LOCUS       pENTR3C-hSLC12A6        5597 bp    DNA     circular      7-JAN-2011
DEFINITION  LMBP 8643.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8643
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|578503629|
COMMENT     VNTDBDATE|578503728|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENTR3C-hSLC12A6|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 1532 0 1944 0 0 "") 
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COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 2 596 0 3028 0 0 "") 
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FEATURES             Location/Qualifiers
     terminator      complement(5374..5401)
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      complement(5199..5242)
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     rep_origin      4417..5090
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     CDS             3544..4353
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     misc_recomb     complement(3322..3421)
                     /invitrogen="2680000"
                     /vntifkey="86"
                     /label=attL2
     misc_recomb     5451..5550
                     /invitrogen="2670000"
                     /vntifkey="86"
                     /label=attL1
     CDS             1..3303
                     /vntifkey="4"
                     /label=hSLC12A6V3
BASE COUNT     1447 a      1286 c      1358 g      1506 t 
ORIGIN
        1 atgagtgaga tgtctggggc caccacttcg ctggcaactg ttgcactgga tccacccagt 
       61 gaccggactt ctcaccccca ggatgtcatc gaggacctga gtcagaactc catcacaggg 
      121 gaacacagcc aactgttaga cgacggacat aagaaagctc gaaatgctta tctcaataat 
      181 tccaattatg aagaaggaga tgaatatttt gataaaaatt tggcactctt tgaggaagaa 
      241 atggacacca gaccgaaggt gtcttccctc ctcaaccgca tggccaatta cactaatctg 
      301 actcaaggag caaaggaaca tgaagaggca gaaaacatca ctgaagggaa aaagaagccc 
      361 accaagaccc cccaaatggg taccttcatg ggtgtctacc tcccatgtct acaaaatatt 
      421 tttggagtga tccttttttt acgccttaca tgggtggtgg gcacagctgg agttcttcag 
      481 gcttttgcaa ttgtccttat ctgctgctgc tgtacaatgt tgactgctat ctccatgagt 
      541 gccattgcca ctaatggagt ggtgccagct gggggctcat actttatgat ttcccgggca 
      601 ctgggcccag agtttggtgg ggctgttggc ctctgctttt atcttggtac cacatttgca 
      661 gcagccatgt acatccttgg tgccattgaa atctttctgg tctatatcgt cccccgagct 
      721 gccatctttc acagtgatga cgcactcaag gaatcagcag ccatgctaaa taacatgcgt 
      781 gtctacggca cagctttctt ggtccttatg gtattagtgg tatttatcgg cgtacgctat 
      841 gtgaacaagt ttgcctcact tttcctggcc tgtgtcattg tgtccatctt ggccatctat 
      901 gctggagcca tcaagtcttc ttttgctcct ccacacttcc cggtctgcat gctgggtaac 
      961 cgcacccttt catcaagaca cattgacgtt tgctctaaga ccaaggaaat taacaacatg 
     1021 acagtcccat caaagttatg gggattcttc tgtaactcga gtcaattttt caatgccacc 
     1081 tgtgatgaat actttgttca caataacgtc acttcaatcc agggcattcc tggattggct 
     1141 agtggtataa ttacagagaa tctttggagt aattacctac ccaagggaga gatcatcgaa 
     1201 aagccttcag ccaaatcttc tgatgtctta ggcagcttaa accatgaata tgttcttgtt 
     1261 gacatcacca cctccttcac gcttctggtg ggaatcttct ttccctctgt tacaggtatc 
     1321 atggctggat caaacagatc tggagatctg aaagatgctc agaagtctat tccgattggt 
     1381 actatccttg ccatcctgac cacctccttt gttttagatt taagcaatgt tgtccttttt 
     1441 ggtgcatgta ttgaaggggt tgttctcaga gacaagttcg gtgatgctgt gaaaggtaat 
     1501 ttggtggtag gcaccttatc ttggccatcc ccatgggtga ttgttattgg ctccttcttt 
     1561 tcaacatgtg gggctggact tcagagcctc acaggtgcac cgaggctgct acaagctatt 
     1621 gccaaggata acatcatacc gtttctgagg gtttttggcc acagcaaagc caatggggaa 
     1681 cctacctggg ctttacttct aactgctgcc attgcagagc ttggaatact cattgcctcc 
     1741 ctggatcttg tggccccaat tctttccatg ttttttctca tgtgttacct ctttgtaaac 
     1801 ttggcatgtg ccttgcaaac attacttcga acacccaact ggagaccccg attccgctac 
     1861 taccattggg ccctttcttt catgggaatg agtatctgtc tggctctgat gttcatttct 
     1921 tcctggtatt atgccattgt agccatggta atagctggta tgatctacaa gtacattgaa 
     1981 taccaaggag ctgagaaaga atggggtgat ggtatccgtg ggctgtccct cagtgcagcc 
     2041 cggtttgctt tgcttcgatt ggaggaagga cctccacaca ctaaaaactg gaggcctcag 
