LOCUS       pJ3-Ecad-C-Myc 8167 bp dsDNA    Circular
DEFINITION  LMBP 8876.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     CDS             complement(7709..2170)
                     /note="artificial EcoRI ( linker )"
                     /label=HSECAD
     3'clip          complement(7676..7678)
                     /note="stop  (h Ecadherine + c-myc-tag)"
                     /label=STOP
     CDS             7679..7708
                     /note="c-myc-tag"
                     /label=c-MYC
ORIGIN
        1 ggcggcccct tcacagtcac acacgctgac ctctaaggtg gtcacttggt ctttattctg 
       61 gttatccatg agcttgagat tgattttgta gtcacccacc tctaaggcca tctttggctt 
      121 caaaatgata gattcttggg ttgggtcgtt gtactgaatg gtccagttgg cactcgcccc 
      181 gtgtgttagt tctgctgtga agggagatgt attgggagga aggtctgcat caatgatgtt 
      241 tatgacctga ggctttggat tcctctcaca gaagaatata gttcgaggtt ctggtatggg 
      301 ggcgttgtca ttcacatcag acaggatcag cagaagtgtc cctgttccag tagcaactgg 
      361 agaaccattg tctgtagcta tgattagggc tgtgtacgtg ctgttcttca cgtgctcaaa 
      421 atcctccctg tccagctcag cccgagtgga aatggcacca gtgtccggat taatctccag 
      481 ccagttggca gtgtctctcc aaatccgata tgttattttc tgttccataa atgtgtctgg 
      541 ctcctgggca gtgtaggatg tgatttcctg gcccacgcca aagtcctcgg acacttccac 
      601 tctcttttca ggaggcacaa agatgggggc ttcattcaca tccagcacat ccacggtgac 
      661 ggtggctgtg gaggtggtga gagagacctc aaaaggtacc acattcgtca ctgctacgtg 
      721 tagaatgtac tgctgcttgg cctcaaaatc caagcccttt gctgttttca aaatgccatc 
      781 gttgttcact ggatttgtgg tgacgacaaa ttgtccacca tcatcattca atatggtgta 
      841 tacagcctcc cacgctgggg tattgggggc atcagcatca gtcactttca gtgtggtgat 
      901 tacgacgtta gcctcgttct caggcacctg acccttgtac gtggtgggat tgaagatcgg 
      961 aggattatcg ttggtgtcag tgactgtgat cacagctgtt gctgttgtgc ttaacccctc 
     1021 accttgaagg tcagcagctt gaaccaccag ggtatacgta gggaaactct ctcggtccag 
     1081 cccagtggtg accacactga tgactcctgt gttcctgtta atggtgaaca tatttttgtc 
     1141 agggagctca ggatcttggc tgaggatggt gtaagcgatg gcggcattgt aggtgttcac 
     1201 atcatcgtcc gcgtctgtgg ctgtgacctc catcacagag gttcctggaa gagcaccttc 
     1261 catgacagac cccttaaaga cctcctgggt gaattcgggc ttgttgtcat tctgatcggt 
     1321 taccgtgatc aaaatctcca ttggatcctc aactgcattc ccgttggatg acacagcgtg 
     1381 agagaagaga gtgtatgtgg caatgcgttc tctatccaga ggctctgtca ccttcagcca 
     1441 tcctgtttct ctttcaataa taaagacacc aacagggggt gtgtcagctc cttggccagt 
     1501 gatgctgtag aaaaccttgc cttctttgtc tttgttggat ttgatctgaa ccaggttttt 
     1561 aggaaatggg cctttttcat tttctgggca gctgatggga ggaataaccc agtctctctt 
     1621 ctgtcttctg aggccaggag aggagttggg aaatgtgagc aattctgctt ggattccaga 
     1681 aacggaggcc tgatggggcg gggggcggtg gtggtgcccc actgtattca gcgtgacttt 
     1741 ggtggaaaac tttctgtagg tggagtccca ggcgtagacc aagaaatgga tctgtgggtt 
     1801 atgaaaccgt agaggccttt tgactgtaat cacaccatct gtgcccactt tgaatcgggt 
     1861 gtcgagggaa aaataggctg tcctttgtcg accggtgcaa tcttcaaaat tcactctgcc 
     1921 caggacgcgg cctctctcca ggtggcgccg gggcaccgtg aacgtgtagc tctcggcgtc 
     1981 aaagccaggg tggcagggct ccggctcctg gcagagccaa gaggagacct gcagcagcag 
     2041 cagcagcgcc gagaggctgc ggctccaagg gcccatggct ggccggggac gccgagcgag 
     2101 ggcaggcgcc gggtgcggtc gggtcgggcc gggctggagc gggctggagt ctgaactgac 
     2161 ttccgcaagc acctccggaa ttcgagcttt ttgcaaaagc ctaggcctcc aaaaaagcct 
     2221 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa 
     2281 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg 
