LOCUS       pENTR3C-mPINK1-E      4054 bp    DNA     circular SYN 10-FEB-2012
DEFINITION  LMBP 8628.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8628
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
DBSOURCE    GenBank
FEATURES             Location/Qualifiers
     Source          1..4054
                     /invitrogen="563"
                     /note="Oriinal location: 1..4054"
                     /label=Source_1
     Terminator      complement(106..149)
                     /invitrogen="2580000"
                     /note="Oriinal location: complement(106..149)"
                     /label="rrnB T1 transcription terminator"
     Terminator      complement(281..308)
                     /invitrogen="2590000"
                     /note="Oriinal location: complement(281..308)"
                     /label="rrnB T2 transcription terminator"
     Primer_binding_ 301..320
                     /note="Oriinal location: 301..320"
                     /label=FVR3592
     Misc._recombina 358..457
                     /invitrogen="2670000"
                     /note="Oriinal location: 358..457"
                     /label=attL1
     CDS             483..2222
                     /note="Oriinal location: 483..2273"
                     /label=mPink1
     Misc._feature   2223..2270
                     /note="Oriinal location: 2223..2270"
                     /label=E-tag
     Misc._recombina complement(2283..2382)
                     /invitrogen="2680000"
                     /note="Oriinal location: complement(2283..2382)"
                     /label=attL2
     Primer_binding_ 2500..2520
                     /note="Oriinal location: 2500..2520"
                     /label=FVR3257
     CDS             2505..3314
                     /invitrogen="1210000"
                     /note="Oriinal location: 2505..3314"
                     /label=Kan(R)
     Replication_ori 3378..4051
                     /invitrogen="2540000"
                     /note="Oriinal location: 3378..4051"
                     /label="pUC origin"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG
       61 GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT
      121 CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
      181 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG
      241 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT
      301 GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA
      361 ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC
      421 TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG
      481 ACATGGCGGT GCGACAGGCA CTGGGCCGAG GCCTGCAGCT GGGTCGGGCG CTGCTGCTGC
      541 GCTTCGCGCC CAAGCCCGGC CCGCTGTTCG GCTGGGGGAA GCCCGGCCCC GCGGCGGCCT
      601 GGGGCCGCGG AGAGCGCCCA GGCCAGGTCG TAAGCCCCGG AGCACAGCCT CGGCCGGTCG
      661 GGCTCCCCCT CCCGGACCGC TACCGCTTCT TCCGCCAGTC GGTAGCTGGG CTGGCGGCGC
      721 GGATCCAGAG GCAGTTCATG GTGCGGGCCC GGGGCGGCGC AGGCCCTTGC GGCCGAGCGG
      781 TCTTCCTGGC CTTCGGGCTG GGGCTGGGGC TGATCGAGGA GAAGCAGGCG GAAGGCCGGA
      841 GGGCCGCCTC GGCCTGTCAG GAGATCCAGG CAATTTTTAC ACAGAAAACC AAGCGCGTGT
      901 CTGACCCACT GGACACTCGA TGCTGGCAGG GCTTCCGTCT GGAGGATTAT CTGATAGGGC
      961 AAGCCATTGG CAAGGGTTGC AATGCCGCTG TGTATGAAGC CACCATGCCC ACGCTGCCCC
     1021 AGCACCTGGA AAAGGCCAAA CACCTTGGCC TTATAGGAAA GGGCCCGGAT GTCGTCCTGA
     1081 AGGGAGCAGA CGGGGAGCAG GCTCCAGGGA CTCCCACCTT TCCCTTTGCC ATCAAGATGA
     1141 TGTGGAATAT CTCGGCAGGT TCCTCCAGCG AAGCCATCTT AAGCAAAATG AGCCAGGAGC
     1201 TGGTCCCGGC AAGCCGCGTG GCTTTGGCTG GAGAGTATGG AGCAGTTACT TACAGAAGAT
     1261 CCAGAGATGG TCCCAAGCAG CTTGCCCCAC ACCCTAACAT CATCCGGGTT TTCCGCGCCT
     1321 TCACCTCATC TGTGCCCCTC CTGCCGGGGG CCCTGGCTGA CTATCCTGAT ATGCTGCCCC
     1381 CACACTACTA CCCAGAAGGC CTGGGCCACG GTCGCACACT GTTCCTCGTT ATGAAGAACT
     1441 ACCCCTGTAC CCTGCGCCAG TACCTTGAGG AGCAGACTCC CAGTTCTCGC CTGGCTACCA
     1501 TGATGACCTT GCAGTTGCTG GAGGGCGTGG ACCATCTGGT TCAGCAGGGC ATTGCCCATC
     1561 GGGATCTCAA GTCCGACAAC ATCCTTGTGG AGTGGGACTC AGATGGCTGT CCCTGGCTAG
     1621 TGATCTCAGA CTTTGGCTGC TGCCTGGCTG ACCAGCATGT TGGCCTGCGG CTGCCTTTCA
     1681 ACAGCTCCAG TGTAGAGCGT GGTGGCAATG GCTCCCTGAT GGCCCCTGAG GTGTCCACAG
     1741 CCCATTCTGG CCCCAGTGCG GTAATTGACT ACAGCAAAGC CGATACCTGG GCTGTGGGGG
     1801 CCATCGCCTA TGAAATCTTT GGGCTTGCCA ATCCCTTCTA TGGCCAAGGC AGTGCCCACC
     1861 TCGAGAGCCG CAGCTACCAG GAAGCTCAGC TGCCTGAGAT GCCTGAGTCG GTGCCTCCAG
     1921 AGGCAAGACG GCTGGTGAGG TCACTGCTCC AGCGAGAGGC CAGCAAGAGA CCGTCTGCAC
     1981 GCTTAGCTGC AAATGTGCTG CACTTAAGCC TCTGGGGCGA GCATCTTCTA GCCCTGAAGA
     2041 ACCTGAAATT GGACAAGATG ATCGCCTGGC TCTTGCAGCA GTCAGCAGCC ACTCTGCTGG
     2101 CTGACAGGCT GAGAGAGAAG AGCTGCGTGG AGACAAAGCT GCAGATGCTG TTTCTGGCTA
     2161 ACCTGGAGTG TGAGGCTCTC TGCCAGGCAG CCCTCCTCCT CTCTTCCTGG AGGGCAGCCC
     2221 CAGGTGCGCC GGTGCCGTAT CCGGACCCGC TGGAACCGCG TGCGGCCGCT TGAGATATCT
     2281 AGACCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG
     2341 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGCAGCTCT
     2401 GGCCCGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA TAAAAATATA TCATCATGAA
     2461 CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGAGC CATATTCAAC
     2521 GGGAAACGTC GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT GGGTATAAAT
     2581 GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA TCGCTTGTAT GGGAAGCCCG
     2641 ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT GTTACAGATG
     2701 AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC AAGCATTTTA
     2761 TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGAAAA ACAGCATTCC
     2821 AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG GCAGTGTTCC
     2881 TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT CGCGTATTTC
     2941 GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT GATTTTGATG
     3001 ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAA CTTTTGCCAT
     3061 TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT ATTTTTGACG
     3121 AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC CGATACCAGG
     3181 ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT
     3241 TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT TTGATGCTCG
     3301 ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA TTATTCAGAT TGGGCCCCGT
     3361 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC
     3421 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC
     3481 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC
     3541 CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC
     3601 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT
     3661 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT
     3721 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT
     3781 ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT
     3841 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG
     3901 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT
     3961 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT
     4021 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT
//
