LOCUS       peGADT7-haEctn    10887 bp    dsDNA    circular
DEFINITION  LMBP 8890.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        5984..7456
                     /label=F.L.\ADH1\promoter
     CDS             7478..7876
                     /label=GAL4AD\with\SV40\NLS
     promoter        7882..7904
                     /label=T7\RNA\pol.\promoter
     CDS             7919..7945
                     /label=HA\epitope\tag
     terminator      266..591
                     /label=ADH1\transcription\terminator
     CDS             complement(709..1800)
                     /label=LEU2
     CDS             complement(3561..4418)
                     /label=CARB
     primer_bind     7795..7824
                     /label=FVR174
     primer_bind     complement(107..126)
                     /label=FVR192
     5'clip          7976..8000
                     /label=attB1
     primer_bind     7976..8000
                     /label=FVR\2923
     3'clip          10863..10887
                     /label=attB2
     primer_bind     10863..10887
                     /label=FVR2924
ORIGIN
        1 tgatgatcca tcgagctcga gctgcagatg aatcgtagat actgaaaaac cccgcaagtt 
       61 cacttcaact gtgcatcgtg caccatctca atttctttca tttatacatc gttttgcctt 
      121 cttttatgta actatactcc tctaagtttc aatcttggcc atgtaacctc tgatctatag 
      181 aattttttaa atgactagaa ttaatgccca tctttttttt ggacctaaat tcttcatgaa 
      241 aatatattac gagggcttat tcagaagctt tggacttctt cgccagaggt ttggtcaagt 
      301 ctccaatcaa ggttgtcggc ttgtctacct tgccagaaat ttacgaaaag atggaaaagg 
      361 gtcaaatcgt tggtagatac gttgttgaca cttctaaata agcgaatttc ttatgattta 
      421 tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact 
      481 cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc 
      541 tttctcaggt atagcatgag gtcgctctta ttgaccacac ctctaccggc cggtcgaaat 
      601 tcccctaccc tatgaacata ttccattttg taatttcgtg tcgtttctat tatgaatttc 
      661 atttataaag tttatgtaca aatatcataa aaaaagagaa tctttttaag caaggatttt 
      721 cttaacttct tcggcgacag catcaccgac ttcggtggta ctgttggaac cacctaaatc 
      781 accagttctg atacctgcat ccaaaacctt tttaactgca tcttcaatgg ccttaccttc 
      841 ttcaggcaag ttcaatgaca atttcaacat cattgcagca gacaagatag tggcgatagg 
      901 gttgacctta ttctttggca aatctggagc agaaccgtgg catggttcgt acaaaccaaa 
      961 tgcggtgttc ttgtctggca aagaggccaa ggacgcagat ggcaacaaac ccaaggaacc 
     1021 tgggataacg gaggcttcat cggagatgat atcaccaaac atgttgctgg tgattataat 
     1081 accatttagg tgggttgggt tcttaactag gatcatggcg gcagaatcaa tcaattgatg 
     1141 ttgaaccttc aatgtaggaa attcgttctt gatggtttcc tccacagttt ttctccataa 
     1201 tcttgaagag gccaaaacat tagctttatc caaggaccaa ataggcaatg gtggctcatg 
     1261 ttgtagggcc atgaaagcgg ccattcttgt gattctttgc acttctggaa cggtgtattg 
     1321 ttcactatcc caagcgacac catcaccatc gtcttccttt ctcttaccaa agtaaatacc 
     1381 tcccactaat tctctgacaa caacgaagtc agtaccttta gcaaattgtg gcttgattgg 
     1441 agataagtct aaaagagagt cggatgcaaa gttacatggt cttaagttgg cgtacaattg 
     1501 aagttcttta cggattttta gtaaaccttg ttcaggtcta acactacctg taccccattt 
     1561 aggaccaccc acagcaccta acaaaacggc atcaaccttc ttggaggctt ccagcgcctc 
     1621 atctggaagt gggacacctg tagcgtcgat agcagcacca ccaattaaat gattttcgaa 
     1681 atcgaacttg acattggaac gaacatcaga aatagcttta agaaccttaa tggcttcggc 
     1741 tgtgatttct tgaccaacgt ggtcacctgg caaaacgacg atcttcttag gggcagacat 
     1801 tagaatggta tatccttgaa atatatatat atattgctga aatgtaaaag gtaagaaaag 
     1861 ttagaaagta agacgattgc taaccaccta ttggaaaaaa caataggtcc ttaaataata 
     1921 ttgtcaactt caagtattgt gatgcaagca tttagtcatg aacgcttctc tattctatat 
