LOCUS       pENTR3C-hSFRS2        2955 bp    DNA     circular     13-FEB-2012
DEFINITION  LMBP 8616.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8616
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|617729556|
COMMENT     VNTDBDATE|617729556|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENTR3C-hSFRS2|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pENTR3C-hSFRS2" 0 2955 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 1 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI" "XhoI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "oIB0037 - hSFRS2 FW" 
COMMENT             "ATCCGGTACCGAATTCACCATGAGCTACGGCCGCCCCC" "forward primer hSFRS2" 0 
COMMENT             (CStringList) 0)) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 1 0 0 0 0 0 
COMMENT        "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 1 0 0 0 0 1 2955 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #1=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 1 2132 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 3 667 0 2508 0 2801 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 2936 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2948 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 222 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 793 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 667 0 0 "")) 
COMMENT       (CObList 
COMMENT        #12=(CFSignal (CObList) "rrnB T2 transcription terminator" 43 0 1 2732 
COMMENT             2759 0 (CStringList) (CStringList "/invitrogen=\"2590000\"") 1 1 1 
COMMENT             1 "complement(2731..2758)") 
COMMENT        #13=(CFSignal (CObList) "rrnB T1 transcription terminator" 43 0 1 2557 
COMMENT             2600 0 (CStringList) (CStringList "/invitrogen=\"2580000\"") 1 1 1 
COMMENT             1 "complement(2556..2599)") 
COMMENT        #14=(CFSignal (CObList) "pUC origin" 33 0 0 1775 2448 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2540000\"") 1 1 1 1 "1774..2447") 
COMMENT        #15=(CFSignal (CObList) "Kan(R)" 4 0 0 902 1711 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1210000\"") 1 1 1 1 "901..1710") 
COMMENT        #16=(CFSignal (CObList) "attL2" 86 0 1 680 779 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2680000\"") 1 1 1 1 
COMMENT             "complement(679..778)") 
COMMENT        #17=(CFSignal (CObList) "attL1" 86 0 0 2809 2908 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2670000\"") 1 1 1 1 "2808..2907") 
COMMENT        #18=(CFSignal (CObList) "hSFRS2" 4 0 0 1 663 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1..663")) 
COMMENT       (CObList 
COMMENT        #19=(CMSite "oIB0037 - hSFRS2 FW" 
COMMENT             "ATCCGGTACCGAATTCACCATGAGCTACGGCCGCCCCC" "forward primer hSFRS2" 1 
COMMENT             2937 0 100 100 100)) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CTextView 0 
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COMMENT                    "pENTR3C-hSFRS2" 2) 5 "" 0 4) 
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COMMENT                    (CObjectList 
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COMMENT                          "DNA 'pENTR3C-hSFRS2'" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 02/13/12 03:32" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 02/13/12 03:32" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 2955 bp" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 11-JUN-2010" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                   "Annotations") 
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COMMENT                                        " N1: 2132 " 1))))) "ApaLI: 1 site") 1 5 
COMMENT                          "GTGCAC" "CACGTG") 
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COMMENT                                        " N1: 667 " 1) 
COMMENT                                   #41=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 2508 " 1) 
COMMENT                                   #42=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #2# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 2801 " 1))))) "AvaI: 3 sites") 1 5 
COMMENT                          "CYCGRG" "GRGCYC") 
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COMMENT                          (CFolderPar 
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COMMENT                                        (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 2936 " 1))))) "BamHI: 1 site") 1 5 
COMMENT                          "GGATCC" "CCTAGG") 
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COMMENT                          (CFolderPar 
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COMMENT                            (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT" 
COMMENT                          "TAGCTA") 
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COMMENT                                        (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 2948 " 1))))) "EcoRI: 1 site") 1 5 
COMMENT                          "GAATTC" "CTTAAG") 
COMMENT                     #50=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 84224776 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" 
COMMENT                          "TTCGAA") 
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COMMENT                                  (CObjectList 
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COMMENT                                        " N1: 222 " 1))))) "NcoI: 1 site") 1 5 
COMMENT                          "CCATGG" "GGTACC") 
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COMMENT                                        " N1: 793 " 1))))) "PstI: 1 site") 5 1 
COMMENT                          "CTGCAG" "GACGTC") 
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COMMENT                          "GGGCCC") 
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COMMENT                                        " N1: 667 " 1))))) "XhoI: 1 site") 1 5 
COMMENT                          "CTCGAG" "GAGCTC") 
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COMMENT                          "GGGCCC"))) "Restriction/Methylation Map") 
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COMMENT                          "   Similarity between ambiguous nucleotides (if any)" 
COMMENT                          1) 
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COMMENT                          "is calculated as an AVERAGE similarity" 1) 
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COMMENT                                          #68=(COligo "oIB0037 - hSFRS2 FW" 
COMMENT                                               "ATCCGGTACCGAATTCACCATGAGCTACGGCCGCCCCC" 
COMMENT                                               "forward primer hSFRS2" 0 
