520000"
                     /note="Oriinal location: 1503..1958"
                     /label="f1 origin"
     Promoter        2954..3052
                     /invitrogen="2330000"
                     /note="Oriinal location: 1990..2088"
                     /label="bla promoter"
     CDS             3053..3913
                     /invitrogen="1020000"
                     /note="Oriinal location: 2089..2949"
                     /label=Amp(R)
     Replication_ori 4058..4731
                     /invitrogen="2540000"
                     /note="Oriinal location: 3094..3767"
                     /label="pUC origin"
     Source          5138^5139
                     /invitrogen="765"
                     /note="Oriinal location: 4175..4174"
                     /label=Source_1
     Promoter        5139..5367
                     /invitrogen="2270000"
                     /note="Oriinal location: 4175..4403"
                     /label="RSV promoter"
     Promoter        5139..5548
                     /invitrogen="2280000"
                     /note="Oriinal location: 4175..4584"
                     /label="RSV/5' LTR hybrid promoter"
     LTR             5368..5548
                     /invitrogen="1430000"
                     /note="Oriinal location: 4404..4584"
                     /label="HIV-1 5' LTR"
     Misc._feature   5658
                     /invitrogen="123000012"
                     /note="Oriinal location: 4694..4694"
                     /label="splice donor"
     Misc._binding   5659..5703
                     /invitrogen="1780000"
                     /note="Oriinal location: 4695..4739"
                     /label="HIV-1 psi packaging signal"
     Protein_binding 6213..6446
                     /invitrogen="2440000"
                     /note="Oriinal location: 5249..5482"
                     /label="HIV-1 Rev response element"
     Misc._feature   6794
                     /invitrogen="123000013"
                     /note="Oriinal location: 5830..5830"
                     /label="splice acceptor"
     Misc._feature   6822
                     /invitrogen="123000014"
                     /note="Oriinal location: 5858..5858"
                     /label="splice acceptor"
     Promoter        6947..7529
                     /invitrogen="2170000"
                     /note="Oriinal location: 5983..6565"
                     /label="CMV promoter"
     TATA_box        7447..7450
                     /invitrogen="123000002"
                     /note="Oriinal location: 6483..6486"
                     /label="TATA box"
ORIGIN
        1 GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG ACTGGATCCG GTACCGAATT
       61 CACCATGGAG GGGTCTAAGG CGTCCAGCAG CACCATGCAG GTGAGCTTCG TGTGCCAGCG
      121 CTGTAGCCAG CCTCTGAAAC TGGACACGAG CTTCAAGATC CTGGACCGGG TCACCATCCA
      181 GGAACTCACA GCTCCATTAC TTACCACAGC CCAGGCGAAA CCAGGAGAGA CCCAGGAGGA
      241 AGAGGCTAAC TCAGGAGAGG AGCCATTTAT TGAAACTCGC CAGGATGGTG TCTCTCGAAG
      301 ATTCATCCCC CCAGCCAGGA TGATGTCTAC AGAAAGTGCT AATAGCTTCA CTCTGATCGG
      361 GGAGGCATCT GATGGTGGCA CCATGGAGAA CCTCAGCCGG AGACTCAAGG TCACTGGAGA
      421 CCTGTTTGAC CACTCGAGAT ATCTAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCCA
      481 GCACAGTGGC GGCCGCTCGA GTCTAGAGGG CCCGGACTAC AAGGATGACG ATGACAAAAC
      541 GCGTACCGGT TAGTAATGAG TTTGGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT
      601 GGAAAGTCCC CAGGCTCCCC AGGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC
      661 AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA
      721 TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC
      781 GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC
      841 CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT
      901 AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT
      961 GACCATGGCC ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCCG
     1021 GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA
     1081 CCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG
     1141 GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC
     1201 GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCTCGCGCA TGGCCGAGTT
     1261 GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC
     1321 CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG
     1381 TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCTG GGGTGCCCGC
     1441 CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT
     1501 CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG
     1561 TGCCTGAGCT AAGCACAATT CGAGCTCGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC
     1621 TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA
     1681 ACGAAGACAA GATCTGCTTT TTGCTTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC
     1741 CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG
     1801 AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG
     1861 ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTAGTAGT TCATGTCATC TTATTATTCA
     1921 GTATTTATAA CTTGCAAAGA AATGAATATC AGAGAGTGAG AGGAACTTGT TTATTGCAGC
     1981 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
     2041 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGCTCTA
     2101 GCTATCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT
     2161 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT
     2221 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGGACGTA CCCAATTCGC
     2281 CCTATAGTGA GTCGTATTAC GCGCGCTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG
     2341 AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC
     2401 GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG
     2461 AATGGGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG
     2521 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC
     2581 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC
     2641 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA
     2701 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA
     2761 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG
     2821 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA
     2881 AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTAGGTGGCA CTTTTCGGGG
     2941 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
     3001 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT
     3061 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
     3121 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG
     3181 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG
     3241 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA
     3301 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA
     3361 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC
     3421 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC
     3481 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG
     3541 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC
     3601 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA
     3661 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT
     3721 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT
     3781 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG
     3841 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT
     3901 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT
     3961 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT
     4021 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
     4081 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT
     4141 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG
     4201 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA
     4261 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC
     4321 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
     4381 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC
     4441 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA
     4501 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG
     4561 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG
     4621 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
     4681 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC
     4741 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC
     4801 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC
     4861 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG
     4921 GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA
     4981 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG
     5041 CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC GCGCAATTAA
     5101 CCCTCACTAA AGGGAACAAA AGCTGGAGCT GCAAGCTTAA TGTAGTCTTA TGCAATACTC
     5161 TTGTAGTCTT GCAACATGGT AACGATGAGT TAGCAACATG CCTTACAAGG AGAGAAAAAG
     5221 CACCGTGCAT GCCGATTGGT GGAAGTAAGG TGGTACGATC GTGCCTTATT AGGAAGGCAA
     5281 CAGACGGGTC TGACATGGAT TGGACGAACC ACTGAATTGC CGCATTGCAG AGATATTGTA
     5341 TTTAAGTGCC TAGCTCGATA CATAAACGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG
     5401 GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG
     5461 CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC
     5521 TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG
     5581 GGAAACCAGA GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG
     5641 GCGAGGGGCG GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA
     5701 GAGATGGGTG CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA
     5761 TTCGGTTAAG GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA
     5821 GGGAGCTAGA ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC
     5881 AAATACTGGG ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT
     5941 ATAATACAGT AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG
     6001 AAGCTTTAGA CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGCGG
     6061 CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT
     6121 AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA
     6181 GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA
     6241 GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA
     6301 TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT
     6361 CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA
     6421 AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC
     6481 ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT
     6541 CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC
     6601 TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT
     6661 AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA
     6721 TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA
     6781 GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG
     6841 AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA
     6901 TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGATAAG CTTGGGAGTT CCGCGTTACA
     6961 TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA
     7021 ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG
     7081 GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG
     7141 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC
     7201 TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG
     7261 ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA
     7321 AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT
     7381 CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG
     7441 GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA
     7501 CGCTGTTTTG ACCTCCATAG AAGACACCGA CTCTAGAGGA TCCACTAGTC CAGTGTGGTG
     7561 GAATTCTGCA GATATCAACA AGTTT
//