LOCUS       peGBKT7-haNCTN    10227 bp    dsDNA    circular
DEFINITION  LMBP 8895.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        240..946
                     /label=truncated\ADH1\promoter
     CDS             972..1412
                     /label=GAL4DNABD
     promoter        1422..1445
                     /label=T7\RNA\polymerase\promoter
     CDS             1458..1490
                     /label=c-Myc\epitope\tag
     terminator      4468..4514
                     /label=T7\transcription\terminator
     terminator      4547..4743
                     /label=ADH1\transcription\terminator
     CDS             complement(6355..7277)
                     /label=KAN
     CDS             9164..9838
                     /label=TRP1
     primer_bind     1364..1383
                     /label=FVR175
     primer_bind     complement(4551..4570)
                     /label=FVR217
     5'clip          1518..1542
                     /label=attB1
     primer_bind     1518..1542
                     /label=FVR\2923
     3'clip          4431..4455
                     /label=attB2
     primer_bind     4431..4455
                     /label=FVR2924
ORIGIN
        1 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 
       61 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 
      121 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat 
      181 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccaga 
      241 tccttttgtt gtttccgggt gtacaatatg gacttcctct tttctggcaa ccaaacccat 
      301 acatcgggat tcctataata ccttcgttgg tctccctaac atgtaggtgg cggaggggag 
      361 atatacaata gaacagatac cagacaagac ataatgggct aaacaagact acaccaatta 
      421 cactgcctca ttgatggtgg tacataacga actaatactg tagccctaga cttgatagcc 
      481 atcatcatat cgaagtttca ctaccctttt tccatttgcc atctattgaa gtaataatag 
      541 gcgcatgcaa cttcttttct ttttttttct tttctctctc ccccgttgtt gtctcaccat 
      601 atccgcaatg acaaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca 
      661 gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt 
      721 tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc 
      781 cagttacttg aatttgaaat aaaaaaagtt tgctgtcttg ctatcaagta taaatagacc 
      841 tgcaattatt aatcttttgt ttcctcgtca ttgttctcgt tccctttctt ccttgtttct 
      901 ttttctgcac aatatttcaa gctataccaa gcatacaatc aactccaagc ttgaagcaag 
      961 cctcctgaaa gatgaagcta ctgtcttcta tcgaacaagc atgcgatatt tgccgactta 
     1021 aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg 
     1081 agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat ctgacagaag 
     1141 tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct cgagaagacc 
     1201 ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta acaggattat 
     1261 ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca gtggagactg 
     1321 atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg gaagagagta 
     1381 gtaacaaagg tcaaagacag ttgactgtat cgccggaatt tgtaatacga ctcactatag 
     1441 ggcgagccgc catcatggag gagcagaagc tgatctcaga ggaggacctg catatggcca 
     1501 tggaggccga attatcaaca agtttgtaca aaaaagcagg cttgacttcg gcaacttcac 
     1561 ctatcattct gaaatgggac cccaaaagtt tggaaatccg gacgctaaca gtggaaaggc 
     1621 tgttggagcc acttgttaca caggtgacta cacttgtcaa cacaagcaac aaaggcccat 
     1681 ctggtaaaaa gaaagggagg tcaaagaaag cccatgtact agctgcctct gtagagcaag 
     1741 ccactcagaa tttcctggaa aagggtgaac agatcgctaa ggagagtcaa gatctcaaag 
     1801 aagagttggt ggctgctgta gaggatgtgc gcaaacaagg tgagacgatg cggatcgcct 
     1861 cctccgagtt tgcagatgac ccttgctcgt cggtaaagcg cggcaccatg gtacgggcgg 
     1921 caagggcttt gctctccgcg gtgacacgct tactcatcct ggcggacatg gcagatgtca 
     1981 tgagactttt atcccatctg aaaattgtgg aagaggccct ggaagctgtc aaaaatgcta 
     2041 caaatgagca agaccttgca aaccgtttta aagagtttgg gaaaaagatg gtgaaactta 
     2101 actatgtagc agcaagaaga caacaggagc tgaaggatcc tcactgtcgg gatgagatgg 
     2161 cagccgcccg aggggctctg aagaagaatg ccacaatgct gtacacggcc tctcaagcat 
     2221 ttctccgcca cccagatgtc gccgctacga gagccaaccg agattatgtg ttcaaacaag 
     2281 tccaggaggc catcgccggc atctccaatg ctgctcaagc tacctcgccc actgacgaag 
     2341 ccaagggcca cacgggcatc ggcgagctgg ctgcggctct taatgagttt gacaataaga 
     2401 ttatcctgga ccccatgacg ttcagcgagg ccaggttccg gccgtccctg gaggagaggc 
     2461 tggagagcat catcagcggc gcagcgctga tggccgactc ctcctgcacg cgagacgacc 
     2521 ggcgcgagag gatcgtggcg gagtgcaacg ccgtgcggca ggcgctccag gacctgctca 
     2581 gcgagtacat gaataatact ggaaggaaag aaaaaggaga tcctctcaac attgcgattg 
