LOCUS       pENTR3C-mPRKAR1A        3435 bp    DNA     circular     11-JUN-2010
DEFINITION  LMBP 8624.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8624
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|556709955|
COMMENT     VNTDBDATE|556710068|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENTR3C-mPRKAR1A|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 128 0 229 0 1002 0 1023 0 
COMMENT            1147 0 2988 0 3281 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 3416 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
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COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 138 0 3435 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 912 0 1273 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 1025 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 1023 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 229 0 1147 0 0 "")) 
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COMMENT             "complement(1159..1258)") 
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COMMENT             (CStringList "/invitrogen=\"2670000\"") 1 1 1 1 "3288..3387") 
COMMENT        #17=(CFSignal (CObList) "mPRKAR1AV1" 4 0 0 1 1143 0 (CStringList) 
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COMMENT                          "Last Modified: 06/11/10 09:41" 1) 
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FEATURES             Location/Qualifiers
     terminator      complement(3212..3239)
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      complement(3037..3080)
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     rep_origin      2255..2928
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     CDS             1382..2191
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     misc_recomb     complement(1160..1259)
                     /invitrogen="2680000"
                     /vntifkey="86"
                     /label=attL2
     misc_recomb     3289..3388
                     /invitrogen="2670000"
                     /vntifkey="86"
                     /label=attL1
     CDS             1..1143
                     /vntifkey="4"
                     /label=mPRKAR1AV1
BASE COUNT      888 a       761 c       893 g       893 t 
ORIGIN
        1 atggcgtctg gcagtatggc aaccagtgag gaagagcgga gtctccggga atgcgagctc 
       61 tatgtgcaga agcacaatat ccaggccctg ctgaaggact ccatcgtgca gctgtgcact 
      121 acgcggcccg agaggcccat ggcattcctt cgggaatact ttgagaggtt ggagaaggag 
      181 gaggcaagac agattcagtg tctacagaaa accggcatcc gtactgactc gagggaggac 
      241 gagatctctc ctccaccccc caatccagtg gtgaagggcc gacggcgccg aggtgctatc 
      301 agtgctgaag tttacactga ggaggatgct gcctcctacg ttagaaaggt tattccaaaa 
      361 gattataaga caatggctgc tttagccaag gccatcgaaa agaatgtgct gttttcacac 
      421 cttgatgata acgagagaag tgacattttt gatgctatgt ttccagtctc ctttattgct 
      481 ggagagacgg ttattcagca aggtgatgaa ggggataact tctatgtgat tgatcaagga 
      541 gaaatggatg tctatgtcaa taatgaatgg gcaaccagtg ttggggaagg agggagcttt 
      601 ggagagctgg ctttgattta tggaacaccc agagcagcca ctgtcaaagc aaagacaaac 
      661 gtgaaactgt ggggcatcga ccgagacagc taccgaagaa tcctcatggg aagcactctg 
      721 cgaaagagga agatgtatga agaattcctt agtaaagtgt ctattttaga gtctctggac 
      781 aagtgggagc gtctcacagt agccgatgca ttggagcctg tccagtttga agatggacag 
      841 aagatcgtgg tgcaaggaga gccgggggat gagttcttca tcattttaga gggcacagct 
      901 gctgtgctgc agcgtcggtc agaaaacgaa gaatttgttg aagtgggacg actggggcct 
      961 tctgattatt ttggtgaaat tgccctgctg atgaatcgtc ctcgggctgc cactgtggtt 
     1021 gcccggggcc ctttgaagtg cgttaagttg gaccggcctc ggtttgaacg cgtccttggc 
     1081 ccgtgctcag acatcctcaa gcggaacatc cagcagtaca acagcttcgt gtccctgtcc 
     1141 gtccactcga gatatctaga cccagctttc ttgtacaaag ttggcattat aagaaagcat 
     1201 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc 
     1261 catccagctg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa 
     1321 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt 
     1381 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga 
     1441 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg 
     1501 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc 
     1561 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc 
     1621 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc 
     1681 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga 
     1741 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa 
     1801 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga 
     1861 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat 
     1921 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga 
     1981 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat 
     2041 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc 
     2101 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca 
     2161 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacatta 
     2221 ttcagattgg gccccgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 
     2281 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 
     2341 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 
     2401 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 
     2461 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 
     2521 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 
     2581 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 
     2641 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 
     2701 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 
     2761 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 
     2821 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 
     2881 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 
     2941 gttatcccct gattctgtgg ataaccgtat taccgctagc atggatctcg gggacgtcta 
     3001 actactaagc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcggaa 
     3061 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat 
     3121 ccgccgggag cggatttgaa cgttgtgaag caacggcccg gagggtggcg ggcaggacgc 
     3181 ccgccataaa ctgccaggca tcaaactaag cagaaggcca tcctgacgga tggccttttt 
     3241 gcgtttctac aaactcttcc tgttagttag ttacttaagc tcgggcccca aataatgatt 
     3301 ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg cttttttata 
     3361 atgccaactt tgtacaaaaa agcaggctct ttaaaggaac caattcagtc gactggatcc 
     3421 ggtaccgaat tcacc 
//
