LOCUS       pLenti6(puro)-FLAG-dest-strep-hMLKL(1-125)-stop      7627 bp    DNA     circular SYN 09-FEB-2012
DEFINITION  LMBP 8711.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     Misc._recombina join(7621..7627,1..14)
                     /label=attB1
     Source          join(<476..>498,<1..>56)
                     /label=Source_1
     Vector          join(476..>498,<1..56)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
     Misc._signal    65..100
                     /label="Strep Tag"
     CDS             101..472
                     /origin="strep + hMLKL(1-125) fragment + primers (445bp
                     linear)           Fragment: from EcoRI cut at 11^12 to
                     XhoI cut at 430^431, 419bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /label="hMLKL (1 - 125) - stop"
     Misc._recombina complement(492..512)
                     /label=attB2
     Misc._feature   556..580
                     /label="FLAG tag"
     CDS             593..601
                     /label="3 stops"
     Promoter        638..947
                     /label="SV40 early promoter"
     CDS             1007..1609
                     /label=Puro(R)
     LTR             1700..1753
                     /label=deltaU3
     LTR             1701..1934
                     /label="deltaU3/3' LTR"
     LTR             1754..1934
                     /label="HIV-1 3' LTR"
     PolyA_signal    2006..2136
                     /label="SV40 pA"
     Replication_ori 2509..2964
                     /label="f1 origin"
     Promoter        2996..3094
                     /label="bla promoter"
     CDS             3095..3955
                     /label=Amp(R)
     Replication_ori 4100..4773
                     /label="pUC origin"
     Promoter        5181..5409
                     /label="RSV promoter"
     Promoter        5181..5590
                     /label="RSV/5' LTR hybrid promoter"
     Source          5180^5181
                     /label=Source_1
     LTR             5410..5590
                     /label="HIV-1 5' LTR"
     Misc._feature   5700
                     /label="splice donor"
     Misc._binding   5701..5745
                     /label="HIV-1 psi packaging signal"
     Protein_binding 6255..6488
                     /label="HIV-1 Rev response element"
     Misc._feature   6836
                     /label="splice acceptor"
     Misc._feature   6864
                     /label="splice acceptor"
     Promoter        6989..7571
                     /label="CMV promoter"
     TATA_box        7489..7492
                     /label="TATA box"
ORIGIN
        1 GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG ACTGGATCCG GTACCGAATT
       61 CACCATGGCT AGCTGGAGCC ACCCGCAGTT CGAAAAAGCC GAAAATTTGA AGCATATTAT
      121 CACCCTTGGC CAGGTCATCC ACAAACGGTG TGAAGAGATG AAATACTGCA AGAAACAGTG
      181 CCGGCGCCTG GGCCACCGCG TCCTCGGCCT GATCAAGCCT CTGGAGATGC TCCAGGACCA
      241 AGGAAAGAGG AGCGTGCCCT CTGAGAAGTT AACCACAGCC ATGAACCGCT TCAAGGCTGC
      301 CCTGGAGGAG GCTAATGGGG AGATAGAAAA GTTCAGCAAT AGATCCAATA TCTGCAGGTT
      361 TCTAACAGCA AGCCAGGACA AAATACTCTT CAAGGACGTG AACAGGAAGC TGAGTGATGT
      421 CTGGAAGGAG CTCTCGCTGT TACTTCAGGT TGAGCAACGC ATGCCTGTTT CACACTCGAG
      481 ATATCTAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC CAGCACAGTG GCGGCCGCTC
      541 GAGTCTAGAG GGCCCGGACT ACAAGGATGA CGATGACAAA ACGCGTACCG GTTAGTAATG
      601 AGTTTGGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC
      661 CCAGGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA
      721 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC
      781 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC
      841 TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC
      901 TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT
      961 CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGACCATGG CCACCGAGTA
     1021 CAAGCCCACG GTGCGCCTCG CCACCCGCGA CGACGTCCCC CGGGCCGTAC GCACCCTCGC
     1081 CGCCGCGTTC GCCGACTACC CCGCCACGCG CCACACCGTC GACCCGGACC GCCACATCGA
     1141 GCGGGTCACC GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT
     1201 GTGGGTCGCG GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC
     1261 GGGGGCGGTG TTCGCCGAGA TCGGCTCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC
     1321 CGCGCAGCAA CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT
     1381 CCTGGCCACC GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT
     1441 GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC TGGGGTGCCC GCCTTCCTGG AGACCTCCGC
     1501 GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT
     1561 GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAG CTAAGCACAA
     1621 TTCGAGCTCG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT
     1681 TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATCTGCT
     1741 TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA
     1801 ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG
     1861 TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG
     1921 GAAAATCTCT AGCAGTAGTA GTTCATGTCA TCTTATTATT CAGTATTTAT AACTTGCAAA
     1981 GAAATGAATA TCAGAGAGTG AGAGGAACTT GTTTATTGCA GCTTATAATG GTTACAAATA
     2041 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG
     2101 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGCTC TAGCTATCCC GCCCCTAACT
     2161 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA
     2221 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG
     2281 TGAGGAGGCT TTTTTGGAGG CCTAGGGACG TACCCAATTC GCCCTATAGT GAGTCGTATT
     2341 ACGCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC
     2401 AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC
     2461 GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAC GCGCCCTGTA
     2521 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA
     2581 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT
