LOCUS pDG2i-mBeclin1-N-Frag-122-Flag-puro 8620 bp DNA circular SYN 09-FEB-2012 DEFINITION LMBP 8599. SOURCE ORGANISM COMMENT Accession number: LMBP 8599 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) DBSOURCE GenBank Unknown Unclassified FEATURES Location/Qualifiers Source join(<434..>456,<1..>56) /invitrogen="563" /label=Source_1 Misc._recombina join(8614..8620,1..14) /label=attB1 Vector join(434..>456,<1..56) /origin="pENTR3C (2.723bp circular) Target vector: from XhoI cut at 938^939 to EcoRI cut at 496^497, 2.281bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" CDS 65..430 /origin="mBeclin + stop + primers (1.384bp linear) Fragment: from EcoRI cut at 11^12 to XhoI cut at 1369^1370, 1.358bp Orientation: original Plus strand 5': no change Plus strand 3': no change Minus strand 5': no change Minus strand 3': no change" /label="Beclin1_N-term_122 - stop" Misc._recombina complement(450..470) /label=attB2 Misc._feature 515..538 /note="Oriinal location: 44..67" /label="FLAG tag" CDS 551..559 /invitrogen="123000001" /note="Oriinal location: 80..88" /label="3 stops" Promoter 596..905 /invitrogen="2290000" /note="Oriinal location: 125..434" /label="SV40 early promoter" CDS 965..1662 /note="Oriinal location: <494..1191" /label=rtTA3 Misc._feature 1728..2303 /note="Oriinal location: <1257..1832" /label=ires CDS 2304..2906 /note="Oriinal location: <1833..2435" /label=PuroR LTR 2997..3050 /invitrogen="1410000" /note="Oriinal location: 2526..2579" /label=deltaU3 LTR 2998..3231 /invitrogen="1390000" /note="Oriinal location: 2527..2760" /label="deltaU3/3' LTR" LTR 3051..3231 /invitrogen="1430000" /note="Oriinal location: 2580..2760" /label="HIV-1 3' LTR" PolyA_signal 3303..3433 /invitrogen="1840000" /note="Oriinal location: 2832..2962" /label="SV40 pA" Replication_ori 3806..4261 /invitrogen="2520000" /note="Oriinal location: 3335..3790" /label="f1 origin" Promoter 4293..4391 /invitrogen="2330000" /note="Oriinal location: 3822..3920" /label="bla promoter" CDS 4392..5252 /invitrogen="1020000" /note="Oriinal location: 3921..4781" /label=Amp(R) Replication_ori 5397..6070 /invitrogen="2540000" /note="Oriinal location: 4926..5599" /label="pUC origin" Promoter 6478..6706 /invitrogen="2270000" /note="Oriinal location: 6007..6235" /label="RSV promoter" Promoter 6478..6887 /invitrogen="2280000" /note="Oriinal location: 6007..6416" /label="RSV/5' LTR hybrid promoter" LTR 6707..6887 /invitrogen="1430000" /note="Oriinal location: 6236..6416" /label="HIV-1 5' LTR" Misc._feature 6997 /invitrogen="123000012" /note="Oriinal location: 6526..6526" /label="splice donor" Misc._binding 6998..7042 /invitrogen="1780000" /note="Oriinal location: 6527..6571" /label="HIV-1 psi packaging signal" Protein_binding 7552..7785 /invitrogen="2440000" /note="Oriinal location: 7081..7314" /label="HIV-1 Rev response element" Misc._feature 8133 /invitrogen="123000013" /note="Oriinal location: 7662..7662" /label="splice acceptor" Misc._feature 8161 /invitrogen="123000014" /note="Oriinal location: 7690..7690" /label="splice acceptor" Misc._feature 8303..8498 /note="Oriinal location: 7832..8027" /label=TRE Misc._feature 8513..8570 /note="Oriinal location: <8042..8099" /label=CMVprom ORIGIN 1 GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG ACTGGATCCG GTACCGAATT 61 CACCATGGAG GGGTCTAAGG CGTCCAGCAG CACCATGCAG GTGAGCTTCG TGTGCCAGCG 121 CTGTAGCCAG CCTCTGAAAC TGGACACGAG CTTCAAGATC CTGGACCGGG TCACCATCCA 181 GGAACTCACA GCTCCATTAC TTACCACAGC CCAGGCGAAA CCAGGAGAGA CCCAGGAGGA 241 AGAGGCTAAC TCAGGAGAGG AGCCATTTAT TGAAACTCGC CAGGATGGTG TCTCTCGAAG 301 ATTCATCCCC CCAGCCAGGA TGATGTCTAC AGAAAGTGCT AATAGCTTCA CTCTGATCGG 361 GGAGGCATCT GATGGTGGCA CCATGGAGAA CCTCAGCCGG AGACTCAAGG TCACTGGAGA 421 CCTGTTTGAC CACTCGAGAT ATCTAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCCA 481 GCACAGTGGC GGCCGCTCGA GTCTAGAGGG CCCGGACTAC AAGGATGACG ATGACAAAAC 541 GCGTACCGGT TAGTAATGAG TTTGGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT 601 GGAAAGTCCC CAGGCTCCCC AGGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC 661 AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA 721 TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC 781 GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC 841 CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT 901 AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT 961 GACCATGTCT AGGCTGGACA AGAGCAAAGT CATAAACGGA GCTCTGGAAT TACTCAATGG 1021 TGTCGGTATC GAAGGCCTGA CGACAAGGAA ACTCGCTCAA AAGCTGGGAG TTGAGCAGCC 1081 TACCCTGTAC TGGCACGTGA AGAACAAGCG GGCCCTGCTC GATGCCCTGC CAATCGAGAT 1141 GCTGGACAGG CATCATACCC ACTTCTGCCC CCTGGAAGGC GAGTCATGGC AAGACTTTCT 1201 GCGGAACAAC GCCAAGTCAT ACCGCTGTGC TCTCCTCTCA CATCGCGACG GGGCTAAAGT 1261 GCATCTCGGC ACCCGCCCAA CAGAGAAACA GTACGAAACC CTGGAAAATC AGCTCGCGTT 1321 CCTGTGTCAG CAAGGCTTCT CCCTGGAGAA CGCACTGTAC GCTCTGTCCG CCGTGGGCCA 1381 CTTTACACTG GGCTGCGTAT TGGAGGAACA GGAGCATCAA GTAGCAAAAG AGGAAAGAGA 1441 GACACCTACC ACCGATTCTA TGCCCCCACT TCTGAGACAA GCAATTGAGC TGTTCGACCG 1501 GCAGGGAGCC GAACCTGCCT TCCTTTTCGG CCTGGAACTA ATCATATGTG GCCTGGAGAA 1561 ACAGCTAAAG TGCGAAAGCG GCGGGCCGAC CGACGCCCTT GACGATTTTG ACTTAGACAT 1621 GCTCCCAGCC GATGCCCTTG ACGATTTTGA CCTTGACATG CTCCCCGGGT AAATAACTTC 1681 GTATAGTATA CATTATACGA AGTTATGGAT CCGCGGCCGC AAATTCCGCC CCTCTCCCTC 1741 CCCCCCCCCT AACGTTACTG GCCGAAGCCG CTTGGAATAA GGCCGGTGTG CGTTTGTCTA 1801 TATGTTATTT TCCACCATAT TGCCGTCTTT TGGCAATGTG AGGGCCCGGA AACCTGGCCC 1861 TGTCTTCTTG ACGAGCATTC CTAGGGGTCT TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT 1921 GTTGAATGTC GTGAAGGAAG CAGTTCCTCT GGAAGCTTCT TGAAGACAAA CAACGTCTGT 1981 AGCGACCCTT TGCAGGCAGC GGAACCCCCC ACCTGGCGAC AGGTGCCTCT GCGGCCAAAA 2041 GCCACGTGTA TAAGATACAC CTGCAAAGGC GGCACAACCC CAGTGCCACG TTGTGAGTTG 2101 GATAGTTGTG GAAAGAGTCA AATGGCTCTC CTCAAGCGTA TTCAACAAGG GGCTGAAGGA 2161 TGCCCAGAAG GTACCCCATT GTATGGGATC TGATCTGGGG CCTCGGTGCA CATGCTTTAC 2221 ATGTGTTTAG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG ACGTGGTTTT 2281 CCTTTGAAAA ACACGATAAT ACCATGGCCA CCGAGTACAA GCCCACGGTG CGCCTCGCCA 2341 CCCGCGACGA CGTCCCCCGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG 2401 CCACGCGCCA CACCGTCGAC CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC 2461 TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG 2521 CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG 2581 GCTCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC 2641 TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC 2701 CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG 2761 AGCGCGCTGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG 2821 AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT 2881 GCATGACCCG CAAGCCCGGT GCCTGAGCTA AGCACAATTC GAGCTCGGTA CCTTTAAGAC 2941 CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT AAAAGAAAAG GGGGGACTGG 3001 AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATCTGCTTTT TGCTTGTACT GGGTCTCTCT 3061 GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC 3121 CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG 3181 GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTAGTAGTT 3241 CATGTCATCT TATTATTCAG TATTTATAAC TTGCAAAGAA ATGAATATCA GAGAGTGAGA 3301 GGAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 3361 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 3421 CTTATCATGT CTGGCTCTAG CTATCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC 3481 GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC 3541 CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT 3601 AGGGACGTAC CCAATTCGCC CTATAGTGAG TCGTATTACG CGCGCTCACT GGCCGTCGTT 3661 TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT 3721 CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG 3781 TTGCGCAGCC TGAATGGCGA ATGGGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 3841 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 3901 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 3961 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 4021 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 4081 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 4141 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 4201 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT 4261 TAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC 4321 ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA 4381 AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT 4441 TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC 4501 AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA 4561 GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG 4621 CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC 4681 AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG 4741 TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC 4801 TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG 4861 TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG 4921 ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC 4981 TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC 5041 CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG 5101 AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG 5161 TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG 5221 AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC 5281 TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG 5341 ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG 5401 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC 5461 AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC 5521 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT 5581 AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC 5641 TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT 5701 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC 5761 AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG 5821 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG 5881 GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG 5941 TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA 6001 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT 6061 TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT 6121 TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG 6181 AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT 6241 AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA 6301 ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA 6361 TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT 6421 ACGCCAAGCG CGCAATTAAC CCTCACTAAA GGGAACAAAA GCTGGAGCTG CAAGCTTAAT 6481 GTAGTCTTAT GCAATACTCT TGTAGTCTTG CAACATGGTA ACGATGAGTT AGCAACATGC 6541 CTTACAAGGA GAGAAAAAGC ACCGTGCATG CCGATTGGTG GAAGTAAGGT GGTACGATCG 6601 TGCCTTATTA GGAAGGCAAC AGACGGGTCT GACATGGATT GGACGAACCA CTGAATTGCC 6661 GCATTGCAGA GATATTGTAT TTAAGTGCCT AGCTCGATAC ATAAACGGGT CTCTCTGGTT 6721 AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA 6781 ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA 6841 CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC 6901 AGGGACTTGA AAGCGAAAGG GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT 6961 GAAGCGCGCA CGGCAAGAGG CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT 7021 AGCGGAGGCT AGAAGGAGAG AGATGGGTGC GAGAGCGTCA GTATTAAGCG GGGGAGAATT 7081 AGATCGCGAT GGGAAAAAAT TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA 7141 CATATAGTAT GGGCAAGCAG GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA 7201 ACATCAGAAG GCTGTAGACA AATACTGGGA CAGCTACAAC CATCCCTTCA GACAGGATCA 7261 GAAGAACTTA GATCATTATA TAATACAGTA GCAACCCTCT ATTGTGTGCA TCAAAGGATA 7321 GAGATAAAAG ACACCAAGGA AGCTTTAGAC AAGATAGAGG AAGAGCAAAA CAAAAGTAAG 7381 ACCACCGCAC AGCAAGCGGC CGCTGATCTT CAGACCTGGA GGAGGAGATA TGAGGGACAA 7441 TTGGAGAAGT GAATTATATA AATATAAAGT AGTAAAAATT GAACCATTAG GAGTAGCACC 7501 CACCAAGGCA AAGAGAAGAG TGGTGCAGAG AGAAAAAAGA GCAGTGGGAA TAGGAGCTTT 7561 GTTCCTTGGG TTCTTGGGAG CAGCAGGAAG CACTATGGGC GCAGCGTCAA TGACGCTGAC 7621 GGTACAGGCC AGACAATTAT TGTCTGGTAT AGTGCAGCAG CAGAACAATT TGCTGAGGGC 7681 TATTGAGGCG CAACAGCATC TGTTGCAACT CACAGTCTGG GGCATCAAGC AGCTCCAGGC 7741 AAGAATCCTG GCTGTGGAAA GATACCTAAA GGATCAACAG CTCCTGGGGA TTTGGGGTTG 7801 CTCTGGAAAA CTCATTTGCA CCACTGCTGT GCCTTGGAAT GCTAGTTGGA GTAATAAATC 7861 TCTGGAACAG ATTTGGAATC ACACGACCTG GATGGAGTGG GACAGAGAAA TTAACAATTA 7921 CACAAGCTTA ATACACTCCT TAATTGAAGA ATCGCAAAAC CAGCAAGAAA AGAATGAACA 7981 AGAATTATTG GAATTAGATA AATGGGCAAG TTTGTGGAAT TGGTTTAACA TAACAAATTG 8041 GCTGTGGTAT ATAAAATTAT TCATAATGAT AGTAGGAGGC TTGGTAGGTT TAAGAATAGT 8101 TTTTGCTGTA CTTTCTATAG TGAATAGAGT TAGGCAGGGA TATTCACCAT TATCGTTTCA 8161 GACCCACCTC CCAACCCCGA GGGGACCCGA CAGGCCCGAA GGAATAGAAG AAGAAGGTGG 8221 AGAGAGAGAC AGAGACAGAT CCATTCGATT AGTGAACGGA TCTCGACGGT ATCGATGGTC 8281 CGAGGTTCTA GACGAGTTTA CTCCCTATCA GTGATAGAGA ACGATGTCGA GTTTACTCCC 8341 TATCAGTGAT AGAGAACGTA TGTCGAGTTT ACTCCCTATC AGTGATAGAG AACGTATGTC 8401 GAGTTTACTC CCTATCAGTG ATAGAGAACG TATGTCGAGT TTATCCCTAT CAGTGATAGA 8461 GAACGTATGT CGAGTTTACT CCCTATCAGT GATAGAGAAC GTATGTCGAG GTAGGCGTGT 8521 ACGGTGGGAG GCCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CACCGGTCAC 8581 TAGTCCAGTG TGGTGGAATT CTGCAGATAT CAACAAGTTT //