LOCUS       pLenti6-mBeclin1-N-Frag-147-puro      7663 bp    DNA     circular SYN 09-FEB-2012
DEFINITION  LMBP 8597.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8597
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
DBSOURCE    GenBank
            Unknown
            Unclassified
FEATURES             Location/Qualifiers
     Source          join(<1..>56,<512..>534)
                     /invitrogen="563"
                     /label=Source_1
     Misc._recombina join(7657..7663,1..14)
                     /label=attB1
     Vector          join(<1..56,512..>534)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
     CDS             65..508
                     /origin="mBeclin + stop + primers (1.384bp linear)
                     Fragment: from EcoRI cut at 11^12 to XhoI cut at
                     1369^1370, 1.358bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /label="Beclin1_N-term_147 + stop"
     Misc._recombina complement(528..548)
                     /label=attB2
     Misc._feature   592..616
                     /label="FLAG tag"
     CDS             629..637
                     /invitrogen="123000001"
                     /note="Oriinal location: 8422..8430"
                     /label="3 stops"
     Promoter        674..983
                     /invitrogen="2290000"
                     /note="Oriinal location: 8467..8776"
                     /label="SV40 early promoter"
     CDS             1043..1645
                     /invitrogen="1050000"
                     /note="Oriinal location: 1..603"
                     /label=Puro(R)
     LTR             1736..1789
                     /invitrogen="1410000"
                     /note="Oriinal location: 694..747"
                     /label=deltaU3
     LTR             1737..1970
                     /invitrogen="1390000"
                     /note="Oriinal location: 695..928"
                     /label="deltaU3/3' LTR"
     LTR             1790..1970
                     /invitrogen="1430000"
                     /note="Oriinal location: 748..928"
                     /label="HIV-1 3' LTR"
     PolyA_signal    2042..2172
                     /invitrogen="1840000"
                     /note="Oriinal location: 1000..1130"
                     /label="SV40 pA"
     Replication_ori 2545..3000
                     /invitrogen="2520000"
                     /note="Oriinal location: 1503..1958"
                     /label="f1 origin"
     Promoter        3032..3130
                     /invitrogen="2330000"
                     /note="Oriinal location: 1990..2088"
                     /label="bla promoter"
     CDS             3131..3991
                     /invitrogen="1020000"
                     /note="Oriinal location: 2089..2949"
                     /label=Amp(R)
     Replication_ori 4136..4809
                     /invitrogen="2540000"
                     /note="Oriinal location: 3094..3767"
                     /label="pUC origin"
     Source          5216^5217
                     /invitrogen="765"
                     /note="Oriinal location: 4175..4174"
                     /label=Source_1
     Promoter        5217..5445
                     /invitrogen="2270000"
                     /note="Oriinal location: 4175..4403"
                     /label="RSV promoter"
     Promoter        5217..5626
                     /invitrogen="2280000"
                     /note="Oriinal location: 4175..4584"
                     /label="RSV/5' LTR hybrid promoter"
     LTR             5446..5626
                     /invitrogen="1430000"
                     /note="Oriinal location: 4404..4584"
                     /label="HIV-1 5' LTR"
     Misc._feature   5736
                     /invitrogen="123000012"
                     /note="Oriinal location: 4694..4694"
                     /label="splice donor"
     Misc._binding   5737..5781
                     /invitrogen="1780000"
                     /note="Oriinal location: 4695..4739"
                     /label="HIV-1 psi packaging signal"
     Protein_binding 6291..6524
                     /invitrogen="2440000"
                     /note="Oriinal location: 5249..5482"
                     /label="HIV-1 Rev response element"
     Misc._feature   6872
                     /invitrogen="123000013"
                     /note="Oriinal location: 5830..5830"
                     /label="splice acceptor"
     Misc._feature   6900
                     /invitrogen="123000014"
                     /note="Oriinal location: 5858..5858"
                     /label="splice acceptor"
     Promoter        7025..7607
                     /invitrogen="2170000"
                     /note="Oriinal location: 5983..6565"
