LOCUS       pENTR3C-strep-hMLKL(FL)-K230M-stop      3738 bp    DNA     circular SYN 09-FEB-2012
DEFINITION  LMBP 8697.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     Source          join(<1954..3738,1..>496)
                     /label=Source_1
     Vector          join(1954..3738,1..496)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
     Terminator      complement(106..149)
                     /label="rrnB T1 transcription terminator"
     Terminator      complement(281..308)
                     /label="rrnB T2 transcription terminator"
     Misc._recombina 358..457
                     /label=attL1
     Misc._signal    513..536
                     /origin="strep + hMLKL( + primer) (1.486bp linear)
                     Fragment: from EcoRI cut at 11^12 to XhoI cut at
                     1471^1472, 1.460bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /label="strep tag"
     CDS             541..1950
                     /label="hMLKL(FL) - K230M - stop"
     Misc._recombina complement(1967..2066)
                     /label=attL2
     CDS             2189..2998
                     /label=Kan(R)
     Replication_ori 3062..3735
                     /label="pUC origin"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG
       61 GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT
      121 CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
      181 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG
      241 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT
      301 GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA
      361 ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC
      421 TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG
      481 ACTGGATCCG GTACCGAATT CACCATGGCT AGCTGGAGCC ACCCGCAGTT CGAAAAAGCC
      541 GAAAATTTGA AGCATATTAT CACCCTTGGC CAGGTCATCC ACAAACGGTG TGAAGAGATG
      601 AAATACTGCA AGAAACAGTG CCGGCGCCTG GGCCACCGCG TCCTCGGCCT GATCAAGCCT
      661 CTGGAGATGC TCCAGGACCA AGGAAAGAGG AGCGTGCCCT CTGAGAAGTT AACCACAGCC
      721 ATGAACCGCT TCAAGGCTGC CCTGGAGGAG GCTAATGGGG AGATAGAAAA GTTCAGCAAT
      781 AGATCCAATA TCTGCAGGTT TCTAACAGCA AGCCAGGACA AAATACTCTT CAAGGACGTG
      841 AACAGGAAGC TGAGTGATGT CTGGAAGGAG CTCTCGCTGT TACTTCAGGT TGAGCAACGC
      901 ATGCCTGTTT CACCCATAAG CCAAGGAGCG TCCTGGGCAC AGGAAGATCA GCAGGATGCA
      961 GACGAAGACA GGCGAGCTTT CCAGATGCTA AGAAGAGATA ATGAAAAAAT AGAAGCTTCA
     1021 CTGAGACGAT TAGAAATCAA CATGAAAGAA ATCAAGGAAA CTTTGAGGCA GTATTTACCA
     1081 CCAAAATGCA TGCAGGAGAT CCCGCAAGAG CAAATCAAGG AGATCAAGAA GGAGCAGCTT
     1141 TCAGGATCCC CGTGGATTCT GCTAAGGGAA AATGAAGTCA GCACACTTTA TAAAGGAGAA
     1201 TACCACAGAG CTCCAGTGGC CATAATGGTA TTCAAAAAAC TCCAGGCTGG CAGCATTGCA
     1261 ATAGTGAGGC AGACTTTCAA TAAGGAGATC AAAACCATGA AGAAATTCGA ATCTCCCAAC
     1321 ATCCTGCGTA TATTTGGGAT TTGCATTGAT GAAACAGTGA CTCCGCCTCA ATTCTCCATT
     1381 GTCATGGAGT ACTGTGAACT CGGGACCCTG AGGGAGCTGT TGGATAGGGA AAAAGACCTC
     1441 ACACTTGGCA AGCGCATGGT CCTAGTCCTG GGGGCAGCCC GAGGCCTATA CCGGCTACAC
     1501 CATTCAGAAG CACCTGAACT CCACGGAAAA ATCAGAAGCT CAAACTTCCT GGTAACTCAA
     1561 GGCTACCAAG TGAAGCTTGC AGGATTTGAG TTGAGGAAAA CACAGACTTC CATGAGTTTG
     1621 GGAACTACGA GAGAAAAGAC AGACAGAGTC AAATCTACAG CATATCTCTC ACCTCAGGAA
     1681 CTGGAAGATG TATTTTATCA ATATGATGTA AAGTCTGAAA TATACAGCTT TGGAATCGTC
     1741 CTCTGGGAAA TCGCCACTGG AGATATCCCG TTTCAAGGCT GTAATTCTGA GAAGATCCGC
     1801 AAGCTGGTGG CTGTGAAGCG GCAGCAGGAG CCACTGGGTG AAGACTGCCC TTCAGAGCTG
     1861 CGGGAGATCA TTGATGAGTG CCGGGCCCAT GATCCCTCTG TGCGGCCCTC TGTGGATGAA
     1921 ATCTTAAAGA AACTCTCCAC CTTTTCTAAG CACTCGAGAT ATCTAGACCC AGCTTTCTTG
     1981 TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT
     2041 ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGCAG CTCTGGCCCG TGTCTCAAAA
     2101 TCTCTGATGT TACATTGCAC AAGATAAAAA TATATCATCA TGAACAATAA AACTGTCTGC
     2161 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC
     2221 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT
     2281 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT
     2341 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA
     2401 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA
     2461 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA
     2521 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC
     2581 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA
     2641 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG
     2701 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT
     2761 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG
     2821 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG
     2881 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT
     2941 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC
     3001 AGAATTGGTT AATTGGTTGT AACATTATTC AGATTGGGCC CCGTTCCACT GAGCGTCAGA
     3061 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG
     3121 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC
     3181 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT
     3241 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC
     3301 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT
     3361 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG
     3421 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT
     3481 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG
     3541 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG
     3601 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG
     3661 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG
     3721 GCCTTTTGCT CACATGTT
//
