LOCUS       pENTR207-PRS-hRIP1(KD+RHIM)        5116 bp    DNA     circular     28-FEB-2011
DEFINITION  LMBP 8656.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8656
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|583075131|
COMMENT     VNTDBDATE|583075131|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENTR207-PRS-hRIP1(KD+RHIM)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     CDS             complement(2603..3136)
                     /invitrogen="1150000"
                     /vntifkey="4"
                     /label=Gm(R)
     promoter        complement(3325..3352)
                     /invitrogen="2250000"
                     /vntifkey="29"
                     /label=Pc\promoter
     terminator      4892..4935
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     terminator      4733..4760
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     primer          4960..4984
                     /invitrogen="1993000"
                     /vntifkey="27"
                     /label=pDONRT207\forward\primer
     primer          complement(1844..1867)
                     /invitrogen="1994000"
                     /vntifkey="27"
                     /label=pDONRT207\reverse\primer
     misc_feature    complement(1724..1819)
                     /vntifkey="21"
                     /label=attL2
     misc_feature    4992..5087
                     /vntifkey="21"
                     /label=attL1
     CDS             1..1719
                     /vntifkey="4"
                     /label=hRIP1\-\DD
BASE COUNT     1353 a      1213 c      1301 g      1249 t 
ORIGIN
        1 atgcaaccag acatgtcctt gaatgtcatt aagatgaaat ccagtgactt cctggagagt 
       61 gcagaactgg acagcggagg ctttgggaag gtgtctctgt gtttccacag aacccaggga 
      121 ctcatgatca tgaaaacagt gtacaagggg cccaactgca ttgagcacaa cgaggccctc 
      181 ttggaggagg cgaagatgat gaacagactg agacacagcc gggtggtgaa gctcctgggc 
      241 gtcatcatag aggaagggaa gtactccctg gtgatggagt acatggagaa gggcaacctg 
      301 atgcacgtgc tgaaagccga gatgagtact ccgctttctg taaaaggaag gataattttg 
      361 gaaatcattg aaggaatgtg ctacttacat ggaaaaggcg tgatacacaa ggacctgaag 
      421 cctgaaaata tccttgttga taatgacttc cacattaaga tcgcagacct cggccttgcc 
      481 tcctttaaga tgtggagcaa actgaataat gaagagcaca atgagctgag ggaagtggac 
      541 ggcaccgcta agaagaatgg cggcaccctc tactacatgg cgcccgagca cctgaatgac 
      601 gtcaacgcaa agcccacaga gaagtcggat gtgtacagct ttgctgtagt actctgggcg 
      661 atatttgcaa ataaggagcc atatgaaaat gctatctgtg agcagcagtt gataatgtgc 
      721 ataaaatctg ggaacaggcc agatgtggat gacatcactg agtactgccc aagagaaatt 
      781 atcagtctca tgaagctctg ctgggaagcg aatccggaag ctcggccgac atttcctggc 
      841 attgaagaaa aatttaggcc tttttattta agtcaattag aagaaagtgt agaagaggac 
      901 gtgaagagtt taaagaaaga gtattcaaac gaaaatgcag ttgtgaagag aatgcagtct 
      961 cttcaacttg attgtgtggc agtaccttca agccggtcaa attcagccac agaacagcct 
     1021 ggttcactgc acagttccca gggacttggg atgggtcctg tggaggagtc ctggtttgct 
     1081 ccttccctgg agcacccaca agaagagaat gagcccagcc tgcagagtaa actccaagac 
     1141 gaagccaact accatcttta tggcagccgc atggacaggc agacgaaaca gcagcccaga 
     1201 cagaatgtgg cttacaacag agaggaggaa aggagacgca gggtctccca tgaccctttt 
     1261 gcacagcaaa gaccttacga gaattttcag aatacagagg gaaaaggcac tgcttattcc 
     1321 agtgcagcca gtcatggtaa tgcagtgcac cagccctcag ggctcaccag ccaacctcaa 
     1381 gtactgtatc agaacaatgg attatatagc tcacatggct ttggaacaag accactggat 
     1441 ccaggaacag caggtcccag agtttggtac aggccaattc caagtcatat gcctagtctg 
     1501 cataatatcc cagtgcctga gaccaactat ctaggaaata cacccaccat gccattcagc 
     1561 tccttgccac caacagatga atctataaaa tataccatat acaatagtac tggcattcag 
     1621 attggagcct acaattatat ggagattggt gggacgagtt catcactact agacagcaca 
     1681 aatacgaact tcaaagaaga gccagctgct aagtactaat acccagcttt cttgtacaaa 
     1741 gtgggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt 
     1801 caaaataaaa tcattatttg ccatccagct gcagctctgg cccgtgtctc aaaatctctg 
     1861 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat 
     1921 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt 
     1981 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca 
     2041 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa 
     2101 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct 
     2161 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg 
     2221 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga 
     2281 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc 
     2341 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg 
     2401 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt 
     2461 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac 
     2521 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattag gccgggaagc 
     2581 cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca 
     2641 cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg 
     2701 cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg agattgatga 
     2761 gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca tagatataga 
     2821 tctcactacg cggctgctca aacctgggca gaacgtaagc cgcgagagcg ccaacaaccg 
     2881 cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt 
     2941 aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa 
     3001 gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga 
     3061 tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt 
     3121 tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc 
     3181 gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg 
     3241 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt 
     3301 gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt 
     3361 cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc 
     3421 gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag 
     3481 gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt 
     3541 caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa 
     3601 gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca ggactctagc 
     3661 tatagttcta gtggttggct actaataggt tgtattgatg ttggacgagt cggaatcgca 
     3721 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta 
     3781 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt 
     3841 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag 
     3901 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt 
     3961 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 
     4021 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 
     4081 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 
     4141 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 
     4201 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 
     4261 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 
     4321 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 
     4381 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 
     4441 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 
     4501 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 
     4561 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 
     4621 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 
     4681 attctgtgga taaccgtatt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc 
     4741 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc 
     4801 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct 
     4861 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg 
     4921 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga 
     4981 tctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat 
     5041 tgatgagcaa tgctttttta taatgccaag tttgtacaaa aaagcaggct tactggaagt 
     5101 tctgttccag gggccc 
//
