LOCUS       pEGFP-C2-p120-3AC 7596 bp dsDNACircular
DEFINITION  LMBP 8897.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        3363..3951
                     /note="Human cytomegalovirus immediate early promoter"
                     /label=CMV
     polyA_signal    181..231
                     /note="SV40 early mRNA polyadenylation signal"
                     /label=SV40\poly-A\signal
     rep_origin      278..733
                     /note="f1 single stranded origin"
                     /label=f1\origin
     rep_origin      1074..1209
                     /note="SV40 origin of replication"
                     /label=SV40\origin
     promoter        907..1175
                     /note="SV40 early promoter"
                     /label=SV40\\promoter
     misc_difference 1258..2052
                     /note="Kanamycin/neomycin resistance gene"
                     /label=Kan/neo
     polyA_signal    2288..2306
                     /note="Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal"
                     /label=poly-A\signal
     promoter        2637..3280
                     /note="pUC plasmid replication origin"
                     /label=ORI
     3'UTR           7296..7564
                     /label=3'UTR
     5'clip          4779..4781
                     /note="Start codon"
                     /label=START
     3'clip          7293..7295
                     /label=STOP
     CDS             4776..7295
                     /note="hCAS cDNA met derde ATG en de exons AC"
                     /label=hCAS
ORIGIN
        1 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata 
       61 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga 
      121 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca 
      181 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 
      241 gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg 
      301 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 
      361 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 
      421 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 
      481 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 
      541 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 
      601 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 
      661 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 
      721 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 
      781 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 
      841 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc 
      901 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 
      961 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 
     1021 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 
     1081 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     1141 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 
     1201 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg 
     1261 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 
     1321 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 
     1381 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa 
     1441 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 
     1501 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 
     1561 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 
     1621 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 
     1681 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 
     1741 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc 
     1801 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 
     1861 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 
     1921 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 
     1981 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 
     2041 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc 
     2101 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 
     2161 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 
     2221 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta 
     2281 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg 
     2341 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat 
     2401 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc 
     2461 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt 
     2521 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 
     2581 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
     2641 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     2701 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     2761 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 
     2821 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     2881 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     2941 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     3001 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     3061 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     3121 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     3181 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     3241 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     3301 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc 
