LOCUS pLenti6(puro)-FLAG-dest-strep-mRIP1-stop 9220 bp DNA circular SYN 18-MAR-2015 DEFINITION LMBP 8806. ACCESSION pIB0097 - pLenti6/FLAG-DEST-puro SOURCE ORGANISM COMMENT Accession number: 8806 Last modified: 18-MAR-2015 COMMENT VNTDATE|728671520| COMMENT VNTNAME|pLenti6(puro)-FLAG-dest-strep-mRIP1-stop| COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTREPLTYPE|Phage COMMENT VNTREPLTYPE|Virus COMMENT VNTEXTCHREPL|Bacteria COMMENT VNTEXTCHREPL|Animal/Other Eukaryotic COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pLenti6(puro)-FLAG-dest-strep-mRIP1-stop" 0 9220 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 COMMENT 0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) COMMENT (CWordArray) (CStringList "AgeI" "ApaI" "NdeI" "NheI" "XmnI") COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 COMMENT "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 1 955 951 1 9220 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 90 0 0 "") COMMENT #1=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 57 0 7379 0 0 "") COMMENT #2=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 6672 0 0 "") COMMENT #3=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 7198 0 0 "") COMMENT #4=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 5 114 0 2785 0 5303 0 COMMENT 7160 0 8184 0 0 "")) COMMENT (CObList COMMENT #5=(CFSignal (CObList) "FLAG tag" 21 0 0 58 82 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "58..82") COMMENT #6=(CFSignal (CObList) "SV40 early promoter" 29 0 0 118 400 0 COMMENT "140..449") COMMENT #7=(CFSignal (CObList) "puromycin resistance" 4 0 0 509 1111 0 COMMENT "509..1111") COMMENT #8=(CFSignal (CObList) "ampicillin resistance" 4 0 0 2597 3457 0 COMMENT "2597..3457") COMMENT #9=(CFSignal (CObList) "HIV-1 psi packaging signal" 21 0 0 5203 5247 0 COMMENT "5203..5247") COMMENT #10=(CFSignal (CObList) "HIV-1 Rev response element" 21 0 0 5757 5990 0 COMMENT "5757..5990") COMMENT #11=(CFSignal (CObList) "strep" 4 0 0 7194 7229 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "7194..7229") COMMENT #12=(CFSignal (CObList) "mRIPK1-start-stop" 4 0 0 7230 9194 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "7230..9194") COMMENT #13=(CFSignal (CObList) "T3 promoter" 30 0 0 4639 4655 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #14=(CFSignal (CObList) "pMB1 ori" 33 0 0 3998 4400 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #15=(CFSignal (CObList) "lac promoter" 30 0 0 4492 4575 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #16=(CFSignal (CObList) "RSV/HIV-1 5' LTR" 29 0 0 4764 5092 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #17=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 6489 7005 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #18=(CFSignal (CObList) "SV40 ori" 33 0 0 357 432 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #19=(CFSignal (CObList) "HIV-1 cPPT" 21 0 0 1186 1201 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #20=(CFSignal (CObList) "RSV/HIV-1 dU3/3' LTR" 43 0 0 1202 1436 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #21=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1508 1582 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #22=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1508 1582 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #23=(CFSignal (CObList) "SV40 ori" 33 0 0 1718 1793 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #24=(CFSignal (CObList) "T7 promoter" 30 0 1 1827 1843 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #25=(CFSignal (CObList) "f1 ori" 33 0 0 2011 2466 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #26=(CFSignal (CObList) "attB1" 0 0 0 7122 7146 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #27=(CFSignal (CObList) "attB2" 0 0 1 9211 15 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) COMMENT (CObList COMMENT #28=(CFSignal (CObList) "Collection N\260.: 8806" 2 0 0 0 0 0 COMMENT (CStringList) (CStringList) 1 1 0 0 "") COMMENT #29=(CFSignal (CObList) "strep-mRIP1-stop" 2 0 0 0 0 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "") COMMENT #30=(CFSignal (CObList) "Last modified: 18-MAR-2015" 2 0 0 0 0 0 COMMENT (CStringList) (CStringList) 1 1 0 0 "")) (CObList) COMMENT (CTextView 0 COMMENT #31=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #32=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) COMMENT "pLenti6(puro)-FLAG-dest-strep-mRIP1-stop" 2) 5 "" 0 4) COMMENT #33=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA Plasmid Phage Virus 'pLenti6(puro)-FLAG-dest-strep-mRIP1-stop'" COMMENT 1) COMMENT #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Collection N.\260: 8806." 1) COMMENT #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Currently local object. 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(CObList) 1 (CObList)) (CObList) 28184008 COMMENT (CStringList) 34078830 14746016 COMMENT (CObList COMMENT #345=(CSampFrag "pLenti6(puro)-FLAG-dest-strep-mRIP1-stop" 2671 114 COMMENT "XmnI" 2785 "XmnI" (LOGPEN 0 0 0) 1.37913e-316 2.18422e-300) COMMENT #346=(CSampFrag "pLenti6(puro)-FLAG-dest-strep-mRIP1-stop" 2518 2785 COMMENT "XmnI" 5303 "XmnI" (LOGPEN 0 0 0) 9.1033e-283 4.69778e-294) COMMENT #347=(CSampFrag "pLenti6(puro)-FLAG-dest-strep-mRIP1-stop" 1857 5303 COMMENT "XmnI" 7160 "XmnI" (LOGPEN 0 0 0) 9.10449e-283 9.10429e-283) COMMENT #348=(CSampFrag "pLenti6(puro)-FLAG-dest-strep-mRIP1-stop" 1150 8184 COMMENT "XmnI" 114 "XmnI" (LOGPEN 0 0 0) 9.10685e-283 4.69761e-294) COMMENT #349=(CSampFrag "pLenti6(puro)-FLAG-dest-strep-mRIP1-stop" 1024 7160 COMMENT "XmnI" 8184 "XmnI" (LOGPEN 0 0 0) 9.10567e-283 4.69748e-294)))) FEATURES Location/Qualifiers Misc._recombina complement(join(9211..9220,1..