LOCUS pLenti6-mBeclin1-N-Frag-131-Flag-puro 7612 bp DNA circular SYN 09-FEB-2012 DEFINITION LMBP 8596. SOURCE ORGANISM COMMENT Accession number: LMBP 8596 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) DBSOURCE GenBank Unknown Unclassified FEATURES Location/Qualifiers Misc._recombina join(7606..7612,1..14) /label=attB1 Source join(<461..>483,<1..>56) /invitrogen="563" /label=Source_1 Vector join(461..>483,<1..56) /origin="pENTR3C (2.723bp circular) Target vector: from XhoI cut at 938^939 to EcoRI cut at 496^497, 2.281bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" Restriction_sit join(50..55,54^55) /note="Name: KpnI" /note="Pattern: ggtacc" /note="Number_of_matches: 2" /note="cut_0_on_positive_strand: 494^495" /note="cut_0_on_negative_strand: 490^491" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI CDS 65..457 /origin="mBeclin + stop + primers (1.384bp linear) Fragment: from EcoRI cut at 11^12 to XhoI cut at 1369^1370, 1.358bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label=mBeclin1-N-term_131_nostop Misc._recombina complement(477..497) /label=attB2 Misc._feature 541..565 /label="FLAG tag" CDS 578..586 /invitrogen="123000001" /note="Oriinal location: 8422..8430" /label="3 stops" Promoter 623..932 /invitrogen="2290000" /note="Oriinal location: 8467..8776" /label="SV40 early promoter" CDS 992..1594 /invitrogen="1050000" /note="Oriinal location: 1..603" /label=Puro(R) LTR 1685..1738 /invitrogen="1410000" /note="Oriinal location: 694..747" /label=deltaU3 LTR 1686..1919 /invitrogen="1390000" /note="Oriinal location: 695..928" /label="deltaU3/3' LTR" LTR 1739..1919 /invitrogen="1430000" /note="Oriinal location: 748..928" /label="HIV-1 3' LTR" PolyA_signal 1991..2121 /invitrogen="1840000" /note="Oriinal location: 1000..1130" /label="SV40 pA" Replication_ori 2494..2949 /invitrogen="2520000" /note="Oriinal location: 1503..1958" /label="f1 origin" Promoter 2981..3079 /invitrogen="2330000" /note="Oriinal location: 1990..2088" /label="bla promoter" CDS 3080..3940 /invitrogen="1020000" /note="Oriinal location: 2089..2949" /label=Amp(R) Replication_ori 4085..4758 /invitrogen="2540000" /note="Oriinal location: 3094..3767" /label="pUC origin" Promoter 5166..5394 /invitrogen="2270000" /note="Oriinal location: 4175..4403" /label="RSV promoter" Promoter 5166..5575 /invitrogen="2280000" /note="Oriinal location: 4175..4584" /label="RSV/5' LTR hybrid promoter" Source 5165^5166 /invitrogen="765" /note="Oriinal location: 4175..4174" /label=Source_1 LTR 5395..5575 /invitrogen="1430000" /note="Oriinal location: 4404..4584" /label="HIV-1 5' LTR" Misc._feature 5685 /invitrogen="123000012" /note="Oriinal location: 4694..4694" /label="splice donor" Misc._binding 5686..5730 /invitrogen="1780000" /note="Oriinal location: 4695..4739" /label="HIV-1 psi packaging signal" Protein_binding 6240..6473 /invitrogen="2440000" /note="Oriinal location: 5249..5482" /label="HIV-1 Rev response element" Misc._feature 6821 /invitrogen="123000013" /note="Oriinal location: 5830..5830" /label="splice acceptor" Misc._feature 6849 /invitrogen="123000014" /note="Oriinal location: 5858..5858" /label="splice acceptor" Promoter 6974..7556 /invitrogen="2170000" /note="Oriinal location: 5983..6565" /label="CMV promoter" TATA_box 7474..7477 /invitrogen="123000002" /note="Oriinal location: 6483..6486" /label="TATA box" ORIGIN 1 GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG ACTGGATCCG GTACCGAATT 61 CACCATGGAG GGGTCTAAGG CGTCCAGCAG CACCATGCAG GTGAGCTTCG TGTGCCAGCG 121 CTGTAGCCAG CCTCTGAAAC TGGACACGAG CTTCAAGATC CTGGACCGGG TCACCATCCA 181 GGAACTCACA GCTCCATTAC TTACCACAGC CCAGGCGAAA CCAGGAGAGA CCCAGGAGGA 241 AGAGGCTAAC TCAGGAGAGG AGCCATTTAT TGAAACTCGC CAGGATGGTG TCTCTCGAAG 301 ATTCATCCCC CCAGCCAGGA TGATGTCTAC AGAAAGTGCT AATAGCTTCA CTCTGATCGG 361 GGAGGCATCT GATGGTGGCA CCATGGAGAA CCTCAGCCGG AGACTCAAGG TCACTGGAGA 421 CCTGTTTGAC ATCATGTCGG GCCAGACCGA TGTGGATCAC TCGAGATATC TAGACCCAGC 481 TTTCTTGTAC AAAGTGGTTG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC 541 GGACTACAAG GATGACGATG ACAAAACGCG TACCGGTTAG TAATGAGTTT GGAATTAATT 601 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGG CAGGCAGAAG 661 TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC 721 AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT 781 AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG 841 ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA 901 GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT 961 ATCCATTTTC GGATCTGATC AGCACGTGAC CATGGCCACC GAGTACAAGC CCACGGTGCG 1021 CCTCGCCACC CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA 1081 CTACCCCGCC ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT 1141 GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA 1201 CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC 1261 CGAGATCGGC TCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT 1321 GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG 1381 CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA 1441 GGCGGCCGAG CGCGCTGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC 1501 CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG 1561 CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGAGCTAAG CACAATTCGA GCTCGGTACC 1621 TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG 1681 GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT CTGCTTTTTG CTTGTACTGG 1741 GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT 1801 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG 1861 TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG 1921 TAGTAGTTCA TGTCATCTTA TTATTCAGTA TTTATAACTT GCAAAGAAAT GAATATCAGA 1981 GAGTGAGAGG AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC 2041 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT 2101 CAATGTATCT TATCATGTCT GGCTCTAGCT ATCCCGCCCC TAACTCCGCC CATCCCGCCC 2161 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 2221 