     2641 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC
     2701 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT
     2761 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC
     2821 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC
     2881 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA
     2941 ACAAAATATT AACGCTTACA ATTTAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC
     3001 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG
     3061 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC
     3121 CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT
     3181 GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT
     3241 CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC
     3301 TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT
     3361 CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA
     3421 GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA
     3481 TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT
     3541 TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA
     3601 AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG
     3661 CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT
     3721 GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT
     3781 TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC
     3841 AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA
     3901 TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC
     3961 AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG
     4021 GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC
     4081 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT
     4141 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT
     4201 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT
     4261 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC
     4321 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA
     4381 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG
     4441 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG
     4501 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG
     4561 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA
     4621 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT
     4681 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG
     4741 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC
     4801 TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC
     4861 CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT
     4921 CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC
     4981 GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT
     5041 ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC
     5101 AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA
     5161 AAAGCTGGAG CTGCAAGCTT AATGTAGTCT TATGCAATAC TCTTGTAGTC TTGCAACATG
     5221 GTAACGATGA GTTAGCAACA TGCCTTACAA GGAGAGAAAA AGCACCGTGC ATGCCGATTG
     5281 GTGGAAGTAA GGTGGTACGA TCGTGCCTTA TTAGGAAGGC AACAGACGGG TCTGACATGG
     5341 ATTGGACGAA CCACTGAATT GCCGCATTGC AGAGATATTG TATTTAAGTG CCTAGCTCGA
     5401 TACATAAACG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA
     5461 GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC
     5521 CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA
     5581 ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA AGGGAAACCA GAGGAGCTCT
     5641 CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG AGGCGAGGGG CGGCGACTGG
     5701 TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA GAGAGATGGG TGCGAGAGCG
     5761 TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA AATTCGGTTA AGGCCAGGGG
     5821 GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA GAACGATTCG
     5881 CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG GGACAGCTAC
     5941 AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA GTAGCAACCC
     6001 TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA GGAAGCTTTA GACAAGATAG
     6061 AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGC GGCCGCTGAT CTTCAGACCT
     6121 GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA AGTAGTAAAA
     6181 ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGTGCA GAGAGAAAAA
     6241 AGAGCAGTGG GAATAGGAGC TTTGTTCCTT GGGTTCTTGG GAGCAGCAGG AAGCACTATG
     6301 GGCGCAGCGT CAATGACGCT GACGGTACAG GCCAGACAAT TATTGTCTGG TATAGTGCAG
     6361 CAGCAGAACA ATTTGCTGAG GGCTATTGAG GCGCAACAGC ATCTGTTGCA ACTCACAGTC
     6421 TGGGGCATCA AGCAGCTCCA GGCAAGAATC CTGGCTGTGG AAAGATACCT AAAGGATCAA
     6481 CAGCTCCTGG GGATTTGGGG TTGCTCTGGA AAACTCATTT GCACCACTGC TGTGCCTTGG
     6541 AATGCTAGTT GGAGTAATAA ATCTCTGGAA CAGATTTGGA ATCACACGAC CTGGATGGAG
     6601 TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA
     6661 AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG
     6721 AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA
     6781 GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG
     6841 GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC
     6901 GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC
     6961 GGATCTCGAC GGTATCGATA AGCTTGGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC
     7021 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC
     7081 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT
     7141 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT
     7201 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT
     7261 TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC
     7321 ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC
     7381 GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC
     7441 TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA
     7501 GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT
     7561 AGAAGACACC GACTCTAGAG GATCCACTAG TCCAGTGTGG TGGAATTCTG CAGATATCAA
     7621 CAAGTTT
//
