LOCUS       peGBKT7-haECTN    10200 bp    dsDNA    circular
DEFINITION  LMBP 8893.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     5'clip          1202..1226
                     /label=attB1
     primer_bind     1202..1226
                     /label=FVR\2923
     3'clip          4089..4113
                     /label=attB2
     primer_bind     4089..4113
                     /label=FVR2924
     promoter        10124..630
                     /label=truncated\ADH1\promoter
     CDS             656..1096
                     /label=GAL4DNABD
     promoter        1106..1129
                     /label=T7\RNA\polymerase\promoter
     CDS             1142..1174
                     /label=c-Myc\epitope\tag
     terminator      4125..4171
                     /label=T7\transcription\terminator
     terminator      4204..4400
                     /label=ADH1\transcription\terminator
     CDS             complement(6012..6934)
                     /label=KAN
     CDS             8821..9495
                     /label=TRP1
     primer_bind     1048..1067
                     /label=FVR175
     primer_bind     complement(4208..4227)
                     /label=FVR217
ORIGIN
        1 aataccttcg ttggtctccc taacatgtag gtggcggagg ggagatatac aatagaacag 
       61 ataccagaca agacataatg ggctaaacaa gactacacca attacactgc ctcattgatg 
      121 gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt 
      181 ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt 
      241 ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa 
      301 aaaatgatgg aagacactaa aggaaaaaat taacgacaaa gacagcacca acagatgtcg 
      361 ttgttccaga gctgatgagg ggtatctcga agcacacgaa actttttcct tccttcattc 
      421 acgcacacta ctctctaatg agcaacggta tacggccttc cttccagtta cttgaatttg 
      481 aaataaaaaa agtttgctgt cttgctatca agtataaata gacctgcaat tattaatctt 
      541 ttgtttcctc gtcattgttc tcgttccctt tcttccttgt ttctttttct gcacaatatt 
      601 tcaagctata ccaagcatac aatcaactcc aagcttgaag caagcctcct gaaagatgaa 
      661 gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc tcaagtgctc 
      721 caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc gctactctcc 
      781 caaaaccaaa aggtctccgc tgactagggc acatctgaca gaagtggaat caaggctaga 
      841 aagactggaa cagctatttc tactgatttt tcctcgagaa gaccttgaca tgattttgaa 
      901 aatggattct ttacaggata taaaagcatt gttaacagga ttatttgtac aagataatgt 
      961 gaataaagat gccgtcacag atagattggc ttcagtggag actgatatgc ctctaacatt 
     1021 gagacagcat agaataagtg cgacatcatc atcggaagag agtagtaaca aaggtcaaag 
     1081 acagttgact gtatcgccgg aatttgtaat acgactcact atagggcgag ccgccatcat 
     1141 ggaggagcag aagctgatct cagaggagga cctgcatatg gccatggagg ccgaattatc 
     1201 aacaagtttg tacaaaaaag caggcttgac tgctgtccat gcaggcaaca taaacttcaa 
     1261 gtgggatcct aaaagtctag agatcaggac tctggcagtt gagagactgt tggagcctct 
     1321 tgttacacag gttacaaccc ttgtaaacac caatagtaaa gggccctcta ataagaagag 
     1381 aggtcgttct aagaaggccc atgttttggc tgcatctgtt gaacaagcaa ctgagaattt 
     1441 cttggagaag ggggataaaa ttgcgaagga gagccagttt ctcaaggagg agcttgtggc 
     1501 tgctgtagaa gatgttcgaa aacaaggtga tttgatgaag gctgctgcag gagagttcgc 
     1561 agatgatccc tgctcttctg tgaagcgagg caacatggtt cgggcagctc gagctttgct 
     1621 ctctgctgtt acccggttgc tgattttggc tgacatggca gatgtctaca aattacttgt 
     1681 tcagctgaaa gttgtggaag atggtatctt gaagttgagg aatgctggca atgaacaaga 
     1741 cttaggaatc cagtataaag ccctaaaacc tgaagtggat aagctgaaca ttatggcagc 
     1801 caaaagacaa caggaattga aagatgttgg ccatcgtgat cagatggctg cagctagagg 
     1861 aatcctgcag aagaacgttc cgatcctcta tactgcatcc caggcatgcc tacagcaccc 
     1921 tgatgtcgca gcctataagg ccaaccggga cctgatatac aagcagctgc agcaggcggt 
     1981 cacaggcatt tccaatgcag cccaggccac tgcctcagac gatgcctcac agcaccaggg 
     2041 tggaggagga ggagaactgg catatgcact caataacttt gacaaacaaa tcattgtgga 
     2101 ccccttgagc ttcagcgagg agcgctttag gccttccctg gaggagcgtc tggaaagcat 
     2161 cattagtggg gctgccttga tggccgactc ctcctgcacg cgtgatgacc gtcgtgagcg 
     2221 aattgtggca gagtgtaatg ctgtccgcaa ggccctgcag gacctgcttt cggagtacat 
     2281 gggcaatgct ggacgtaaag aaagaagtga tgcactcaat tctgcaatag ataaaatgac 
     2341 caagaagacc agggacttgc gtagacagct ccgcaaagct gtcatggacc acgtttcaga 
     2401 ttctttcctg gaaaccaatg ttccactttt ggtattgatt gaagctgcaa agaatggaaa 
     2461 tgagaaagaa gttaaggagt atgcccaagt tttccgtgaa catgccaaca aattgattga 
     2521 ggttgccaac ttggcctgtt ccatctcaaa taatgaagaa ggtgtaaagc ttgttcgaat 
     2581 gtctgcaagc cagttagaag ccctctgtcc tcaggttatt aatgctgcac tggctttagc 
