LOCUS       pGEM-mCLDex4-pro        3510 bp    DNA     circular     19-JUL-2016
DEFINITION  LMBP 8826.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|-11332205|
COMMENT     VNTNAME|pGEM-mCLDex4-probeL4|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        (CObList) -1 "") 
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COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 2628 2627 1 3510 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT            (CStringList) 1 1 1 1 "") 
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COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #2=(CFSignal (CObList) "pMB1 ori" 33 0 1 889 1291 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #3=(CFSignal (CObList) "ampicillin resistance" 4 0 1 1832 2692 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #4=(CFSignal (CObList) "f1 ori" 33 0 1 2875 3330 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #5=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 1 676 713 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) "lac promoter" 30 0 1 714 797 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
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COMMENT                         "Created: 07/19/16 01:20" 1) 
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COMMENT                         "Last Modified: 07/19/16 01:20" 1) 
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COMMENT                         "length: 3510 bp" 1) 
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COMMENT                                   "@N" 1 11.5735 12.1963 0 -10 6.86718 1.16519 
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COMMENT                      #113=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -538 100) 
COMMENT                            (CObjectList 
COMMENT                             #114=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #0# 0) 1 2 0 0 #65# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 1.16464 6.2263 0.0303829) 
COMMENT                                   0.4 0.082322 1.08464 1.8 1.8 0) 
COMMENT                             #115=(CLabel 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "T7" "@N" 1 
COMMENT                                   1.54281 13.9386 0 -10 1.09082 1.16519 #114#) 
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COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #1# 0) 1 2 0 0 #65# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 0.835356 5.13651 5.22377) 
COMMENT                                   0.4 0.082322 0.915356 1.8 1.8 1) 
COMMENT                             #117=(CLabel 
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COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SP6" "@N" 1 
COMMENT                                   13.5186 5.22699 0 -10 1.66101 1.16519 #116#) 
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COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #6# 0) 1 2 0 0 #88# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    4.84876 4.99889 1 0.082322) 0.8 1.8 1) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "lac promoter" 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' UTR of lacZ, incl. RBS" "@N" 1 18.3556 
COMMENT                                   3.48466 0 -10 9.76776 1.16519 #121#)) 
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COMMENT                     (CObjectList) (CObjectList)) 
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COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #130=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -726 100) 
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FEATURES             Location/Qualifiers
     promoter        3494..3494
                     /vntifkey="30"
                     /label=T7
     promoter        complement(637..653)
                     /vntifkey="30"
                     /label=SP6
     rep_origin      complement(889..1291)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(1832..2692)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(2875..3330)
                     /vntifkey="33"
                     /label=f1\ori
     RBS             complement(676..713)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(714..797)
                     /vntifkey="30"
                     /label=lac\promoter
     CDS             61..557
                     /vntifkey="4"
                     /label=mCYLD\probe\L4
BASE COUNT      957 a       831 c       856 g       866 t 
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat 
       61 aagacggaga cagagctcaa caagcagaaa tgtattactt taggagtgtc acaaaaagat 
      121 gtcatcacca cttcacaaac ctggatgtgg tatcatatca gatgtgttaa ttaatgcttt 
      181 gggccatcag aaacagcagg gcaaagaggg acatttttat agcaggctga agcaaaggta 
      241 ttgagacacc aacagtaaga aacagttcac tgggaagaat tctttccaaa tctcaccatc 
      301 tattcagctg ctgaaaagga atctttccta cgtgctggaa atgttaattc tgtcccacaa 
      361 atagaggtaa ttgagcaaga aaataaataa ataaactaag gaactaaaga gagactcttc 
      421 atgcttttta cccctcaaga aaacagtgtt aatgatacaa aagatataga tgaaggctta 
      481 ttcaaattaa attaattttc atctaaacaa ctttaccttt taaaagtcta tttgcaagct 
      541 ttattttgtt ggcaaatcac tagtgaattc gcggccgcct gcaggtcgac catatgggag 
      601 agctcccaac gcgttggatg catagcttga gtattctata gtgtcaccta aatagcttgg 
      661 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 
      721 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 
      781 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 
      841 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 
      901 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 
      961 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 
     1021 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 
     1081 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 
     1141 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 
     1201 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 
     1261 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 
     1321 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 
     1381 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 
     1441 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 
     1501 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 
     1561 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 
     1621 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 
     1681 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 
     1741 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 
     1801 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 
     1861 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 
     1921 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 
     1981 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 
     2041 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 
     2101 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 
     2161 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 
     2221 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 
     2281 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 
     2341 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 
     2401 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 
     2461 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 
     2521 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 
     2581 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 
     2641 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 
     2701 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 
     2761 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 
     2821 ctgatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg 
     2881 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 
     2941 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 
     3001 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 
     3061 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 
     3121 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 
     3181 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 
     3241 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 
     3301 ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg 
     3361 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 
     3421 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 
     3481 ggccagtgaa ttgtaatacg actcactata 
//
