LOCUS       pEF-BOS-CAS3AB 8035 bp dsDNA  Circular
DEFINITION  LMBP 8871.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     rep_origin      3955..4411
                     /note="phage origin"
                     /label=M13
     polyA_signal    complement(4666..4682)
                     /note="HG-CSF"
                     /label=POLYA
     promoter        complement(12..1196)
                     /note="Human EF1 a promoter"
                     /label=HEF1A
     promoter        1337..1508
                     /note="promoter"
                     /label=SV40E
     rep_origin      1305..1380
                     /note="fragment containing bidirectional ori"
                     /label=SV40
     CDS             complement(1532..1569)
                     /note="RBS lacZ gene"
                     /label=RBS
     promoter        complement(1570..1653)
                     /note="lac promoter"
                     /label=LAC
     rep_origin      complement(1745..2145)
                     /note="origin plasmid"
                     /label=ORI
     CDS             complement(2688..3548)
                     /note="antibiotic resistance gene"
                     /label=AMP
     5'UTR           complement(5054..5322)
                     /label=5'UTR
     3'UTR           complement(7909..8013)
                     /label=3'UTR
     CDS             complement(5323..7908)
                     /label=HCAS
     3'clip          complement(5323..5325)
                     /label=STOP
     5'clip          complement(7906..7908)
                     /note="Start codon"
                     /label=START
ORIGIN
        1 ctagagaatt cctcacgaca cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg 
       61 cttgagaatg aaccaagatc caaactcaaa aagggcaaat tccaaggaga attacatcaa 
      121 gtgccaagct ggcctaactt cagtctccac ccactcagtg tggggaaact ccatcgcata 
      181 aaacccctcc ccccaaccta aagacgacgt actccaaaag ctcgagaact aatcgaggtg 
      241 cctggacggc gcccggtact ccgtggagtc acatgaagcg acggctgagg acggaaaggc 
      301 ccttttcctt tgtgtgggtg actcacccgc ccgctctccc gagcgccgcg tcctccattt 
      361 tgagctccct gcagcagggc cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga 
      421 ccgggccagc cttgccgccc agggcggggc gatacacggc ggcgcgaggc caggcaccag 
      481 agcaggccgg ccagcttgag actacccccg tccgattctc ggtggccgcg ctcgcaggcc 
      541 ccgcctcgcc gaacatgtgc gctgggacgc acgggccccg tcgccgcccg cggccccaaa 
      601 aaccgaaata ccagtgtgca gatcttggcc cgcatttaca agactatctt gccagaaaaa 
      661 aagcgtcgca gcaggtcatc aaaaatttta aatggctaga gacttatcga aagcagcgag 
      721 acaggcgcga aggtgccacc agattcgcac gcggcggccc cagcgcccag gccaggcctc 
      781 aactcaagca cgaggcgaag gggctcctta agcgcaaggc ctcgaactct cccacccact 
      841 tccaacccga agctcgggat caagaatcac gtactgcagc caggtggaag taattcaagg 
      901 cacgcaaggg ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac 
      961 ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat 
     1021 atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca 
     1081 gtttaccccg cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt 
     1141 ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc ctcacgaggc 
     1201 actcctttca agacctagaa ggtccattag ctgcaaagat tcctctctgt ttaaaacttt 
     1261 atccatcttt gcaaagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact 
     1321 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc 
     1381 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta 
     1441 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 
     1501 gggagcctaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 
     1561 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 
     1621 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 
     1681 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 
     1741 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 
     1801 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 
     1861 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     1921 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
     1981 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     2041 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     2101 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 
     2161 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     2221 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     2281 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     2341 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 
     2401 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     2461 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     2521 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     2581 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     2641 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     2701 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     2761 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     2821 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     2881 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     2941 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     3001 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     3061 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
     3121 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     3181 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     3241 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
     3301 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
     3361 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
     3421 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