     2641 agcaaaacca cagagtaaac tggcccaaga gaacatggat ctttttaaag aacaatggga 
     2701 aaaacaagtc cgtgttctca cagatgctgt cgatgacatt acttccattg atgacttctt 
     2761 ggctgtctca gagaatcaca ttttggaaga tgtgaacaaa tgtgtcattg ctctccaaga 
     2821 gaaggatgtg gatggcctgg accgcacagc tggtgcaatt cgaggccggg cagcccgggt 
     2881 cattcacgta gtcacctcag agatggacaa ctatgagcca ggagtctaca cagagaaggt 
     2941 tctggaagcc actaagctgc tctccaacac agtcatgcca cgttttactg agcaagtaga 
     3001 agcagccgtg gaagccctca gctcggaccc tgcccagccc atggatgaga atgagtttat 
     3061 cgatgcttcc cgcctggtat atgatggcat ccgggacatc aggaaagcag tgctgatgat 
     3121 aaggacccct gaggagttgg atgactctga ctttgagaca gaagattttg atgtcagaag 
     3181 caggacgagc gtccagacag aagacgatca gctgatagct ggccagagtg cccgggcgat 
     3241 catggctcag cttccccagg agcaaaaagc gaagattgcg gaacaggtgg ccagcttcca 
     3301 ggaagaaaag agcaagctgg atgctgaagt gtccaaatgg gacgacagtg gcaatgacat 
     3361 cattgtgctg gccaagcaga tgtgcatgat tatgatggag atgacagact ttacccgagg 
     3421 taaaggacca ctcaaaaata catcggatgt catcagtgct gccaagaaaa ttgctgaggc 
     3481 aggatccagg atggacaagc ttggccgcac cattgcagac cattgccccg actcggcttg 
     3541 caagcaggac ctgctggcct acctgcaacg catcgccctc tactgccacc atctgaacat 
     3601 ctgcagcaag gtcaaggccg aggtgcagaa tctcggcggg gagcttgttg tctctggggt 
     3661 ggacagcgcc atgtccctga tccaggcagc caagaacttg atgaatgctg tggtgcagac 
     3721 agtgaaggca tcctacgtcg cctctaccaa ataccaaaag tcacagggta tggcttccct 
     3781 caaccttcct gctgtgtcat ggaagatgaa ggcaccagag aaaaagccat tggtgaagag 
     3841 agagaaacag gatgagacac agaccaagat taaacgggca tctcagaaga agcacgtgaa 
     3901 cccggtgcag gccctcagcg agttcaaagc tatggacagc atctaagtct gcccaggccg 
     3961 gccgccccca cccctcgggg ctcctgaata tcagtcactg ttcgtcactc aaatgaattt 
     4021 gctaaataca acactgatac tagattccac agggaaatgg gcagactgaa ccagtccagg 
     4081 tggtgaacac ccagctttct tgtacaaagt ggttgatggc cgcataacta gcataacccc 
     4141 ttggggcctc taaacgggtc ttgaggggtt ttttgcgcgc ttgcagccaa gctaattccg 
     4201 ggcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt 
     4261 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc 
     4321 tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac 
     4381 ctctaccggc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 
     4441 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 
     4501 gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 
     4561 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 
     4621 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 
     4681 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 
     4741 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 
     4801 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 
     4861 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 
     4921 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 
     4981 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 
     5041 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 
     5101 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 
     5161 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 
     5221 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 
     5281 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 
     5341 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 
     5401 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 
     5461 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 
     5521 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat ggcagggcct 
     5581 gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg gtggggtggg 
     5641 gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt atcgacagag 
     5701 tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc gtgcgtttta 
     5761 ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt 
     5821 tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc gcgctggagg 
     5881 atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg 
     5941 gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggttca tttcgaaccc 
     6001 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg 
     6061 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca 
     6121 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca 
     6181 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg 
     6241 tcatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt 
     6301 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct 
