LOCUS       pENTR3C-GFP-mPARK2-mut      4422 bp    DNA     circular SYN 09-FEB-2012
DEFINITION  LMBP 8689.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8689
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
DBSOURCE    GenBank
            Unknown
            Unclassified
FEATURES             Location/Qualifiers
     Source          <1..>2281
                     /invitrogen="563"
                     /label=Source_1
     Misc._recombina complement(14..113)
                     /invitrogen="2680000"
                     /label=attL2
     CDS             236..1045
                     /invitrogen="1210000"
                     /label=Kan(R)
     Replication_ori 1109..1782
                     /invitrogen="2540000"
                     /label="pUC origin"
     Terminator      complement(1891..1934)
                     /invitrogen="2580000"
                     /label="rrnB T1 transcription terminator"
     Terminator      complement(2066..2093)
                     /invitrogen="2590000"
                     /label="rrnB T2 transcription terminator"
     Misc._recombina 2143..2242
                     /invitrogen="2670000"
                     /label=attL1
     CDS             2282..3021
                     /label="GFP + linker"
     CDS             3022..4419
                     /label=mParkin
ORIGIN
        1 TCGAGATATC TAGACCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA
       61 TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA
      121 GCTGCAGCTC TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAAATAT
      181 ATCATCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG
      241 CCATATTCAA CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA
      301 TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA
      361 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA
      421 TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT
      481 CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGAAA
      541 AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT
      601 GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA
      661 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG
      721 TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA
      781 ACTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT
      841 TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA
      901 CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA
      961 GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA
     1021 TTTGATGCTC GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ATTATTCAGA
     1081 TTGGGCCCCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA
     1141 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT
     1201 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG
     1261 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA
     1321 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG
     1381 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA
     1441 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC
     1501 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA
     1561 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC
     1621 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG
     1681 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC
     1741 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC
     1801 CCCTGATTCT GTGGATAACC GTATTACCGC TAGCATGGAT CTCGGGGACG TCTAACTACT
     1861 AAGCGAGAGT AGGGAACTGC CAGGCATCAA ATAAAACGAA AGGCTCAGTC GGAAGACTGG
     1921 GCCTTTCGTT TTATCTGTTG TTTGTCGGTG AACGCTCTCC TGAGTAGGAC AAATCCGCCG
     1981 GGAGCGGATT TGAACGTTGT GAAGCAACGG CCCGGAGGGT GGCGGGCAGG ACGCCCGCCA
     2041 TAAACTGCCA GGCATCAAAC TAAGCAGAAG GCCATCCTGA CGGATGGCCT TTTTGCGTTT
     2101 CTACAAACTC TTCCTGTTAG TTAGTTACTT AAGCTCGGGC CCCAAATAAT GATTTTATTT
     2161 TGACTGATAG TGACCTGTTC GTTGCAACAA ATTGATAAGC AATGCTTTTT TATAATGCCA
     2221 ACTTTGTACA AAAAAGCAGG CTCTTTAAAG GAACCAATTC AGTCGACTGG ATCCGGTACC
     2281 GAATTCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC
     2341 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT
     2401 GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC
     2461 TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC
     2521 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC
     2581 ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC
     2641 GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC
     2701 CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG
     2761 CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG
     2821 CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC
     2881 GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT
     2941 CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG
     3001 TACAAGTCCG GACTCAGATC CATGATAGTG TTTGTCAGGT TCAACTCCAG CTATGGCTTC
     3061 CCAGTGGAGG TCGATTCTGA CACCAGCATC TTGCAGCTCA AGGAAGTGGT TGCTAATCGA
     3121 CAGGGGGTTC CAGCTGACCA GCTGCGTGTG ATTTTTGCCG GGAAGGAGCT TCCGAATCAC
     3181 CTGACGGTTC AAAACTGTGA CCTGGAACAA CAGAGTATTG TACACATAGT ACAGAGACCA
     3241 CGGAGGAGAA GTCATGAAAC AAATGCATCT GGAGGGGACG AACCCCAGAG CACCTCAGAG
     3301 GGCTCCATAT GGGAGTCCAG GAGCTTGACA CGAGTGGACC TGAGCAGCCA TACCCTGCCG
     3361 GTGGACTCTG TGGGGCTGGC GGTCATTCTG GACACAGACA GTAAGAGGGA TTCAGAAGCA
     3421 GCCAGAGGTC CAGCAGTTAA ACCCACCTAC AACAGCTTTT TCATCTACTG CAAAGGCCCC
     3481 TGCCACAAGG TCCAGCCTGG AAAGCTCCGA GTTCAGTGTG GCACCTGCAA ACAAGCAACC
     3541 CTCACCTTGG CCCAGGGCCC ATCTTGCTGG GACGATGTCT TAATTCCAAA CCGGATGAGT
     3601 GGTGAGTGCC AGTCTCCAGA CTGCCCTGGA ACCAGAGCTG AATTTTTCTT TAAATGTGGA
     3661 GCACACCCAA CCTCAGACAA GGACACGTCG GTAGCTTTGA ACCTGATCAC CAGCAACAGG
     3721 CGCAGCATCC CTTGCATAGC GTGCACAGAT GTCAGGAGCC CTGTCCTGGT CTTCCAGTGT
     3781 AACCACCGTC ACGTGATCTG TTTGGACTGT TTCCACTTGT ATTGTGTCAC AAGACTCAAC
     3841 GATCGGCAGT TTGTCCACGA TGCTCAACTT GGCTACTCCC TGCCGTGTGT AGCTGGCTGT
     3901 CCCAACTCCC TGATTAAAGA GCTCCATCAC TTCAGGATCC TTGGAGAAGA GCAGTACACT
     3961 AGGTACCAGC AGTATGGGGC CGAGGAATGC GTGCTGCAAA TGGGAGGTGT GCTGTGCCCC
     4021 CGTCCTGGCT GTGGAGCTGG ACTGCTACCT GAACAGGGCC AGAGGAAAGT CACCTGCGAA
     4081 GGGGGCAACG GCCTGGGCTG CGGGTTTGTT TTCTGCCGGG ACTGTAAGGA AGCATACCAT
     4141 GAAGGGGATT GCGACTCACT GCTTGAACCC TCAGGAGCCA CTTCTCAGGC CTACAGGGTG
     4201 GACAAAAGAG CCGCTCAGCA AGCTCGCTGG GAGGAGGCCT CCAAGGAAAC CATCAAGAAG
     4261 ACCACCAAGC CTTGTCCTCG CTGCAACGTG CCAATTGAAA AAAACGGAGG ATGTATGCAC
     4321 ATGAAGTGTC CTCAGCCCCA GTGCAAGCTG GAGTGGTGCT GGAACTGTGG CTGTGAGTGG
     4381 AACCGAGCCT GCATGGGAGA TCACTGGTTT GACGTGTAGC AC
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