LOCUS       pENTR207-PRS-hRIP3-K50A        4954 bp    DNA     circular     16-FEB-2011
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|582028870|
COMMENT     VNTDBDATE|582028870|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENTR207-PRS-hRIP3-K50A|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "If necessary, linearize attB sequence fragment hRIP3 K50A met primers at a unique REN site outside the attB region. Ethanol precipitate and resuspend." 
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COMMENT                          "Thaw, vortex and add BP Clonase enzyme. Incubate >= 1 hr. at 25\260." 
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COMMENT                          "Add Proteinase K for 10 min at 37\260." 1) 
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FEATURES             Location/Qualifiers
     misc_feature    3261..3356
                     /vntifkey="21"
                     /label=attL1
     misc_feature    complement(4947..88)
                     /vntifkey="21"
                     /label=attL2
     primer          complement(113..136)
                     /invitrogen="1994000"
                     /vntifkey="27"
                     /label=pDONR�E07\reverse\primer
     primer          3229..3253
                     /invitrogen="1993000"
                     /vntifkey="27"
                     /label=pDONR�E07\forward\primer
     terminator      3002..3029
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      3161..3204
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     promoter        complement(1594..1621)
                     /invitrogen="2250000"
                     /vntifkey="29"
                     /label=Pc\promoter
     CDS             complement(872..1405)
                     /invitrogen="1150000"
                     /vntifkey="4"
                     /label=Gm(R)
BASE COUNT     1210 a      1256 c      1315 g      1173 t 
ORIGIN
        1 ttgtacaaag tgggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc 
       61 actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca 
      121 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc 
      181 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag 
      241 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa 
      301 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt 
      361 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact 
      421 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga 
      481 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga 
      541 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca 
      601 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc 
      661 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg 
      721 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc 
      781 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattagg 
      841 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa 
      901 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac 
      961 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga 
     1021 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat 
     1081 agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc 
     1141 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt 
     1201 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac 
     1261 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg 
     1321 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg 
     1381 taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg 
     1441 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa 
     1501 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac 
     1561 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc 
     1621 aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc 
     1681 agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg 
     1741 catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg 
     1801 ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc 
     1861 ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag 
     1921 gactctagct atagttctag tggttggcta ctaataggtt gtattgatgt tggacgagtc 
     1981 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct 
     2041 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa 
     2101 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa 
     2161 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt 
     2221 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 
     2281 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 
     2341 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 
     2401 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 
     2461 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 
     2521 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 
     2581 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 
     2641 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 
     2701 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 
     2761 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 
     2821 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 
     2881 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 
     2941 tatcccctga ttctgtggat aaccgtatta ccgctagcca ggaagagttt gtagaaacgc 
     3001 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg 
     3061 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg 
     3121 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc 
     3181 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc 
     3241 tagcatggat ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt 
     3301 gcaacaaatt gatgagcaat gcttttttat aatgccaagt ttgtacaaaa aagcaggctt 
     3361 actggaagtt ctgttccagg ggcccatgtc gtgcgtcaag ttatggccca gcggtgcccc 
     3421 cgcccccttg gtgtccatcg aggaactgga gaaccaggag ctcgtcggca aaggcgggtt 
     3481 cggcacagtg ttccgggcgc aacataggaa gtggggctac gatgtggcgg tcgcgatcgt 
     3541 aaactcgaag gcgatatcca gggaggtcaa ggccatggca agtctggata acgaattcgt 
     3601 gctgcgccta gaaggggtta tcgagaaggt gaactgggac caagatccca agccagctct 
     3661 ggtgactaaa ttcatggaga acggctcctt gtcggggctg ctgcagtccc agtgccctcg 
     3721 gccctggccg ctcctttgcc gcctgctgaa agaagtggtg cttgggatgt tttacctgca 
     3781 cgaccagaac ccggtgctcc tgcaccggga cctcaagcca tccaacgtcc tgctggaccc 
     3841 agagctgcac gtcaagctgg cagattttgg cctgtccaca tttcagggag gctcacagtc 
     3901 agggacaggg tccggggagc cagggggcac cctgggctac ttggccccag aactgtttgt 
     3961 taacgtaaac cggaaggcct ccacagccag tgacgtctac agcttcggga tcctaatgtg 
     4021 ggcagtgctt gctggaagag aagttgagtt gccaaccgaa ccatcactcg tgtacgaagc 
     4081 agtgtgcaac aggcagaacc ggccttcatt ggctgagctg ccccaagccg ggcctgagac 
     4141 tcccggctta gaaggactga aggagctaat gcagctctgc tggagcagtg agcccaagga 
     4201 cagaccctcc ttccaggaat gcctaccaaa aactgatgaa gtcttccaga tggtggagaa 
     4261 caatatgaat gctgctgtct ccacggtaaa ggatttcctg tctcagctca ggagcagcaa 
     4321 taggagattt tctatcccag agtcaggcca aggagggaca gaaatggatg gctttaggag 
     4381 aaccatagaa aaccagcact ctcgtaatga tgtcatggtt tctgagtggc taaacaaact 
     4441 gaatctagag gagcctccca gctctgttcc taaaaaatgc ccgagcctta ccaagaggag 
     4501 cagggcacaa gaggagcagg ttccacaagc ctggacagca ggcacatctt cagattcgat 
     4561 ggcccaacct ccccagactc cagagacctc aactttcaga aaccagatgc ccagccctac 
     4621 ctcaactgga acaccaagtc ctggaccccg agggaatcag ggggctgaga gacaaggcat 
     4681 gaactggtcc tgcaggaccc cggagccaaa tccagtaaca gggcgaccgc tcgttaacat 
     4741 atacaactgc tctggggtgc aagttggaga caacaactac ttgactatgc aacagacaac 
     4801 tgccttgccc acatggggct tggcaccttc gggcaagggg aggggcttgc agcacccccc 
     4861 accagtaggt tcgcaagaag gccctaaaga tcctgaagcc tggagcaggc cacagggttg 
     4921 gtataatcat agcgggaaat aatacccagc tttc 
//
