LOCUS       pENTR207-PRS-hRIP1-M92A(KD)      4837 bp    DNA     circular UNA 10-FEB-2012
DEFINITION  LMBP 8636.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8636
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
FEATURES             Location/Qualifiers
     Misc._feature   complement(join(4830..4837,1..88))
                     /label=attL2
     Primer          complement(113..136)
                     /invitrogen="1994000"
                     /label="pDONR207 reverse primer"
     CDS             complement(872..1405)
                     /invitrogen="1150000"
                     /label=Gm(R)
     Promoter        complement(1594..1621)
                     /invitrogen="2250000"
                     /label="Pc promoter"
     Terminator      3002..3029
                     /invitrogen="2590000"
                     /label="rrnB T2 transcription terminator"
     Terminator      3161..3204
                     /invitrogen="2580000"
                     /label="rrnB T1 transcription terminator"
     Primer          3229..3253
                     /invitrogen="1993000"
                     /label="pDONR207 forward primer"
     Misc._feature   3261..3356
                     /label=attL1
     Cleavage_site   3362..3385
                     /label="PRS (prescission cleavage site)"
     CDS             3386..4825
                     /note="Region on donor sequence (yves pO14 fragment):
                     14..1507"
                     /label="hRIP1M92A(KD) + stop"
ORIGIN
        1 TTGTACAAAG TGGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC
       61 ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG CAGCTCTGGC CCGTGTCTCA
      121 AAATCTCTGA TGTTACATTG CACAAGATAA AAATATATCA TCATGAACAA TAAAACTGTC
      181 TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG
      241 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA
      301 TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT
      361 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT
      421 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA
      481 TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA
      541 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA
      601 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC
      661 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG
      721 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC
      781 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAGG
      841 CCGGGAAGCC GATCTCGGCT TGAACGAATT GTTAGGTGGC GGTACTTGGG TCGATATCAA
      901 AGTGCATCAC TTCTTCCCGT ATGCCCAACT TTGTATAGAG AGCCACTGCG GGATCGTCAC
      961 CGTAATCTGC TTGCACGTAG ATCACATAAG CACCAAGCGC GTTGGCCTCA TGCTTGAGGA
     1021 GATTGATGAG CGCGGTGGCA ATGCCCTGCC TCCGGTGCTC GCCGGAGACT GCGAGATCAT
     1081 AGATATAGAT CTCACTACGC GGCTGCTCAA ACCTGGGCAG AACGTAAGCC GCGAGAGCGC
     1141 CAACAACCGC TTCTTGGTCG AAGGCAGCAA GCGCGATGAA TGTCTTACTA CGGAGCAAGT
     1201 TCCCGAGGTA ATCGGAGTCC GGCTGATGTT GGGAGTAGGT GGCTACGTCT CCGAACTCAC
     1261 GACCGAAAAG ATCAAGAGCA GCCCGCATGG ATTTGACTTG GTCAGGGCCG AGCCTACATG
     1321 TGCGAATGAT GCCCATACTT GAGCCACCTA ACTTTGTTTT AGGGCGACTG CCCTGCTGCG
     1381 TAACATCGTT GCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCAAACA TCGACCCACG
     1441 GCGTAACGCG CTTGCTGCTT GGATGCCCGA GGCATAGACT GTACAAAAAA ACAGTCATAA
     1501 CAAGCCATGA AAACCGCCAC TGCGCCGTTA CCACCGCTGC GTTCGGTCAA GGTTCTGGAC
     1561 CAGTTGCGTG AGCGCATACG CTACTTGCAT TACAGTTTAC GAACCGAACA GGCTTATGTC
     1621 AACTGGGTTC GTGCCTTCAT CCGTTTCCAC GGTGTGCGTC ACCCGGCAAC CTTGGGCAGC
     1681 AGCGAAGTCG AGGCATTTCT GTCCTGGCTG GCGAACGAGC GCAAGGTTTC GGTCTCCACG
     1741 CATCGTCAGG CATTGGCGGC CTTGCTGTTC TTCTACGGCA AGGTGCTGTG CACGGATCTG
