LOCUS pLenti6-mBeclin1-N-Frag-131-puro 7615 bp DNA circular SYN 10-FEB-2012 DEFINITION LMBP 8595. SOURCE ORGANISM COMMENT Accession number: LMBP 8595 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) DBSOURCE GenBank FEATURES Location/Qualifiers Misc._recombina complement(join(7609..7615,1..14)) /label=attB2 Misc._feature 58..82 /label="FLAG tag" CDS 95..103 /invitrogen="123000001" /note="Oriinal location: 8422..8430" /label="3 stops" Promoter 140..449 /invitrogen="2290000" /note="Oriinal location: 8467..8776" /label="SV40 early promoter" CDS 509..1111 /invitrogen="1050000" /note="Oriinal location: 1..603" /label=Puro(R) LTR 1202..1255 /invitrogen="1410000" /note="Oriinal location: 694..747" /label=deltaU3 LTR 1203..1436 /invitrogen="1390000" /note="Oriinal location: 695..928" /label="deltaU3/3' LTR" LTR 1256..1436 /invitrogen="1430000" /note="Oriinal location: 748..928" /label="HIV-1 3' LTR" PolyA_signal 1508..1638 /invitrogen="1840000" /note="Oriinal location: 1000..1130" /label="SV40 pA" Replication_ori 2011..2466 /invitrogen="2520000" /note="Oriinal location: 1503..1958" /label="f1 origin" Promoter 2498..2596 /invitrogen="2330000" /note="Oriinal location: 1990..2088" /label="bla promoter" CDS 2597..3457 /invitrogen="1020000" /note="Oriinal location: 2089..2949" /label=Amp(R) Replication_ori 3602..4275 /invitrogen="2540000" /note="Oriinal location: 3094..3767" /label="pUC origin" Promoter 4683..4911 /invitrogen="2270000" /note="Oriinal location: 4175..4403" /label="RSV promoter" Promoter 4683..5092 /invitrogen="2280000" /note="Oriinal location: 4175..4584" /label="RSV/5' LTR hybrid promoter" Source 4682^4683 /invitrogen="765" /note="Oriinal location: 4175..4174" /label=Source_1 LTR 4912..5092 /invitrogen="1430000" /note="Oriinal location: 4404..4584" /label="HIV-1 5' LTR" Misc._feature 5202 /invitrogen="123000012" /note="Oriinal location: 4694..4694" /label="splice donor" Misc._binding 5203..5247 /invitrogen="1780000" /note="Oriinal location: 4695..4739" /label="HIV-1 psi packaging signal" Protein_binding 5757..5990 /invitrogen="2440000" /note="Oriinal location: 5249..5482" /label="HIV-1 Rev response element" Misc._feature 6338 /invitrogen="123000013" /note="Oriinal location: 5830..5830" /label="splice acceptor" Misc._feature 6366 /invitrogen="123000014" /note="Oriinal location: 5858..5858" /label="splice acceptor" Promoter 6491..7073 /invitrogen="2170000" /note="Oriinal location: 5983..6565" /label="CMV promoter" TATA_box 6991..6994 /invitrogen="123000002" /note="Oriinal location: 6483..6486" /label="TATA box" Misc._recombina 7123..7143 /label=attB1 Source join(<7593..>7615,<7130..>7185) /invitrogen="563" /label=Source_1 Vector join(7593..>7615,<7130..7185) /origin="pENTR3C (2.723bp circular) Target vector: from XhoI cut at 938^939 to EcoRI cut at 496^497, 2.281bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Restriction_sit join(7179..7184,7183^7184) /note="Name: KpnI" /note="Pattern: ggtacc" /note="Number_of_matches: 2" /note="cut_0_on_positive_strand: 494^495" /note="cut_0_on_negative_strand: 490^491" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI CDS 7194..7589 /origin="mBeclin + stop + primers (1.384bp linear) Fragment: from EcoRI cut at 11^12 to XhoI cut at 1369^1370, 1.358bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=mBeclin1-N-term_131_nostop ORIGIN 1 CTTGTACAAA GTGGTTGATA TCCAGCACAG TGGCGGCCGC TCGAGTCTAG AGGGCCCGGA 61 CTACAAGGAT GACGATGACA AAACGCGTAC CGGTTAGTAA TGAGTTTGGA ATTAATTCTG 121 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGGCAG GCAGAAGTAT 181 GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC 241 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC 301 TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT 361 AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA 421 GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC 481 CATTTTCGGA TCTGATCAGC ACGTGACCAT