LOCUS       pDONOR207-PRS-mRIP1-      5368 bp    DNA     circular UNA 10-FEB-2012
DEFINITION  LMBP 8609.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8609
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
DBSOURCE    GenBank
FEATURES             Location/Qualifiers
     Misc._feature   complement(join(5361..5368,1..88))
                     /note="Oriinal location: complement(5361..88)"
                     /label=attL2
     Primer          complement(113..136)
                     /invitrogen="1994000"
                     /note="Oriinal location: complement(113..136)"
                     /label="pDONR?E07 reverse primer"
     CDS             complement(872..1405)
                     /invitrogen="1150000"
                     /note="Oriinal location: complement(872..1405)"
                     /label=Gm(R)
     Promoter        complement(1594..1621)
                     /invitrogen="2250000"
                     /note="Oriinal location: complement(1594..1621)"
                     /label="Pc promoter"
     Terminator      3002..3029
                     /invitrogen="2590000"
                     /note="Oriinal location: 3002..3029"
                     /label="rrnB T2 transcription terminator"
     Terminator      3161..3204
                     /invitrogen="2580000"
                     /note="Oriinal location: 3161..3204"
                     /label="rrnB T1 transcription terminator"
     Primer          3229..3253
                     /invitrogen="1993000"
                     /note="Oriinal location: 3229..3253"
                     /label="pDONR?E07 forward primer"
     Misc._feature   3261..3356
                     /note="Oriinal location: 3261..3356"
                     /label=attL1
     Cleavage_site   3362..3385
                     /label="PRS (prescission cleavage site)"
     CDS             3386..5356
                     /label="mRIP1 (FL)+ stop"
ORIGIN
        1 TTGTACAAAG TGGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC
       61 ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG CAGCTCTGGC CCGTGTCTCA
      121 AAATCTCTGA TGTTACATTG CACAAGATAA AAATATATCA TCATGAACAA TAAAACTGTC
      181 TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG
      241 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA
      301 TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT
      361 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT
      421 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA
      481 TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA
      541 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA
      601 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC
      661 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG
      721 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC
      781 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAGG
      841 CCGGGAAGCC GATCTCGGCT TGAACGAATT GTTAGGTGGC GGTACTTGGG TCGATATCAA
      901 AGTGCATCAC TTCTTCCCGT ATGCCCAACT TTGTATAGAG AGCCACTGCG GGATCGTCAC
      961 CGTAATCTGC TTGCACGTAG ATCACATAAG CACCAAGCGC GTTGGCCTCA TGCTTGAGGA
     1021 GATTGATGAG CGCGGTGGCA ATGCCCTGCC TCCGGTGCTC GCCGGAGACT GCGAGATCAT
     1081 AGATATAGAT CTCACTACGC GGCTGCTCAA ACCTGGGCAG AACGTAAGCC GCGAGAGCGC
     1141 CAACAACCGC TTCTTGGTCG AAGGCAGCAA GCGCGATGAA TGTCTTACTA CGGAGCAAGT
     1201 TCCCGAGGTA ATCGGAGTCC GGCTGATGTT GGGAGTAGGT GGCTACGTCT CCGAACTCAC
     1261 GACCGAAAAG ATCAAGAGCA GCCCGCATGG ATTTGACTTG GTCAGGGCCG AGCCTACATG
     1321 TGCGAATGAT GCCCATACTT GAGCCACCTA ACTTTGTTTT AGGGCGACTG CCCTGCTGCG
     1381 TAACATCGTT GCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCAAACA TCGACCCACG
     1441 GCGTAACGCG CTTGCTGCTT GGATGCCCGA GGCATAGACT GTACAAAAAA ACAGTCATAA
     1501 CAAGCCATGA AAACCGCCAC TGCGCCGTTA CCACCGCTGC GTTCGGTCAA GGTTCTGGAC
     1561 CAGTTGCGTG AGCGCATACG CTACTTGCAT TACAGTTTAC GAACCGAACA GGCTTATGTC
     1621 AACTGGGTTC GTGCCTTCAT CCGTTTCCAC GGTGTGCGTC ACCCGGCAAC CTTGGGCAGC
     1681 AGCGAAGTCG AGGCATTTCT GTCCTGGCTG GCGAACGAGC GCAAGGTTTC GGTCTCCACG
     1741 CATCGTCAGG CATTGGCGGC CTTGCTGTTC TTCTACGGCA AGGTGCTGTG CACGGATCTG
     1801 CCCTGGCTTC AGGAGATCGG AAGACCTCGG CCGTCGCGGC GCTTGCCGGT GGTGCTGACC
     1861 CCGGATGAAG TGGTTCGCAT CCTCGGTTTT CTGGAAGGCG AGCATCGTTT GTTCGCCCAG