     1981 gaaaagccgg ttccggcgct ctcacctttc ctttttctcc caatttttca gttgaaaaag 
     2041 gtatatgcgt caggcgacct ctgaaattaa caaaaaattt ccagtcatcg aatttgattc 
     2101 tgtgcgatag cgcccctgtg tgttctcgtt atgttgagga aaaaaataat ggttgctaag 
     2161 agattcgaac tcttgcatct tacgatacct gagtattccc acagttgggg atctcgactc 
     2221 tagctagagg atcaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 
     2281 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 
     2341 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 
     2401 cctgtcgtgc cagctgataa cttcgtataa tgtatgctat acgaagttat taggtctgaa 
     2461 gaggagttta cgtccagcca agctagcttg gctgcaggtc gagcggccgc gatccggaac 
     2521 ccttaatata acttcgtata atgtatgcta tacgaagtta tcagctgcat taatgaatcg 
     2581 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 
     2641 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 
     2701 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 
     2761 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 
     2821 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 
     2881 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 
     2941 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 
     3001 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 
     3061 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
     3121 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 
     3181 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 
     3241 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 
     3301 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 
     3361 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 
     3421 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 
     3481 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 
     3541 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 
     3601 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 
     3661 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 
     3721 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 
     3781 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 
     3841 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 
     3901 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 
     3961 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 
     4021 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 
     4081 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 
     4141 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 
     4201 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 
     4261 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 
     4321 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 
     4381 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 
     4441 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 
     4501 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 
     4561 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 
     4621 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
     4681 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
     4741 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
     4801 accataacgc atttaagcat aaacacgcac tatgccgttc ttctcatgta tatatatata 
     4861 caggcaacac gcagatatag gtgcgacgtg aacagtgagc tgtatgtgcg cagctcgcgt 
     4921 tgcattttcg gaagcgctcg ttttcggaaa cgctttgaag ttcctattcc gaagttccta 
     4981 ttctctagct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa 
     5041 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga 
     5101 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat 