     6361 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta 
     6421 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca 
     6481 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc 
     6541 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc 
     6601 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg 
     6661 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg 
     6721 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct 
     6781 gcgtgcaatc catcttgttc aatcatactc ttcctttttc aatattattg aagcatttat 
     6841 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 
     6901 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt 
     6961 tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat 
     7021 ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc 
     7081 atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc 
     7141 tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat 
     7201 acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct 
     7261 tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca 
     7321 ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa 
     7381 aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt 
     7441 caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa 
     7501 cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta 
     7561 ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac 
     7621 tctatgaata gttcttacta caattttttt gtctaaagag taatactaga gataaacata 
     7681 aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt 
     7741 atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga 
     7801 agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa 
     7861 agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag 
     7921 agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa 
     7981 tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg 
     8041 cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac 
     8101 ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc 
     8161 attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg 
     8221 ccactcctca attggattag tctcatcctt caatgctatc atttcctttg atattggatc 
     8281 atattaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 
     8341 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 
     8401 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 
     8461 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 
     8521 gagagtgcac catagatcaa cgacattact atatatataa tataggaagc atttaataga 
     8581 acagcatcgt aatatatgtg tactttgcag ttatgacgcc agatggcagt agtggaagat 
     8641 attctttatt gaaaaatagc ttgtcacctt acgtacaatc ttgatccgga gcttttcttt 
     8701 ttttgccgat taagaattaa ttcggtcgaa aaaagaaaag gagagggcca agagggaggg 
     8761 cattggtgac tattgagcac gtgagtatac gtgattaagc acacaaaggc agcttggagt 
     8821 atgtctgtta ttaatttcac aggtagttct ggtccattgg tgaaagtttg cggcttgcag 
     8881 agcacagagg ccgcagaatg tgctctagat tccgatgctg acttgctggg tattatatgt 
     8941 gtgcccaata gaaagagaac aattgacccg gttattgcaa ggaaaatttc aagtcttgta 
     9001 aaagcatata aaaatagttc aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa 
     9061 cctaaggagg atgttttggc tctggtcaat gattacggca ttgatatcgt ccaactgcat 
     9121 ggagatgagt cgtggcaaga ataccaagag ttcctcggtt tgccagttat taaaagactc 
     9181 gtatttccaa aagactgcaa catactactc agtgcagctt cacagaaacc tcattcgttt 
     9241 attcccttgt ttgattcaga agcaggtggg acaggtgaac ttttggattg gaactcgatt 
     9301 tctgactggg ttggaaggca agagagcccc gaaagcttac attttatgtt agctggtgga 
     9361 ctgacgccag aaaatgttgg tgatgcgctt agattaaatg gcgttattgg tgttgatgta 
     9421 agcggaggtg tggagacaaa tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa 
     9481 aatgctaaga aataggttat tactgagtag tatttattta agtattgttt gtgcacttgc 
     9541 cgatctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa 
     9601 ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt 
     9661 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag 
     9721 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 
     9781 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat 
     9841 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 
     9901 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 
     9961 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac 
    10021 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg 
    10081 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagatccttt tgttgtttcc 
    10141 gggtgtacaa tatggacttc ctcttttctg gcaaccaaac ccatacatcg ggattcctat 
//
