ID pRMCE\DV1 standard; circular DNA; UNA; 8893 BP. DT 12-DEC-2013 DE LMBP 8870. CC Accession number: LMBP 8870 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 23-DEC-2019 CC NGS data implemented CC CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC ORIGDB|GenBank CC VNTDATE|-13135037| CC VNTDBDATE|-13135051| CC LSOWNER| CC VNTAUTHORNAME|Demo User| CC VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTOAUTHORTEL|0032-9-33-13-843| CC VNTOAUTHOREML|bccm.genecorner@UGent.be| CC VNTOAUTHORWWW|www.genecorner.ugent.be| CC VNTOAUTHORAD1|Ghent University| CC VNTOAUTHORAD2|Technologiepark 927| CC VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_feature 35..277 FT /vntifkey="21" FT /label=chicken\ß-globin\5'HS4\insulator\core\sequence FT misc_feature 278..520 FT /vntifkey="21" FT /label=chicken\ß-globin\5'HS4\insulator\core\sequence FT misc_feature 1070..1117 FT /vntifkey="21" FT /label=FRT\mutant FT CDS complement(2323..3183) FT /vntifkey="4" FT /label=ampicillin\resistance FT misc_feature 3801..3848 FT /vntifkey="21" FT /label=FRT FT misc_feature complement(5444..5477) FT /vntifkey="21" FT /label=loxP FT attenuator complement(5533..5657) FT /vntifkey="0" FT /label=attR1 FT attenuator 7113..7237 FT /vntifkey="0" FT /label=attR2 FT RBS 7281..7861 FT /vntifkey="32" FT /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES) FT CDS 7874..8593 FT /vntifkey="4" FT /label=EGFP FT promoter complement(1205..1288) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin complement(1380..1782) FT /vntifkey="33" FT /label=pMB1\ori FT misc_feature complement(4031..4064) FT /vntifkey="21" FT /label=loxP FT promoter 539..1049 FT /vntifkey="29" FT /label=mPGK1\promoter FT terminator 4751..4847 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(4751..4847) FT /vntifkey="43" FT /label=SV40\polyA\late FT terminator 5005..5101 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator 5259..5355 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(5005..5101) FT /vntifkey="43" FT /label=SV40\polyA\late FT terminator complement(5259..5355) FT /vntifkey="43" FT /label=SV40\polyA\late FT promoter 5665..5717 FT /vntifkey="30" FT /label=lacUV5\promoter FT CDS 5766..6425 FT /vntifkey="4" FT /label=chloramphenicol\resistance FT CDS 6767..7072 FT /vntifkey="4" FT /label=ccdB FT terminator 8624..8848 FT /vntifkey="43" FT /label=BGH\polyA FT misc_feature 3865..3968 FT /vntifkey="21" FT /label=SA SQ Sequence 8893 BP; 2181 A; 2317 C; 2214 G; 2181 t; cgcgccgggc cctcgcgact cgaggaataa attcgagctc acggggacag ccccccccca 60 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg 120 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc 180 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg 240 agcctgcaga cacctggggg atacggggaa aaagcttgag ctcacgggga cagccccccc 300 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc 360 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac 420 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct 480 ttgagcctgc agacacctgg gggatacggg gaaaaagctt gtcgacgaat aaattcaatt 540 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 600 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 660 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 720 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 780 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 840 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 900 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 960 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 1020 ctcttcctca tctccgggcc tttcgacctg caatcgccgc caccatggag aagttcctat 1080 tccgaagttc ctattcttca aaaggtatag gaacttccga tcgggatccc caatcctgca 1140 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 1200 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 1260 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 1320 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 1380 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 1440 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 1500 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 1560 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 1620 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 1680 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 1740 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 1800 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 1860 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 1920 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 1980 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 2040 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 2100 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 2160 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 2220 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 2280 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 2340 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 2400 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 2460 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 2520 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 2580 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 2640 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 2700 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 2760 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 2820 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 2880 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 2940 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 3000 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 3060 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 3120 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 3180 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 3240 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 3300 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 3360 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 3420 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 3480 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 3540 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 3600 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 3660 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 3720 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 3780 attccacaaa agtgcgatcg gaagttccta ttccgaagtt cctattctct agaaagtata 3840 ggaacttctt aattaaggga tctgtagggc gcagtagtcc agggtttcct tgatgatgtc 3900 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc 3960 tttccagtgg ggatcgacgg tatcgtagag tcgaggccgc tctagaacta gtggatccgg 4020 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgacctg 4080 caggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta 4140 gcagccccgc tggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat 4200 ccaccggtag cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc 4260 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat 4320 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg 4380 ggtaggatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc 4440 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt 4500 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca 4560 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat 4620 cagaagcttg cagatctgcg actctagagg atctgcgact ctagaggatc ataatcagcc 4680 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 4740 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 4800 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 4860 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctgc gactctagag 4920 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 4980 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 5040 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 5100 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 5160 ctgcgactct agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta 5220 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt 5280 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 5340 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 5400 tatcatgtct ggatccccat caagctgatc cggaaccctt aatataactt cgtataatgt 5460 atgctatacg aagttattag gtccctcgac ctgcagccca agctagctta tcgataccgt 5520 cgacctcgaa tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat 5580 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa 5640 catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg 5700 gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag 5760 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta 5820 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc 5880 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct 5940 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag 6000 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa 6060 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca 6120 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta 6180 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa 6240 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc 6300 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct 6360 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg 6420 cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg 6480 atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta 6540 tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc 6600 atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt 6660 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt 6720 attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt 6780 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat 6840 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa 6900 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac 6960 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca 7020 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg 7080 ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac 7140 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat 7200 tttacgtttc tcgttcagct ttcttgtaca aagtggtgat tcgaggtcga cggtatcgat 7260 aagcttgata tcgaattccg cccccccccc tctccctccc ccccccctaa cgttactggc 7320 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 7380 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 7440 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 7500 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 7560 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 7620 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 7680 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 7740 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 7800 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa 7860 tatggccaca accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 7920 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 7980 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 8040 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 8100 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 8160 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 8220 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 8280 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 8340 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 8400 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 8460 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 8520 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 8580 gctgtacaag taaagcggcc gcgagctcgc tgatcagcct cgactgtgcc ttctagttgc 8640 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 8700 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 8760 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 8820 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg 8880 atcctctagt tgg 8893 //