LOCUS       pENTR207-PRS-hRIP1-D138N      5413 bp    DNA     circular UNA 10-FEB-2012
DEFINITION  LMBP 8662.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8662
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
FEATURES             Location/Qualifiers
     CDS             1..2016
                     /label="hRIP1D138N (FL) + stop"
     Misc._feature   complement(2021..2116)
                     /label=attL2
     Unsure          2028
                     /Design_Description="If necessary, linearize attB sequence
                     hRIP1D138N (FL) fragment + primers at a unique REN site
                     outside the attB region. Ethanol precipitate and
                     resuspend.	Mix linearized attB sequence hRIP1D138N (FL)
                     fragment + primers and pDONR Vector pDONR207 in BP buffer
                     and TE buffers.	Thaw, vortex and add BP Clonase enzyme.
                     Incubate >= 1 hr. at 25°.	Add Proteinase K for 10 min at
                     37°.	Transform into DH5a or TOP10 E. coli or any non-F'
                     episome bearing strain.	Select Entry Clones on agar plates
                     with antibiotic gentamicin."
                     /label="Gateway Cloning Description"
     Insertion       2029..5371
                     /note="Region on donor sequence (pDONR207): 2657..414"
                     /label=pDONR207
     Primer          complement(2141..2164)
                     /invitrogen="1994000"
                     /label="pDONR?E07 reverse primer"
     CDS             complement(2900..3433)
                     /invitrogen="1150000"
                     /label=Gm(R)
     Promoter        complement(3622..3649)
                     /invitrogen="2250000"
                     /label="Pc promoter"
     Terminator      5030..5057
                     /invitrogen="2590000"
                     /label="rrnB T2 transcription terminator"
     Terminator      5189..5232
                     /invitrogen="2580000"
                     /label="rrnB T1 transcription terminator"
     Primer          5257..5281
                     /invitrogen="1993000"
                     /label="pDONR?E07 forward primer"
     Misc._feature   5289..5384
                     /label=attL1
     Insertion       5372..>5413
                     /note="Region on donor sequence (hRIP1D138N (FL) fragment
                     + primers): 14..2083"
                     /label="hRIP1D138N (FL) fragment + primers"
     Cleavage_site   5390..5413
                     /label="prescission cleavage site ( PRS)"
ORIGIN
        1 ATGCAACCAG ACATGTCCTT GAATGTCATT AAGATGAAAT CCAGTGACTT CCTGGAGAGT
       61 GCAGAACTGG ACAGCGGAGG CTTTGGGAAG GTGTCTCTGT GTTTCCACAG AACCCAGGGA
      121 CTCATGATCA TGAAAACAGT GTACAAGGGG CCCAACTGCA TTGAGCACAA CGAGGCCCTC
      181 TTGGAGGAGG CGAAGATGAT GAACAGACTG AGACACAGCC GGGTGGTGAA GCTCCTGGGC
      241 GTCATCATAG AGGAAGGGAA GTACTCCCTG GTGATGGAGT ACATGGAGAA GGGCAACCTG
      301 ATGCACGTGC TGAAAGCCGA GATGAGTACT CCGCTTTCTG TAAAAGGAAG GATAATTTTG
      361 GAAATCATTG AAGGAATGTG CTACTTACAT GGAAAAGGCG TGATACACAA GAACCTGAAG
