LOCUS       pENTR3C-strep-hMLKL(1-125)-stop      2700 bp    DNA     circular SYN 09-FEB-2012
DEFINITION  LMBP 8695.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     Source          join(<916..2700,1..>496)
                     /label=Source_1
     Vector          join(916..2700,1..496)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
     Terminator      complement(106..149)
                     /label="rrnB T1 transcription terminator"
     Terminator      complement(281..308)
                     /label="rrnB T2 transcription terminator"
     Misc._recombina 358..457
                     /label=attL1
     CDS             541..912
                     /origin="strep + hMLKL(1-125) fragment + primers (445bp
                     linear)           Fragment: from EcoRI cut at 11^12 to
                     XhoI cut at 430^431, 419bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /label="hMLKL (1 - 125) - stop"
     Misc._recombina complement(929..1028)
                     /label=attL2
     CDS             1151..1960
                     /label=Kan(R)
     Replication_ori 2024..2697
                     /label="pUC origin"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG
       61 GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT
      121 CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
      181 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG
      241 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT
      301 GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA
      361 ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC
      421 TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG
      481 ACTGGATCCG GTACCGAATT CACCATGGCT AGCTGGAGCC ACCCGCAGTT CGAAAAAGCC
      541 GAAAATTTGA AGCATATTAT CACCCTTGGC CAGGTCATCC ACAAACGGTG TGAAGAGATG
      601 AAATACTGCA AGAAACAGTG CCGGCGCCTG GGCCACCGCG TCCTCGGCCT GATCAAGCCT
      661 CTGGAGATGC TCCAGGACCA AGGAAAGAGG AGCGTGCCCT CTGAGAAGTT AACCACAGCC
      721 ATGAACCGCT TCAAGGCTGC CCTGGAGGAG GCTAATGGGG AGATAGAAAA GTTCAGCAAT
      781 AGATCCAATA TCTGCAGGTT TCTAACAGCA AGCCAGGACA AAATACTCTT CAAGGACGTG
      841 AACAGGAAGC TGAGTGATGT CTGGAAGGAG CTCTCGCTGT TACTTCAGGT TGAGCAACGC
      901 ATGCCTGTTT CACACTCGAG ATATCTAGAC CCAGCTTTCT TGTACAAAGT TGGCATTATA
      961 AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC
     1021 ATTATTTGCC ATCCAGCTGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT GTTACATTGC
     1081 ACAAGATAAA AATATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC
     1141 AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT
     1201 GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC
     1261 AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG
     1321 TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT
     1381 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC
     1441 TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA
     1501 TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG
     1561 TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG
     1621 TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG
     1681 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT
     1741 CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG
     1801 AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT
     1861 TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA
     1921 TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG TTAATTGGTT
     1981 GTAACATTAT TCAGATTGGG CCCCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA
     2041 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC
     2101 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT
     2161 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG
     2221 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC
     2281 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT
     2341 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA
     2401 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT
     2461 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG
     2521 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA
     2581 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA
     2641 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT
//