     2341 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt 
     2401 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg 
     2461 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca 
     2521 ttccacagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 
     2581 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 
     2641 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc 
     2701 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata 
     2761 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg 
     2821 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
     2881 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 
     2941 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
     3001 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
     3061 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     3121 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     3181 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     3241 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     3301 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     3361 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     3421 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     3481 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     3541 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     3601 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     3661 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     3721 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     3781 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     3841 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 
     3901 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     3961 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     4021 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg 
     4081 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     4141 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
     4201 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     4261 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     4321 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata 
     4381 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     4441 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     4501 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     4561 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     4621 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     4681 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     4741 cacctgacgt gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag 
     4801 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac 
     4861 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt 
     4921 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc 
     4981 tgattatgat ctctagtcaa ggcactatac atcaaatatt ccttattaac ccctttacaa 
     5041 attaaaaagc taaaggtaca caatttttga gcatagttat taatagcaga cactctatgc 
     5101 ctgtgtggag taagaaaaaa cagtatgtta tgattataac tgttatgcct acttataaag 
     5161 gttacagaat atttttccat aattttcttg tatagcagtg cagctttttc ctttgtggtg 
     5221 taaatagcaa agcaagcaag agttctatta ctaaacacag catgactcaa aaaacttagc 
     5281 aattctgaag gaaagtcctt ggggtcttct acctttctct tcttttttgg aggagtagaa 
     5341 tgttgagagt cagcagtagc ctcatcatca ctagatggca tttcttctga gcaaaacagg 
     5401 ttttcctcat taaaggcatt ccaccactgc tcccattcat cagttccata ggttggaatc 