      421 CCTGAAAATA TCCTTGTTGA TAATGACTTC CACATTAAGA TCGCAGACCT CGGCCTTGCC
      481 TCCTTTAAGA TGTGGAGCAA ACTGAATAAT GAAGAGCACA ATGAGCTGAG GGAAGTGGAC
      541 GGCACCGCTA AGAAGAATGG CGGCACCCTC TACTACATGG CGCCCGAGCA CCTGAATGAC
      601 GTCAACGCAA AGCCCACAGA GAAGTCGGAT GTGTACAGCT TTGCTGTAGT ACTCTGGGCG
      661 ATATTTGCAA ATAAGGAGCC ATATGAAAAT GCTATCTGTG AGCAGCAGTT GATAATGTGC
      721 ATAAAATCTG GGAACAGGCC AGATGTGGAT GACATCACTG AGTACTGCCC AAGAGAAATT
      781 ATCAGTCTCA TGAAGCTCTG CTGGGAAGCG AATCCGGAAG CTCGGCCGAC ATTTCCTGGC
      841 ATTGAAGAAA AATTTAGGCC TTTTTATTTA AGTCAATTAG AAGAAAGTGT AGAAGAGGAC
      901 GTGAAGAGTT TAAAGAAAGA GTATTCAAAC GAAAATGCAG TTGTGAAGAG AATGCAGTCT
      961 CTTCAACTTG ATTGTGTGGC AGTACCTTCA AGCCGGTCAA ATTCAGCCAC AGAACAGCCT
     1021 GGTTCACTGC ACAGTTCCCA GGGACTTGGG ATGGGTCCTG TGGAGGAGTC CTGGTTTGCT
     1081 CCTTCCCTGG AGCACCCACA AGAAGAGAAT GAGCCCAGCC TGCAGAGTAA ACTCCAAGAC
     1141 GAAGCCAACT ACCATCTTTA TGGCAGCCGC ATGGACAGGC AGACGAAACA GCAGCCCAGA
     1201 CAGAATGTGG CTTACAACAG AGAGGAGGAA AGGAGACGCA GGGTCTCCCA TGACCCTTTT
     1261 GCACAGCAAA GACCTTACGA GAATTTTCAG AATACAGAGG GAAAAGGCAC TGCTTATTCC
     1321 AGTGCAGCCA GTCATGGTAA TGCAGTGCAC CAGCCCTCAG GGCTCACCAG CCAACCTCAA
     1381 GTACTGTATC AGAACAATGG ATTATATAGC TCACATGGCT TTGGAACAAG ACCACTGGAT
     1441 CCAGGAACAG CAGGTCCCAG AGTTTGGTAC AGGCCAATTC CAAGTCATAT GCCTAGTCTG
     1501 CATAATATCC CAGTGCCTGA GACCAACTAT CTAGGAAATA CACCCACCAT GCCATTCAGC
     1561 TCCTTGCCAC CAACAGATGA ATCTATAAAA TATACCATAT ACAATAGTAC TGGCATTCAG
     1621 ATTGGAGCCT ACAATTATAT GGAGATTGGT GGGACGAGTT CATCACTACT AGACAGCACA
     1681 AATACGAACT TCAAAGAAGA GCCAGCTGCT AAGTACCAAG CTATCTTTGA TAATACCACT
     1741 AGTCTGACGG ATAAACACCT GGACCCAATC AGGGAAAATC TGGGAAAGCA CTGGAAAAAC
     1801 TGTGCCCGTA AACTGGGCTT CACACAGTCT CAGATTGATG AAATTGACCA TGACTATGAG
     1861 CGAGATGGAC TGAAAGAAAA GGTTTACCAG ATGCTCCAAA AGTGGGTGAT GAGGGAAGGC
     1921 ATAAAGGGAG CCACGGTGGG GAAGCTGGCC CAGGCGCTCC ACCAGTGTTC CAGGATCGAC
     1981 CTTCTGAGCA GCTTGATTTA CGTCAGCCAG AACTAATACC CAGCTTTCTT GTACAAAGTG
     2041 GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA
     2101 AATAAAATCA TTATTTGCCA TCCAGCTGCA GCTCTGGCCC GTGTCTCAAA ATCTCTGATG
     2161 TTACATTGCA CAAGATAAAA ATATATCATC ATGAACAATA AAACTGTCTG CTTACATAAA
     2221 CAGTAATACA AGGGGTGTTA TGAGCCATAT TCAACGGGAA ACGTCGAGGC CGCGATTAAA
     2281 TTCCAACATG GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG TCGGGCAATC
     2341 AGGTGCGACA ATCTATCGCT TGTATGGGAA GCCCGATGCG CCAGAGTTGT TTCTGAAACA
     2401 TGGCAAAGGT AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGACTAA ACTGGCTGAC
     2461 GGAATTTATG CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG ATGCATGGTT
     2521 ACTCACCACT GCGATCCCCG GAAAAACAGC ATTCCAGGTA TTAGAAGAAT ATCCTGATTC
     2581 AGGTGAAAAT ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT CGATTCCTGT
     2641 TTGTAATTGT CCTTTTAACA GCGATCGCGT ATTTCGTCTC GCTCAGGCGC AATCACGAAT
     2701 GAATAACGGT TTGGTTGATG CGAGTGATTT TGATGACGAG CGTAATGGCT GGCCTGTTGA
     2761 ACAAGTCTGG AAAGAAATGC ATAAACTTTT GCCATTCTCA CCGGATTCAG TCGTCACTCA
     2821 TGGTGATTTC TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAGGCC GGGAAGCCGA