GGCCACCGAG TACAAGCCCA CGGTGCGCCT 541 CGCCACCCGC GACGACGTCC CCCGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA 601 CCCCGCCACG CGCCACACCG TCGACCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA 661 AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG 721 CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA 781 GATCGGCTCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA 841 AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT 901 CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC 961 GGCCGAGCGC GCTGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT 1021 CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC 1081 CTGGTGCATG ACCCGCAAGC CCGGTGCCTG AGCTAAGCAC AATTCGAGCT CGGTACCTTT 1141 AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG 1201 ACTGGAAGGG CTAATTCACT CCCAACGAAG ACAAGATCTG CTTTTTGCTT GTACTGGGTC 1261 TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT 1321 TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA 1381 CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGTAG 1441 TAGTTCATGT CATCTTATTA TTCAGTATTT ATAACTTGCA AAGAAATGAA TATCAGAGAG 1501 TGAGAGGAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA 1561 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA 1621 TGTATCTTAT CATGTCTGGC TCTAGCTATC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 1681 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 1741 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 1801 GGCCTAGGGA CGTACCCAAT TCGCCCTATA GTGAGTCGTA TTACGCGCGC TCACTGGCCG 1861 TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG 1921 CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC 1981 AACAGTTGCG CAGCCTGAAT GGCGAATGGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 2041 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 2101 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 2161 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 2221 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 2281 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 2341 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 2401 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA 2461 CAATTTAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA 2521 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA 2581 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC 2641 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA 2701 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT 2761 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG 2821 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA 2881 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT 2941 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT 3001 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA 3061 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA 3121 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA 3181 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC 3241 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC 3301 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG 3361 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT 3421 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA 3481 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT 3541 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA 3601 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 3661 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 3721 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT 3781 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 3841 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 3901 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 3961 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 4021 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 4081 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 4141 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 4201 