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 2281 GGAGGCCTAG GGACGTACCC AATTCGCCCT ATAGTGAGTC GTATTACGCG CGCTCACTGG 2341 CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG 2401 CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT 2461 CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGGACGCGCC CTGTAGCGGC GCATTAAGCG 2521 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 2581 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 2641 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 2701 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 2761 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 2821 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 2881 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC 2941 TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT 3001 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA 3061 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT 3121 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC 3181 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT 3241 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT 3301 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA 3361 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG 3421 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA 3481 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG 3541 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA 3601 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG 3661 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT 3721 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG 3781 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC 3841 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA 3901 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC 3961 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT 4021 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC 4081 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 4141 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 4201 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT 4261 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 4321 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 4381 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 4441 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 4501 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 4561 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 4621 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 4681 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 4741 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT 4801 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC 4861 AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC 4921 GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA 4981 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC 5041 GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA 5101 CCATGATTAC GCCAAGCGCG CAATTAACCC TCACTAAAGG GAACAAAAGC TGGAGCTGCA 5161 AGCTTAATGT AGTCTTATGC AATACTCTTG TAGTCTTGCA ACATGGTAAC GATGAGTTAG 5221 CAACATGCCT TACAAGGAGA GAAAAAGCAC CGTGCATGCC GATTGGTGGA AGTAAGGTGG 5281 TACGATCGTG CCTTATTAGG AAGGCAACAG ACGGGTCTGA CATGGATTGG ACGAACCACT 5341 GAATTGCCGC ATTGCAGAGA TATTGTATTT AAGTGCCTAG CTCGATACAT AAACGGGTCT 5401 CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT 5461 AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC 5521 TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC 5581 GCCCGAACAG GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC 5641 GGCTTGCTGA AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA 5701 TTTTGACTAG CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG 5761 GGAGAATTAG ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA 5821 AATTAAAACA TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC 5881 TGTTAGAAAC ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA 5941 CAGGATCAGA AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC 6001 AAAGGATAGA GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA 6061 AAAGTAAGAC CACCGCACAG CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG 6121 AGGGACAATT GGAGAAGTGA ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA 6181 GTAGCACCCA CCAAGGCAAA GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA 6241 GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG 6301 ACGCTGACGG TACAGGCCAG ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG 6361 CTGAGGGCTA TTGAGGCGCA ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG 6421 CTCCAGGCAA GAATCCTGGC TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT 6481 TGGGGTTGCT CTGGAAAACT CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT 6541 AATAAATCTC TGGAACAGAT TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT 6601 AACAATTACA CAAGCTTAAT ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG 6661 AATGAACAAG AATTATTGGA ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA 6721 ACAAATTGGC TGTGGTATAT AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA 6781 AGAATAGTTT TTGCTGTACT TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA 6841 TCGTTTCAGA CCCACCTCCC AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA 6901 GAAGGTGGAG AGAGAGACAG AGACAGATCC ATTCGATTAG TGAACGGATC TCGACGGTAT 6961 CGATAAGCTT GGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG 7021 CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA 7081 GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA 7141 CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC 7201 GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC 7261 GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA 7321 TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG 7381 TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG 7441 CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC 7501 CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGACTC 7561 TAGAGGATCC ACTAGTCCAG TGTGGTGGAA TTCTGCAGAT ATCAACAAGT TT //