COMMENT                                               (CStringList) 0) 0) 1 2 2 0 181) 
COMMENT                                        " ATCCGGTACCGAATTCACCATGAGCTACGGCCGCCCCC" 
COMMENT                                        1)) 
COMMENT                                  #69=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                       "forward primer hSFRS2" 1) 
COMMENT                                  #70=(CLinePar 
COMMENT                                       (CParagraph 0 (2 #19# 1) 1 2 2 0 193) 
COMMENT                                       " N1: position 2937 similarity 100.0% " 1))))) 
COMMENT                          "oIB0037 - hSFRS2 FW (1 site)"))) 
COMMENT                   "Motifs (both strands, best fit only)") 
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COMMENT                    "<a href=\"ivgnlink:/iq\">Invitrogen products related to pENTR3C-hSFRS2</a>" 
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COMMENT                                        "Start: 1     End: 663 " 1) 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   901..1710" 1))) "Kan(R)"))) 
COMMENT                          "CDS (2 total)") 
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COMMENT                                   #86=(CLinePar 
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COMMENT                                   #87=(CLinePar 
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COMMENT                                        "   1774..2447" 1))) "pUC origin"))) 
COMMENT                          "Replication Origin (1 total)") 
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COMMENT                                        "Start: 2557  End: 2600 (Complementary)" 
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COMMENT                                 "rrnB T1 transcription terminator") 
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COMMENT                                 "rrnB T2 transcription terminator"))) 
COMMENT                          "Terminator (2 total)") 
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COMMENT                                          "   2808..2907" 1))) "attL1"))) 
COMMENT                          "Misc. Recombination (2 total)"))) "Feature Map")))) 
COMMENT       (CGraphView 
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FEATURES             Location/Qualifiers
     terminator      complement(2732..2759)
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      complement(2557..2600)
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     rep_origin      1775..2448
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     CDS             902..1711
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     misc_recomb     complement(680..779)
                     /invitrogen="2680000"
                     /vntifkey="86"
                     /label=attL2
     misc_recomb     2809..2908
                     /invitrogen="2670000"
                     /vntifkey="86"
                     /label=attL1
     CDS             1..663
                     /vntifkey="4"
                     /label=hSFRS2
BASE COUNT      709 a       753 c       761 g       732 t 
ORIGIN
        1 atgagctacg gccgcccccc tcccgatgtg gagggtatga cctctctcaa ggtggacaac 
       61 ctgacctacc gcacctcgcc cgacacgctg aggcgcgtct tcgagaagta cgggcgcgtc 
      121 ggcgacgtgt acatcccgcg ggaccgctac accaaggagt cccgcggctt cgccttcgtt 
      181 cgctttcacg acaagcgcga cgctgaggac gctatggatg ccatggacgg ggccgtgctg 
      241 gacggccgcg agctgcgggt gcaaatggcg cgctacggcc gccccccgga ctcacaccac 
      301 agccgccggg gaccgccacc ccgcaggtac gggggcggtg gctacggacg ccggagccgc 
      361 agccctaggc ggcgtcgccg cagccgatcc cggagtcgga gccgttccag gtctcgcagc 
      421 cgatctcgct acagccgctc gaagtctcgg tcccgcactc gttctcgatc tcggtcgacc 
      481 tccaagtcca gatccgcacg aaggtccaag tccaagtcct cgtcggtctc cagatctcgt 
      541 tcgcggtcca ggtcccggtc tcggtccagg agtcctcccc cagtgtccaa gagggaatcc 
      601 aaatccaggt cgcgatcgaa gagtcccccc aagtctcctg aagaggaagg agcggtgtcc 
      661 tctcactcga gatatctaga cccagctttc ttgtacaaag ttggcattat aagaaagcat 
      721 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc 
      781 catccagctg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa 
      841 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt 
      901 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga 
      961 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg 
     1021 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc 
     1081 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc 
     1141 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc 
     1201 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga 
     1261 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa 
     1321 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga 
     1381 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat 
     1441 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga 
     1501 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat 
     1561 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc 
     1621 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca 
     1681 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacatta 
     1741 ttcagattgg gccccgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 
     1801 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 
     1861 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 
     1921 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 
     1981 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 
     2041 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 
     2101 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 
     2161 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 
     2221 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 
     2281 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 
     2341 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 
     2401 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 
     2461 gttatcccct gattctgtgg ataaccgtat taccgctagc atggatctcg gggacgtcta 
     2521 actactaagc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcggaa 
     2581 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat 
     2641 ccgccgggag cggatttgaa cgttgtgaag caacggcccg gagggtggcg ggcaggacgc 
     2701 ccgccataaa ctgccaggca tcaaactaag cagaaggcca tcctgacgga tggccttttt 
     2761 gcgtttctac aaactcttcc tgttagttag ttacttaagc tcgggcccca aataatgatt 
     2821 ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg cttttttata 
     2881 atgccaactt tgtacaaaaa agcaggctct ttaaaggaac caattcagtc gactggatcc 
     2941 ggtaccgaat tcacc 
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