>7)) /vntifkey="21" /label=attL2 /note="Unknown feature type:Misc._recombina" Misc._recombina complement(join(9214..9220,1..14)) /vntifkey="21" /label=attB2 /note="Unknown feature type:Misc._recombina" Misc._feature 58..82 /vntifkey="21" /label=FLAG\tag /note="Unknown feature type:Misc._feature" promoter 118..400 /vntifkey="29" /label=SV40\early\promoter CDS 509..1111 /vntifkey="4" /label=puromycin\resistance CDS 2597..3457 /vntifkey="4" /label=ampicillin\resistance Source 4682^4683 /vntifkey="21" /label=Source_1 /note="Unknown feature type:Source Oriinal location: 4175..4174" Misc._binding 5203..5247 /vntifkey="21" /label=HIV-1\psi\packaging\signal /note="Unknown feature type:Misc._binding Oriinal location: 4695..4739" Protein_binding 5757..5990 /vntifkey="21" /label=HIV-1\Rev\response\element /note="Unknown feature type:Protein_binding Oriinal location: 5249..5482" Source join(<9198..>9220,<7130..>7185) /vntifkey="21" /label=Source_1 /note="Unknown feature type:Source" Vector join(9198..>9220,<7130..7185) /origin="pENTR3C (2.723bp circular)Target vector: fromXhoI cut at 938^939 to EcoRI cut at 496^497,2.281bpOrientation: originalPlus strand 5': nochangePlus strand 3': no changeMinus strand 5': nochangeMinus strand 3': no change" /vntifkey="21" /label=Misc_Feature_1 /note="Unknown feature type:Vector" CDS 7194..7229 /vntifkey="4" /label=strep CDS 7230..9194 /vntifkey="4" /label=mRIPK1-start-stop promoter 4639..4655 /vntifkey="30" /label=T3\promoter rep_origin 3998..4400 /vntifkey="33" /label=pMB1\ori promoter 4492..4575 /vntifkey="30" /label=lac\promoter promoter 4764..5092 /vntifkey="29" /label=RSV/HIV-1\5'\LTR promoter 6489..7005 /vntifkey="29" /label=hCMV-IE\promoter\and\enhancer rep_origin 357..432 /vntifkey="33" /label=SV40\ori misc_feature 1186..1201 /vntifkey="21" /label=HIV-1\cPPT terminator 1202..1436 /vntifkey="43" /label=RSV/HIV-1\dU3/3'\LTR terminator 1508..1582 /vntifkey="43" /label=SV40\polyA\early terminator complement(1508..1582) /vntifkey="43" /label=SV40\polyA\late rep_origin 1718..1793 /vntifkey="33" /label=SV40\ori promoter complement(1827..1843) /vntifkey="30" /label=T7\promoter rep_origin 2011..2466 /vntifkey="33" /label=f1\ori attenuator 7122..7146 /vntifkey="0" /label=attB1 attenuator complement(9211..15) /vntifkey="0" /label=attB2 BASE COUNT 2493 a 2223 c 2365 g 2139 t ORIGIN 1 cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgga 61 ctacaaggat gacgatgaca aaacgcgtac cggttagtaa tgagtttgga attaattctg 121 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat 181 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 301 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 361 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 421 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 481 cattttcgga tctgatcagc acgtgaccat ggccaccgag tacaagccca cggtgcgcct 541 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta 601 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca 661 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 721 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 781 gatcggctcg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 841 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 901 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 961 ggccgagcgc gctggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 1021 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 1081 ctggtgcatg acccgcaagc ccggtgcctg agctaagcac aattcgagct cggtaccttt 1141 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 1201 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc 1261 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 1321 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 1381 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 1441 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 1501 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 1561 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 1621 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta 1681 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 1741 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 1801 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg 1861 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 1921 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 1981 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg 2041 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 2101 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 2161 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 2221 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 2281 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 2341 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 2401 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta 2461 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 2521 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 2581 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 2641 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 2701 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 2761 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 2821 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 2881 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 2941 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 3001 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 3061 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 3121 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 3181 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 3241 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 3301 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 3361 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 3421 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 3481 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 3541 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 3601 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 3661 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 3721 