     5161 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct 
     5221 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta 
     5281 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag 
     5341 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc 
     5401 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct 
     5461 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt 
     5521 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg 
     5581 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa 
     5641 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa 
     5701 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta 
     5761 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt 
     5821 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt 
     5881 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt 
     5941 gttctacaaa atgaagcaca gatgcttcgt tgctcggtgc atgcctgcag gtcgagatcc 
     6001 gggatcgaag aaatgatggt aaatgaaata ggaaatcaag gagcatgaag gcaaaagaca 
     6061 aatataaggg tcgaacgaaa aataaagtga aaagtgttga tatgatgtat ttggctttgc 
     6121 ggcgccgaaa aaacgagttt acgcaattgc acaatcatgc tgactctgtg gcggacccgc 
     6181 gctcttgccg gcccggcgat aacgctgggc gtgaggctgt gcccggcgga gttttttgcg 
     6241 cctgcatttt ccaaggttta ccctgcgcta aggggcgaga ttggagaagc aataagaatg 
     6301 ccggttgggg ttgcgatgat gacgaccacg acaactggtg tcattattta agttgccgaa 
     6361 agaacctgag tgcatttgca acatgagtat actagaagaa tgagccaaga cttgcgagac 
     6421 gcgagtttgc cggtggtgcg aacaatagag cgaccatgac cttgaaggtg agacgcgcat 
     6481 aaccgctaga gtactttgaa gaggaaacag caatagggtt gctaccagta taaatagaca 
     6541 ggtacataca acactggaaa tggttgtctg tttgagtacg ctttcaattc atttgggtgt 
     6601 gcactttatt atgttacaat atggaaggga actttacact tctcctatgc acatatatta 
     6661 attaaagtcc aatgctagta gagaaggggg gtaacacccc tccgcgctct tttccgattt 
     6721 ttttctaaac cgtggaatat ttcggatatc cttttgttgt ttccgggtgt acaatatgga 
     6781 cttcctcttt tctggcaacc aaacccatac atcgggattc ctataatacc ttcgttggtc 
     6841 tccctaacat gtaggtggcg gaggggagat atacaataga acagatacca gacaagacat 
     6901 aatgggctaa acaagactac accaattaca ctgcctcatt gatggtggta cataacgaac 
     6961 taatactgta gccctagact tgatagccat catcatatcg aagtttcact accctttttc 
     7021 catttgccat ctattgaagt aataataggc gcatgcaact tcttttcttt ttttttcttt 
     7081 tctctctccc ccgttgttgt ctcaccatat ccgcaatgac aaaaaaaatg atggaagaca 
     7141 ctaaaggaaa aaattaacga caaagacagc accaacagat gtcgttgttc cagagctgat 
     7201 gaggggtatc tcgaagcaca cgaaactttt tccttccttc attcacgcac actactctct 
     7261 aatgagcaac ggtatacggc cttccttcca gttacttgaa tttgaaataa aaaaaagttt 
     7321 gctgtcttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 
     7381 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 
     7441 catacaatca actccaagct ttgcaaagat ggataaagcg gaattaattc ccgagcctcc 
     7501 aaaaaagaag agaaaggtcg aattgggtac cgccgccaat tttaatcaaa gtgggaatat 
     7561 tgctgatagc tcattgtcct tcactttcac taacagtagc aacggtccga acctcataac 
     7621 aactcaaaca aattctcaag cgctttcaca accaattgcc tcctctaacg ttcatgataa 
     7681 cttcatgaat aatgaaatca cggctagtaa aattgatgat ggtaataatt caaaaccact 
     7741 gtcacctggt tggacggacc aaactgcgta taacgcgttt ggaatcacta cagggatgtt 
     7801 taataccact acaatggatg atgtatataa ctatctattc gatgatgaag ataccccacc 
     7861 aaacccaaaa aaagagatct ttaatacgac tcactatagg gcgagcgccg ccatggagta 
     7921 cccatacgac gtaccagatt acgctcatat ggccatggag gccagtgaat tatcaacaag 
     7981 tttgtacaaa aaagcaggct tgactgctgt ccatgcaggc aacataaact tcaagtggga 
     8041 tcctaaaagt ctagagatca ggactctggc agttgagaga ctgttggagc ctcttgttac 
