LOCUS       pENTR207-PRS-mRIP1-K45R        5368 bp    DNA     circular     18-JAN-2011
DEFINITION  LMBP 8650.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8650
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|579451881|
COMMENT     VNTDBDATE|579451881|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENTR207-PRS-mRIP1-K45R|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pENTR207-PRS-mRIP1-K45R" 0 5368 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
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COMMENT             (CTerm 2 0 2657 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 414 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 2081894036 0 "" 0 "" "" "" "" "" "" 
COMMENT             "mRIP1K45R FL fragment met primers" "" 0 0 1 2025 3344 5368 14 2038 
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COMMENT             (CTerm 2 0 14 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 2038 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
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COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #2=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 2 1789 0 2610 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 3 1203 0 1467 0 3252 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 5176 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 5006 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 3500 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 103 0 4336 0 4354 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "")) 
COMMENT       (CObList 
COMMENT        #12=(CFSignal (CObList) "attL1" 21 0 0 3261 3356 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #13=(CFSignal (CObList) "attL2" 21 0 1 5361 88 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "pDONR\201E07 reverse primer" 27 0 1 113 136 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"1994000\"") 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "pDONR\201E07 forward primer" 27 0 0 3229 3253 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"1993000\"") 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "rrnB T2 transcription terminator" 43 0 0 3002 
COMMENT             3029 1 (CStringList) (CStringList "/invitrogen=\"2590000\"") 1 1 1 
COMMENT             1 "") 
COMMENT        #17=(CFSignal (CObList) "rrnB T1 transcription terminator" 43 0 0 3161 
COMMENT             3204 1 (CStringList) (CStringList "/invitrogen=\"2580000\"") 1 1 1 
COMMENT             1 "") 
COMMENT        #18=(CFSignal (CObList) "Pc promoter" 29 0 1 1594 1621 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2250000\"") 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "Gm(R)" 4 0 1 872 1405 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1150000\"") 1 1 1 1 "")) (CObList) 
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COMMENT                          "DNA 'pENTR207-PRS-mRIP1-K45R'" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "attL1-attL2" 1) 
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COMMENT                          "Local object" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 01/18/11 02:51" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/18/11 02:51" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5368 bp" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Constructed" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                   "Standard Fields") 
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COMMENT                   "Annotations") 
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COMMENT                          "If necessary, linearize attB sequence mRIP1K45R FL fragment met primers at a unique REN site outside the attB region. Ethanol precipitate and resuspend." 
COMMENT                          1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Mix linearized attB sequence mRIP1K45R FL fragment met primers and pDONR Vector pDONR207 in BP buffer and TE buffers." 
COMMENT                          1) 
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COMMENT                          "Thaw, vortex and add BP Clonase enzyme. Incubate >= 1 hr. at 25\260." 
COMMENT                          1) 
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COMMENT                          "Add Proteinase K for 10 min at 37\260." 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Transform into DH5a or TOP10 E. coli or any non-F' episome bearing strain." 
COMMENT                          1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Select Entry Clones on agar plates with antibiotic gentamicin." 
COMMENT                          1))) "Design Description") 
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COMMENT                                        " N3: 3252 " 1))))) "AvaI: 3 sites") 1 5 
COMMENT                          "CYCGRG" "GRGCYC") 
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COMMENT                          "ATCGAT" "TAGCTA") 
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COMMENT                          "CCATGG" "GGTACC") 
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COMMENT                          "CTGCAG" "GACGTC") 
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COMMENT              #68=(CIvgnResourcePar 
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COMMENT                    "<a href=\"ivgnlink:/iq\">Invitrogen products related to pENTR207-PRS-mRIP1-K45R</a>" 
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COMMENT                            #73=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 3343" 1) 
COMMENT                            #74=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 1 to 3343" 1) 
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COMMENT                                        "  Position 414" 1))) "Right Terminus"))) 
COMMENT                          "#1: FRAGMENT of <a href=\"vnti:dnarna/pDONR207?sel=2657-414\">pDONR207</a> ") 
COMMENT                     #79=(CFolderPar 
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COMMENT                                 "parent position: from 14 to 2038" 1) 
COMMENT                            #81=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 2025" 1) 
COMMENT                            #82=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 3344 to 5368" 1) 
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COMMENT                          "#2: FRAGMENT of <a href=\"vnti:dnarna/mRIP1K45R%20FL%20fragment%20met%20primers?sel=14-2038\">mRIP1K45R FL fragment met primers</a> "))) 
COMMENT                   "Component Fragments") 
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COMMENT                                        "Start: 872   End: 1405 (Complementary)" 
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COMMENT                          "Misc. Feature (2 total)") 
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COMMENT                                           "Start: 1594  End: 1621 (Complementary)" 
COMMENT                                           1))) "Pc promoter"))) 
COMMENT                           "Promoter Eukaryotic (1 total)") 
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COMMENT                                           "Start: 3002  End: 3029" 1))) 
COMMENT                                   "rrnB T2 transcription terminator") 
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COMMENT                           "Terminator (2 total)"))) "Feature Map")))) 