     5461 taaaatacac aaacaattag aatcagtagt ttaacacatt atacacttaa aaattttata 
     5521 tttaccttag agctttaaat ctctgtaggt agtttgtcca attatgtcac accacagaag 
     5581 taaggttcct tcacaaagat ctggtaccat cgatgaattc gagctcgccc ggggatcctc 
     5641 tagaaacaaa attgtttaat aaaatttata aaaatgcatc tttgagaata cttttctcag 
     5701 cttgaattgt tttccttttc cacccccaaa gaaaatacac aattatcagc acccacacat 
     5761 gtatacactc aaaactacag tgacattctc tacacagaac tatattcgat atagcttgaa 
     5821 ctgccgaaaa atcaagacaa ttccaaaaag tgattgcagg gttgattttt ttctccaaaa 
     5881 cacttgagaa acagtaaagc tatttcaaca aaagtctttt ctttgattgt caaaagttga 
     5941 aattcacatt taaataaaaa gagatccaaa tcaagatcct cactaccccc tacccctcaa 
     6001 ctaaccccct ttagggccac attttcttct tgctcctaag aaaaaatttg gaattttgaa 
     6061 tattctcggt tttctgtgca cacctggaat tgggcaaatg tgttcagctc agccagcatt 
     6121 ttctgtagac atcatcaaaa gcaggcactt ggggattctg ggctttgagt acaaaccacg 
     6181 gatcttgtgt cagaaacaca tgttgagact cctccattcc ttccagaatt ttcagagatg 
     6241 aggtagaccc acctcaatca tcctcagcat cagtttgcta aattgccagg ctcaatgaca 
     6301 agctctcctg ccatctccaa gcccactttt catagttccg ctctgtcttt ggctgcagca 
     6361 ctttaggcac tattctaagt cctggagtat atcactcttg cttcagagct aaataaacat 
     6421 taatgaacac acttactcag aacaagtcac tggatagctg cccattgcaa gttacatact 
     6481 caggagatga aagagggaag ccattaaagg tcttcagagt agacaatacc tagtcaagat 
     6541 gtggccagac aaagacacaa acttcttacc ctaaaagagc ccaataattt ctgcatcaga 
     6601 gaactcctat cttgggcaaa gcaactgaat tcaggagtga gagttgatat taaaacatgg 
     6661 aggctgggca cagcagctca tgcccacaat cccagcactt tggggggcca aggcagaagg 
     6721 attgcttgag gccaggagtt taagaccagc ttgggcaaca tagcaagacc ccatctgtac 
     6781 aaaaaaataa aaaggaggct ggtgggagaa ctgcttgagc cccagagttt gaggttacag 
     6841 tgagctatga tcacatcact gcatcccagg cctgggcgat ggagcgaaac tgtctcttaa 
     6901 aaaatggcag ggagttgggg agctgggcag gtgcagtggc tcatgtctgt aatcccaata 
     6961 ctctgggagg ccaagatggg aggatcactt gagcccagga gtttgagacc agcctgggta 
     7021 acacagggag accccgtctc aaatatttaa aaaattagtc atgcgtagtg gtgcatgcct 
     7081 gtggtcccag ctacttggga ggctgaggtg caaggatagc ttgagcctgg gaggacaagg 
     7141 ctgcagtgag ctgtgattgc accactgcac tccagcctgg ccgatagaat gagaccctgt 
     7201 cttaaaaaaa aaaaaaaagg cagagggaca caccagtgta gtaatgagca gaagcacaca 
     7261 aatttttttt aaaaaattat atacacaaaa caacagaact agagtagctg acttctcccc 
     7321 ttctttttaa aagtgagaca aaattcctta agaaaatctg caaggtgctg ggtgaacctt 
     7381 ctgatgctaa agttgttctt ctggaatatt aaaatttggg tatcttttgg acatcaccac 
     7441 catgtaaaga gtgatgggaa aaaaaaaaaa gctttaagaa ataagtcgaa acttttctaa 
     7501 cacaaactta ttagagctat aaagtggaga gagtataaag ctatactaac tgcatcacta 
     7561 accagtctct tttttttccc cagaaactca tctcaaggga agggagctga aaaaccacca 
     7621 gcaacgtgat ttctgcattt cccagcacat gggtctgggg cccgcctctc tcgagttaca 
     7681 gatcctcttc agagatcagt ttttgttcca gactagcagc ttcggaaccg cttccttcat 
     7741 agtcaaacac gagcagagaa tcataaggcg gggctgtggg gtcagtatca gccgctttca 
     7801 gattttcatc aataaaattt ccaatttcat cgggattggc agggcgggga agataccggg 
     7861 ggacactcat gagggttggt gcaacgtcgt tacgagtcac ttcaggccga gcgtccaggc 
     7921 ccctgtgcag ctggctcaag tcaaagtcct ggtcctcttc tccgcctcct tcttcatcat 
     7981 agtaataaac gttgtcccgg gtgtcatcct ctgggggcag taagggctct ttgaccaccg 
     8041 ctctcctccg aagaaacagc aagagcagca gaatcagaat tagcaaagca agaattcctc 
     8101 caagaatccc cagaatggca ggaatttgca atcctgcttc gacaggctgt gccttcctac 
     8161 agacgcc 
//