     8101 acaggttaca acccttgtaa acaccaatag taaagggccc tctaataaga agagaggtcg 
     8161 ttctaagaag gcccatgttt tggctgcatc tgttgaacaa gcaactgaga atttcttgga 
     8221 gaagggggat aaaattgcga aggagagcca gtttctcaag gaggagcttg tggctgctgt 
     8281 agaagatgtt cgaaaacaag gtgatttgat gaaggctgct gcaggagagt tcgcagatga 
     8341 tccctgctct tctgtgaagc gaggcaacat ggttcgggca gctcgagctt tgctctctgc 
     8401 tgttacccgg ttgctgattt tggctgacat ggcagatgtc tacaaattac ttgttcagct 
     8461 gaaagttgtg gaagatggta tcttgaagtt gaggaatgct ggcaatgaac aagacttagg 
     8521 aatccagtat aaagccctaa aacctgaagt ggataagctg aacattatgg cagccaaaag 
     8581 acaacaggaa ttgaaagatg ttggccatcg tgatcagatg gctgcagcta gaggaatcct 
     8641 gcagaagaac gttccgatcc tctatactgc atcccaggca tgcctacagc accctgatgt 
     8701 cgcagcctat aaggccaacc gggacctgat atacaagcag ctgcagcagg cggtcacagg 
     8761 catttccaat gcagcccagg ccactgcctc agacgatgcc tcacagcacc agggtggagg 
     8821 aggaggagaa ctggcatatg cactcaataa ctttgacaaa caaatcattg tggacccctt 
     8881 gagcttcagc gaggagcgct ttaggccttc cctggaggag cgtctggaaa gcatcattag 
     8941 tggggctgcc ttgatggccg actcctcctg cacgcgtgat gaccgtcgtg agcgaattgt 
     9001 ggcagagtgt aatgctgtcc gcaaggccct gcaggacctg ctttcggagt acatgggcaa 
     9061 tgctggacgt aaagaaagaa gtgatgcact caattctgca atagataaaa tgaccaagaa 
     9121 gaccagggac ttgcgtagac agctccgcaa agctgtcatg gaccacgttt cagattcttt 
     9181 cctggaaacc aatgttccac ttttggtatt gattgaagct gcaaagaatg gaaatgagaa 
     9241 agaagttaag gagtatgccc aagttttccg tgaacatgcc aacaaattga ttgaggttgc 
     9301 caacttggcc tgttccatct caaataatga agaaggtgta aagcttgttc gaatgtctgc 
     9361 aagccagtta gaagccctct gtcctcaggt tattaatgct gcactggctt tagcagcaaa 
     9421 accacagagt aaactggccc aagagaacat ggatcttttt aaagaacaat gggaaaaaca 
     9481 agtccgtgtt ctcacagatg ctgtcgatga cattacttcc attgatgact tcttggctgt 
     9541 ctcagagaat cacattttgg aagatgtgaa caaatgtgtc attgctctcc aagagaagga 
     9601 tgtggatggc ctggaccgca cagctggtgc aattcgaggc cgggcagccc gggtcattca 
     9661 cgtagtcacc tcagagatgg acaactatga gccaggagtc tacacagaga aggttctgga 
     9721 agccactaag ctgctctcca acacagtcat gccacgtttt actgagcaag tagaagcagc 
     9781 cgtggaagcc ctcagctcgg accctgccca gcccatggat gagaatgagt ttatcgatgc 
     9841 ttcccgcctg gtatatgatg gcatccggga catcaggaaa gcagtgctga tgataaggac 
     9901 ccctgaggag ttggatgact ctgactttga gacagaagat tttgatgtca gaagcaggac 
     9961 gagcgtccag acagaagacg atcagctgat agctggccag agtgcccggg cgatcatggc 
    10021 tcagcttccc caggagcaaa aagcgaagat tgcggaacag gtggccagct tccaggaaga 
    10081 aaagagcaag ctggatgctg aagtgtccaa atgggacgac agtggcaatg acatcattgt 
    10141 gctggccaag cagatgtgca tgattatgat ggagatgaca gactttaccc gaggtaaagg 
    10201 accactcaaa aatacatcgg atgtcatcag tgctgccaag aaaattgctg aggcaggatc 
    10261 caggatggac aagcttggcc gcaccattgc agaccattgc cccgactcgg cttgcaagca 
    10321 ggacctgctg gcctacctgc aacgcatcgc cctctactgc caccatctga acatctgcag 
    10381 caaggtcaag gccgaggtgc agaatctcgg cggggagctt gttgtctctg gggtggacag 
    10441 cgccatgtcc ctgatccagg cagccaagaa cttgatgaat gctgtggtgc agacagtgaa 
    10501 ggcatcctac gtcgcctcta ccaaatacca aaagtcacag ggtatggctt ccctcaacct 
    10561 tcctgctgtg tcatggaaga tgaaggcacc agagaaaaag ccattggtga agagagagaa 
    10621 acaggatgag acacagacca agattaaacg ggcatctcag aagaagcacg tgaacccggt 
    10681 gcaggccctc agcgagttca aagctatgga cagcatctaa gtctgcccag gccggccgcc 
    10741 cccacccctc ggggctcctg aatatcagtc actgttcgtc actcaaatga atttgctaaa 
    10801 tacaacactg atactagatt ccacagggaa atgggcagac tgaaccagtc caggtggtga 
    10861 acacccagct ttcttgtaca aagtggt 
//