                     /label="CMV promoter"
     TATA_box        7525..7528
                     /invitrogen="123000002"
                     /note="Oriinal location: 6483..6486"
                     /label="TATA box"
ORIGIN
        1 GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG ACTGGATCCG GTACCGAATT
       61 CACCATGGAG GGGTCTAAGG CGTCCAGCAG CACCATGCAG GTGAGCTTCG TGTGCCAGCG
      121 CTGTAGCCAG CCTCTGAAAC TGGACACGAG CTTCAAGATC CTGGACCGGG TCACCATCCA
      181 GGAACTCACA GCTCCATTAC TTACCACAGC CCAGGCGAAA CCAGGAGAGA CCCAGGAGGA
      241 AGAGGCTAAC TCAGGAGAGG AGCCATTTAT TGAAACTCGC CAGGATGGTG TCTCTCGAAG
      301 ATTCATCCCC CCAGCCAGGA TGATGTCTAC AGAAAGTGCT AATAGCTTCA CTCTGATCGG
      361 GGAGGCATCT GATGGTGGCA CCATGGAGAA CCTCAGCCGG AGACTCAAGG TCACTGGAGA
      421 CCTGTTTGAC ATCATGTCGG GCCAGACCGA TGTGGATCAC CCGCTGTGTG AGGAATGCAC
      481 AGACACTCTT TTAGACCAGC TGGACTGACA CTCGAGATAT CTAGACCCAG CTTTCTTGTA
      541 CAAAGTGGTT GATATCCAGC ACAGTGGCGG CCGCTCGAGT CTAGAGGGCC CGGACTACAA
      601 GGATGACGAT GACAAAACGC GTACCGGTTA GTAATGAGTT TGGAATTAAT TCTGTGGAAT
      661 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG GCAGGCAGAA GTATGCAAAG
      721 CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG
      781 AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC
      841 CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT
      901 TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG
      961 AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT
     1021 CGGATCTGAT CAGCACGTGA CCATGGCCAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC
     1081 CCGCGACGAC GTCCCCCGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC
     1141 CACGCGCCAC ACCGTCGACC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT
     1201 CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC
     1261 GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG
     1321 CTCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT
     1381 CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC
     1441 CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA
     1501 GCGCGCTGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA
     1561 GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG
     1621 CATGACCCGC AAGCCCGGTG CCTGAGCTAA GCACAATTCG AGCTCGGTAC CTTTAAGACC
     1681 AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA
     1741 AGGGCTAATT CACTCCCAAC GAAGACAAGA TCTGCTTTTT GCTTGTACTG GGTCTCTCTG
     1801 GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC
     1861 TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG
     1921 TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTAGTAGTTC
     1981 ATGTCATCTT ATTATTCAGT ATTTATAACT TGCAAAGAAA TGAATATCAG AGAGTGAGAG
     2041 GAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC
     2101 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC
     2161 TTATCATGTC TGGCTCTAGC TATCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG
     2221 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC
     2281 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA
     2341 GGGACGTACC CAATTCGCCC TATAGTGAGT CGTATTACGC GCGCTCACTG GCCGTCGTTT
     2401 TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC
     2461 CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT
     2521 TGCGCAGCCT GAATGGCGAA TGGGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG
     2581 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG
     2641 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG
     2701 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT
     2761 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT
     2821 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA
     2881 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA
     2941 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT
     3001 AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA
     3061 TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA
     3121 AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT
     3181 TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA
     3241 GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG
     3301 TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC
     3361 GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA
     3421 GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT
     3481 AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT
     3541 GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT
     3601 AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA
     3661 CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT
     3721 TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC
     3781 ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA
     3841 GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT
     3901 AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA
     3961 GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT
     4021 TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA
     4081 TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT
     4141 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA
     4201 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT
     4261 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA
     4321 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT
     4381 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC
     4441 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA
     4501 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA
     4561 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG
     4621 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT
     4681 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG
     4741 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT
     4801 TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT
     4861 TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA
     4921 GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA
     4981 ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA
     5041 TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT
     5101 GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA
     5161 CGCCAAGCGC GCAATTAACC CTCACTAAAG GGAACAAAAG CTGGAGCTGC AAGCTTAATG
     5221 TAGTCTTATG CAATACTCTT GTAGTCTTGC AACATGGTAA CGATGAGTTA GCAACATGCC
     5281 TTACAAGGAG AGAAAAAGCA CCGTGCATGC CGATTGGTGG AAGTAAGGTG GTACGATCGT
     5341 GCCTTATTAG GAAGGCAACA GACGGGTCTG ACATGGATTG GACGAACCAC TGAATTGCCG
     5401 CATTGCAGAG ATATTGTATT TAAGTGCCTA GCTCGATACA TAAACGGGTC TCTCTGGTTA
     5461 GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA
     5521 TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC
     5581 TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA
     5641 GGGACTTGAA AGCGAAAGGG AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG
     5701 AAGCGCGCAC GGCAAGAGGC GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA
     5761 GCGGAGGCTA GAAGGAGAGA GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA
     5821 GATCGCGATG GGAAAAAATT CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC
     5881 ATATAGTATG GGCAAGCAGG GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA
     5941 CATCAGAAGG CTGTAGACAA ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG
     6001 AAGAACTTAG ATCATTATAT AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG
     6061 AGATAAAAGA CACCAAGGAA GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA
     6121 CCACCGCACA GCAAGCGGCC GCTGATCTTC AGACCTGGAG GAGGAGATAT GAGGGACAAT
     6181 TGGAGAAGTG AATTATATAA ATATAAAGTA GTAAAAATTG AACCATTAGG AGTAGCACCC
     6241 ACCAAGGCAA AGAGAAGAGT GGTGCAGAGA GAAAAAAGAG CAGTGGGAAT AGGAGCTTTG
     6301 TTCCTTGGGT TCTTGGGAGC AGCAGGAAGC ACTATGGGCG CAGCGTCAAT GACGCTGACG
     6361 GTACAGGCCA GACAATTATT GTCTGGTATA GTGCAGCAGC AGAACAATTT GCTGAGGGCT
     6421 ATTGAGGCGC AACAGCATCT GTTGCAACTC ACAGTCTGGG GCATCAAGCA GCTCCAGGCA
     6481 AGAATCCTGG CTGTGGAAAG ATACCTAAAG GATCAACAGC TCCTGGGGAT TTGGGGTTGC
     6541 TCTGGAAAAC TCATTTGCAC CACTGCTGTG CCTTGGAATG CTAGTTGGAG TAATAAATCT
     6601 CTGGAACAGA TTTGGAATCA CACGACCTGG ATGGAGTGGG ACAGAGAAAT TAACAATTAC
     6661 ACAAGCTTAA TACACTCCTT AATTGAAGAA TCGCAAAACC AGCAAGAAAA GAATGAACAA
     6721 GAATTATTGG AATTAGATAA ATGGGCAAGT TTGTGGAATT GGTTTAACAT AACAAATTGG
     6781 CTGTGGTATA TAAAATTATT CATAATGATA GTAGGAGGCT TGGTAGGTTT AAGAATAGTT
     6841 TTTGCTGTAC TTTCTATAGT GAATAGAGTT AGGCAGGGAT ATTCACCATT ATCGTTTCAG
     6901 ACCCACCTCC CAACCCCGAG GGGACCCGAC AGGCCCGAAG GAATAGAAGA AGAAGGTGGA
     6961 GAGAGAGACA GAGACAGATC CATTCGATTA GTGAACGGAT CTCGACGGTA TCGATAAGCT
     7021 TGGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC
     7081 CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC
     7141 CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG
     7201 TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT
     7261 TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC
     7321 ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT
     7381 GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC
     7441 CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC
     7501 GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC GTTTAGTGAA CCGTCAGATC
     7561 GCCTGGAGAC GCCATCCACG CTGTTTTGAC CTCCATAGAA GACACCGACT CTAGAGGATC
     7621 CACTAGTCCA GTGTGGTGGA ATTCTGCAGA TATCAACAAG TTT
//