     1921 GACTCTAGCT ATAGTTCTAG TGGTTGGCTA CTAATAGGTT GTATTGATGT TGGACGAGTC
     1981 GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT
     2041 CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA
     2101 TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA
     2161 CACTGGCAGA GCATTACGCT GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT
     2221 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT
     2281 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG
     2341 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA
     2401 GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA
     2461 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG
     2521 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG
     2581 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT
     2641 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA
     2701 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC
     2761 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG
     2821 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG
     2881 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT
     2941 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCTAGCCA GGAAGAGTTT GTAGAAACGC
     3001 AAAAAGGCCA TCCGTCAGGA TGGCCTTCTG CTTAGTTTGA TGCCTGGCAG TTTATGGCGG
     3061 GCGTCCTGCC CGCCACCCTC CGGGCCGTTG CTTCACAACG TTCAAATCCG CTCCCGGCGG
     3121 ATTTGTCCTA CTCAGGAGAG CGTTCACCGA CAAACAACAG ATAAAACGAA AGGCCCAGTC
     3181 TTCCGACTGA GCCTTTCGTT TTATTTGATG CCTGGCAGTT CCCTACTCTC GCGTTAACGC
     3241 TAGCATGGAT CTCGGGCCCC AAATAATGAT TTTATTTTGA CTGATAGTGA CCTGTTCGTT
     3301 GCAACAAATT GATGAGCAAT GCTTTTTTAT AATGCCAAGT TTGTACAAAA AAGCAGGCTT
     3361 ACTGGAAGTT CTGTTCCAGG GGCCCATGCA ACCAGACATG TCCTTGGACA ATATTAAGAT
     3421 GGCATCCAGT GACCTGCTGG AGAAGACAGA CCTAGACAGC GGAGGCTTCG GGAAGGTGTC
     3481 CTTGTGTTAC CACAGAAGCC ATGGATTTGT CATCCTGAAA AAAGTATACA CAGGGCCCAA
     3541 CCGCGCTGAG TACAATGAGG TTCTCTTGGA AGAGGGGAAG ATGATGCACA GACTGAGACA
     3601 CAGTCGAGTG GTGAAGCTAC TGGGCATCAT CATAGAAGAA GGGAACTATT CGCTGGTGAT
     3661 GGAGTACATG GAGAAGGGCA ACCTGATGCA CGTGCTAAAG ACCCAGATAG ATGTCCCACT
     3721 TTCATTGAAA GGAAGGATAA TCGTGGAGGC CATAGAAGGC ATGTGCTACT TACATGACAA
     3781 AGGTGTGATA CACAAGGACC TGAAGCCTGA GAATATCCTC GTTGATCGTG ACTTTCACAT
     3841 TAAGATAGCC GATCTTGGTG TGGCTTCCTT TAAGACATGG AGCAAACTGA CTAAGGAGAA
     3901 AGACAACAAG CAGAAAGAAG TGAGCAGCAC CACTAAGAAG AACAATGGTG GTACCCTTTA
     3961 CTACATGGCA CCCGAACACC TGAATGACAT CAATGCAAAG CCCACGGAGA AGTCGGACGT
     4021 GTACAGCTTT GGCATTGTCC TTTGGGCAAT ATTTGCAAAA AAGGAGCCCT ATGAGAATGT
     4081 CATCTGTACT GAGCAGTTCG TGATCTGCAT AAAATCTGGG AACAGGCCAA ATGTAGAGGA
     4141 AATCCTTGAG TACTGTCCAA GGGAGATCAT CAGCCTCATG GAGCGGTGCT GGCAGGCGAT
     4201 CCCAGAAGAC AGGCCAACAT TTCTTGGCAT TGAAGAAGAA TTTAGGCCTT TTTACTTAAG
     4261 TCATTTTGAA GAATATGTAG AAGAGGATGT GGCAAGTTTA AAGAAAGAGT ATCCAGATCA
     4321 AAGCCCAGTG CTGCAGAGAA TGTTTTCACT GCAGCATGAC TGTGTACCCT TACCTCCGAG
     4381 CAGGTCAAAT TCAGAACAAC CTGGATCGCT GCACAGTTCC CAGGGGCTCC AGATGGGTCC
     4441 TGTGGAGGAG TCCTGGTTTT CTTCCTCCCC AGAGTACCCA CAGGACGAGA ATGATCGCAG
     4501 TGTGCAGGCT AAGCTGCAAG AGGAAGCCAG CTATCATGCT TTTGGAATAT TTGCAGAGAA
     4561 ACAGACAAAA CCGCAGCCAA GGCAGAATGA GGCTTACAAC AGAGAGGAGG AAAGGAAACG
     4621 AAGGGTCTCT CATGACCCCT TTGCACAGCA GAGAGCTCGT GAGAATATTA AGAGTGCAGG
     4681 AGCAAGAGGT CATTCTGATC CCAGCACAAC GAGTCGTGGA ATTGCAGTGC AACAGCTGTC
     4741 ATGGCCAGCC ACCCAAACAG TTTGGAACAA TGGATTGTAT AATCAGCATG GATTTGGAAC
     4801 TACAGGTACA GGAGTTTGGT ATCCGCCAAA TCTAAGCCAA ATGTATAGTA CTTATAAAAC
     4861 TCCAGTGCCA GAGACCAACA TACCGGGAAG CACACCCACC ATGCCATACT TCTCTGGGCC
     4921 AGTAGCAGAT GACCTCATAA AATATACTAT ATTCAATAGT TCTGGTATTC AGATTGGAAA
     4981 CCACAATTAT ATGGATGTTG GACTGAATTC ACAACCACCA AACAATACTT GCAAAGAAGA
     5041 GTCGACTTCC AGACACCAAG CCATCTTTGA TAACACCACT AGTCTGACTG ATGAACACCT
     5101 GAACCCTATC AGGGAAAACC TGGGAAGGCA GTGGAAAAAC TGTGCCCGCA AGCTGGGCTT
     5161 CACTGAGTCT CAGATCGATG AAATCGACCA TGACTATGAA AGAGATGGAC TGAAAGAGAA
     5221 AGTTTACCAA ATGCTTCAGA AGTGGCTGAT GCGGGAAGGC ACCAAAGGGG CCACAGTGGG
     5281 AAAGTTGGCC CAGGCACTTC ACCAATGTTG CAGGATAGAC CTGCTGAACC ACTTGATTCG
     5341 TGCCAGCCAG AGCTAATACC CAGCTTTC
//