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 3781 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 3841 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 3901 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 3961 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 4021 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 4081 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 4141 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 4201 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 4261 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 4321 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 4381 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 4441 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 4501 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 4561 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca 4621 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc 4681 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa 4741 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac 4801 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa 4861 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc 4921 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 4981 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 5041 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc 5101 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc 5161 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt 5221 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga 5281 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat 5341 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt 5401 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag 5461 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa 5521 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa 5581 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg 5641 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 5701 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 5761 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 5821 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 5881 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 5941 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 6001 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 6061 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 6121 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 6181 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 6241 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 6301 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 6361 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 6421 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 6481 taagcttggg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc 6541 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg 6601 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat 6661 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc 6721 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta 6781 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag 6841 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt 6901 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa 6961 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt 7021 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgactctag 7081 aggatccact agtccagtgt ggtggaattc tgcagatatc aacaagtttg tacaaaaaag 7141 caggctcttt aaaggaacca attcagtcga ctggatccgg taccgaattc accatggcta 7201 gctggagcca cccgcagttc gaaaaagccc aaccagacat gtccttggac aatattaaga 7261 tggcatccag tgacctgctg gagaagacag acctagacag cggaggcttc gggaaggtgt 7321 ccttgtgtta ccacagaagc catggatttg tcatcctgaa aaaagtatac acagggccca 7381 accgcgctga gtacaatgag gttctcttgg aagaggggaa gatgatgcac agactgagac 7441 acagtcgagt ggtgaagcta ctgggcatca tcatagaaga agggaactat tcgctggtga 7501 tggagtacat ggagaagggc aacctgatgc acgtgctaaa gacccagata gatgtcccac 7561 tttcattgaa aggaaggata atcgtggagg ccatagaagg catgtgctac ttacatgaca 7621 aaggtgtgat acacaaggac ctgaagcctg agaatatcct cgttgatcgt gactttcaca 7681 ttaagatagc cgatcttggt gtggcttcct ttaagacatg gagcaaactg actaaggaga 7741 aagacaacaa gcagaaagaa gtgagcagca ccactaagaa gaacaatggt ggtacccttt 7801 actacatggc acccgaacac ctgaatgaca tcaatgcaaa gcccacggag aagtcggacg 7861 tgtacagctt tggcattgtc ctttgggcaa tatttgcaaa aaaggagccc tatgagaatg 7921 tcatctgtac tgagcagttc gtgatctgca taaaatctgg gaacaggcca aatgtagagg 7981 aaatccttga gtactgtcca agggagatca tcagcctcat ggagcggtgc tggcaggcga 8041 tcccagaaga caggccaaca tttcttggca ttgaagaaga atttaggcct ttttacttaa 8101 gtcattttga agaatatgta gaagaggatg tggcaagttt aaagaaagag tatccagatc 8161 aaagcccagt gctgcagaga atgttttcac tgcagcatga ctgtgtaccc ttacctccga 8221 gcaggtcaaa ttcagaacaa cctggatcgc tgcacagttc ccaggggctc cagatgggtc 8281 ctgtggagga gtcctggttt tcttcctccc cagagtaccc acaggacgag aatgatcgca 8341 gtgtgcaggc taagctgcaa gaggaagcca gctatcatgc ttttggaata tttgcagaga 8401 aacagacaaa accgcagcca aggcagaatg aggcttacaa cagagaggag gaaaggaaac 8461 gaagggtctc tcatgacccc tttgcacagc agagagctcg tgagaatatt aagagtgcag 8521 gagcaagagg tcattctgat cccagcacaa cgagtcgtgg aattgcagtg caacagctgt 8581 catggccagc cacccaaaca gtttggaaca atggattgta taatcagcat ggatttggaa 8641 ctacaggtac aggagtttgg tatccgccaa atctaagcca aatgtatagt acttataaaa 8701 ctccagtgcc agagaccaac ataccgggaa gcacacccac catgccatac ttctctgggc 8761 cagtagcaga tgacctcata aaatatacta tattcaatag ttctggtatt cagattggaa 8821 accacaatta tatggatgtt ggactgaatt cacaaccacc aaacaatact tgcaaagaag 8881 agtcgacttc cagacaccaa gccatctttg ataacaccac tagtctgact gatgaacacc 8941 tgaaccctat cagggaaaac ctgggaaggc agtggaaaaa ctgtgcccgc aagctgggct 9001 tcactgagtc tcagatcgat gaaatcgacc atgactatga aagagatgga ctgaaagaga 9061 aagtttacca aatgcttcag aagtggctga tgcgggaagg caccaaaggg gccacagtgg 9121 gaaagttggc ccaggcactt caccaatgtt gcaggataga cctgctgaac cacttgattc 9181 gtgccagcca gagccactcg agatatctag acccagcttt //