LOCUS       pENTR3C-mBeclin1-N-Frag-147-stop        2733 bp    DNA     circular SYN 19-MAR-1980
DEFINITION  LMBP 8605.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8605
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Unknown Unclassified.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENTR3C-mBeclin1-N-Frag-147-stop|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 2733 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 1 1910 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 3 445 0 2286 0 2579 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 2714 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2726 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 318 0 2733 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 571 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "")) 
COMMENT       (CObList 
COMMENT        #10=(CFSignal (CObList) "Beclin1_N-term_147 -  stop" 4 0 0 1 441 0 
COMMENT             (CStringList) 
COMMENT             (CStringList "/origin=\"mBeclin + stop + primers (1.384bp linear)" 
COMMENT              "Fragment: from EcoRI cut at 11^12 to XhoI cut at" 
COMMENT              "1369^1370, 1.358bp           Orientation: original" 
COMMENT              "Plus strand 5':   no change           Plus strand 3':   no" 
COMMENT              "change           Minus strand 5':  no change" 
COMMENT              "Minus strand 3':  no change\"") 1 1 1 1 "1..441") 
COMMENT        #11=(CFSignal (CObList) "attL2" 21 0 1 458 557 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2680000\"") 1 1 1 1 
COMMENT             "complement(458..557)") 
COMMENT        #12=(CFSignal (CObList) "Kan(R)" 4 0 0 680 1489 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1210000\"") 1 1 1 1 "680..1489") 
COMMENT        #13=(CFSignal (CObList) "pUC origin" 21 0 0 1553 2226 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2540000\"") 1 1 1 1 "1553..2226") 
COMMENT        #14=(CFSignal (CObList) "rrnB T1 transcription terminator" 21 0 1 2335 
COMMENT             2378 0 (CStringList) (CStringList "/invitrogen=\"2580000\"") 1 1 1 
COMMENT             1 "complement(2335..2378)") 
COMMENT        #15=(CFSignal (CObList) "rrnB T2 transcription terminator" 21 0 1 2510 
COMMENT             2537 0 (CStringList) (CStringList "/invitrogen=\"2590000\"") 1 1 1 
COMMENT             1 "complement(2510..2537)") 
COMMENT        #16=(CFSignal (CObList) "attL1" 21 0 0 2587 2686 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2670000\"") 1 1 1 1 "2587..2686")) 
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COMMENT                          "DNA 'pENTR3C-mBeclin1-N-Frag-147-stop'" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 2733 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Division: SYN" 1) 
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COMMENT                          "Modification Date in the Original DB: 09-FEB-2012" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/pENTR3C\">pENTR3C</a>" 
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COMMENT                          "Sequence Source: Unknown (unknown)" 1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/Unknown\">Unknown(Unknown)</a>" 
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COMMENT                          "Taxonomy: Unknown Unclassified" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "CYCGRG" "GRGCYC") 
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FEATURES             Location/Qualifiers
     CDS             1..441
                     /origin="mBeclin + stop + primers (1.384bp linear)
                     Fragment: from EcoRI cut at 11^12 to XhoI cut at
                     1369^1370, 1.358bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /vntifkey="4"
                     /label=Beclin1_N-term_147\-\\stop
     Misc._recombina complement(458..557)
                     /invitrogen="2680000"
                     /vntifkey="21"
                     /label=attL2
                     /note="Unknown feature type:Misc._recombina"
     CDS             680..1489
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     Replication_ori 1553..2226
                     /invitrogen="2540000"
                     /vntifkey="21"
                     /label=pUC\origin
                     /note="Unknown feature type:Replication_ori"
     Terminator      complement(2335..2378)
                     /invitrogen="2580000"
                     /vntifkey="21"
                     /label=rrnB\T1\transcription\terminator
                     /note="Unknown feature type:Terminator"
     Terminator      complement(2510..2537)
                     /invitrogen="2590000"
                     /vntifkey="21"
                     /label=rrnB\T2\transcription\terminator
                     /note="Unknown feature type:Terminator"
     Misc._recombina 2587..2686
                     /invitrogen="2670000"
                     /vntifkey="21"
                     /label=attL1
                     /note="Unknown feature type:Misc._recombina"
BASE COUNT      709 a       634 c       680 g       710 t 
ORIGIN
        1 atggaggggt ctaaggcgtc cagcagcacc atgcaggtga gcttcgtgtg ccagcgctgt 
       61 agccagcctc tgaaactgga cacgagcttc aagatcctgg accgggtcac catccaggaa 
      121 ctcacagctc cattacttac cacagcccag gcgaaaccag gagagaccca ggaggaagag 
      181 gctaactcag gagaggagcc atttattgaa actcgccagg atggtgtctc tcgaagattc 
      241 atccccccag ccaggatgat gtctacagaa agtgctaata gcttcactct gatcggggag 
      301 gcatctgatg gtggcaccat ggagaacctc agccggagac tcaaggtcac tggagacctg 
      361 tttgacatca tgtcgggcca gaccgatgtg gatcacccgc tgtgtgagga atgcacagac 
      421 actcttttag accagctgga ccactcgaga tatctagacc cagctttctt gtacaaagtt 
      481 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa 
      541 aataaaatca ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg 
      601 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa 
      661 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa 
      721 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc 
      781 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca 
      841 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac 
      901 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt 
      961 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc 
     1021 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt 
     1081 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat 
     1141 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga 
     1201 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca 
     1261 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga 
     1321 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct 
     1381 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc 
     1441 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt 
     1501 taattggttg taacattatt cagattgggc cccgttccac tgagcgtcag accccgtaga 
     1561 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 
     1621 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 
     1681 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 
     1741 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 
     1801 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 
     1861 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 
     1921 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 
     1981 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 
     2041 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 
     2101 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 
     2161 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 
     2221 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgctagcat 
     2281 ggatctcggg gacgtctaac tactaagcga gagtagggaa ctgccaggca tcaaataaaa 
     2341 cgaaaggctc agtcggaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 
     2401 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga 
     2461 gggtggcggg caggacgccc gccataaact gccaggcatc aaactaagca gaaggccatc 
     2521 ctgacggatg gcctttttgc gtttctacaa actcttcctg ttagttagtt acttaagctc 
     2581 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat 
     2641 aagcaatgct tttttataat gccaactttg tacaaaaaag caggctcttt aaaggaacca 
     2701 attcagtcga ctggatccgg taccgaattc acc 
//