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 4261 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC 4321 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT 4381 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT 4441 TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC 4501 AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC 4561 TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA 4621 TGATTACGCC AAGCGCGCAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG AGCTGCAAGC 4681 TTAATGTAGT CTTATGCAAT ACTCTTGTAG TCTTGCAACA TGGTAACGAT GAGTTAGCAA 4741 CATGCCTTAC AAGGAGAGAA AAAGCACCGT GCATGCCGAT TGGTGGAAGT AAGGTGGTAC 4801 GATCGTGCCT TATTAGGAAG GCAACAGACG GGTCTGACAT GGATTGGACG AACCACTGAA 4861 TTGCCGCATT GCAGAGATAT TGTATTTAAG TGCCTAGCTC GATACATAAA CGGGTCTCTC 4921 TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG 4981 CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT 5041 GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTGGCGCC 5101 CGAACAGGGA CTTGAAAGCG AAAGGGAAAC CAGAGGAGCT CTCTCGACGC AGGACTCGGC 5161 TTGCTGAAGC GCGCACGGCA AGAGGCGAGG GGCGGCGACT GGTGAGTACG CCAAAAATTT 5221 TGACTAGCGG AGGCTAGAAG GAGAGAGATG GGTGCGAGAG CGTCAGTATT AAGCGGGGGA 5281 GAATTAGATC GCGATGGGAA AAAATTCGGT TAAGGCCAGG GGGAAAGAAA AAATATAAAT 5341 TAAAACATAT AGTATGGGCA AGCAGGGAGC TAGAACGATT CGCAGTTAAT CCTGGCCTGT 5401 TAGAAACATC AGAAGGCTGT AGACAAATAC TGGGACAGCT ACAACCATCC CTTCAGACAG 5461 GATCAGAAGA ACTTAGATCA TTATATAATA CAGTAGCAAC CCTCTATTGT GTGCATCAAA 5521 GGATAGAGAT AAAAGACACC AAGGAAGCTT TAGACAAGAT AGAGGAAGAG CAAAACAAAA 5581 GTAAGACCAC CGCACAGCAA GCGGCCGCTG ATCTTCAGAC CTGGAGGAGG AGATATGAGG 5641 GACAATTGGA GAAGTGAATT ATATAAATAT AAAGTAGTAA AAATTGAACC ATTAGGAGTA 5701 GCACCCACCA AGGCAAAGAG AAGAGTGGTG CAGAGAGAAA AAAGAGCAGT GGGAATAGGA 5761 GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA GGAAGCACTA TGGGCGCAGC GTCAATGACG 5821 CTGACGGTAC AGGCCAGACA ATTATTGTCT GGTATAGTGC AGCAGCAGAA CAATTTGCTG 5881 AGGGCTATTG AGGCGCAACA GCATCTGTTG CAACTCACAG TCTGGGGCAT CAAGCAGCTC 5941 CAGGCAAGAA TCCTGGCTGT GGAAAGATAC CTAAAGGATC AACAGCTCCT GGGGATTTGG 6001 GGTTGCTCTG GAAAACTCAT TTGCACCACT GCTGTGCCTT GGAATGCTAG TTGGAGTAAT 6061 AAATCTCTGG AACAGATTTG GAATCACACG ACCTGGATGG AGTGGGACAG AGAAATTAAC 6121 AATTACACAA GCTTAATACA CTCCTTAATT GAAGAATCGC AAAACCAGCA AGAAAAGAAT 6181 GAACAAGAAT TATTGGAATT AGATAAATGG GCAAGTTTGT GGAATTGGTT TAACATAACA 6241 AATTGGCTGT GGTATATAAA ATTATTCATA ATGATAGTAG GAGGCTTGGT AGGTTTAAGA 6301 ATAGTTTTTG CTGTACTTTC TATAGTGAAT AGAGTTAGGC AGGGATATTC ACCATTATCG 6361 TTTCAGACCC ACCTCCCAAC CCCGAGGGGA CCCGACAGGC CCGAAGGAAT AGAAGAAGAA 6421 GGTGGAGAGA GAGACAGAGA CAGATCCATT CGATTAGTGA ACGGATCTCG ACGGTATCGA 6481 TAAGCTTGGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC 6541 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG 6601 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT 6661 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC 6721 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA 6781 TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG 6841 CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT 6901 TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA 6961 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT 7021 CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGACA CCGACTCTAG 7081 AGGATCCACT AGTCCAGTGT GGTGGAATTC TGCAGATATC AACAAGTTTG TACAAAAAAG 7141 CAGGCTCTTT AAAGGAACCA ATTCAGTCGA CTGGATCCGG TACCGAATTC ACCATGGAGG 7201 GGTCTAAGGC GTCCAGCAGC ACCATGCAGG TGAGCTTCGT GTGCCAGCGC TGTAGCCAGC 7261 CTCTGAAACT GGACACGAGC TTCAAGATCC TGGACCGGGT CACCATCCAG GAACTCACAG 7321 CTCCATTACT TACCACAGCC CAGGCGAAAC CAGGAGAGAC CCAGGAGGAA GAGGCTAACT 7381 CAGGAGAGGA GCCATTTATT GAAACTCGCC AGGATGGTGT CTCTCGAAGA TTCATCCCCC 7441 CAGCCAGGAT GATGTCTACA GAAAGTGCTA ATAGCTTCAC TCTGATCGGG GAGGCATCTG 7501 ATGGTGGCAC CATGGAGAAC CTCAGCCGGA GACTCAAGGT CACTGGAGAC CTGTTTGACA 7561 TCATGTCGGG CCAGACCGAT GTGGATTGAC ACTCGAGATA TCTAGACCCA GCTTT //