     1801 CCCTGGCTTC AGGAGATCGG AAGACCTCGG CCGTCGCGGC GCTTGCCGGT GGTGCTGACC
     1861 CCGGATGAAG TGGTTCGCAT CCTCGGTTTT CTGGAAGGCG AGCATCGTTT GTTCGCCCAG
     1921 GACTCTAGCT ATAGTTCTAG TGGTTGGCTA CTAATAGGTT GTATTGATGT TGGACGAGTC
     1981 GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT
     2041 CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA
     2101 TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA
     2161 CACTGGCAGA GCATTACGCT GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT
     2221 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT
     2281 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG
     2341 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA
     2401 GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA
     2461 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG
     2521 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG
     2581 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT
     2641 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA
     2701 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC
     2761 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG
     2821 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG
     2881 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT
     2941 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCTAGCCA GGAAGAGTTT GTAGAAACGC
     3001 AAAAAGGCCA TCCGTCAGGA TGGCCTTCTG CTTAGTTTGA TGCCTGGCAG TTTATGGCGG
     3061 GCGTCCTGCC CGCCACCCTC CGGGCCGTTG CTTCACAACG TTCAAATCCG CTCCCGGCGG
     3121 ATTTGTCCTA CTCAGGAGAG CGTTCACCGA CAAACAACAG ATAAAACGAA AGGCCCAGTC
     3181 TTCCGACTGA GCCTTTCGTT TTATTTGATG CCTGGCAGTT CCCTACTCTC GCGTTAACGC
     3241 TAGCATGGAT CTCGGGCCCC AAATAATGAT TTTATTTTGA CTGATAGTGA CCTGTTCGTT
     3301 GCAACAAATT GATGAGCAAT GCTTTTTTAT AATGCCAAGT TTGTACAAAA AAGCAGGCTT
     3361 ACTGGAAGTT CTGTTCCAGG GGCCCATGCA ACCAGACATG TCCTTGAATG TCATTAAGAT
     3421 GAAATCCAGT GACTTCCTGG AGAGTGCAGA ACTGGACAGC GGAGGCTTTG GGAAGGTGTC
     3481 TCTGTGTTTC CACAGAACCC AGGGACTCAT GATCATGAAA ACAGTGTACA AGGGGCCCAA
     3541 CTGCATTGAG CACAACGAGG CCCTCTTGGA GGAGGCGAAG ATGATGAACA GACTGAGACA
     3601 CAGCCGGGTG GTGAAGCTCC TGGGCGTCAT CATAGAGGAA GGGAAGTACT CCCTGGTGGC
     3661 GGAGTACATG GAGAAGGGCA ACCTGATGCA CGTGCTGAAA GCCGAGATGA GTACTCCGCT
     3721 TTCTGTAAAA GGAAGGATAA TTTTGGAAAT CATTGAAGGA ATGTGCTACT TACATGGAAA
     3781 AGGCGTGATA CACAAGGACC TGAAGCCTGA AAATATCCTT GTTGATAATG ACTTCCACAT
     3841 TAAGATCGCA GACCTCGGCC TTGCCTCCTT TAAGATGTGG AGCAAACTGA ATAATGAAGA
     3901 GCACAATGAG CTGAGGGAAG TGGACGGCAC CGCTAAGAAG AATGGCGGCA CCCTCTACTA
     3961 CATGGCGCCC GAGCACCTGA ATGACGTCAA CGCAAAGCCC ACAGAGAAGT CGGATGTGTA
     4021 CAGCTTTGCT GTAGTACTCT GGGCGATATT TGCAAATAAG GAGCCATATG AAAATGCTAT
     4081 CTGTGAGCAG CAGTTGATAA TGTGCATAAA ATCTGGGAAC AGGCCAGATG TGGATGACAT
     4141 CACTGAGTAC TGCCCAAGAG AAATTATCAG TCTCATGAAG CTCTGCTGGG AAGCGAATCC
     4201 GGAAGCTCGG CCGACATTTC CTGGCATTGA AGAAAAATTT AGGCCTTTTT ATTTAAGTCA
     4261 ATTAGAAGAA AGTGTAGAAG AGGACGTGAA GAGTTTAAAG AAAGAGTATT CAAACGAAAA
     4321 TGCAGTTGTG AAGAGAATGC AGTCTCTTCA ACTTGATTGT GTGGCAGTAC CTTCAAGCCG
     4381 GTCAAATTCA GCCACAGAAC AGCCTGGTTC ACTGCACAGT TCCCAGGGAC TTGGGATGGG
     4441 TCCTGTGGAG GAGTCCTGGT TTGCTCCTTC CCTGGAGCAC CCACAAGAAG AGAATGAGCC
     4501 CAGCCTGCAG AGTAAACTCC AAGACGAAGC CAACTACCAT CTTTATGGCA GCCGCATGGA
     4561 CAGGCAGACG AAACAGCAGC CCAGACAGAA TGTGGCTTAC AACAGAGAGG AGGAAAGGAG
     4621 ACGCAGGGTC TCCCATGACC CTTTTGCACA GCAAAGACCT TACGAGAATT TTCAGAATAC
     4681 AGAGGGAAAA GGCACTGCTT ATTCCAGTGC AGCCAGTCAT GGTAATGCAG TGCACCAGCC
     4741 CTCAGGGCTC ACCAGCCAAC CTCAAGTACT GTATCAGAAC AATGGATTAT ATAGCTCACA
     4801 TGGCTTTGGA ACAAGACCAC TGTAATACCC AGCTTTC
//