     3481 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
     3541 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     3601 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     3661 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 
     3721 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc 
     3781 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga 
     3841 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg 
     3901 gcatcagagc agattgtact gagagtgcac catgctcgtc aaagcaacca tagtacgcgc 
     3961 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 
     4021 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 
     4081 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 
     4141 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc 
     4201 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 
     4261 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga 
     4321 ttttgccgat ttcggaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt 
     4381 ggaccgaatt ttaacaaaat attaacgttt tgcggtgtga aataccgcac agatgcgtaa 
     4441 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 
     4501 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 
     4561 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 
     4621 aattcttttt tttttttttt tttttttttt tttttttttt tttttggagg caaacacttt 
     4681 attatcctga agttcagtta caatgtgaca gtggagggga cactggtgag tggggggtga 
     4741 agaggtggag ggtgacagtg gagcttcctt tcacacacag gcctgacatg attcctcctg 
     4801 tctgctccct cccacatccc cagtccccat ccagcaaggc aaatgtccag gcacctgcct 
     4861 ggccctaaaa agagtcacag tcctgaccct cgtgggggca gggcaggtgt cccgaggcca 
     4921 cccagaaaaa caggagacac gtcggtgatg ttcgggagtc aaaccatgtc ccaaaagtct 
     4981 taagaagaaa aacgagattc cccagcaaat tcgtgggacc ccagggccat gcctcccagc 
     5041 tctggaattc gtcgcagttt ctcaaaatat gcaaataatt gagtcaggtt tcttgggaga 
     5101 aaaatggagg gaatggggga gggggaggcg ttgagacaat taaacttcac aggaagaagt 
     5161 tgggagttga tggaaagaag gcagccactt agggctgtaa gggcaggagg cagcctggca 
     5221 gttggcacaa tagtccagcg aagaaaaagg aaaatcatca gtcaatttca ccaccaaaaa 
     5281 ataaaagtac atataagccc agatggaacg gagatagtgg tgctaaatct tctgcatgga 
     5341 ggggtaagac acttggcccc cctcatcatc caaaaccaac acatccaact cttcctccag 
     5401 agattcctgc ccctcgtcct gcatcaaggg tgttgttccc ttcaatggct ccatgtcgcc 
     5461 tagatccccc gatcgatcca gtgttctatt gtggtctcct ctctcatttg gtgtggaata 
     5521 gttgttatct agtgattttg tgtttgatcc catattgctc atctgaattt cttcccgatc 
     5581 aggtttctta tctgattttt ggttccggtc aatgagaggg agagtactat catcatatga 
     5641 atgactgctc tggcttcggg aagcattgtt tagattcacc tgaaagtctg atttcttcca 
     5701 tccttctttt tccagtggct tccgcagttc cttatatccc cagattgtct gtaatacaag 
     5761 tgctgctgct cgaacttctt tttctgagcg gttccctgat ttgttgatca acaccagctt 
     5821 ctcaataccc tgtgtctctc gaagcttttt ggcagcctcc aagttctcag cgataacctc 
     5881 gttgatagtg ttcaaaatag agatgacagt gtcctcagag aaattccaag aggagttctg 
     5941 ctgtcctcct ggcagattct ttaccaagtt aggaatagca tgtttaccaa ttaattcttt 
     6001 gttgcgagca tccacagcca ggtttctcag tgctccagat gcagctttca ccacccgttc 
     6061 atgttcatta gtcaggaggt cagctatggc agaaagagcc ttctcttgac gcagagcaga 
     6121 gcggatgtat cgaccatacg tccagcgccc agcacacaag ttctggatag ctccagctga 
     6181 ggcttctagg atggcaggag tcttgctctc cttaagaagt gagatgtata tccgaaccac 
     6241 ctctggctga aataagagct catagcctcg agctggactc gttcttttag ggaaatccac 
     6301 tgtatcgttt gctggatcct ctataggttt tttccctttg cccttcttgg ccccaaagca 
     6361 actggcagca tgtggcccag tattgttggc aacattggga gctgcctctt ggtaacgctc 
     6421 tgcctgtggg atctcccggt gaacttgata tgataagttc cgaagaaggc aaacacagtt 
     6481 ctctacaagc ttgctgtctg aatccttctg cccaatctca gcctgaacaa tgaaaatgag 
     6541 ggcatcaact aaaccatcac attcccgaag tttccggcga gcttcactcc tctctgagct 
     6601 tacattccta aggcagccag ctgtgttggt gagcaccgat tcccactcaa tatggcgtgg 
     6661 cttacagtct tcattaggtt cccgctccca accagaatga ggaatgatca cttcatctgt 
     6721 caaggcatgc agtgcatggt ccacaatctc cattttgatt gagtcatggg atgaaagatt 
     6781 ccacagggtt ccggtaataa cttcagtaag gtccatatca cgagcctttc gaagcaatcg 
     6841 cacaagggca ggcacaccat cacagttttt tatggcaatc ttgttatcct ggtcacgtcc 
     6901 aaaagagata ttcttgagag ctccacaggc tccaaggtgc acttcctttt tgggatggtc 
     6961 taacaatccc accagtactg ggatgccctt gagcttccgc acgtcagtct tcaccttgtc 
     7021 attgcggtag cataagtgtt gcaggtatgc agctgcattg gacttgacag catccaagcg 
     7081 gaatccaagc atggcgatca cctctggcag ctctggctgt ctccaattag gaggtggagg 
     7141 ccctcctttg cgcaggctat ccaagcttgc taaacttcct cgctcatgct gggccaaagg 
     7201 agcccagtag tattgatccg atggcacctc ctcaccaatc atgtcttcat agctcctgag 
     7261 gcgccgacga gggtcagagg gtgtcccagt ccgacgggca gtgccataat cagacatcat 
     7321 accataatcc aggtcatcat agcccatact acgctggtca tcctctagcc cataaggctc 
     7381 tggatgaaag cgatgcagat ccacgctgct cccacctacc cgaacctggg gttggggccc 
     7441 atacacatcc tgtctactag gtgcccggta gccttccatg ctgggcctat accgctcctc 
     7501 aatgcgggtc acccgggaca gactgccata gttatcactg ccacctggat aaccatcttc 
     7561 atagtggcga ctataaccat caggagggta gtggaagttc ctaggaaggg tagcagtgcc 
     7621 agcttgcccc acatagggac caggtccccc attgccattc ttgcggaaat cacgacccaa 
     7681 agtctggata tagttgttag aaactgatga agcatccaca ggcaacccgt ctggtcccat 
     7741 agcgactggc tgtactgtcc gtgttgtcac agtcttcact actttcttga ccgtggtctc 
     7801 tgtgcgccga gtggtcccat catctgaggt ctccacagag actacagaca tggctccctc 
     7861 aggatcctcc tccgtgtagg tctccacaat ctgccccggc tcctgcatcc tggggatggt 
     7921 gctatataga aggtgactgt gatcctgggg tccgttgagt ttcaaatctg aaaatttctg 
     7981 tctttcaagg tcagcatcgc ccacaaaccg gccgacctcg agggatccgg gccct 
//