     2881 TCTCGGCTTG AACGAATTGT TAGGTGGCGG TACTTGGGTC GATATCAAAG TGCATCACTT
     2941 CTTCCCGTAT GCCCAACTTT GTATAGAGAG CCACTGCGGG ATCGTCACCG TAATCTGCTT
     3001 GCACGTAGAT CACATAAGCA CCAAGCGCGT TGGCCTCATG CTTGAGGAGA TTGATGAGCG
     3061 CGGTGGCAAT GCCCTGCCTC CGGTGCTCGC CGGAGACTGC GAGATCATAG ATATAGATCT
     3121 CACTACGCGG CTGCTCAAAC CTGGGCAGAA CGTAAGCCGC GAGAGCGCCA ACAACCGCTT
     3181 CTTGGTCGAA GGCAGCAAGC GCGATGAATG TCTTACTACG GAGCAAGTTC CCGAGGTAAT
     3241 CGGAGTCCGG CTGATGTTGG GAGTAGGTGG CTACGTCTCC GAACTCACGA CCGAAAAGAT
     3301 CAAGAGCAGC CCGCATGGAT TTGACTTGGT CAGGGCCGAG CCTACATGTG CGAATGATGC
     3361 CCATACTTGA GCCACCTAAC TTTGTTTTAG GGCGACTGCC CTGCTGCGTA ACATCGTTGC
     3421 TGCTGCGTAA CATCGTTGCT GCTCCATAAC ATCAAACATC GACCCACGGC GTAACGCGCT
     3481 TGCTGCTTGG ATGCCCGAGG CATAGACTGT ACAAAAAAAC AGTCATAACA AGCCATGAAA
     3541 ACCGCCACTG CGCCGTTACC ACCGCTGCGT TCGGTCAAGG TTCTGGACCA GTTGCGTGAG
     3601 CGCATACGCT ACTTGCATTA CAGTTTACGA ACCGAACAGG CTTATGTCAA CTGGGTTCGT
     3661 GCCTTCATCC GTTTCCACGG TGTGCGTCAC CCGGCAACCT TGGGCAGCAG CGAAGTCGAG
     3721 GCATTTCTGT CCTGGCTGGC GAACGAGCGC AAGGTTTCGG TCTCCACGCA TCGTCAGGCA
     3781 TTGGCGGCCT TGCTGTTCTT CTACGGCAAG GTGCTGTGCA CGGATCTGCC CTGGCTTCAG
     3841 GAGATCGGAA GACCTCGGCC GTCGCGGCGC TTGCCGGTGG TGCTGACCCC GGATGAAGTG
     3901 GTTCGCATCC TCGGTTTTCT GGAAGGCGAG CATCGTTTGT TCGCCCAGGA CTCTAGCTAT
     3961 AGTTCTAGTG GTTGGCTACT AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC
     4021 CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG
     4081 AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT
     4141 TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA CTGGCAGAGC
     4201 ATTACGCTGA CTTGACGGGA CGGCGCAAGC TCATGACCAA AATCCCTTAA CGTGAGTTTT
     4261 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT
     4321 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT
     4381 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA
     4441 TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG
     4501 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA
     4561 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG
     4621 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA
     4681 GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA
     4741 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA
     4801 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT
     4861 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC
     4921 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT
     4981 CTGTGGATAA CCGTATTACC GCTAGCCAGG AAGAGTTTGT AGAAACGCAA AAAGGCCATC
     5041 CGTCAGGATG GCCTTCTGCT TAGTTTGATG CCTGGCAGTT TATGGCGGGC GTCCTGCCCG
     5101 CCACCCTCCG GGCCGTTGCT TCACAACGTT CAAATCCGCT CCCGGCGGAT TTGTCCTACT
     5161 CAGGAGAGCG TTCACCGACA AACAACAGAT AAAACGAAAG GCCCAGTCTT CCGACTGAGC
     5221 CTTTCGTTTT ATTTGATGCC TGGCAGTTCC CTACTCTCGC GTTAACGCTA GCATGGATCT
     5281 CGGGCCCCAA ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA
     5341 TGAGCAATGC TTTTTTATAA TGCCAAGTTT GTACAAAAAA GCAGGCTTAC TGGAAGTTCT
     5401 GTTCCAGGGG CCC
//
