LOCUS       pdEGFP-aTctn-V583A        7533 bp    DNA     circular      1-APR-2014
DEFINITION  LMBP 8903.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8903
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 01-APR-2014
            
            
COMMENT     VNTDATE|694089839|
COMMENT     VNTNAME|pdEGFP-aTctn-V583A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Created: 04/01/14 10:43" 1) 
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FEATURES             Location/Qualifiers
     promoter        6107..6674
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      complement(3022..3477)
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      3867..3953
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      5774..6100
                     /vntifkey="33"
                     /label=pMB1\ori
     attenuator      7508..7532
                     /vntifkey="0"
                     /label=attB1
     CDS             6719..7436
                     /vntifkey="4"
                     /label=EGFP
     attenuator      complement(2738..2762)
                     /vntifkey="0"
                     /label=attB2
     terminator      2872..2968
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(2872..2968)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        3629..3910
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             4002..4796
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4987..5056
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     CDS             2..2686
                     /vntifkey="4"
                     /label=hCTNNA3\mutant
     mutation        1745..1747
                     /vntifkey="62"
                     /label=V583A
                     /note="WT: GTA"
BASE COUNT     2026 a      1881 c      1890 g      1736 t 
ORIGIN
        1 gtcagctgaa acaccaatca cattgaatat cgatcctcag gatctgcagg tccaaacatt 
       61 caccgtggag aagctactgg agcctctcat aatccaggtt accacacttg taaactgtcc 
      121 ccagaaccct tccagcagga aaaaaggacg ttcgaaaaga gccagtgtcc ttctagcttc 
      181 tgtggaggaa gcaacttgga atttattaga caagggagag aagattgccc aggaagctac 
      241 agttttaaag gatgagctta cggcttcact tgaggaagtt cgcaaagaaa gtgaagctct 
      301 gaaagtatca gctgagagat ttacagatga cccctgtttt ctcccaaaaa gggaggctgt 
      361 ggttcaagct gcccgtgcct tgctggctgc ggtgacgaga ctccttatcc ttgcggacat 
      421 gattgatgtc atgtgcctct tgcaacatgt gtcagctttt caaaggacat ttgagtctct 
      481 caaaaatgtt gccaacaaat ctgacctcca gaaaacctac cagaagcttg ggaaggagct 
      541 ggaaaatttg gattatttag ccttcaaacg tcagcaggac ttaaaatctc caaatcagag 
      601 agatgaaatt gcaggagccc gagcttcact gaaggagaac tctcccctct tgcattcaat 
      661 ttgttcagct tgtttggagc attctgatgt tgcttccctc aaagcaagca aggacacagt 
      721 ttgtgaagaa attcagaatg ctctcaatgt aatttcaaat gcttcacaag ggatccagaa 
      781 tatgacaacc ccaccagaac ctcaggcagc aaccctggga agtgcccttg atgagctgga 
      841 gaatttaatt gtcctgaatc cactcacagt aactgaggag gaaatacgac catcactaga 
      901 gaaacgcctt gaagccatta tcagtggggc tgctctgctg gcggattctt catgtacgag 
      961 ggacttacac cgagagcgga ttatcgcaga atgcaacgcc attcgccagg ctcttcagga 
     1021 tctgctttca gagtacatga acaacgctgg aaaaaaagaa aggagtaata ccctgaatat 
     1081 tgctttagac aacatgtgta agaagacaag agaccttcgc agacagctcc gcaaggctat 
     1141 tatagatcat gtgtcagact ctttcctgga tacgacagtc cctcttttgg ttctcattga 
     1201 agctgctaag aatggccggg aaaaggaaat aaaagaatat gctgcgatat ttcatgaaca 
     1261 caccagcagg cttgtagagg tggcaaatct tgcttgttcc atgtcaacaa atgaagatgg 
     1321 aattaaaatt gtcaaaattg cagccaatca tttggaaacc ttgtgtccac agattattaa 
     1381 tgctgcactt gctttggctg caagacccaa aagtcaagcg gtcaaaaaca ccatggaaat 
     1441 gtacaagcgt acatgggaga atcatataca tgtcctcact gaagccgtag atgacattac 
     1501 aagcattgat gacttccttg ctgtatctga aagccatatc ttggaagatg tcaacaagtg 
     1561 tatcatagcc ttaagagacc aggatgctga taatttagac cgtgctgcgg gtgctatcag 
     1621 aggccgggca gcaagagttg ctcacatcgt cacgggtgaa atggacagtt acgagccagg 
     1681 ggcttacacg gaaggtgtaa tgagaaatgt taacttcctt acaagtactg taattcctga 
     1741 atttgcaaca caagtgaatg ttgccttgga agccttaagc aaaagctcat tgaatgtgtt 
     1801 ggatgataat caatttgtgg acatctcaaa gaagatctat gatacaattc atgatatcag 
     1861 atgttcagtc atgatgattc ggaccccaga ggaactggag gatgtttctg accttgaaga 
     1921 ggaacacgag gtccgcagtc acaccagcat tcagaccgaa gggaaaactg atagggctaa 
     1981 gatgactcaa ctgcctgagg cagaaaaaga aaagattgct gagcaagttg ctgatttcaa 
     2041 gaaagtaaag agtaagctgg atgctgagat tgagatatgg gatgatacaa gcaacgacat 
     2101 cattgttctg gccaagaaca tgtgtatgat catgatggag atgacagact tcactagggg 
     2161 caaaggacca ctaaagcata caactgatgt gatctatgca gcgaaaatga tatcagaatc 
     2221 aggatcaagg atggatgtcc ttgctcggca gattgctaat cagtgcccag atccatcttg 
     2281 taaacaggac ttgttggcct acctggaaca gattaagttc tactcccacc aactgaaaat 
     2341 ctgcagtcaa gttaaagctg agatccagaa cctgggagga gagctcatca tgtcagcttt 
     2401 ggacagtgtc acatccctga tccaagcagc caaaaattta atgaatgctg tagtgcaaac 
     2461 agtgaaaatg tcttacattg cctcaaccaa gatcatccga atccagagtc ctgctgggcc 
     2521 ccggcaccca gttgtgatgt ggagaatgaa ggctcctgca aaaaaaccct tgattaaaag 
     2581 agagaagcca gaggaaacgt gtgcagctgt cagacgaggc tcagcaaaga aaaaaatcca 
     2641 tccattgcaa gtcatgagtg aatttagagg aagacaaatc tactgaaacc actattctac 
