LOCUS       pENTR207-hactl    5597 bp    dsDNA    circular
DEFINITION  LMBP 8908.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     CDS             complement(783..1316)
                     /label=Gm\R
     rep_origin      2205..2807
                     /label=pUC\ORI
     primer_bind     3140..3164
                     /label=FVR\2831
     primer_bind     complement(24..47)
                     /label=FVR\2832
     terminator      complement(5247..5513)
                     /label=T1-T2\rrnB
     CDS             3596..4405
                     /label=Kana
     rep_origin      4526..5165
                     /label=ori
     primer_bind     5498..5522
                     /label=FVR2831
     primer_bind     complement(3497..3520)
                     /label=FVR2832
     insertion_seq   3361..3363
                     /label=stop
     3'clip          complement(3374..3473)
                     /label=att\L2
     CDS             3376..3378
                     /label=Pro
ORIGIN
        1 ccatccagct gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata 
       61 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acagggggtg 
      121 ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg 
      181 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc 
      241 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg 
      301 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc 
      361 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc 
      421 ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg 
      481 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta 
      541 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg 
      601 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa 
      661 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg 
      721 ataaccttat ttttgacgag gggaaattag gccgggaagc cgatctcggc ttgaacgaat 
      781 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac 
      841 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa 
      901 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc 
      961 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca 
     1021 aacctgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca 
     1081 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt 
     1141 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg 
     1201 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct 
     1261 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt 
     1321 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg 
     1381 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt 
     1441 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca 
     1501 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca 
     1561 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct 
     1621 ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt 
     1681 cttctacggc aaggtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg 
     1741 gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt 
     1801 tctggaaggc gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct 
     1861 actaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc 
     1921 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa 
     1981 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt 
     2041 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg 
     2101 ggacggcgca agctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 
     2161 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 
     2221 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 
     2281 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 
     2341 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 
     2401 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 
     2461 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 
     2521 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 
     2581 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 
     2641 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 
     2701 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 
     2761 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 
     2821 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 
     2881 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct 
     2941 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt 
     3001 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg 
     3061 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat 
     3121 gcctggcagt tccctactct cgcgttaacg ctagcatgga tctcgggccc cacagtaact 
     3181 aagacttctt tgcagaataa agtatttcta aaggttgaca agtgtattac gaagacaaga 
     3241 tccatgatgg ctctcttagt ccaacttctt tcactttgtt ataaactgct gaagaagctt 
     3301 cagatggaaa ataacggatg ggtctcagtt acaaataagg acactatgga tagtaaaact 
     3361 tgaagcggcc gcgacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta 
     3421 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca 
     3481 gctgcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat 
     3541 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag 
     3601 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata 
     3661 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta 
     3721 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga 
     3781 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat 
     3841 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa 
     3901 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct 
     3961 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga 
     4021 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag 
     4081 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa 
     4141 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct 
     4201 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga 
     4261 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca 
     4321 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca 
     4381 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag 
     4441 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagttt 
     4501 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 
     4561 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 
     4621 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 
     4681 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 
     4741 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 
     4801 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 
     4861 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 
     4921 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 
     4981 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 
     5041 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 
     5101 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 
     5161 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 
     5221 tctgtggata accgtattac cgctagccag gaagagtttg tagaaacgca aaaaggccat 
     5281 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc 
     5341 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac 
     5401 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag 
     5461 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatc 
     5521 tcgggccccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt 
     5581 gataagcaat gcttttt 
//
