LOCUS       pENTR3C-strep-hMLKL(1-180)-stop      2865 bp    DNA     circular SYN 09-FEB-2012
DEFINITION  LMBP 8828.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     Source          join(<1081..2865,1..>496)
                     /label=Source_1
     Vector          join(1081..2865,1..496)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
     Terminator      complement(106..149)
                     /label="rrnB T1 transcription terminator"
     Terminator      complement(281..308)
                     /label="rrnB T2 transcription terminator"
     Misc._recombina 358..457
                     /label=attL1
     Misc._signal    513..536
                     /label="strep tag"
     CDS             541..1077
                     /origin="strep + hMLKL(1-180) fragment + primers (610bp
                     linear)           Fragment: from EcoRI cut at 11^12 to
                     XhoI cut at 595^596, 584bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /label="hMLKL (1-180) - stop"
     Misc._recombina complement(1094..1193)
                     /label=attL2
     CDS             1316..2125
                     /label=Kan(R)
     Replication_ori 2189..2862
                     /label="pUC origin"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG
       61 GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT
      121 CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
      181 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG
      241 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT
      301 GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA
      361 ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC
      421 TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG
      481 ACTGGATCCG GTACCGAATT CACCATGGCT AGCTGGAGCC ACCCGCAGTT CGAAAAAGCC
      541 GAAAATTTGA AGCATATTAT CACCCTTGGC CAGGTCATCC ACAAACGGTG TGAAGAGATG
      601 AAATACTGCA AGAAACAGTG CCGGCGCCTG GGCCACCGCG TCCTCGGCCT GATCAAGCCT
      661 CTGGAGATGC TCCAGGACCA AGGAAAGAGG AGCGTGCCCT CTGAGAAGTT AACCACAGCC
      721 ATGAACCGCT TCAAGGCTGC CCTGGAGGAG GCTAATGGGG AGATAGAAAA GTTCAGCAAT
      781 AGATCCAATA TCTGCAGGTT TCTAACAGCA AGCCAGGACA AAATACTCTT CAAGGACGTG
      841 AACAGGAAGC TGAGTGATGT CTGGAAGGAG CTCTCGCTGT TACTTCAGGT TGAGCAACGC
      901 ATGCCTGTTT CACCCATAAG CCAAGGAGCG TCCTGGGCAC AGGAAGATCA GCAGGATGCA
      961 GACGAAGACA GGCGAGCTTT CCAGATGCTA AGAAGAGATA ATGAAAAAAT AGAAGCTTCA
     1021 CTGAGACGAT TAGAAATCAA CATGAAAGAA ATCAAGGAAA CTTTGAGGCA GTATTTACAC
     1081 TCGAGATATC TAGACCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA
     1141 TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA
     1201 GCTGCAGCTC TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAAATAT
     1261 ATCATCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG
     1321 CCATATTCAA CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA
     1381 TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA
     1441 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA
     1501 TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT
     1561 CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGAAA
     1621 AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT
     1681 GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA
     1741 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG
     1801 TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA
     1861 ACTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT
     1921 TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA
     1981 CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA
     2041 GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA
     2101 TTTGATGCTC GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ATTATTCAGA
     2161 TTGGGCCCCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA
     2221 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT
     2281 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG
     2341 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA
     2401 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG
     2461 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA
     2521 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC
     2581 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA
     2641 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC
     2701 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG
     2761 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC
     2821 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT
//