     2701 atatagtgcc tatatgacaa aatcctgcct aaccactacc cagctttctt gtacaaagtg 
     2761 gtgatgatcc accggatcta gataactgat cataatcagc cataccacat ttgtagaggt 
     2821 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 
     2881 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 
     2941 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 
     3001 catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 
     3061 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 
     3121 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 
     3181 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 
     3241 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 
     3301 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 
     3361 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 
     3421 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag 
     3481 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     3541 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     3601 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa 
     3661 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     3721 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 
     3781 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 
     3841 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 
     3901 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 
     3961 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 
     4021 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 
     4081 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 
     4141 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc 
     4201 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 
     4261 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 
     4321 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 
     4381 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 
     4441 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 
     4501 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga 
     4561 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 
     4621 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 
     4681 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 
     4741 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 
     4801 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 
     4861 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 
     4921 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct 
     4981 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag 
     5041 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg 
     5101 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc 
     5161 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg 
     5221 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact 
     5281 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 
     5341 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 
     5401 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 
     5461 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 
     5521 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 
     5581 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 
     5641 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 
     5701 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 
     5761 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 
     5821 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 
     5881 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 
     5941 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 
     6001 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 
     6061 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt 
     6121 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta 
     6181 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga 
     6241 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt 
     6301 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta 
     6361 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg 
     6421 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt 
     6481 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc 
     6541 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat 
     6601 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct 
     6661 atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat 
     6721 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 
     6781 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 
     6841 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 
     6901 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca 
     6961 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 
     7021 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 
     7081 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 
     7141 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 
     7201 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 
     7261 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 
     7321 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 
     7381 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg 
     7441 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 
     7501 gatcatcaca agtttgtaca aaaaagcagg ctt 
//