     2641 ataagatgac taagaaaaca agagatctaa ggagacagct tcggaaagca gtgatggatc 
     2701 acatatctga ctctttcctg gaaaccaatg ttcctttgct agttctcatt gaggctgcaa 
     2761 agagcggaaa tgaaaaggaa gtgaaagaat atgcccaagt tttccgtgag catgccaaca 
     2821 aactggtaga ggttgccaat ttggcctgtt ccatctccaa caatgaagaa ggggtgaaat 
     2881 tagttcggat ggcagccacc cagattgaca gcctgtgtcc ccaggtcatc aatgccgctc 
     2941 tgacactggc tgcccggcca cagagcaaag ttgctcagga taacatggac gtcttcaaag 
     3001 accagtggga gaagcaggtc cgagtgttga cagaggccgt ggatgacatc acctcagtgg 
     3061 atgacttcct ctctgtctca gaaaatcaca tcttggagga tgtgaacaag tgtgtgatag 
     3121 ccctccaaga gggcgatgtg gacactctgg accggactgc aggggccatc aggggccggg 
     3181 cagctcgagt catacacatc atcaatgctg agatggagaa ctatgaagct ggggtttata 
     3241 ctgagaaggt gttggaagct acaaaattgc tttctgaaac agtgatgcca cgcttcgctg 
     3301 aacaagtaga ggttgccatt gaagccctga gtgccaacgt tcctcaaccg tttgaggaga 
     3361 atgagttcat cgatgcctct cgcctggtgt atgatggcgt tcgggacatc agaaaggctg 
     3421 tgctgatgat caggacccca gaagaactag aggatgattc tgactttgag caggaagatt 
     3481 atgatgtgcg tagagggaca agtgttcaga ctgaggatga ccagctcatt gcagggcaga 
     3541 gcgcacgggc catcatggcg caactaccgc aggaggagaa ggcaaaaata gctgagcagg 
     3601 tggagatatt ccatcaagag aaaagcaagc tggatgcaga agtggccaaa tgggacgaca 
     3661 gcggcaatga tatcattgta ctggccaagc agatgtgtat gatcatgatg gaaatgacag 
     3721 acttcacaag aggcaaaggc ccattgaaaa atacatctga tgtcattaat gctgccaaga 
     3781 aaattgccga agcaggttct cgaatggaca aattagctcg tgctgtggct gatcagtgtc 
     3841 ctgattcagc atgtaagcag gatttattag cctaccttca acgaattgcc ttgtattgcc 
     3901 atcagcttaa tatctgcagc aaggtgaagg cagaagtgca gaatctggga ggagagctca 
     3961 ttgtgtcagg gacaggagtt cagagcactt tcactacctt ttatgaggta gattgtgatg 
     4021 tcatagatgg gggcagggct agtcaacttt ctacccacct cccaacctgt gctgagggag 
     4081 ctccgatcgg gagtggaagc agtgattcct ccatgctgga cagtgccaca tcgcttatcc 
     4141 aggcagctaa aaacctgatg aatgctgttg tcctcacggt gaaagcatcc tatgtggcct 
     4201 caaccaaata ccagaaggtc tatgggacag cagctgtcaa ctcacctgtt gtgtcttgga 
     4261 agatgaaggc tccagagaag aagccccttg tgaagagaga aaagcctgaa gaattccaga 
     4321 cacgagttcg acgaggttct cagaagaaac acattttcgc ctgtacaggc tttaagtgaa 
     4381 ttcaaagcaa tggattcctt ctaggacgat aggttttaac aagaaagctt acccagcttt 
     4441 cttgtacaaa gtggtatgat ggccgcataa ctagcataac cccttggggc ctctaaacgg 
     4501 gtcttgaggg gttttttgcg cgcttgcagc caagctaatt ccgggcgaat ttcttatgat 
     4561 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg 
     4621 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt 
     4681 tgctttctca ggtatagcat gaggtcgctc ttattgacca cacctctacc ggcatgcaag 
     4741 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 
     4801 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgaggta 
     4861 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 
     4921 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 
     4981 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 
     5041 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 
     5101 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 
     5161 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 
     5221 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 
     5281 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 
     5341 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 
     5401 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 
     5461 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 
     5521 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 
     5581 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 
     5641 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 
     5701 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 
     5761 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 
     5821 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 
     5881 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc 
     5941 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg 
     6001 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg 
     6061 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc 
     6121 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct 
     6181 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc 
     6241 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg 
     6301 tgatggcagg ttgggcgtcg cttggtcggt tcatttcgaa ccccagagtc ccgctcagaa 
     6361 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta 
     6421 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc 