     3361 attagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc 
     3421 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 
     3481 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 
     3541 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 
     3601 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 
     3661 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 
     3721 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg 
     3781 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa 
     3841 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt 
     3901 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc 
     3961 taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 
     4021 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 
     4081 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 
     4141 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc 
     4201 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 
     4261 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 
     4321 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 
     4381 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 
     4441 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 
     4501 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 
     4561 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 
     4621 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 
     4681 agctgtacaa gtccggccgg actcagatct cgagctcaag cttcgaattc aacggacccc 
     4741 aggatcacag tcaccttcta tatagcacca tccccaggat gcaggagccg gggcagattg 
     4801 tggagaccta cacggaggag gatcctgagg gagccatgtc tgtagtctct gtggagacct 
     4861 cagatgatgg gaccactcgg cgcacagaga ccacggtcaa gaaagtagtg aagactgtga 
     4921 caacacggac agtacagcca gtcgctatgg gaccagacgg gttgcctgtg gatgcttcat 
     4981 cagtttctaa caactatatc cagactttgg gtcgtgattt ccgcaagaat ggcaatgggg 
     5041 gacctggtcc ctatgtgggg caagctggca ctgctaccct tcctaggaac ttccactacc 
     5101 ctcctgatgg ttatagtcgc cactatgaag atggttatcc aggtggcagt gataactatg 
     5161 gcagtctgtc ccgggtgacc cgcattgagg agcggtatag gcccagcatg gaaggctacc 
     5221 gggcacctag tagacaggat gtgtatgggc cccaacccca ggttcgggta ggtgggagca 
     5281 gcgtggatct gcatcgcttt catccagagc cttatgggct agaggatgac cagcgtagta 
     5341 tgggctatga tgacctggat tatggtatga tgtctgatta tggcactgcc cgtcggactg 
     5401 ggacaccctc tgaccctcgt cggcgcctca ggagctatga agacatgatt ggtgaggagg 
     5461 tgccatcgga tcaatactac tgggctcctt tggcccagca tgagcgagga agtttagcaa 
     5521 gcttggatag cctgcgcaaa ggagggcctc cacctcctaa ttggagacag ccagagctgc 
     5581 cagaggtgat cgccatgctt ggattccgct tggatgctgt caagtccaat gcagctgcat 
     5641 acctgcaaca cttatgctac cgcaatgaca aggtgaagac tgacgtgcgg aagctcaagg 
     5701 gcatcccagt actggtggga ttgttagacc atcccaaaaa ggaagtgcac cttggagcct 
     5761 gtggagctct caagaatatc tcttttggac gtgaccagga taacaagatt gccataaaaa 
     5821 actgtgatgg tgtgcctgcc cttgtgcgat tgcttcgaaa ggctcgtgat atggacctta 
     5881 ctgaagttat taccggaacc ctgtggaatc tttcatccca tgactcaatc aaaatggaga 
     5941 ttgtggacca tgcactgcat gccttgacag atgaagtgat cattcctcat tctggttggg 
     6001 agcgggaacc taatgaagac tgtaagccac gccatattga gtgggaatcg gtgctcacca 
     6061 acacagctgg ctgccttagg aatgtaagct cagagaggag tgaagctcgc cggaaacttc 
     6121 gggaatgtga tggtttagtt gatgccctca ttttcattgt tcaggctgag attgggcaga 
     6181 aggattcaga cagcaagctt gtagagaact gtgtttgcct tcttcggaac ttatcatatc 
     6241 aagttcaccg ggagatccca caggcagagc gttaccaaga ggcagctccc aatgttgcca 
     6301 acaatactgg gccacatgct gccagttgct ttggggccaa gaagggcaaa gatgagtggt 
     6361 tctccagagg gaaaaaacct atagaggatc cagcaaacga tacagtggat ttccctaaaa 
     6421 gaacgagtcc agctcgaggc tatgagctct tatttcagcc agaggtggtt cggatataca 
     6481 tctcacttct taaggagagc aagactcctg ccatcctaga agcctcagct ggagctatcc 
     6541 agaacttgtg tgctgggcgc tggacgtatg gtcgatacat ccgctctgct ctgcgtcaag 
     6601 agaaggctct ttctgccata gctgacctcc tgactaatga acatgaacgg gtggtgaaag 
     6661 ctgcatctgg agcactgaga aacctggctg tggatgctcg caacaaagaa ttaattggta 
     6721 aacatgctat tcctaacttg gtaaagaatc tgccaggagg acagcagaac tcctcttgga 
     6781 atttctctga ggacactgtc atctctattt tgaacactat caacgaggtt atcgctgaga 
     6841 acttggaggc tgccaaaaag cttcgagaga cacagggtat tgagaagctg gtgttgatca 
     6901 acaaatcagg gaaccgctca gaaaaagaag ttcgagcagc agcacttgta ttacagacaa 
     6961 tctggggata taaggaactg cggaagccac tggaaaaaga aggatggaag aaatcagact 
     7021 ttcaggtgaa tctaaacaat gcttcccgaa gccagagcag tcattcatat gatgatagta 
     7081 ctctccctct cattgaccgg aaccaaaaat cagataagaa acctgatcgg gaagaaattc 
     7141 agatgagcaa tatgggatca aacacaaaat cactagataa caactattcc acaccaaatg 
     7201 agagaggaga ccacaataga acactggatc gatcggggga tctaggcgac atggagccat 
     7261 tgaagggaac aacacccttg atgcagaaga tttagcacca ctatctccgt tccatctggg 
     7321 cttatatgta cttttatttt ttggtggtga aattgactga tgattttcct ttttcttcgc 
     7381 tggactattg tgccaactgc caggctgcct cctgccctta cagccctaag tggctgcctt 
     7441 ctttccatca actcccaact tcttcctgtg aagtttaatt gtctcaacgc ctccccctcc 
     7501 cccattccct ccatttttct cccaagaaac ctgactcaat tatttgcata ttttgagaaa 
     7561 ctgcgacctc gagggatccg ggccctctag gatccg 
//
