LOCUS       pENTR207-PRS-mRIP1-K45R(KD+ID)      4822 bp    DNA     circular UNA 10-FEB-2012
DEFINITION  LMBP 8644.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8644
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
FEATURES             Location/Qualifiers
     CDS             1..1425
                     /label="mRIP1K45R (KD + ID) + stop"
     Misc._feature   complement(1430..1525)
                     /label=attL2
     Primer          complement(1550..1573)
                     /invitrogen="1994000"
                     /label="pDONR207 reverse primer"
     CDS             complement(2309..2842)
                     /invitrogen="1150000"
                     /label=Gm(R)
     Promoter        complement(3031..3058)
                     /invitrogen="2250000"
                     /label="Pc promoter"
     Terminator      4439..4466
                     /invitrogen="2590000"
                     /label="rrnB T2 transcription terminator"
     Terminator      4598..4641
                     /invitrogen="2580000"
                     /label="rrnB T1 transcription terminator"
     Primer          4666..4690
                     /invitrogen="1993000"
                     /label="pDONR207 forward primer"
     Misc._feature   4698..4793
                     /label=attL1
     Cleavage_site   4799..4822
                     /label="PRS (prescission cleavage site)"
ORIGIN
        1 ATGCAACCAG ACATGTCCTT GGACAATATT AAGATGGCAT CCAGTGACCT GCTGGAGAAG
       61 ACAGACCTAG ACAGCGGAGG CTTCGGGAAG GTGTCCTTGT GTTACCACAG AAGCCATGGA
      121 TTTGTCATCC TGAGAAAAGT ATACACAGGG CCCAACCGCG CTGAGTACAA TGAGGTTCTC
      181 TTGGAAGAGG GGAAGATGAT GCACAGACTG AGACACAGTC GAGTGGTGAA GCTACTGGGC
      241 ATCATCATAG AAGAAGGGAA CTATTCGCTG GTGATGGAGT ACATGGAGAA GGGCAACCTG
      301 ATGCACGTGC TAAAGACCCA GATAGATGTC CCACTTTCAT TGAAAGGAAG GATAATCGTG
      361 GAGGCCATAG AAGGCATGTG CTACTTACAT GACAAAGGTG TGATACACAA GGACCTGAAG
      421 CCTGAGAATA TCCTCGTTGA TCGTGACTTT CACATTAAGA TAGCCGATCT TGGTGTGGCT
      481 TCCTTTAAGA CATGGAGCAA ACTGACTAAG GAGAAAGACA ACAAGCAGAA AGAAGTGAGC
      541 AGCACCACTA AGAAGAACAA TGGTGGTACC CTTTACTACA TGGCACCCGA ACACCTGAAT
      601 GACATCAATG CAAAGCCCAC GGAGAAGTCG GACGTGTACA GCTTTGGCAT TGTCCTTTGG
      661 GCAATATTTG CAAAAAAGGA GCCCTATGAG AATGTCATCT GTACTGAGCA GTTCGTGATC
      721 TGCATAAAAT CTGGGAACAG GCCAAATGTA GAGGAAATCC TTGAGTACTG TCCAAGGGAG
      781 ATCATCAGCC TCATGGAGCG GTGCTGGCAG GCGATCCCAG AAGACAGGCC AACATTTCTT
      841 GGCATTGAAG AAGAATTTAG GCCTTTTTAC TTAAGTCATT TTGAAGAATA TGTAGAAGAG
      901 GATGTGGCAA GTTTAAAGAA AGAGTATCCA GATCAAAGCC CAGTGCTGCA GAGAATGTTT
      961 TCACTGCAGC ATGACTGTGT ACCCTTACCT CCGAGCAGGT CAAATTCAGA ACAACCTGGA
     1021 TCGCTGCACA GTTCCCAGGG GCTCCAGATG GGTCCTGTGG AGGAGTCCTG GTTTTCTTCC
     1081 TCCCCAGAGT ACCCACAGGA CGAGAATGAT CGCAGTGTGC AGGCTAAGCT GCAAGAGGAA
     1141 GCCAGCTATC ATGCTTTTGG AATATTTGCA GAGAAACAGA CAAAACCGCA GCCAAGGCAG
     1201 AATGAGGCTT ACAACAGAGA GGAGGAAAGG AAACGAAGGG TCTCTCATGA CCCCTTTGCA
     1261 CAGCAGAGAG CTCGTGAGAA TATTAAGAGT GCAGGAGCAA GAGGTCATTC TGATCCCAGC
     1321 ACAACGAGTC GTGGAATTGC AGTGCAACAG CTGTCATGGC CAGCCACCCA AACAGTTTGG
     1381 AACAATGGAT TGTATAATCA GCATGGATTT GGAACTACAG GTTAATACCC AGCTTTCTTG
     1441 TACAAAGTGG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT
     1501 ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGCAG CTCTGGCCCG TGTCTCAAAA
     1561 TCTCTGATGT TACATTGCAC AAGATAAAAA TATATCATCA TGAACAATAA AACTGTCTGC
     1621 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC
     1681 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT
     1741 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT
     1801 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA
     1861 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA
     1921 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA
     1981 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC
     2041 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA
     2101 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG
     2161 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT
     2221 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAGGCCG
     2281 GGAAGCCGAT CTCGGCTTGA ACGAATTGTT AGGTGGCGGT ACTTGGGTCG ATATCAAAGT
     2341 GCATCACTTC TTCCCGTATG CCCAACTTTG TATAGAGAGC CACTGCGGGA TCGTCACCGT
     2401 AATCTGCTTG CACGTAGATC ACATAAGCAC CAAGCGCGTT GGCCTCATGC TTGAGGAGAT
     2461 TGATGAGCGC GGTGGCAATG CCCTGCCTCC GGTGCTCGCC GGAGACTGCG AGATCATAGA
     2521 TATAGATCTC ACTACGCGGC TGCTCAAACC TGGGCAGAAC GTAAGCCGCG AGAGCGCCAA
     2581 CAACCGCTTC TTGGTCGAAG GCAGCAAGCG CGATGAATGT CTTACTACGG AGCAAGTTCC
     2641 CGAGGTAATC GGAGTCCGGC TGATGTTGGG AGTAGGTGGC TACGTCTCCG AACTCACGAC
     2701 CGAAAAGATC AAGAGCAGCC CGCATGGATT TGACTTGGTC AGGGCCGAGC CTACATGTGC
     2761 GAATGATGCC CATACTTGAG CCACCTAACT TTGTTTTAGG GCGACTGCCC TGCTGCGTAA
     2821 CATCGTTGCT GCTGCGTAAC ATCGTTGCTG CTCCATAACA TCAAACATCG ACCCACGGCG
     2881 TAACGCGCTT GCTGCTTGGA TGCCCGAGGC ATAGACTGTA CAAAAAAACA GTCATAACAA
     2941 GCCATGAAAA CCGCCACTGC GCCGTTACCA CCGCTGCGTT CGGTCAAGGT TCTGGACCAG
     3001 TTGCGTGAGC GCATACGCTA CTTGCATTAC AGTTTACGAA CCGAACAGGC TTATGTCAAC
     3061 TGGGTTCGTG CCTTCATCCG TTTCCACGGT GTGCGTCACC CGGCAACCTT GGGCAGCAGC
     3121 GAAGTCGAGG CATTTCTGTC CTGGCTGGCG AACGAGCGCA AGGTTTCGGT CTCCACGCAT
     3181 CGTCAGGCAT TGGCGGCCTT GCTGTTCTTC TACGGCAAGG TGCTGTGCAC GGATCTGCCC
     3241 TGGCTTCAGG AGATCGGAAG ACCTCGGCCG TCGCGGCGCT TGCCGGTGGT GCTGACCCCG
     3301 GATGAAGTGG TTCGCATCCT CGGTTTTCTG GAAGGCGAGC ATCGTTTGTT CGCCCAGGAC
     3361 TCTAGCTATA GTTCTAGTGG TTGGCTACTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA
     3421 ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT
     3481 TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG
     3541 CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG GTTGTAACAC
     3601 TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA ATCCCTTAAC
     3661 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG
     3721 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG
     3781 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA
     3841 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
     3901 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA
     3961 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC
     4021 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA
     4081 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA
     4141 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC
     4201 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC
     4261 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG
     4321 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT
     4381 CCCCTGATTC TGTGGATAAC CGTATTACCG CTAGCCAGGA AGAGTTTGTA GAAACGCAAA
     4441 AAGGCCATCC GTCAGGATGG CCTTCTGCTT AGTTTGATGC CTGGCAGTTT ATGGCGGGCG
     4501 TCCTGCCCGC CACCCTCCGG GCCGTTGCTT CACAACGTTC AAATCCGCTC CCGGCGGATT
     4561 TGTCCTACTC AGGAGAGCGT TCACCGACAA ACAACAGATA AAACGAAAGG CCCAGTCTTC
     4621 CGACTGAGCC TTTCGTTTTA TTTGATGCCT GGCAGTTCCC TACTCTCGCG TTAACGCTAG
     4681 CATGGATCTC GGGCCCCAAA TAATGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA
     4741 ACAAATTGAT GAGCAATGCT TTTTTATAAT GCCAAGTTTG TACAAAAAAG CAGGCTTACT
     4801 GGAAGTTCTG TTCCAGGGGC CC
//