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FEATURES             Location/Qualifiers
     misc_feature    3261..3356
                     /vntifkey="21"
                     /label=attL1
     misc_feature    complement(5361..88)
                     /vntifkey="21"
                     /label=attL2
     primer          complement(113..136)
                     /invitrogen="1994000"
                     /vntifkey="27"
                     /label=pDONR207\reverse\primer
     primer          3229..3253
                     /invitrogen="1993000"
                     /vntifkey="27"
                     /label=pDONR207\forward\primer
     terminator      3002..3029
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      3161..3204
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     promoter        complement(1594..1621)
                     /invitrogen="2250000"
                     /vntifkey="29"
                     /label=Pc\promoter
     CDS             complement(872..1405)
                     /invitrogen="1150000"
                     /vntifkey="4"
                     /label=Gm(R)
BASE COUNT     1438 a      1260 c      1368 g      1302 t 
ORIGIN
        1 ttgtacaaag tgggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc 
       61 actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca 
      121 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc 
      181 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag 
      241 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa 
      301 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt 
      361 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact 
      421 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga 
      481 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga 
      541 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca 
      601 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc 
      661 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg 
      721 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc 
      781 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattagg 
      841 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa 
      901 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac 
      961 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga 
     1021 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat 
     1081 agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc 
     1141 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt 
     1201 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac 
     1261 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg 
     1321 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg 
     1381 taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg 
     1441 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa 
     1501 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac 
     1561 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc 
     1621 aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc 
     1681 agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg 
     1741 catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg 
     1801 ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc 
     1861 ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag 
     1921 gactctagct atagttctag tggttggcta ctaataggtt gtattgatgt tggacgagtc 
     1981 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct 
     2041 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa 
     2101 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa 
     2161 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt 
     2221 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 
     2281 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 
     2341 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 
     2401 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 
     2461 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 
     2521 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 
     2581 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 
     2641 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 
     2701 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 
     2761 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 
     2821 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 
     2881 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 
     2941 tatcccctga ttctgtggat aaccgtatta ccgctagcca ggaagagttt gtagaaacgc 
     3001 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg 
     3061 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg 
     3121 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc 
     3181 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc 
     3241 tagcatggat ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt 
     3301 gcaacaaatt gatgagcaat gcttttttat aatgccaagt ttgtacaaaa aagcaggctt 
     3361 actggaagtt ctgttccagg ggcccatgca accagacatg tccttggaca atattaagat 
     3421 ggcatccagt gacctgctgg agaagacaga cctagacagc ggaggcttcg ggaaggtgtc 
     3481 cttgtgttac cacagaagcc atggatttgt catcctgaga aaagtataca cagggcccaa 
     3541 ccgcgctgag tacaatgagg ttctcttgga agaggggaag atgatgcaca gactgagaca 
     3601 cagtcgagtg gtgaagctac tgggcatcat catagaagaa gggaactatt cgctggtgat 
     3661 ggagtacatg gagaagggca acctgatgca cgtgctaaag acccagatag atgtcccact 
     3721 ttcattgaaa ggaaggataa tcgtggaggc catagaaggc atgtgctact tacatgacaa 
     3781 aggtgtgata cacaaggacc tgaagcctga gaatatcctc gttgatcgtg actttcacat 
     3841 taagatagcc gatcttggtg tggcttcctt taagacatgg agcaaactga ctaaggagaa 
     3901 agacaacaag cagaaagaag tgagcagcac cactaagaag aacaatggtg gtacccttta 
     3961 ctacatggca cccgaacacc tgaatgacat caatgcaaag cccacggaga agtcggacgt 
     4021 gtacagcttt ggcattgtcc tttgggcaat atttgcaaaa aaggagccct atgagaatgt 
     4081 catctgtact gagcagttcg tgatctgcat aaaatctggg aacaggccaa atgtagagga 
     4141 aatccttgag tactgtccaa gggagatcat cagcctcatg gagcggtgct ggcaggcgat 
     4201 cccagaagac aggccaacat ttcttggcat tgaagaagaa tttaggcctt tttacttaag 
     4261 tcattttgaa gaatatgtag aagaggatgt ggcaagttta aagaaagagt atccagatca 
     4321 aagcccagtg ctgcagagaa tgttttcact gcagcatgac tgtgtaccct tacctccgag 
     4381 caggtcaaat tcagaacaac ctggatcgct gcacagttcc caggggctcc agatgggtcc 
     4441 tgtggaggag tcctggtttt cttcctcccc agagtaccca caggacgaga atgatcgcag 
     4501 tgtgcaggct aagctgcaag aggaagccag ctatcatgct tttggaatat ttgcagagaa 
     4561 acagacaaaa ccgcagccaa ggcagaatga ggcttacaac agagaggagg aaaggaaacg 
     4621 aagggtctct catgacccct ttgcacagca gagagctcgt gagaatatta agagtgcagg 
     4681 agcaagaggt cattctgatc ccagcacaac gagtcgtgga attgcagtgc aacagctgtc 
     4741 atggccagcc acccaaacag tttggaacaa tggattgtat aatcagcatg gatttggaac 
     4801 tacaggtaca ggagtttggt atccgccaaa tctaagccaa atgtatagta cttataaaac 
     4861 tccagtgcca gagaccaaca taccgggaag cacacccacc atgccatact tctctgggcc 
     4921 agtagcagat gacctcataa aatatactat attcaatagt tctggtattc agattggaaa 
     4981 ccacaattat atggatgttg gactgaattc acaaccacca aacaatactt gcaaagaaga 
     5041 gtcgacttcc agacaccaag ccatctttga taacaccact agtctgactg atgaacacct 
     5101 gaaccctatc agggaaaacc tgggaaggca gtggaaaaac tgtgcccgca agctgggctt 
     5161 cactgagtct cagatcgatg aaatcgacca tgactatgaa agagatggac tgaaagagaa 
     5221 agtttaccaa atgcttcaga agtggctgat gcgggaaggc accaaagggg ccacagtggg 
     5281 aaagttggcc caggcacttc accaatgttg caggatagac ctgctgaacc acttgattcg 
     5341 tgccagccag agctaatacc cagctttc 
//