     2101 ttgcttgtat tactgaaact agatgaagac ttacatgtca agcatcctcg cctcctcacc 
     2161 tttgcctcac agctcaaagc aggaaaaggt ctcactattg tgggctctgt catcgtgggg 
     2221 aacttcctag agaactacgg tgaagcttta gctgctgagc agaccataaa gcacctaatg 
     2281 gaggcagaga aggtaaaagg attctgccag ctggtggtgg ccgccaagct gagggagggc 
     2341 atttcccacc tcatccagtc atgtggcctt gggggcatga agcacaacac ggtggtgatg 
     2401 ggctggccta atggctggcg tcaaagcgaa gatgcccgcg cttggaagac ttttattggc 
     2461 acagttcgag tgacaactgc tgcccatctt gcactgctgg tggctaaaaa catctccttc 
     2521 tttcccagca atgtggagca attttctgag ggcaacattg atgtgtggtg gattgtgcat 
     2581 gatgggggga tgcttatgct actaccattc ctactgaaac agcacaaggt gtggcgaaag 
     2641 tgcagcatac ggatcttcac agtagcccaa ttagaagaca acagtatcca aatgaagaag 
     2701 gacctagcca ccttcctata tcacttacgc attgaggcgg aggtagaagt ggtggagatg 
     2761 gtgagaaagc tgagtttgag atacaagagc atgacagtga tatatcagca tatacttacg 
     2821 agcgcacttt gatgatggaa caaaggtccc agatgcttcg gcacatgcgg ctatccaaaa 
     2881 cagagcgaga cagagaggca caattggtga aagaccgaaa ctcaatgcta cgattgacca 
     2941 gcattggctc tgatgaggac gaagagacag aaacctatca ggagaaggtg cacatgactt 
     3001 ggacaaaaga caagtacatg gcatcccggg gacaaaaagc gaagtcaatg gaaggattcc 
     3061 aggacctgct taacatgcgt ccggaccagt ccaatgtgag gcggatgcat acagcagtga 
     3121 aactcaacga ggttatagtt aacaagtccc atgaagcaaa gctggtttta ttgaatatgc 
     3181 cagggccacc ccgaaaccct gagggtgatg aaaactacat ggagttccta gaggtgctta 
     3241 ccgagggact agagcgagtc ctacttgtcc ggggtggtgg cagtgaagtg atcaccattt 
     3301 attcacactc gagatatcta gacccagctt tcttgtacaa agttggcatt ataagaaagc 
     3361 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt 
     3421 gccatccagc tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat 
     3481 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt 
     3541 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct 
     3601 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat 
     3661 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt 
     3721 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt 
     3781 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc 
     3841 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt 
     3901 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt 
     3961 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt 
     4021 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa 
     4081 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt 
     4141 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga 
     4201 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct 
     4261 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg 
     4321 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacat 
     4381 tattcagatt gggccccgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 
     4441 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 
     4501 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 
     4561 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 
     4621 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 
     4681 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 
     4741 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 
     4801 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 
     4861 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 
     4921 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 
     4981 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 
     5041 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 
     5101 gcgttatccc ctgattctgt ggataaccgt attaccgcta gcatggatct cggggacgtc 
     5161 taactactaa gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcgg 
     5221 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa 
     5281 atccgccggg agcggatttg aacgttgtga agcaacggcc cggagggtgg cgggcaggac 
     5341 gcccgccata aactgccagg catcaaacta agcagaaggc catcctgacg gatggccttt 
     5401 ttgcgtttct acaaactctt cctgttagtt agttacttaa gctcgggccc caaataatga 
     5461 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa tgctttttta 
     5521 taatgccaac tttgtacaaa aaagcaggct ctttaaagga accaattcag tcgactggat 
     5581 ccggtaccga attcacc 
//