     6481 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga 
     6541 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgtcatggg tcacgacgag 
     6601 atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc 
     6661 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc 
     6721 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg 
     6781 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga 
     6841 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac 
     6901 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc 
     6961 ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg 
     7021 cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca 
     7081 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg 
     7141 ttcaatcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 
     7201 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 
     7261 tccccgaaaa gtgccacctg aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca 
     7321 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat 
     7381 gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa 
     7441 atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca 
     7501 gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta 
     7561 caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct 
     7621 cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg 
     7681 ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt 
     7741 cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc 
     7801 cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt 
     7861 gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact 
     7921 acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta 
     7981 ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga 
     8041 gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac 
     8101 agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatat 
     8161 tttagtagct cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg 
     8221 cttttggttt tcaaaagcgc tctgaagttc ctatactttc tagagaatag gaacttcgga 
     8281 ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc 
     8341 acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat atatatatac 
     8401 atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg cgtctattta 
     8461 tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat gcggggtatc 
     8521 gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc tcaattggat 
     8581 tagtctcatc cttcaatgct atcatttcct ttgatattgg atcatattaa gaaaccatta 
     8641 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt 
     8701 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 
     8761 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 
     8821 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatagat 
     8881 caacgacatt actatatata taatatagga agcatttaat agaacagcat cgtaatatat 
     8941 gtgtactttg cagttatgac gccagatggc agtagtggaa gatattcttt attgaaaaat 
     9001 agcttgtcac cttacgtaca atcttgatcc ggagcttttc tttttttgcc gattaagaat 
     9061 taattcggtc gaaaaaagaa aaggagaggg ccaagaggga gggcattggt gactattgag 
     9121 cacgtgagta tacgtgatta agcacacaaa ggcagcttgg agtatgtctg ttattaattt 
     9181 cacaggtagt tctggtccat tggtgaaagt ttgcggcttg cagagcacag aggccgcaga 
     9241 atgtgctcta gattccgatg ctgacttgct gggtattata tgtgtgccca atagaaagag 
     9301 aacaattgac ccggttattg caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag 
     9361 ttcaggcact ccgaaatact tggttggcgt gtttcgtaat caacctaagg aggatgtttt 
     9421 ggctctggtc aatgattacg gcattgatat cgtccaactg catggagatg agtcgtggca 
     9481 agaataccaa gagttcctcg gtttgccagt tattaaaaga ctcgtatttc caaaagactg 
     9541 caacatacta ctcagtgcag cttcacagaa acctcattcg tttattccct tgtttgattc 
     9601 agaagcaggt gggacaggtg aacttttgga ttggaactcg atttctgact gggttggaag 
     9661 gcaagagagc cccgaaagct tacattttat gttagctggt ggactgacgc cagaaaatgt 
     9721 tggtgatgcg cttagattaa atggcgttat tggtgttgat gtaagcggag gtgtggagac 
     9781 aaatggtgta aaagactcta acaaaatagc aaatttcgtc aaaaatgcta agaaataggt 
     9841 tattactgag tagtatttat ttaagtattg tttgtgcact tgccgatcta tgcggtgtga 
     9901 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt 
     9961 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 
    10021 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 
    10081 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 
    10141 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 
    10201 aggtgccgta aagcactaaa tcggaac 
//
